##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552462_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1684833
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.801016480565135	31.0	31.0	34.0	30.0	34.0
2	31.977911757426405	33.0	31.0	34.0	30.0	34.0
3	31.99579542898317	33.0	31.0	34.0	30.0	34.0
4	35.621415297539876	37.0	35.0	37.0	33.0	37.0
5	35.60729817139147	37.0	35.0	37.0	33.0	37.0
6	35.629408968129184	37.0	35.0	37.0	33.0	37.0
7	35.98741715054252	37.0	35.0	37.0	35.0	37.0
8	36.01270155558444	37.0	35.0	37.0	35.0	37.0
9	37.74987906813316	39.0	38.0	39.0	35.0	39.0
10	37.13117323794109	39.0	37.0	39.0	33.0	39.0
11	36.980819464006224	39.0	37.0	39.0	33.0	39.0
12	36.8405533367402	39.0	37.0	39.0	33.0	39.0
13	36.84222115782395	39.0	37.0	39.0	33.0	39.0
14	37.98363695392956	40.0	37.0	41.0	33.0	41.0
15	37.973338603885374	40.0	37.0	41.0	33.0	41.0
16	37.98393728043076	40.0	37.0	41.0	33.0	41.0
17	37.95431713410172	40.0	37.0	41.0	33.0	41.0
18	37.9205547374725	40.0	37.0	41.0	33.0	41.0
19	37.896826569754985	40.0	37.0	41.0	33.0	41.0
20	37.805028747656294	40.0	36.0	41.0	33.0	41.0
21	37.77039267393267	39.0	36.0	41.0	33.0	41.0
22	37.7422326129652	39.0	36.0	41.0	33.0	41.0
23	37.69549623019017	39.0	36.0	41.0	33.0	41.0
24	37.697111820578066	39.0	36.0	41.0	33.0	41.0
25	37.63995956869316	39.0	36.0	41.0	33.0	41.0
26	37.58784461130569	39.0	36.0	41.0	33.0	41.0
27	37.53326828237576	39.0	36.0	41.0	33.0	41.0
28	37.47064427156875	39.0	36.0	41.0	32.0	41.0
29	37.41465237207486	39.0	36.0	41.0	32.0	41.0
30	37.322381506060246	39.0	36.0	41.0	32.0	41.0
31	37.239065236732664	39.0	36.0	41.0	32.0	41.0
32	37.13145991323769	39.0	35.0	41.0	31.0	41.0
33	37.0006944308427	39.0	35.0	41.0	31.0	41.0
34	36.898077732333114	39.0	35.0	41.0	31.0	41.0
35	36.84665839284962	39.0	35.0	41.0	31.0	41.0
36	36.73941215538869	39.0	35.0	41.0	31.0	41.0
37	36.63057169464273	39.0	35.0	40.0	30.0	41.0
38	36.56335375672248	39.0	35.0	40.0	30.0	41.0
39	36.485753187407894	39.0	35.0	40.0	30.0	41.0
40	36.4095943040052	39.0	35.0	40.0	30.0	41.0
41	36.35620919105929	39.0	35.0	40.0	30.0	41.0
42	36.28570131283041	39.0	35.0	40.0	30.0	41.0
43	36.19419016602833	38.0	35.0	40.0	30.0	41.0
44	36.09752717331629	38.0	35.0	40.0	30.0	41.0
45	35.99803363300695	38.0	35.0	40.0	29.0	41.0
46	35.85946737747896	38.0	35.0	40.0	29.0	41.0
47	35.74751800326798	38.0	34.0	40.0	29.0	41.0
48	35.647458828263694	38.0	34.0	40.0	28.0	41.0
49	35.55666407293779	38.0	34.0	40.0	28.0	41.0
50	35.43741664604148	38.0	34.0	40.0	28.0	41.0
51	34.11432587087266	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	17.0
9	82.0
10	108.0
11	103.0
12	56.0
13	37.0
14	34.0
15	66.0
16	93.0
17	185.0
18	392.0
19	711.0
20	1294.0
21	2083.0
22	3110.0
23	4647.0
24	6945.0
25	9979.0
26	12985.0
27	16259.0
28	19674.0
29	24605.0
30	31231.0
31	40177.0
32	52563.0
33	70980.0
34	109984.0
35	131261.0
36	149978.0
37	215347.0
38	344554.0
39	435226.0
40	66.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.40426202478228	20.860405749412553	26.082763098775963	10.652569127029206
