##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552463_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1697689
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.72526770215275	31.0	31.0	34.0	30.0	34.0
2	31.884871139531445	33.0	31.0	34.0	30.0	34.0
3	31.947536916361006	33.0	31.0	34.0	30.0	34.0
4	35.55585681476407	37.0	35.0	37.0	33.0	37.0
5	35.528021327816816	37.0	35.0	37.0	33.0	37.0
6	35.54121868021764	37.0	35.0	37.0	33.0	37.0
7	35.8990368671765	37.0	35.0	37.0	35.0	37.0
8	35.9140378479215	37.0	35.0	37.0	35.0	37.0
9	37.64532137511641	39.0	37.0	39.0	35.0	39.0
10	37.06458897948917	39.0	37.0	39.0	33.0	39.0
11	36.871876415527225	39.0	37.0	39.0	33.0	39.0
12	36.637556702081476	39.0	35.0	39.0	32.0	39.0
13	36.57830262197611	39.0	35.0	39.0	32.0	39.0
14	37.665302655551166	39.0	36.0	41.0	33.0	41.0
15	37.678157188978666	39.0	36.0	41.0	33.0	41.0
16	37.68920573791784	39.0	36.0	41.0	33.0	41.0
17	37.63292511172541	39.0	36.0	41.0	33.0	41.0
18	37.62955464752378	39.0	36.0	41.0	33.0	41.0
19	37.63494845051125	39.0	36.0	41.0	33.0	41.0
20	37.53970662471159	39.0	36.0	41.0	32.0	41.0
21	37.4780339626398	39.0	36.0	41.0	32.0	41.0
22	37.455902700671324	39.0	36.0	41.0	32.0	41.0
23	37.38996895191051	39.0	35.0	41.0	32.0	41.0
24	37.39213896066948	39.0	35.0	41.0	32.0	41.0
25	37.32433207731216	39.0	35.0	41.0	32.0	41.0
26	37.259836165516774	39.0	35.0	41.0	32.0	41.0
27	37.20098793124065	39.0	35.0	41.0	32.0	41.0
28	37.12558778433506	39.0	35.0	41.0	32.0	41.0
29	37.08246092187674	39.0	35.0	41.0	31.0	41.0
30	36.96537469465844	39.0	35.0	41.0	31.0	41.0
31	36.88551907917174	39.0	35.0	41.0	31.0	41.0
32	36.801847099203684	39.0	35.0	40.0	31.0	41.0
33	36.71224058116651	39.0	35.0	40.0	31.0	41.0
34	36.608183242042564	39.0	35.0	40.0	30.0	41.0
35	36.53713960566394	39.0	35.0	40.0	30.0	41.0
36	36.45766391842086	39.0	35.0	40.0	30.0	41.0
37	36.36841141104171	38.0	35.0	40.0	30.0	41.0
38	36.30705270517745	38.0	35.0	40.0	30.0	41.0
39	36.27569772791129	38.0	35.0	40.0	30.0	41.0
40	36.14880405068302	38.0	35.0	40.0	30.0	41.0
41	36.058154938861	38.0	35.0	40.0	30.0	41.0
42	36.00862819986464	38.0	35.0	40.0	30.0	41.0
43	35.938299064198446	38.0	35.0	40.0	30.0	41.0
44	35.8648209418804	38.0	35.0	40.0	29.0	41.0
45	35.78412182678924	38.0	34.0	40.0	29.0	41.0
46	35.63576838867425	38.0	34.0	40.0	29.0	41.0
47	35.511146623439274	38.0	34.0	40.0	28.0	41.0
48	35.4465929861123	37.0	34.0	40.0	28.0	41.0
49	35.38553645573482	37.0	34.0	40.0	28.0	41.0
50	35.27345585675586	37.0	34.0	40.0	28.0	41.0
51	34.019720337470524	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	19.0
9	98.0
10	126.0
11	104.0
12	36.0
13	26.0
14	42.0
15	58.0
16	114.0
17	269.0
18	503.0
19	942.0
20	1555.0
21	2545.0
22	3795.0
23	5443.0
24	7924.0
25	10583.0
26	13607.0
27	17481.0
28	22088.0
29	27711.0
30	35169.0
31	44758.0
32	57559.0
33	77831.0
34	126027.0
35	148930.0
36	151359.0
37	211089.0
38	328354.0
39	401463.0
40	77.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.13965101970974	20.30071467742325	25.314118192436897	10.24551611043012
