##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552455_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2123245
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.817523177965803	31.0	31.0	34.0	30.0	34.0
2	31.997046030957332	33.0	31.0	34.0	30.0	34.0
3	32.04019507875916	33.0	31.0	34.0	30.0	34.0
4	35.64327291480729	37.0	35.0	37.0	33.0	37.0
5	35.60053973987929	37.0	35.0	37.0	33.0	37.0
6	35.606572015947286	37.0	35.0	37.0	33.0	37.0
7	35.91046582000664	37.0	35.0	37.0	35.0	37.0
8	35.902594848922284	37.0	35.0	37.0	35.0	37.0
9	37.61874536381812	39.0	37.0	39.0	35.0	39.0
10	37.07480436784262	39.0	37.0	39.0	33.0	39.0
11	36.905202649717765	39.0	37.0	39.0	33.0	39.0
12	36.73449554808795	39.0	35.0	39.0	33.0	39.0
13	36.70677053284006	39.0	35.0	39.0	33.0	39.0
14	37.822573937534294	40.0	37.0	41.0	33.0	41.0
15	37.83580980998425	40.0	37.0	41.0	33.0	41.0
16	37.851421762443806	40.0	37.0	41.0	33.0	41.0
17	37.81466434631896	40.0	36.0	41.0	33.0	41.0
18	37.795221465257185	40.0	36.0	41.0	33.0	41.0
19	37.78772633398407	40.0	36.0	41.0	33.0	41.0
20	37.67945149994466	40.0	36.0	41.0	33.0	41.0
21	37.62058123297123	39.0	36.0	41.0	32.0	41.0
22	37.594730707007436	39.0	36.0	41.0	32.0	41.0
23	37.547183674046096	39.0	36.0	41.0	32.0	41.0
24	37.54108028041983	39.0	36.0	41.0	32.0	41.0
25	37.47251023786704	39.0	36.0	41.0	32.0	41.0
26	37.40308442972902	39.0	36.0	41.0	32.0	41.0
27	37.324219767384356	39.0	35.0	41.0	32.0	41.0
28	37.206941733054826	39.0	35.0	41.0	32.0	41.0
29	37.15940129377439	39.0	35.0	41.0	31.0	41.0
30	37.092044959484184	39.0	35.0	41.0	31.0	41.0
31	36.97764647979861	39.0	35.0	41.0	31.0	41.0
32	36.8673629279711	39.0	35.0	41.0	31.0	41.0
33	36.783871385544295	39.0	35.0	41.0	31.0	41.0
34	36.66113566733938	39.0	35.0	41.0	30.0	41.0
35	36.60498388080509	39.0	35.0	41.0	30.0	41.0
36	36.49928717599712	39.0	35.0	40.0	30.0	41.0
37	36.355344767089996	38.0	35.0	40.0	30.0	41.0
38	36.277096142932166	38.0	35.0	40.0	30.0	41.0
39	36.26416828957563	38.0	35.0	40.0	30.0	41.0
40	36.1659219732061	38.0	35.0	40.0	30.0	41.0
41	36.08903918294874	38.0	35.0	40.0	30.0	41.0
42	36.00930933547471	38.0	35.0	40.0	29.0	41.0
43	35.915031002074656	38.0	35.0	40.0	29.0	41.0
44	35.81032146549268	38.0	34.0	40.0	29.0	41.0
45	35.73605259873448	38.0	34.0	40.0	29.0	41.0
46	35.600224656127764	38.0	34.0	40.0	28.0	41.0
47	35.467825898565636	38.0	34.0	40.0	28.0	41.0
48	35.35599848345339	37.0	34.0	40.0	28.0	41.0
49	35.27345266325836	37.0	34.0	40.0	28.0	41.0
50	35.160724268749014	37.0	34.0	40.0	27.0	41.0
51	33.92968639982668	36.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	38.0
9	100.0
10	133.0
11	119.0
12	70.0
13	39.0
14	58.0
15	80.0
16	155.0
17	353.0
18	683.0
19	1180.0
20	1948.0
21	3078.0
22	4768.0
23	6855.0
24	9793.0
25	13357.0
26	17517.0
27	22414.0
28	27629.0
29	34432.0
30	42921.0
31	54669.0
32	69439.0
33	94151.0
34	150107.0
35	171775.0
36	190690.0
37	270214.0
38	415860.0
39	518523.0
40	94.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.027111802924296	21.032099451547044	25.23147352283886	10.7093152226898
