FastQCFastQC Report
Sat 18 Jun 2016
SRR3552455_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552455_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2123245
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGC38780.18264496089711738No Hit
CCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGC33190.15631733502257159No Hit
CTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGCT31310.14746296352987998No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC25080.11812108353016257No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21820.10276722658006966No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACA356.2507897E-638.57142640
GCTACGA2650.038.20754610
CTACGCG659.094947E-1238.0769271
CGTACCG301.14024755E-437.50000444
CTACGAA2650.037.3584911
CGACCAA5100.036.6176529
TATTACG504.8823495E-836.01
TACGTTA250.0021075136.045
ACGGGTA1450.035.6896555
AACACGT4950.035.45454441
CGAATAT2800.035.35714314
ACGTATG702.1827873E-1135.3571431
GACACGA5250.035.14285725
CACGACC5250.035.14285727
ATACCGG653.6743586E-1034.6153872
CGTGTAC4750.034.57894518
TAAGGGA13600.034.577214
GCGATTA1500.034.59
TACGAAT2900.034.13792812
AGGGTAC4750.034.1052636
Produced by FastQC (version 0.11.5)