2	31.033834818980388	24.48410751321355	25.876824318234963	18.605233349571094
3	32.871156024454415	24.48228150149998	25.855736828123412	16.79082564592219
4	29.654135710368625	26.190780525761788	25.105481628260534	19.049602135609053
5	25.361653400593394	31.02806226582136	23.561677079842067	20.048607253743178
6	26.575067636062904	36.004474317734285	22.31433436866234	15.106123677540467
7	82.4533233118669	6.340148283931862	6.6860891482480005	4.5204392559532405
8	83.4892020858944	4.969697041095277	6.763606290669257	4.7774945823410535
9	77.13256079293676	7.53583253469864	8.973669500126348	6.357937172238261
10	41.758767359628294	26.055596755353893	16.200906055231552	15.984729829786257
11	32.74628038468765	25.4819345592744	21.618034869755295	20.153750186282647
12	28.278500950409647	23.566094850116837	27.813692613900425	20.341711585573094
13	24.990089468683603	24.659227926905338	29.283455332513785	21.067227271897266
14	21.682534315766905	27.064674389714487	28.75532562206623	22.497465672452375
15	21.174785252186943	25.44282256644179	31.98607047580564	21.396321705565626
16	23.719303123245776	24.670714129619736	29.48254951289665	22.127433234237838
17	23.577522149227566	24.552317886255963	27.600402665034647	24.269757299481824
18	24.491117042049517	24.561389041220153	28.64388000393476	22.30361391279557
19	24.312815833759892	26.338510763750016	26.563463626141182	22.785209776348907
20	27.01778712119829	26.215048810471174	26.30758637182664	20.459577696503896
21	25.653461853142712	26.985272331975995	26.413730665628393	20.947535149252897
22	23.335192723755647	25.116025373316315	25.76914853073796	25.779633372190077
23	23.053869112658443	26.64657661091048	26.516046225192014	23.783508051239068
24	23.81307766027818	24.23847948593647	28.17966070346218	23.768782150323176
25	22.4658933408887	26.417205978244546	26.135175523903374	24.98172515696338
26	21.754985748273093	26.653645043350103	26.84567079129334	24.74569841708346
27	23.329243459785626	26.986862729274915	26.041931119303946	23.641962691635513
28	20.70214273639047	27.422454878367002	27.26394528090834	24.611457104334185
29	23.033076140565203	25.721790033392455	25.511975397142823	25.73315842889952
30	23.786099809800263	26.90640040667048	25.690571123450763	23.616928660078496
31	25.583189853972076	27.5468003857008	23.269809723689086	23.600200036638043
32	24.32330067521201	27.133002569964226	23.646498269117604	24.897198485706156
33	23.60656162583371	28.537617902925682	23.18557757045018	24.670242900790427
34	23.548129250999448	25.39622981594391	26.199321548293003	24.85631938476364
35	21.898710541212203	26.313889057418642	25.69498889372553	26.092411507643625
36	23.22268684075823	30.85853769447761	23.656865303362395	22.261910161401765
37	24.451003687954625	27.897924767139333	25.12474310665852	22.52632843824752
38	22.672939507766145	28.881202623095277	24.58942715656401	23.856430712574564
39	24.127976325463614	26.163508157265554	25.362301340233696	24.346214177037137
40	23.90785355857286	24.408416382505866	28.356607128867537	23.327122930053736