2	31.41135384753055	24.985953104799492	25.774086363090458	17.8286066845795
3	32.65798341689254	24.67600300483458	25.885425374838984	16.780588203433894
4	29.75478571714522	26.748538204493595	24.77952379494594	18.717152283415246
5	26.236821469025006	30.315154398102905	23.320059625714414	20.127964507157674
6	26.694046141636974	36.25398858822228	22.15048192742712	14.901483342713629
7	83.51155895810423	6.0807396226059645	6.329462685653328	4.078238733636486
8	84.8359468643515	4.992829372022541	5.901108915834018	4.270114847791941
9	78.27269109311455	7.462680943555737	8.521767389067206	5.742860574262509
10	41.73785879632355	26.539047542794165	15.637243935579736	16.085849725302545
11	31.363596758734865	26.615675534382515	21.751517135328235	20.26921057155439
12	27.440074037616952	23.708851310140847	27.962858737451402	20.888215914790802
13	25.405311209963994	23.804412585452926	29.08491483554653	21.705361369036545
14	21.58356666329133	26.559629246742606	28.97183320813189	22.884970881834175
15	22.527923061163456	25.14702730961335	30.53684336946513	21.78820625975806
16	24.604885446568815	25.281350009066312	28.023567699441188	22.09019684492369
17	24.439289860567197	24.9933003492296	26.652694342857274	23.914715447345923
18	25.167043840913315	24.437170463135434	27.52353119870764	22.872254497243606
19	25.43559504437783	25.701461677762104	26.332853721544147	22.53008955631592
20	26.675960616885945	25.743143160586747	26.392667826840523	21.18822839568679
21	25.96026365304051	26.24153124109559	26.09034755762995	21.707857548233953
22	24.35244166358569	25.006676101910045	25.6771121561572	24.963770078347057
23	24.221886781789195	25.832628829927774	26.4435804629235	23.50190392535953
24	23.597936177878672	24.856669861462056	27.289455526799784	24.255938433859495
25	24.020167244006227	26.501651952553757	25.456600627812616	24.0215801756274
26	23.801398331327757	26.71321491396424	25.770930815803165	23.714455938904834
27	24.542057087147267	25.919053147422932	25.977407223377426	23.561482542052378
28	22.253767228934958	26.814098231716077	26.88276670850514	24.049367830843828
29	24.831613874046564	26.05384682408295	25.593937581390747	23.520601720479736
30	26.001709647261624	25.479678510958465	25.40055434017271	23.118057501607208
31	26.021208103633825	26.712131666388007	24.389130788015514	22.877529441962658
32	25.820524715706384	27.39650864596408	24.216988618835792	22.565978019493745
33	25.354021792115372	27.26388146445653	23.816469601953614	23.565627141474486
34	24.444941587051893	26.43726936834892	25.994503695993632	23.123285348605556
35	26.001709647261624	26.705961864975546	24.372411097164953	22.91991739059788
36	25.899884375095667	28.79272057628771	24.198055335112056	21.109339713504564
37	25.281114520462783	28.192931103099266	25.312293211570026	21.213661164867926
38	23.887351671615853	29.322758325110854	24.740197198156597	22.049692805116695
39	25.6146605785013	26.45398905919948	25.20533428784714	22.726016074452076
