##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552460_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1556775
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.826177193236017	31.0	31.0	34.0	30.0	34.0
2	32.00043936985114	33.0	31.0	34.0	30.0	34.0
3	32.056635673106264	33.0	31.0	34.0	30.0	34.0
4	35.65320935909171	37.0	35.0	37.0	33.0	37.0
5	35.60685070096835	37.0	35.0	37.0	33.0	37.0
6	35.61868478103772	37.0	35.0	37.0	33.0	37.0
7	35.91919448860625	37.0	35.0	37.0	35.0	37.0
8	35.91519647990236	37.0	35.0	37.0	35.0	37.0
9	37.64507844743139	39.0	37.0	39.0	35.0	39.0
10	37.09191244720657	39.0	37.0	39.0	33.0	39.0
11	36.926165309694724	39.0	37.0	39.0	33.0	39.0
12	36.7357839122545	39.0	35.0	39.0	33.0	39.0
13	36.708043872749755	39.0	35.0	39.0	33.0	39.0
14	37.847799457211224	40.0	37.0	41.0	33.0	41.0
15	37.84982800982801	40.0	37.0	41.0	33.0	41.0
16	37.85970162676045	40.0	37.0	41.0	33.0	41.0
17	37.811298999534294	40.0	36.0	41.0	33.0	41.0
18	37.794828411299	40.0	36.0	41.0	33.0	41.0
19	37.80422797128679	40.0	36.0	41.0	33.0	41.0
20	37.71461579226285	40.0	36.0	41.0	33.0	41.0
21	37.65347722053605	39.0	36.0	41.0	33.0	41.0
22	37.6295591848533	39.0	36.0	41.0	33.0	41.0
23	37.574208861267685	39.0	36.0	41.0	32.0	41.0
24	37.56982672512084	39.0	36.0	41.0	32.0	41.0
25	37.500780138427196	39.0	36.0	41.0	32.0	41.0
26	37.45673844967963	39.0	36.0	41.0	32.0	41.0
27	37.3930966260378	39.0	36.0	41.0	32.0	41.0
28	37.30666602431308	39.0	35.0	41.0	32.0	41.0
29	37.2615991392462	39.0	35.0	41.0	32.0	41.0
30	37.18414093237622	39.0	35.0	41.0	32.0	41.0
31	37.10288770053476	39.0	35.0	41.0	31.0	41.0
32	37.00641775465305	39.0	35.0	41.0	31.0	41.0
33	36.90446981741099	39.0	35.0	41.0	31.0	41.0
34	36.79341716047598	39.0	35.0	41.0	31.0	41.0
35	36.77127009362304	39.0	35.0	41.0	31.0	41.0
36	36.69506383388736	39.0	35.0	41.0	31.0	41.0
37	36.60589809060397	39.0	35.0	40.0	30.0	41.0
38	36.54266159207336	39.0	35.0	40.0	30.0	41.0
39	36.46760386054504	39.0	35.0	40.0	30.0	41.0
40	36.33004641004641	38.0	35.0	40.0	30.0	41.0
41	36.28853816383228	38.0	35.0	40.0	30.0	41.0
42	36.23766311766312	38.0	35.0	40.0	30.0	41.0
43	36.15601612307495	38.0	35.0	40.0	30.0	41.0
44	36.04101106454048	38.0	35.0	40.0	30.0	41.0
45	35.934650800533156	38.0	35.0	40.0	29.0	41.0
46	35.80137527902234	38.0	34.0	40.0	29.0	41.0
47	35.70678871384754	38.0	34.0	40.0	29.0	41.0
48	35.63801191565897	38.0	34.0	40.0	29.0	41.0
49	35.5631629490453	38.0	34.0	40.0	29.0	41.0
50	35.4527263091969	37.0	34.0	40.0	28.0	41.0
51	34.283241958536074	36.0	33.0	39.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	23.0
9	71.0
10	94.0
11	89.0
12	63.0
13	36.0
14	44.0
15	55.0
16	117.0
17	232.0
18	462.0
19	809.0
20	1257.0
21	2076.0
22	3083.0
23	4439.0
24	6416.0
25	8705.0
26	11589.0
27	14938.0
28	18749.0
29	23803.0
30	30109.0
31	38769.0
32	50039.0
33	67638.0
34	109293.0
35	126567.0
36	137780.0
37	196002.0
38	308201.0
39	395149.0
40	77.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.44301520772109	20.59269965152318	25.985001043824575	9.979284096931156
