FastQCFastQC Report
Sat 18 Jun 2016
SRR3552454_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552454_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1241117
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCGGTTGCAATCGTATGCCGTCTTCTGC40730.3281721223704131No Hit
CCTGTCTCTTATACACATCTGACGCGGTTGCAATCGTATGCCGTCTTCTGC27920.22495864612280708No Hit
CTGTCTCTTATACACATCTGACGCGGTTGCAATCGTATGCCGTCTTCTGCT26100.21029443638271011No Hit
TCTGTCTCTTATACACATCTGACGCGGTTGCAATCGTATGCCGTCTTCTGC17170.1383431215590472No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAAG1250.045.0000041
CGTAGAA207.033504E-445.038
TAGATCG207.033504E-445.01
CGTAAGT207.033504E-445.05
TATAGCG700.045.01
ACGTAAC207.033504E-445.038
TACAGCG351.212029E-745.01
TAGCACG253.8909875E-544.9999961
CCGAACT253.8909875E-544.99999617
ATACGAG253.8909875E-544.9999961
AATTGCG1200.043.1250041
ACTTGCG501.0822987E-940.4999961
CGCTACA501.0822987E-940.49999628
ACGTCTT501.0822987E-940.49999628
GTCGATG451.9284926E-840.0000041
ACGTTAC403.4585173E-739.37515
AGTACGG1150.039.1304322
TGTCGAC356.2486943E-638.57142612
GTTAACG950.037.8947371
GGACCGT301.13997434E-437.5000048