2	32.28735429564829	23.829898868315137	26.241473190517993	17.641273645518577
3	32.168944934008294	24.397729626615813	26.876194851359152	16.557130588016737
4	28.90494191412443	26.302725552028004	26.461554349896993	18.330778183950574
5	25.656370690745018	30.151118834923107	24.983366303960093	19.20914417037178
6	25.87099136828398	35.89803855931122	23.38653148412929	14.844438588275516
7	82.8120650533317	5.8803454110882205	7.105036522907611	4.202553012672473
8	84.0520692555286	4.713226770843164	6.858246484963199	4.376457488665049
9	77.82949408042221	7.184688334096021	9.323594682677749	5.662222902804017
10	38.827646419556125	27.583802074152157	17.84574494920268	15.74280655708904
11	29.40873071693159	26.456390633374344	23.966114148998745	20.16876450069532
12	25.37242563506294	23.947417934002953	29.95566919688776	20.72448723404634
13	24.512815216701004	24.116099340409406	31.584970142441414	19.786115300448177
14	21.710282265363986	27.282466570870824	29.736775098778335	21.270476064986855
15	21.454114443389937	26.578539238013498	31.77258517609757	20.19476114249899
16	23.78663048503917	26.052314977211395	29.294832188115972	20.866222349633464
17	23.634508583343276	25.577549822445313	28.199115283235788	22.588826310975627
18	23.78704595648352	25.405603997547534	29.837437894438203	20.969912151530746
19	23.490339992153526	27.688560231192056	28.032273821797176	20.788825954857245
20	24.717523932639022	27.427050633504923	27.8447181412045	20.010707292651556
21	24.18310894907685	27.294693301947433	28.421748624344374	20.100449124631343
22	22.371178627199253	26.023884859805097	27.611163836415837	23.993772676579816
23	22.1863531875266	26.979231769558172	27.64042489671083	23.193990146204403
24	22.304762549166593	26.34338240051091	28.32167935931929	23.030175691003205
25	21.667607412722802	27.653067099231794	27.123578419938355	23.555747068107046
26	21.37274139336065	27.94514352461045	28.27140731455284	22.410707767476065
27	22.959604898527036	27.028257399991574	28.127357429490047	21.88478027199135
28	20.69510746762439	27.841809841094044	28.45991264416117	23.003170047120395
29	22.915446219298886	25.970704514928183	28.234489709069088	22.87935955670384
30	23.893584705427777	26.222717622458724	27.687254463795526	22.196443208317977
31	23.11985816991951	28.14302663824842	25.92678324795395	22.81033194387812
32	24.085591865781357	27.690637588413807	25.99248708922487	22.23128345657997
33	23.9208277615645	27.551098536175395	25.836091766958507	22.6919819353016
34	21.78560130291845	26.672079666055925	28.201904877219285	23.34041415380634
35	24.072000014244736	25.262978586008227	27.76316703198477	22.901854367762265
36	23.36100966683345	28.677026150366235	26.838030831542355	21.12393335125796
37	23.591358906194262	29.03569671296799	26.161880732393062	21.211063648444682
38	23.720985996831732	28.482822926663946	26.000024928286663	21.796166148217658
39	23.39674021104762	26.345697169986583	27.471446724986986	22.78611589397881
40	23.25625150979355	24.4963744181174	29.111075103585932	23.136298968503112