2	31.141558670970436	24.652567005508182	25.81702558173146	18.388848741789918
3	33.32877262289027	24.22032727915081	25.705577234989	16.74532286296992
4	29.34675852322911	27.135970194793725	24.52114146231793	18.99612981965923
5	26.00889659713189	30.423921247450657	23.45265051147404	20.11453164394341
6	26.48867048867049	36.30595301183536	22.018853077676606	15.186523421817538
7	83.73554302966068	6.092852210499269	6.045125339242986	4.126479420597068
8	84.78514878514879	4.977919095566154	6.146488734724029	4.0904433845610315
9	78.60827672592379	7.412888824653531	8.380530262883203	5.5983041865394805
10	41.152510799569626	27.213438036967446	15.685889097653805	15.948162065809123
11	31.49748679160444	26.549694079105844	22.049172166819226	19.903646962470493
12	27.784683078800725	23.772863772863772	27.81660805190217	20.62584509643333
13	24.627386745033807	24.923897159191277	28.80853045558928	21.640185640185642
14	21.217902394372985	27.332658862070623	28.492813669284256	22.956625074272132
15	21.45422427775369	26.37124825360119	30.82886094650801	21.345666522137112
16	24.498016733310852	25.351511939747233	28.457612692906807	21.692858634035105
17	23.915273562332388	26.039601098424626	26.080454786337135	23.964670552905847
18	24.601756837050957	25.83783783783784	27.264633617574795	22.295771707536414
19	24.85368791251144	26.682918212329977	25.792359204123912	22.671034671034672
20	26.9837323954971	25.933998169292288	26.03028697146344	21.05198246374717
21	25.692087809734872	26.966966966966964	26.233463409934	21.107481813364164
22	24.30582454111866	25.637487755134813	25.214626391096978	24.842061312649548
23	24.291371585489234	26.919497037144097	25.980119156589744	22.80901222077693
24	23.489714313243727	25.548521783815904	27.00666441842912	23.95509948451125
25	22.804901157842334	26.86920075155369	25.873488461723753	24.45240962888022
26	22.584509643333174	27.251850781262544	26.25652390358273	23.907115671821554
27	23.215429333076393	26.533314062725825	25.915434150728267	24.335822453469515
28	21.27719163013281	28.206516677104915	26.645019350901705	23.87127234186058
29	23.599556776027363	26.434905493729023	26.33264280323104	23.632894927012575
30	23.491384432560903	26.57256186667951	25.715983363042188	24.2200703377174
31	24.03654991890286	28.659697130285366	23.60013489425254	23.703618056559232
32	24.261052496346615	27.73894750365339	24.658027010968187	23.341972989031813
33	23.185945303592362	27.71235406529524	23.854763854763856	25.246936776348537
34	21.832377832377833	28.013104013104012	26.193252075605017	23.961266078913138
35	22.816784699137642	26.081032904562317	25.687784040725216	25.414398355574825
36	21.467007114065936	30.29936888760418	24.13849143260908	24.0951325657208
37	23.079121902651316	29.327230974289797	24.564564564564563	23.029082558494324
38	22.88089158677394	29.089046265516856	23.9904289316054	24.0396332161038
39	23.894075894075893	26.96529684764979	24.77711936535466	24.36350789291966
40	23.123893947423362	26.660757013698188	27.094474153297682	23.12087488558077