41	20.91672856453685	26.657355970380912	26.994107872525536	25.431807592556705
42	22.337130063280142	26.15549726716731	26.817161447120174	24.690211222432374
43	22.816193071875944	25.247439313089735	26.920890693171717	25.0154769218626
44	22.84646952415902	24.902617617243205	25.324013997852372	26.926898860745403
45	23.61633962404186	24.38126182121696	25.511680879124505	26.490717675616676
46	23.580113907788764	25.398468152883126	26.30970690155853	24.71171103776958
47	21.44279664885618	25.971600216529644	28.439602306429506	24.14600082818467
48	21.4101051488229	25.289673196916514	27.96460364648649	25.335618007774098
49	23.134920471299512	24.821271740583818	28.150562323252377	23.893245464864297
50	21.365927446075222	24.16914994442445	27.658010389417615	26.80691222008271
51	21.17831946840676	24.06271113260438	25.950277112003434	28.808692286985426
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	363.0
1	770.0
2	1177.0
3	2070.5
4	2964.0
5	2277.5
6	1591.0
7	1595.5
8	1600.0
9	1730.0
10	1860.0
11	2137.5
12	2415.0
13	2482.5
14	2550.0
15	2539.5
16	2529.0
17	2643.0
18	2757.0
19	3268.5
20	3780.0
21	4203.5
22	4627.0
23	5024.5
24	5422.0
25	7305.5
26	10803.5
27	12418.0
28	15527.0
29	18636.0
30	21733.5
31	24831.0
32	27076.5
33	29322.0
34	32754.5
35	36187.0
36	39195.0
37	42203.0
38	47331.0
39	52459.0
40	58260.5
41	64062.0
42	72622.5
43	81183.0
44	90957.5
45	100732.0
46	118103.5
47	135475.0
48	153875.5
49	172276.0
50	172091.0
51	171906.0
52	154013.0
53	136120.0
54	122623.5
55	109127.0
56	100924.5
57	92722.0
58	88218.0
59	83714.0
60	78991.0
61	74268.0
62	70267.5
63	66267.0
64	56700.0
65	47133.0
66	39739.5
67	32346.0
68	28280.0
69	24214.0
70	20499.0
71	16784.0
72	14323.5
73	11863.0
74	9792.5
75	6456.0
76	5190.0
77	3981.5
78	2773.0
79	2227.5
80	1682.0
81	1203.0
82	724.0
83	534.0
84	344.0
85	234.0
86	124.0
87	74.0
88	24.0
89	23.5
90	23.0
91	14.0
92	5.0
93	4.0
94	3.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1697689.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.95934481338096
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.11093751662382	15.408231551934309
2	12.535030887556303	5.755921927875718
3	5.424794100560904	3.7364915488911783
4	3.0085182281811758	2.7629442951261423
5	1.7323988612721948	1.9887371405128422
6	1.1637391703632314	1.603121333112439
7	0.7786838789394157	1.2514650174033728
8	0.6118993606933811	1.1239046730597364
9	0.4651815516290273	0.9612237280206973
>10	5.274791737116899	31.1592686162835
>50	1.748624254848289	26.713942424110847
>100	0.1369080367691553	5.21353687979071
>500	0.0056616102974522756	0.8960821656972487
>1k	0.002573459226114671	1.1237955157300326
>5k	2.5734592261146707E-4	0.3013331824512705
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGC	5100	0.3004083786841995	TruSeq Adapter, Index 15 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGCT	3661	0.21564609301232437	TruSeq Adapter, Index 14 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGC	3314	0.19520654254106612	TruSeq Adapter, Index 15 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGC	2204	0.12982354247450506	TruSeq Adapter, Index 15 (95% over 23bp)
