##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552457_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2840661
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.595950731185454	31.0	31.0	34.0	30.0	34.0
2	31.75542452971333	31.0	31.0	34.0	30.0	34.0
3	31.76852148144393	31.0	31.0	34.0	30.0	34.0
4	35.4279204734391	37.0	35.0	37.0	33.0	37.0
5	35.42496693551254	37.0	35.0	37.0	33.0	37.0
6	35.453993630355754	37.0	35.0	37.0	33.0	37.0
7	35.90854698959151	37.0	35.0	37.0	35.0	37.0
8	35.95853253872954	37.0	35.0	37.0	35.0	37.0
9	37.67102727146956	39.0	37.0	39.0	35.0	39.0
10	36.95113109237604	39.0	37.0	39.0	33.0	39.0
11	36.79104511238757	39.0	37.0	39.0	32.0	39.0
12	36.702298162293914	39.0	35.0	39.0	32.0	39.0
13	36.715327524122024	39.0	35.0	39.0	32.0	39.0
14	37.80777572543855	40.0	37.0	41.0	33.0	41.0
15	37.79417642583891	40.0	37.0	41.0	33.0	41.0
16	37.789298687875814	40.0	37.0	41.0	33.0	41.0
17	37.75284977686531	39.0	37.0	41.0	33.0	41.0
18	37.7215567081042	39.0	36.0	41.0	33.0	41.0
19	37.69345515005134	39.0	37.0	41.0	33.0	41.0
20	37.594756994938855	39.0	36.0	41.0	32.0	41.0
21	37.53340613329081	39.0	36.0	41.0	32.0	41.0
22	37.52871673177475	39.0	36.0	41.0	32.0	41.0
23	37.46131446166931	39.0	36.0	41.0	32.0	41.0
24	37.47188911313247	39.0	36.0	41.0	32.0	41.0
25	37.39485704207577	39.0	36.0	41.0	32.0	41.0
26	37.33859513683611	39.0	36.0	41.0	32.0	41.0
27	37.276538453550074	39.0	36.0	41.0	32.0	41.0
28	37.218351292181644	39.0	36.0	41.0	32.0	41.0
29	37.18117402956565	39.0	36.0	41.0	31.0	41.0
30	37.07463227748753	39.0	35.0	41.0	31.0	41.0
31	36.94903052493768	39.0	35.0	41.0	31.0	41.0
32	36.84509098410546	39.0	35.0	40.0	31.0	41.0
33	36.72322427772973	39.0	35.0	40.0	30.0	41.0
34	36.575618843642374	39.0	35.0	40.0	30.0	41.0
35	36.51436655060213	39.0	35.0	40.0	30.0	41.0
36	36.433202694724926	39.0	35.0	40.0	30.0	41.0
37	36.320029035495615	39.0	35.0	40.0	30.0	41.0
38	36.276047018634046	39.0	35.0	40.0	30.0	41.0
39	36.22945434178876	39.0	35.0	40.0	30.0	41.0
40	36.14591885480175	39.0	35.0	40.0	29.0	41.0
41	36.07703629542561	38.0	35.0	40.0	29.0	41.0
42	36.02516350947896	38.0	35.0	40.0	29.0	41.0
43	35.94573375703754	38.0	35.0	40.0	29.0	41.0
44	35.869042451739226	38.0	35.0	40.0	28.0	41.0
45	35.76145622444917	38.0	34.0	40.0	28.0	41.0
46	35.62432476103273	38.0	34.0	40.0	28.0	41.0
47	35.5021799503707	38.0	34.0	40.0	27.0	41.0
48	35.4235288899309	38.0	34.0	40.0	27.0	41.0
49	35.33622737806447	38.0	34.0	40.0	27.0	41.0
50	35.21171762487675	38.0	34.0	40.0	27.0	41.0
51	33.949713464577435	36.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	35.0
9	95.0
10	149.0
11	100.0
12	71.0
13	47.0
14	55.0
15	86.0
16	149.0
17	330.0
18	763.0
19	1360.0
20	2482.0
21	3861.0
22	6144.0
23	9465.0
24	14684.0
25	21170.0
26	28430.0
27	33756.0
28	39411.0
29	47745.0
30	59365.0
31	75006.0
32	95254.0
33	124264.0
34	182984.0
35	217074.0
36	258572.0
37	363042.0
38	576249.0
39	678358.0
40	102.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.74758586117808	20.78192364382797	26.66763123090013	9.802859264093815