41	22.257814275954946	26.311331746232412	26.94836817654925	24.48248580126339
42	23.110421032826398	27.32448853981374	27.39808633852732	22.16700408883254
43	23.259812693602274	26.150484944205154	28.43676494940448	22.15293741278809
44	22.718453401613097	25.98637372368656	27.19397115322409	24.101201721476254
45	23.42291491204173	24.012884363019953	27.715150403630506	24.849050321307807
46	23.245983429811737	25.573632520255718	27.765778566777833	23.41460548315471
47	21.971851216114594	25.90636579411728	29.032788412857535	23.08899457691059
48	22.40358539985862	25.337229268420074	28.62568575045717	23.633499581264136
49	23.272098777742364	24.73259961076261	28.9052980325053	23.09000357898973
50	22.00194321929829	23.95477771387431	28.78926279340445	25.25401627342295
51	21.71799816361622	23.661336168035646	27.502369671059384	27.11829599728875
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	352.0
1	651.5
2	951.0
3	3315.5
4	5680.0
5	4135.0
6	2590.0
7	2533.0
8	2476.0
9	2572.0
10	2668.0
11	2863.0
12	3058.0
13	3124.5
14	3191.0
15	3362.5
16	3534.0
17	3531.5
18	3529.0
19	3700.5
20	3872.0
21	4691.0
22	5510.0
23	6005.5
24	6501.0
25	8800.0
26	12901.5
27	14704.0
28	17631.0
29	20558.0
30	24431.0
31	28304.0
32	31588.5
33	34873.0
34	38823.5
35	42774.0
36	47022.5
37	51271.0
38	56862.0
39	62453.0
40	68365.0
41	74277.0
42	81024.5
43	87772.0
44	98139.0
45	108506.0
46	125811.5
47	143117.0
48	161367.0
49	179617.0
50	179225.5
51	178834.0
52	158576.5
53	138319.0
54	121022.5
55	103726.0
56	91938.0
57	80150.0
58	74803.5
59	69457.0
60	64925.0
61	60393.0
62	54550.0
63	48707.0
64	40930.5
65	33154.0
66	28581.5
67	24009.0
68	20619.0
69	17229.0
70	13978.5
71	10728.0
72	9299.0
73	7870.0
74	6125.5
75	3275.0
76	2169.0
77	1769.0
78	1369.0
79	936.5
80	504.0
81	384.5
82	265.0
83	201.0
84	137.0
85	105.5
86	74.0
87	65.5
88	57.0
89	41.0
90	25.0
91	23.0
92	21.0
93	17.5
94	14.0
95	8.5
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1684833.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.44600290946573
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.02569311242902	21.015564533289027
2	12.357480609816495	7.5247178120027876
3	4.689275583150155	4.283090941436287
4	2.4209471896336576	2.9483266071699687
5	1.4339073535412676	2.182837372891087
6	0.9995318937147187	1.825905056648527
7	0.7251129228888873	1.545375311197638
8	0.6301385728852604	1.5348160658744976
9	0.49840232543302265	1.3656922785196464
>10	6.932488033632129	46.52276734704347
>50	0.23058477672381217	4.398711562323875
>100	0.05136021124242287	2.8410778399638037
>500	0.0029292778322422123	0.5795228395172128
>1k	0.0021481370769776222	1.431594432122238
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGCT	3782	0.22447328607642417	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGC	3623	0.21503614898331172	No Hit
GCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGC	3537	0.2099317855241439	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2986	0.17722824754738303	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.935306347869492E-5	0.0	0.0	0.25925418127493943	0.0