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	2016	0.11874966498575416	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1780720732713707E-4	0.0	0.0	0.24816088223461424	0.0
2	1.1780720732713707E-4	0.0	0.0	1.059793637114925	0.0
3	1.1780720732713707E-4	0.0	0.0	1.4609860816674904	0.0
4	1.1780720732713707E-4	0.0	0.0	1.9203163830359977	0.0
5	1.1780720732713707E-4	0.0	0.0	3.189217813156591	0.0
6	1.1780720732713707E-4	0.0	0.0	3.8828666498987743	0.0
7	1.1780720732713707E-4	0.0	0.0	4.631119127237085	0.0
8	1.1780720732713707E-4	0.0	0.0	5.946024271818926	0.0
9	1.1780720732713707E-4	0.0	0.0	6.4196092452740166	0.0
10	1.1780720732713707E-4	0.0	0.0	7.622833157309731	0.0
11	1.1780720732713707E-4	0.0	0.0	9.407906866334175	0.0
12	1.1780720732713707E-4	0.0	0.0	10.76215961816328	0.0
13	1.1780720732713707E-4	0.0	0.0	11.262251213266977	0.0
14	1.1780720732713707E-4	0.0	0.0	11.452156431478322	0.0
15	1.1780720732713707E-4	0.0	0.0	11.87355281208749	0.0
16	1.1780720732713707E-4	0.0	0.0	12.723237294934467	0.0
17	1.1780720732713707E-4	0.0	0.0	13.753166804991963	0.0
18	1.1780720732713707E-4	0.0	0.0	14.892185789034388	0.0
19	1.1780720732713707E-4	0.0	0.0	15.552554089706653	0.0
20	1.1780720732713707E-4	0.0	0.0	16.218105907501315	0.0
21	4.1232522564497976E-4	0.0	0.0	17.01560179750237	0.0
22	6.479396402992539E-4	0.0	0.0	17.873591688465908	0.0
23	6.479396402992539E-4	0.0	0.0	18.723983014556847	0.0
24	9.424576586170966E-4	0.0	0.0	19.417985272921012	0.0
25	0.0011780720732713706	0.0	0.0	20.042304568151174	0.0
26	0.0011780720732713706	0.0	0.0	20.585513601136604	0.0
27	0.0013547828842620762	0.0	0.0	21.119651479157845	0.0
28	0.0013547828842620762	0.0	0.0	21.655968790514635	0.0
29	0.0013547828842620762	0.0	0.0	22.230867962271063	0.0
30	0.0013547828842620762	0.0	0.0	22.925694871086517	0.0
31	0.0014136864879256447	0.0	0.0	23.524685616741348	0.0
32	0.0014725900915892134	0.0	0.0	24.11613670112724	0.0
33	0.0014725900915892134	0.0	0.0	24.730324576527266	0.0
34	0.0015903972989163503	0.0	0.0	25.29585807530119	0.0
35	0.0015903972989163503	0.0	0.0	25.925949923690382	0.0
36	0.001649300902579919	0.0	0.0	26.490482061202023	0.0
37	0.0017082045062434875	0.0	0.0	27.055662138353963	0.0
38	0.0018260117135706245	0.0	0.0	27.64870362003877	0.0
39	0.0018260117135706245	0.0	0.0	28.232968464777706	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATTAC	20	7.0342375E-4	45.000004	10
GCTCGAT	20	7.0342375E-4	45.000004	8
CGCTCGA	25	3.8915954E-5	45.0	24
ACGAGTA	25	3.8915954E-5	45.0	23
TAAGACG	80	0.0	42.187504	1
CGATAAC	80	0.0	42.187504	10
CTTACCG	60	3.6379788E-12	41.249996	1
CGTCAAT	50	1.0822987E-9	40.5	11
CGTAAGG	345	0.0	40.43478	2
TTAGCCG	45	1.9292202E-8	40.0	1
ATCGTCC	35	6.250053E-6	38.571426	19
TTACCGG	130	0.0	38.07692	2
TAGCGCG	185	0.0	37.7027	1
CGCATAA	30	1.14015136E-4	37.499996	34
CCCTCGT	385	0.0	37.4026	14
CGTGTAC	355	0.0	37.394363	18
GCGTAAG	230	0.0	37.173916	1
CACAACG	315	0.0	37.142857	12
TACGGGT	170	0.0	37.058823	4
ACGGGAT	365	0.0	36.9863	5
>>END_MODULE