##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552454_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1241117
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.718315033957314	31.0	31.0	34.0	30.0	34.0
2	31.885375029106847	33.0	31.0	34.0	30.0	34.0
3	31.93540173891744	33.0	31.0	34.0	30.0	34.0
4	35.54373761700146	37.0	35.0	37.0	33.0	37.0
5	35.52714369394666	37.0	35.0	37.0	33.0	37.0
6	35.53575126277378	37.0	35.0	37.0	33.0	37.0
7	35.90045579909066	37.0	35.0	37.0	35.0	37.0
8	35.903186403860396	37.0	35.0	37.0	35.0	37.0
9	37.61423379101245	39.0	37.0	39.0	35.0	39.0
10	37.0445147395451	39.0	37.0	39.0	33.0	39.0
11	36.86614557692788	39.0	37.0	39.0	33.0	39.0
12	36.66441036582369	39.0	35.0	39.0	32.0	39.0
13	36.60550455758804	39.0	35.0	39.0	32.0	39.0
14	37.68514491381554	40.0	36.0	41.0	33.0	41.0
15	37.70858025472216	40.0	36.0	41.0	33.0	41.0
16	37.73485819628609	40.0	36.0	41.0	33.0	41.0
17	37.71433555418224	39.0	36.0	41.0	33.0	41.0
18	37.67783375781655	39.0	36.0	41.0	33.0	41.0
19	37.67348444989473	39.0	36.0	41.0	33.0	41.0
20	37.57735894359678	39.0	36.0	41.0	32.0	41.0
21	37.50855318233494	39.0	36.0	41.0	32.0	41.0
22	37.49306954944618	39.0	36.0	41.0	32.0	41.0
23	37.439947241073966	39.0	36.0	41.0	32.0	41.0
24	37.430036813612254	39.0	36.0	41.0	32.0	41.0
25	37.3690216152063	39.0	35.0	41.0	32.0	41.0
26	37.302876360568746	39.0	35.0	41.0	32.0	41.0
27	37.22438899797521	39.0	35.0	41.0	32.0	41.0
28	37.123873091739135	39.0	35.0	41.0	31.0	41.0
29	37.07208909393715	39.0	35.0	41.0	31.0	41.0
30	36.995425894577224	39.0	35.0	41.0	31.0	41.0
31	36.859400846173244	39.0	35.0	41.0	31.0	41.0
32	36.76598338432235	39.0	35.0	41.0	31.0	41.0
33	36.68035003952085	39.0	35.0	40.0	30.0	41.0
34	36.55069103074086	39.0	35.0	40.0	30.0	41.0
35	36.52683671241309	39.0	35.0	40.0	30.0	41.0
36	36.4156288246797	39.0	35.0	40.0	30.0	41.0
37	36.27104052236816	38.0	35.0	40.0	30.0	41.0
38	36.207872424598165	38.0	35.0	40.0	30.0	41.0
39	36.21213471413251	38.0	35.0	40.0	30.0	41.0
40	36.13128899209341	38.0	35.0	40.0	30.0	41.0
41	36.05234961732052	38.0	35.0	40.0	30.0	41.0
42	35.9821684821012	38.0	35.0	40.0	29.0	41.0
43	35.88987823065835	38.0	35.0	40.0	29.0	41.0
44	35.78576153577785	38.0	34.0	40.0	29.0	41.0
45	35.722409732523204	38.0	34.0	40.0	28.0	41.0
46	35.60392372354903	38.0	34.0	40.0	28.0	41.0
47	35.4747175326742	38.0	34.0	40.0	28.0	41.0
48	35.376766251691016	38.0	34.0	40.0	28.0	41.0
49	35.316930635870754	37.0	34.0	40.0	28.0	41.0
50	35.214157085915346	37.0	34.0	40.0	27.0	41.0
51	34.02296479703364	36.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	18.0
9	57.0
10	95.0
11	82.0
12	40.0
13	26.0
14	32.0
15	54.0
16	115.0
17	222.0
18	395.0
19	789.0
20	1271.0
21	2084.0
22	2934.0
23	4381.0
24	6148.0
25	8395.0
26	10535.0
27	13132.0
28	16375.0
29	20272.0
30	25655.0
31	32669.0
32	42024.0
33	55978.0
34	88512.0
35	104692.0
36	111424.0
37	154721.0
38	240463.0
39	297472.0
40	51.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.50347308110356	21.490560519274172	24.777518960742622	10.228447438879654
2	30.621287114752278	24.579954992156257	26.061362466230015	18.737395426861447
3	32.16948925846636	24.916426090368596	26.27125403970778	16.64283061145726
4	28.965037139931205	25.89643039294442	25.98699397397667	19.151538493147704
5	25.298662414582996	29.926832039203394	24.401970160750356	20.372535385463255
6	25.99706554660036	35.75359937862426	23.138672663415292	15.11066241136009
7	82.99249788698407	6.082504711481674	6.4164780596833335	4.5085193418509295
8	83.68791983350482	5.107254191184231	6.419056382274999	4.78576959303595
9	77.04462995833592	7.802084734960523	9.12653682126665	6.026748485436909
10	40.68407732711743	28.02950890206161	15.884320334021693	15.40209343679927
11	31.22123055280042	26.089562869576355	22.781413839307657	19.907792738315568
12	28.64540571114569	22.75982038760246	28.136186999291766	20.45858690196009
13	25.388581414967327	24.13253544992132	29.180488221497246	21.298394913614107
14	20.738254330574797	28.32786916946589	28.018148168142087	22.915728331817224
15	20.622632676854803	26.233143208899723	32.292443017056414	20.851781097189065
16	22.5969026288416	25.07330090555524	30.37078696045578	21.959009505147378
17	22.476930055748166	24.886453090240483	27.669752327943296	24.96686452606805
18	23.147052211838208	25.26723910799707	28.88148337344505	22.70422530671967
19	24.759631847762943	26.98899459116264	26.083761643745113	22.167611917329307
