FastQCFastQC Report
Sat 18 Jun 2016
SRR3552456_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552456_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2124249
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGC77150.3631871781509607TruSeq Adapter, Index 20 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGCT51680.24328598012756508TruSeq Adapter, Index 22 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGC43930.20680249820054053TruSeq Adapter, Index 20 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGC33210.15633760448986914TruSeq Adapter, Index 20 (95% over 23bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA26850.1263976115794335No Hit
ACTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGC22560.10620223900305473TruSeq Adapter, Index 22 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG253.8919272E-545.045
ATCGTGT253.8919272E-545.043
CATACCG253.8919272E-545.039
TAGTCGA253.8919272E-545.024
TGCGTAG950.045.01
CTAACGG1850.040.1351362
GTGCGAT451.929584E-840.012
TTAATCG750.039.00000420
GGACGTA356.2507934E-638.5714268
CGAACGC301.1402478E-437.50000411
ATTAGCG1450.037.241381
ACGCATT850.037.05882617
AGGGCGC3300.036.8181846
GTTCACG552.752131E-936.818181
AACACGT4850.036.64948341
GACACGA5300.036.50943425
AAGGGCG8700.036.2068985
ACACGAC5350.036.16822426
ATAGGGA13400.036.1007464
GTATGCG1000.036.01