2	5.935306347869492E-5	0.0	0.0	0.9646653407192286	0.0
3	5.935306347869492E-5	0.0	0.0	1.348204836918555	0.0
4	5.935306347869492E-5	0.0	0.0	1.7813634941860708	0.0
5	5.935306347869492E-5	0.0	0.0	2.9249783212935645	0.0
6	5.935306347869492E-5	0.0	0.0	3.7514103771709126	0.0
7	5.935306347869492E-5	0.0	0.0	4.592680698917935	0.0
8	5.935306347869492E-5	0.0	0.0	5.986112570207255	0.0
9	5.935306347869492E-5	0.0	0.0	6.542903658700892	0.0
10	5.935306347869492E-5	0.0	0.0	7.901851400109091	0.0
11	5.935306347869492E-5	0.0	0.0	9.870533162633922	0.0
12	5.935306347869492E-5	0.0	0.0	11.3250986893063	0.0
13	5.935306347869492E-5	0.0	0.0	11.950323859990872	0.0
14	5.935306347869492E-5	0.0	0.0	12.232547676832066	0.0
15	5.935306347869492E-5	0.0	0.0	12.678882714191852	0.0
16	5.935306347869492E-5	0.0	0.0	13.582533105654981	0.0
17	5.935306347869492E-5	0.0	0.0	14.69368180703963	0.0
18	5.935306347869492E-5	0.0	0.0	15.847564714128938	0.0
19	5.935306347869492E-5	0.0	0.0	16.578972515376893	0.0
20	5.935306347869492E-5	0.0	0.0	17.274412360156763	0.0
21	1.1870612695738984E-4	0.0	0.0	18.11586074109422	0.0
22	1.1870612695738984E-4	0.0	0.0	19.053045613422814	0.0
23	1.1870612695738984E-4	0.0	0.0	19.972068448326926	0.0
24	5.341775713082543E-4	0.0	0.0	20.725199470808086	0.0
25	5.341775713082543E-4	0.0	0.0	21.38894477969033	0.0
26	5.341775713082543E-4	0.0	0.0	21.98888554533298	0.0
27	5.935306347869492E-4	0.0	0.0	22.591200433514775	5.935306347869492E-5
28	5.935306347869492E-4	0.0	0.0	23.23138257619598	5.935306347869492E-5
29	5.935306347869492E-4	0.0	0.0	23.878746439558103	5.935306347869492E-5
30	5.935306347869492E-4	0.0	0.0	24.60576211410864	5.935306347869492E-5
31	5.935306347869492E-4	0.0	0.0	25.26280052681779	5.935306347869492E-5
32	5.935306347869492E-4	0.0	0.0	25.906187734926846	5.935306347869492E-5
33	5.935306347869492E-4	0.0	0.0	26.559190139319448	5.935306347869492E-5
34	5.935306347869492E-4	0.0	0.0	27.190291263288408	5.935306347869492E-5
35	5.935306347869492E-4	0.0	0.0	27.82192656482868	5.935306347869492E-5
36	5.935306347869492E-4	0.0	0.0	28.45771658081246	5.935306347869492E-5
37	5.935306347869492E-4	0.0	0.0	29.081932749417895	5.935306347869492E-5
38	5.935306347869492E-4	0.0	0.0	29.713568050958166	5.935306347869492E-5
39	6.528836982656441E-4	0.0	0.0	30.329949615184415	5.935306347869492E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTATG	25	3.891582E-5	45.0	1
TTACGCG	50	2.1827873E-11	45.0	1
CGTAAGG	180	0.0	42.500004	2
TAATACG	75	0.0	42.0	1
CGTTTTT	2425	0.0	40.73196	1
GCGTACG	50	1.0822987E-9	40.5	1
AATACGG	145	0.0	40.344826	2
TATCGTG	40	3.4594996E-7	39.375004	1
TGTAACG	90	0.0	37.500004	1
TCGCACG	60	1.5643309E-10	37.499996	1
ATTAGCG	120	0.0	37.499996	1
ACCTCGC	30	1.140148E-4	37.499996	24
GCCCGTA	30	1.140148E-4	37.499996	9
GTCAACG	55	2.750312E-9	36.81818	1
TATTGCG	180	0.0	36.250004	1
TACGGGA	690	0.0	36.195652	4
TCGTTAG	50	4.881258E-8	36.0	1
TCACACG	25	0.0021073865	36.0	1
ACTCGAC	25	0.0021073865	36.0	12
TCGAGTA	25	0.0021073865	36.0	17
>>END_MODULE