40	26.088604941963833	25.61164632437613	26.582424543564215	21.717324190095823
41	23.13129196112554	27.065270376240143	25.72684734922254	24.076590313411785
42	23.742620375886908	27.166718866640448	25.74733485772956	23.343325899743082
43	24.483326229427128	25.075391676419816	27.228652369368582	23.21262972478447
44	24.04404578840407	25.463665285918484	26.162077386264894	24.33021153941255
45	25.237407835647797	24.46321550268575	25.489992911793035	24.809383749873426
46	24.719803885090982	26.248172019715106	25.423632223318553	23.608391871875362
47	23.622709578969925	25.851467918210098	27.92122435234747	22.60459815047251
48	23.137603055700122	26.451775466326303	27.29765053020259	23.112970947770982
49	24.273882665448408	25.724351170025127	27.283709604873675	22.718056559652798
50	22.730725846522656	25.01294009876392	27.660350077358004	24.595983977355417
51	22.89759307098333	24.879465158283665	26.131793551851057	26.091148218881948
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	362.0
1	745.0
2	1128.0
3	2846.5
4	4565.0
5	3456.0
6	2347.0
7	2302.5
8	2258.0
9	2384.5
10	2511.0
11	2645.5
12	2780.0
13	2933.0
14	3086.0
15	3206.0
16	3326.0
17	3309.5
18	3293.0
19	3686.0
20	4079.0
21	4945.0
22	5811.0
23	6822.5
24	7834.0
25	9793.5
26	14370.0
27	16987.0
28	20985.0
29	24983.0
30	29381.5
31	33780.0
32	36727.0
33	39674.0
34	45419.0
35	51164.0
36	54397.5
37	57631.0
38	63629.0
39	69627.0
40	76421.5
41	83216.0
42	89158.5
43	95101.0
44	107356.5
45	119612.0
46	135453.0
47	151294.0
48	171839.0
49	192384.0
50	192842.5
51	193301.0
52	175992.5
53	158684.0
54	147196.0
55	135708.0
56	129521.0
57	123334.0
58	115484.0
59	107634.0
60	104826.0
61	102018.0
62	96461.0
63	90904.0
64	79842.5
65	68781.0
66	58845.0
67	48909.0
68	42027.5
69	35146.0
70	30054.0
71	24962.0
72	20654.5
73	16347.0
74	14208.5
75	9277.5
76	6485.0
77	5271.0
78	4057.0
79	3279.5
80	2502.0
81	1686.0
82	870.0
83	697.0
84	524.0
85	371.0
86	218.0
87	172.0
88	126.0
89	84.5
90	43.0
91	31.0
92	19.0
93	15.5
94	12.0
95	8.0
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2123245.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.276952756269843
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.46413507755398	16.40190343211641
2	11.06723503305056	5.152230140137048
3	4.326596311088666	3.021299337859867
4	2.166481624620308	2.0171636169445457
5	1.257816531215563	1.4639067986579937
6	0.8344780853315933	1.1654464181043607
7	0.5878005995202833	0.9577544749598507
8	0.49270884796066217	0.9175008461261178
9	0.38224561791523887	0.800776187055377
>10	6.977503752113027	43.209905393099156
>50	1.3597950803082763	19.756210851449836
>100	0.07750738094710255	3.2979642085845304
>500	0.002644598531119944	0.4386258160896518
>1k	0.003051459843599936	1.3993124788152111
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGC	3878	0.18264496089711738	No Hit
CCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGC	3319	0.15631733502257159	No Hit
CTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGCT	3131	0.14746296352987998	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	2508	0.11812108353016257	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2182	0.10276722658006966	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4129316211741933E-4	0.0	0.0	0.16917501277525673	0.0
2	1.4129316211741933E-4	0.0	0.0	0.6961043120318192	0.0
3	1.4129316211741933E-4	0.0	0.0	0.9641845382892695	0.0
4	1.4129316211741933E-4	0.0	0.0	1.283789670998872	0.0
5	1.4129316211741933E-4	0.0	0.0	2.1539671587593516	0.0
6	1.4129316211741933E-4	0.0	0.0	2.7959561897001994	0.0
7	1.4129316211741933E-4	0.0	0.0	3.4083678520378005	0.0
8	1.4129316211741933E-4	0.0	0.0	4.449839750005299	0.0
9	1.4129316211741933E-4	0.0	0.0	4.867313946341567	0.0
10	1.4129316211741933E-4	0.0	0.0	5.786284672753262	0.0
11	1.4129316211741933E-4	0.0	0.0	7.066306526095669	0.0
12	1.4129316211741933E-4	0.0	0.0	8.0220605723786	0.0
13	1.4129316211741933E-4	0.0	0.0	8.436614710031108	0.0
14	1.4129316211741933E-4	0.0	0.0	8.631881860077382	0.0
15	1.4129316211741933E-4	0.0	0.0	8.938158337827241	0.0
16	1.4129316211741933E-4	0.0	0.0	9.572517538013749	0.0
17	1.4129316211741933E-4	0.0	0.0	10.298057925486695	0.0
18	1.4129316211741933E-4	0.0	0.0	11.07507612169109	0.0
19	1.4129316211741933E-4	0.0	0.0	11.548266921622329	0.0
20	1.8839088282322577E-4	0.0	0.0	12.03261988136084	0.0
21	2.8258632423483867E-4	0.0	0.0	12.632833234035639	0.0
22	3.296840449406451E-4	0.0	0.0	13.24670492571512	0.0
23	3.7678176564645154E-4	0.0	0.0	13.847436353317681	0.0
24	4.709772070580644E-4	0.0	0.0	14.371775277935424	0.0
25	5.180749277638708E-4	0.0	0.0	14.842611191831372	0.0
26	5.180749277638708E-4	0.0	0.0	15.251749091602711	0.0
27	5.180749277638708E-4	0.0	0.0	15.660180525563465	0.0
28	5.180749277638708E-4	0.0	0.0	16.08500196632984	0.0
29	5.180749277638708E-4	0.0	0.0	16.529792840675476	0.0
30	5.180749277638708E-4	0.0	0.0	17.04353477813441	0.0
31	5.180749277638708E-4	0.0	0.0	17.499016835080266	0.0
32	5.180749277638708E-4	0.0	0.0	17.964577804257164	0.0
33	6.122703691754837E-4	0.0	0.0	18.42349799481454	0.0
34	6.122703691754837E-4	0.0	0.0	18.861789383702774	0.0
35	6.122703691754837E-4	0.0	0.0	19.337617655993537	0.0
36	6.593680898812901E-4	0.0	0.0	19.77680390157518	0.0
37	6.593680898812901E-4	0.0	0.0	20.209961638906485	0.0
38	7.064658105870967E-4	0.0	0.0	20.674203871903618	0.0
39	7.064658105870967E-4	0.0	0.0	21.158180049876485	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGACA	35	6.2507897E-6	38.571426	40
GCTACGA	265	0.0	38.207546	10
CTACGCG	65	9.094947E-12	38.076927	1
CGTACCG	30	1.14024755E-4	37.500004	44
CTACGAA	265	0.0	37.35849	11
CGACCAA	510	0.0	36.61765	29
TATTACG	50	4.8823495E-8	36.0	1
TACGTTA	25	0.00210751	36.0	45
ACGGGTA	145	0.0	35.689655	5
AACACGT	495	0.0	35.454544	41
CGAATAT	280	0.0	35.357143	14
ACGTATG	70	2.1827873E-11	35.357143	1
GACACGA	525	0.0	35.142857	25
CACGACC	525	0.0	35.142857	27
ATACCGG	65	3.6743586E-10	34.615387	2
CGTGTAC	475	0.0	34.578945	18
TAAGGGA	1360	0.0	34.57721	4
GCGATTA	150	0.0	34.5	9
TACGAAT	290	0.0	34.137928	12
AGGGTAC	475	0.0	34.105263	6
>>END_MODULE