41	20.105859870565755	26.768865121806297	25.93380546321723	27.191469544410722
42	21.86719339660516	26.045767692826516	26.009410479998717	26.077628430569604
43	23.120682179505707	24.71879365997013	26.684652567005507	25.47587159351865
44	23.017199017199015	26.320373849785618	25.393971511618567	25.268455621396797
45	23.213566507684156	25.62303479950539	25.330571212924152	25.832827479886305
46	21.84169195933902	26.682340094104802	25.803214979685567	25.672752966870615
47	21.564130975895683	26.502095678566267	27.604117486470425	24.32965585906762
48	21.40765364294776	25.59631289043054	27.288529170882114	25.707504295739593
49	22.09587127234186	25.764738000032118	27.0575388222447	25.08185190538132
50	20.814825520707874	25.121485121485122	27.76457741163624	26.299111946170772
51	20.838207191148367	24.724446371505195	25.93444781680076	28.50289862054568
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	216.0
1	545.5
2	875.0
3	1662.0
4	2449.0
5	1930.0
6	1411.0
7	1387.5
8	1364.0
9	1459.0
10	1554.0
11	1655.5
12	1757.0
13	1924.0
14	2091.0
15	2117.5
16	2144.0
17	2407.0
18	2670.0
19	2913.0
20	3156.0
21	3579.5
22	4003.0
23	4236.5
24	4470.0
25	6550.0
26	11823.5
27	15017.0
28	17288.5
29	19560.0
30	23018.0
31	26476.0
32	29511.0
33	32546.0
34	34700.5
35	36855.0
36	39886.0
37	42917.0
38	46877.0
39	50837.0
40	57584.0
41	64331.0
42	69977.0
43	75623.0
44	83626.5
45	91630.0
46	104510.0
47	117390.0
48	131537.5
49	145685.0
50	143064.5
51	140444.0
52	129025.5
53	117607.0
54	107939.0
55	98271.0
56	91222.5
57	84174.0
58	81410.5
59	78647.0
60	75906.0
61	73165.0
62	68044.5
63	62924.0
64	56132.5
65	49341.0
66	40694.5
67	32048.0
68	27626.0
69	23204.0
70	20074.5
71	16945.0
72	13685.5
73	10426.0
74	8483.0
75	5139.5
76	3739.0
77	2816.5
78	1894.0
79	1453.5
80	1013.0
81	697.0
82	381.0
83	293.0
84	205.0
85	144.5
86	84.0
87	59.5
88	35.0
89	23.0
90	11.0
91	9.0
92	7.0
93	5.0
94	3.0
95	6.0
96	9.0
97	5.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1556775.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.614030285830676
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.81165059726791	16.48534431507714
2	11.521683371160512	5.44146760140672
3	4.476056324958692	3.1709318885597546
4	2.2319738471346398	2.108235920936776
5	1.3060822548306774	1.5420932960678817
6	0.8618749275988905	1.2211404385750995
7	0.6093067754128777	1.0071732053573068
8	0.4930590506156964	0.931448908315358
9	0.3960223657151062	0.8416515724076546
>10	6.942651631246897	43.43333219459233
>50	1.2652745732924557	18.549377858279062
>100	0.07699264461043527	3.3252654641564425
>500	0.004641400542206329	0.7663126046759579
>1k	0.0027302356130625466	1.1762247315924763
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC	3908	0.2510317804435452	RNA PCR Primer, Index 40 (96% over 27bp)
CTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGCT	2818	0.18101523983876927	Illumina PCR Primer Index 8 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC	2172	0.13951919834272775	Illumina PCR Primer Index 8 (95% over 23bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1590	0.10213421978127861	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1574	0.10110645404763051	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.423535835300542E-5	0.0	0.0	0.2076729135552665	0.0
2	6.423535835300542E-5	0.0	0.0	0.8249747073276485	0.0
3	6.423535835300542E-5	0.0	0.0	1.1331117213470154	0.0
4	6.423535835300542E-5	0.0	0.0	1.506447624094683	0.0
5	6.423535835300542E-5	0.0	0.0	2.502994973583209	0.0
6	6.423535835300542E-5	0.0	0.0	3.112460053636524	0.0
7	6.423535835300542E-5	0.0	0.0	3.726228902699491	0.0
8	6.423535835300542E-5	0.0	0.0	4.7570136981901685	0.0
9	6.423535835300542E-5	0.0	0.0	5.139278315748904	0.0
10	6.423535835300542E-5	0.0	0.0	6.099789629201394	0.0
11	6.423535835300542E-5	0.0	0.0	7.509563038974804	0.0
12	6.423535835300542E-5	0.0	0.0	8.525059819177466	0.0
13	6.423535835300542E-5	0.0	0.0	8.960832490244256	0.0
14	6.423535835300542E-5	0.0	0.0	9.125853125853126	0.0
15	6.423535835300542E-5	0.0	0.0	9.462253697547816	0.0
16	6.423535835300542E-5	0.0	0.0	10.154775095951566	0.0
17	6.423535835300542E-5	0.0	0.0	10.967352379117084	0.0
18	6.423535835300542E-5	0.0	0.0	11.868060573942927	0.0
19	6.423535835300542E-5	0.0	0.0	12.395240159946042	0.0
20	6.423535835300542E-5	0.0	0.0	12.933982110452698	0.0
21	6.423535835300542E-5	0.0	0.0	13.590660178895472	0.0
22	6.423535835300542E-5	0.0	0.0	14.26770085593615	0.0
23	1.2847071670601083E-4	0.0	0.0	14.90889820301585	0.0
24	1.2847071670601083E-4	0.0	0.0	15.449759920348155	0.0
25	1.9270607505901625E-4	0.0	0.0	15.944179473591237	0.0
26	2.5694143341202166E-4	0.0	0.0	16.35734129851777	0.0
27	2.5694143341202166E-4	0.0	0.0	16.80062950651186	0.0
28	2.5694143341202166E-4	0.0	0.0	17.248992307815836	0.0
29	2.5694143341202166E-4	0.0	0.0	17.709110179698413	0.0
30	2.5694143341202166E-4	0.0	0.0	18.259478730066967	0.0
31	2.5694143341202166E-4	0.0	0.0	18.76057876057876	0.0
32	2.5694143341202166E-4	0.0	0.0	19.22956111191405	0.0
33	2.5694143341202166E-4	0.0	0.0	19.696423696423697	0.0
34	3.211767917650271E-4	0.0	0.0	20.15153121035474	0.0
35	3.211767917650271E-4	0.0	0.0	20.63303945656887	0.0
36	3.854121501180325E-4	0.0	0.0	21.126623950153363	0.0
37	3.854121501180325E-4	0.0	0.0	21.597340656164185	0.0
38	4.4964750847103786E-4	0.0	0.0	22.076986076986078	0.0
39	4.4964750847103786E-4	0.0	0.0	22.53633312456842	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCATA	25	3.8914448E-5	45.0	42
TCGACAG	45	3.8562575E-10	45.0	1
GATTACG	65	0.0	41.538464	1
CGATGTA	90	0.0	40.0	10
TAAGACG	45	1.9290383E-8	40.0	1
CGTTCAT	35	6.2497165E-6	38.571426	17
TATACGG	130	0.0	38.076927	2
CGTAAGG	185	0.0	37.7027	2
CGACATA	30	1.14010756E-4	37.500004	19
CGAAAGG	230	0.0	37.173912	2
CACAACG	570	0.0	36.710526	12
ACATACG	405	0.0	36.666668	17
GTAATCG	25	0.0021073374	36.0	1
TGATTCG	50	4.8808943E-8	36.0	15
TTGACCG	25	0.0021073374	36.0	1
CGAATTA	25	0.0021073374	36.0	32
ATCGCGA	25	0.0021073374	36.0	39
ATCGCCC	25	0.0021073374	36.0	41
TCGCGAT	25	0.0021073374	36.0	40
ACAACGA	585	0.0	35.76923	13
>>END_MODULE