2	32.54650942157477	23.900563988451985	26.09526444725365	17.4576621427196
3	31.415082616334722	24.66584361879154	27.52436844804783	16.39470531682591
4	27.753364445810323	26.66273800358438	27.125165586460337	18.458731964144963
5	24.799932128472914	30.346774923160492	25.738305274723032	19.11498767364356
6	24.60261889750308	35.75752263293649	24.731638164497628	14.9082203050628
7	83.28980473206764	5.388288148427426	7.391483883504579	3.9304232360003537
8	84.78065492503329	4.0749318556490906	7.005341362450499	4.139071856867116
9	78.38210895281064	7.295238678603325	9.314451812447878	5.008200556138166
10	35.482868247918354	33.32604629697102	16.87790271348816	14.31318274162246
11	26.800945272948795	26.88698158632797	27.34750820319637	18.964564937526866
12	23.78735090177955	24.81425977967804	31.04467586945433	20.353713449088083
13	23.755034479651037	25.19649475949436	32.32536370936201	18.723107051492594
14	21.47267836605635	28.45482090259978	29.48081450056871	20.59168623077516
15	20.665049437437272	27.463819160399638	32.00149542659261	19.869635975570475
16	23.800516851535612	27.60332894350998	29.27793918387305	19.31821502108136
17	23.56173439914161	26.679248245390774	28.43714895934432	21.321868396123296
18	24.385028695785945	25.480372349956575	29.94077786824968	20.193821086007798
19	22.820920905380827	28.063292311190953	28.3422414712632	20.77354531216502
20	24.270794719961305	27.866507126334326	28.239554103780772	19.623144049923592
21	23.22712917873692	28.69684203782148	28.99205501818063	19.08397376526097
22	22.68897978322651	25.804663069616545	27.917410771647866	23.58894637550908
23	20.957692593378795	28.222093378970598	28.5452223971815	22.274991630469103
24	21.185949326582794	26.931971115173546	29.446843533952133	22.43523602429153
25	20.87563422738581	29.051865041270325	27.663490997341817	22.40900973400205
26	21.80566424504719	28.39018805834276	28.3650178602797	21.43912983633035
27	21.764547054365163	27.47360561503115	28.353999298050702	22.407848032552987
28	19.612371909214087	28.160699217541268	29.220910203646266	23.00601866959838
29	22.6201225700638	26.323626789680286	27.761777980547485	23.294472659708426
30	23.662415191393833	26.127686478604804	27.5448214341662	22.66507689583516
31	22.63737207642869	28.951536279760237	25.535289145730516	22.875802498080553
32	23.387831212524127	28.281199340576013	26.156130562569768	22.174838884330093
33	22.692570496796343	27.931668016704563	25.495826499536552	23.879934986962542
34	22.30160515457494	28.57144868747098	26.841464011369187	22.285482146584897
35	23.568880623207065	26.79196848902421	26.683085380480104	22.95606550728862
36	22.954551775097414	30.09771317309598	25.08842132165718	21.859313730149427
37	21.90050132697988	30.319562946793017	26.30148405599964	21.478451670227457
38	22.269535153965926	29.80250019273683	26.711423855222428	21.216540798074814
39	24.222073665249038	27.91550980564031	26.09167373368382	21.77074279542684
40	23.94822895093783	26.526818934043874	28.221037286744178	21.303914828274124
41	21.07872076252675	28.333546311932327	27.086794235567	23.500938689973918
