FastQCFastQC Report
Sat 18 Jun 2016
SRR3552460_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552460_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1556775
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC39080.2510317804435452RNA PCR Primer, Index 40 (96% over 27bp)
CTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGCT28180.18101523983876927Illumina PCR Primer Index 8 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC21720.13951919834272775Illumina PCR Primer Index 8 (95% over 23bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA15900.10213421978127861No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG15740.10110645404763051No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCATA253.8914448E-545.042
TCGACAG453.8562575E-1045.01
GATTACG650.041.5384641
CGATGTA900.040.010
TAAGACG451.9290383E-840.01
CGTTCAT356.2497165E-638.57142617
TATACGG1300.038.0769272
CGTAAGG1850.037.70272
CGACATA301.14010756E-437.50000419
CGAAAGG2300.037.1739122
CACAACG5700.036.71052612
ACATACG4050.036.66666817
GTAATCG250.002107337436.01
TGATTCG504.8808943E-836.015
TTGACCG250.002107337436.01
CGAATTA250.002107337436.032
ATCGCGA250.002107337436.039
ATCGCCC250.002107337436.041
TCGCGAT250.002107337436.040
ACAACGA5850.035.7692313
Produced by FastQC (version 0.11.5)