FastQCFastQC Report
Sat 18 Jun 2016
SRR3552462_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552462_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1684833
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGCT37820.22447328607642417Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGC36230.21503614898331172No Hit
GCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGC35370.2099317855241439No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29860.17722824754738303No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATG253.891582E-545.01
TTACGCG502.1827873E-1145.01
CGTAAGG1800.042.5000042
TAATACG750.042.01
CGTTTTT24250.040.731961
GCGTACG501.0822987E-940.51
AATACGG1450.040.3448262
TATCGTG403.4594996E-739.3750041
TGTAACG900.037.5000041
TCGCACG601.5643309E-1037.4999961
ATTAGCG1200.037.4999961
ACCTCGC301.140148E-437.49999624
GCCCGTA301.140148E-437.4999969
GTCAACG552.750312E-936.818181
TATTGCG1800.036.2500041
TACGGGA6900.036.1956524
TCGTTAG504.881258E-836.01
TCACACG250.002107386536.01
ACTCGAC250.002107386536.012
TCGAGTA250.002107386536.017
Produced by FastQC (version 0.11.5)