42	22.00621615884472	28.180800172917504	27.16314970353731	22.64983396470047
43	21.912294356841596	27.04951417997431	27.86235316357707	23.17583829960703
44	22.79673639339576	27.294386764207346	25.593691045851653	24.31518579654524
45	24.23802065786801	25.229480040032936	26.457574487064804	24.074924815034247
46	23.60478775890541	26.690161198397135	26.31616373794691	23.38888730475055
47	21.23435355362713	27.736150142519644	28.493544284235256	22.53595201961797
48	21.452718222976976	26.939610182277995	28.290598561391167	23.317073033353857
49	23.123948968215498	26.008876103132334	28.223360689642305	22.643814239009863
50	21.884659943583554	25.3833174743484	28.2053367156447	24.526685866423342
51	21.77232693376647	24.693302016678512	26.65140261368745	26.882968435867564
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	481.0
1	721.0
2	961.0
3	6302.0
4	11643.0
5	8548.0
6	5453.0
7	5487.5
8	5522.0
9	5792.5
10	6063.0
11	6357.0
12	6651.0
13	6889.5
14	7128.0
15	7322.5
16	7517.0
17	7617.0
18	7717.0
19	7975.5
20	8234.0
21	8919.5
22	9605.0
23	11128.0
24	12651.0
25	15105.0
26	20528.0
27	23497.0
28	29178.5
29	34860.0
30	40143.5
31	45427.0
32	52590.5
33	59754.0
34	67493.5
35	75233.0
36	81861.5
37	88490.0
38	98203.5
39	107917.0
40	119513.5
41	131110.0
42	145023.5
43	158937.0
44	180549.5
45	202162.0
46	233031.0
47	263900.0
48	294502.0
49	325104.0
50	319864.5
51	314625.0
52	276085.5
53	237546.0
54	207502.0
55	177458.0
56	154429.5
57	131401.0
58	115395.0
59	99389.0
60	89229.5
61	79070.0
62	69848.5
63	60627.0
64	51191.5
65	41756.0
66	34672.5
67	27589.0
68	22410.0
69	17231.0
70	14068.5
71	10906.0
72	9109.5
73	7313.0
74	6267.0
75	4333.5
76	3446.0
77	2631.0
78	1816.0
79	1338.0
80	860.0
81	640.5
82	421.0
83	344.0
84	267.0
85	169.5
86	72.0
87	54.5
88	37.0
89	25.5
90	14.0
91	12.0
92	10.0
93	9.5
94	9.0
95	5.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2840661.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.654668604801387
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.7824696436966	19.422842048551363
2	12.931637095784932	7.411035509945468
3	5.651857464126646	4.858563079083665
4	2.855000315170783	3.272363515912892
5	1.702718710732988	2.4395420191624226
6	1.1413394536375026	1.9622822285740626
7	0.7924194229434386	1.5894561172316524
8	0.5921443587726151	1.357416029346552
9	0.47302033059884663	1.2198816734979188
>10	5.212193312322018	35.159868654286626
>50	0.767998173525884	14.101489365158626
>100	0.090290511580904	4.5826308265688445
>500	0.004319504441819844	0.7853988749597729
>1k	0.0020980450145980153	0.9870443513440756
>5k	4.936576504936507E-4	0.8501857063760854
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC	6379	0.22456041041152042	RNA PCR Primer, Index 23 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6358	0.22382114585302507	No Hit
CTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGCT	6216	0.21882230931462784	Illumina Paired End PCR Primer 2 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC	5088	0.17911324160116254	RNA PCR Primer, Index 1 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTG	3536	0.12447807042093371	Illumina Paired End PCR Primer 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC	3152	0.11096008992273276	RNA PCR Primer, Index 23 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.056092226421949E-4	0.0	0.0	0.24448535041668118	0.0
2	1.056092226421949E-4	0.0	0.0	0.8988048908335067	0.0
3	1.056092226421949E-4	0.0	0.0	1.3039570719631803	0.0
4	1.056092226421949E-4	0.0	0.0	1.7301254883986508	0.0
5	1.056092226421949E-4	0.0	0.0	2.9215735351736796	0.0
6	1.056092226421949E-4	0.0	0.0	3.8418170982035518	0.0
7	1.056092226421949E-4	0.0	0.0	4.723302076523739	0.0
8	1.056092226421949E-4	0.0	0.0	6.270301172860823	0.0
9	1.056092226421949E-4	0.0	0.0	6.868964652945213	0.0
10	1.056092226421949E-4	0.0	0.0	8.261915096521548	0.0
11	1.056092226421949E-4	0.0	0.0	10.22170544109276	0.0
12	1.056092226421949E-4	0.0	0.0	11.697629530591648	0.0
13	1.056092226421949E-4	0.0	0.0	12.355751003023592	0.0
14	1.056092226421949E-4	0.0	0.0	12.653111370909798	0.0
15	1.4081229685625988E-4	0.0	0.0	13.119059261207163	0.0
16	1.4081229685625988E-4	0.0	0.0	14.072675338591969	0.0
17	1.4081229685625988E-4	0.0	0.0	15.324003814605122	0.0
18	1.4081229685625988E-4	0.0	0.0	16.62067385020599	0.0
19	1.4081229685625988E-4	0.0	0.0	17.43266796002761	0.0
20	1.7601537107032482E-4	0.0	0.0	18.22522997288307	0.0
21	2.112184452843898E-4	0.0	0.0	19.206515666600133	0.0
22	2.112184452843898E-4	0.0	0.0	20.22916497251872	0.0
23	2.4642151949845476E-4	0.0	0.0	21.33626645347685	0.0
24	2.4642151949845476E-4	0.0	0.0	22.177549521044575	0.0
25	2.8162459371251975E-4	0.0	0.0	22.954481368948986	0.0
26	3.168276679265847E-4	0.0	0.0	23.65794440096865	0.0
27	3.168276679265847E-4	0.0	0.0	24.3643996942965	0.0
28	3.168276679265847E-4	0.0	0.0	25.110669664560465	0.0
29	3.168276679265847E-4	0.0	0.0	25.87010558458049	0.0
30	3.168276679265847E-4	0.0	0.0	26.779013757713432	0.0
31	3.168276679265847E-4	0.0	0.0	27.576187373290935	0.0
32	3.168276679265847E-4	0.0	0.0	28.367130044732548	0.0
33	3.168276679265847E-4	0.0	0.0	29.136211607087223	0.0
34	3.168276679265847E-4	0.0	0.0	29.887128383147445	0.0
35	3.168276679265847E-4	0.0	0.0	30.67110084589467	0.0
36	3.5203074214064964E-4	0.0	0.0	31.40297275880508	0.0
37	3.5203074214064964E-4	0.0	0.0	32.13766091765262	0.0
38	3.5203074214064964E-4	0.0	0.0	32.90473590477709	0.0
39	3.5203074214064964E-4	0.0	0.0	33.645302977018375	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACGAG	140	0.0	41.785717	1
TAACGCG	55	6.184564E-11	40.90909	1
CGTTTTT	6090	0.0	40.899014	1
CCCGCAT	45	1.9299478E-8	40.0	35
CCCGATA	40	3.4606273E-7	39.375	24
TATAGCG	295	0.0	38.898304	1
TATGCGG	435	0.0	38.275864	2
CGTATGG	195	0.0	38.076923	2
GCGTTAG	255	0.0	37.941177	1
TAAGTCG	60	1.5643309E-10	37.500004	1
TATCGCG	85	0.0	37.058823	1
CTATGCG	110	0.0	36.81818	1
GTATTCG	55	2.752131E-9	36.81818	1
GTATGCG	220	0.0	36.81818	1
CTACGAA	295	0.0	36.61017	11
ACGACCA	695	0.0	36.25899	28
AACACGT	640	0.0	36.210938	41
CGACCAA	705	0.0	36.063828	29
CGAAACG	25	0.0021076298	36.0	10
ACGTTAC	25	0.0021076298	36.0	12
>>END_MODULE