SRR3552457_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3552457_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2840661
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC	6379	0.22456041041152042	RNA PCR Primer, Index 23 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6358	0.22382114585302507	No Hit
CTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGCT	6216	0.21882230931462784	Illumina Paired End PCR Primer 2 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC	5088	0.17911324160116254	RNA PCR Primer, Index 1 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTG	3536	0.12447807042093371	Illumina Paired End PCR Primer 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC	3152	0.11096008992273276	RNA PCR Primer, Index 23 (95% over 23bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACGAG	140	0.0	41.785717	1
TAACGCG	55	6.184564E-11	40.90909	1
CGTTTTT	6090	0.0	40.899014	1
CCCGCAT	45	1.9299478E-8	40.0	35
CCCGATA	40	3.4606273E-7	39.375	24
TATAGCG	295	0.0	38.898304	1
TATGCGG	435	0.0	38.275864	2
CGTATGG	195	0.0	38.076923	2
GCGTTAG	255	0.0	37.941177	1
TAAGTCG	60	1.5643309E-10	37.500004	1
TATCGCG	85	0.0	37.058823	1
CTATGCG	110	0.0	36.81818	1
GTATTCG	55	2.752131E-9	36.81818	1
GTATGCG	220	0.0	36.81818	1
CTACGAA	295	0.0	36.61017	11
ACGACCA	695	0.0	36.25899	28
AACACGT	640	0.0	36.210938	41
CGACCAA	705	0.0	36.063828	29
CGAAACG	25	0.0021076298	36.0	10
ACGTTAC	25	0.0021076298	36.0	12