20	26.382847064378296	26.20220333779974	26.191970620014065	21.222978977807895
21	24.599695274498696	27.316763850628107	26.860400751903324	21.223140122969873
22	22.61454802407831	25.98965286914932	25.28133930967024	26.114459797102125
23	22.40795992642112	27.30500025380363	26.37712641112804	23.90991340864721
24	23.530980560253383	24.815549218969686	27.53672699672956	24.116743224047372
25	22.37589203918728	27.035082107488655	25.03696267152895	25.55206318179511
26	22.465408176666664	27.628257448733677	26.35762784652857	23.548706528071083
27	24.3268765152681	26.77692755799816	26.257798418682526	22.638397508051213
28	21.19502029220452	27.402412504219985	27.96037762757258	23.442189576002907
29	24.20013584537155	25.896188675201454	26.768225719251287	23.135449760175714
30	25.987799699786564	25.161850172062746	26.12300049068702	22.72734963746367
31	24.598244968040888	27.227570003472678	24.181120716258018	23.99306431222842
32	25.710630021182528	27.077866147994108	25.186183091521592	22.025320739301772
33	25.81311834420123	26.996326696032686	24.0394741188784	23.151080840887683
34	23.189433389438708	25.513791205825076	27.231356914779187	24.06541848995703
35	24.649086266645288	25.7240050696268	25.46754254433708	24.15936611939084
36	25.417265253799602	28.02402996655432	24.86598765466914	21.692717124976937
37	23.861408714891503	27.81341323984765	26.89488581656685	21.430292228693993
38	22.740966403650905	28.223849967408395	26.445935395293112	22.589248233647595
39	24.452005733544862	25.33822355184886	26.303644217265575	23.9061264973407
40	24.82537907385041	24.680670718393188	27.216209269553154	23.277740938203248
41	21.2660853086373	27.910180909616095	25.72505251317966	25.098681268566942
42	23.37466975313367	26.93049889736423	26.51498609720115	23.17984525230095
43	24.18369903884968	24.623061322985667	28.061335071552477	23.13190456661217
44	22.99823465475052	24.103851611089045	27.679259892500063	25.218653841660377
45	24.133582893474184	23.441142132450043	26.777813856389045	25.647461117686728
46	24.344763628247783	25.434266068388396	26.264969378390596	23.95600092497323
47	22.231344828892038	26.15829128116044	28.078899894208202	23.531463995739323
48	22.49062739451639	25.19585180124033	28.061979652200396	24.251541152042876
49	22.763043290842038	24.31616036199649	28.98123222871011	23.93956411845136
50	21.340856663795595	23.971873723428168	28.857069881405216	25.830199731371017
51	21.43456257548644	23.518411237619016	26.527394274673537	28.519631912221005
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	287.0
1	538.5
2	790.0
3	1400.0
4	2010.0
5	1571.0
6	1132.0
7	1231.0
8	1330.0
9	1477.0
10	1624.0
11	1799.0
12	1974.0
13	2161.0
14	2348.0
15	2419.5
16	2491.0
17	2600.0
18	2709.0
19	2924.5
20	3140.0
21	3577.0
22	4014.0
23	4110.0
24	4206.0
25	5607.0
26	8470.0
27	9932.0
28	11937.0
29	13942.0
30	15871.0
31	17800.0
32	19577.0
33	21354.0
34	25673.0
35	29992.0
36	32455.0
37	34918.0
38	36287.5
39	37657.0
40	43322.0
41	48987.0
42	56815.5
43	64644.0
44	71767.0
45	78890.0
46	89953.0
47	101016.0
48	113564.5
49	126113.0
50	123925.5
51	121738.0
52	107700.0
53	93662.0
54	83794.0
55	73926.0
56	67764.0
57	61602.0
58	58704.5
59	55807.0
60	56099.5
61	56392.0
62	51939.5
63	47487.0
64	40525.5
65	33564.0
66	28138.5
67	22713.0
68	19585.5
69	16458.0
70	14955.5
71	13453.0
72	11144.5
73	8836.0
74	7520.0
75	5165.5
76	4127.0
77	3255.0
78	2383.0
79	1810.5
80	1238.0
81	961.0
82	684.0
83	445.0
84	206.0
85	201.0
86	196.0
87	131.0
88	66.0
89	62.5
90	59.0
91	30.5
92	2.0
93	2.5
94	3.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1241117.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.554793829520726
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.14608395790206	16.978678357050157
2	12.056654043630184	5.920973086303931
3	5.115388787290997	3.7682195108931746
4	2.515266959717938	2.470474464883176
5	1.5351716233557895	1.884791135221603
6	0.9978928978982728	1.470183262310104
7	0.7279568938365705	1.2512382011454708
8	0.501517841277392	0.9851733755514128
9	0.3916595461959586	0.865540746738487
>10	5.432353782423567	35.01590270421118
>50	1.4808075235705387	23.5358237710453
>100	0.09234492104341076	3.8667912998430145
>500	0.004600814571505588	0.8014560234933318
>1k	0.002300407285752794	1.1847540613098166
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGGTTGCAATCGTATGCCGTCTTCTGC	4073	0.3281721223704131	No Hit
CCTGTCTCTTATACACATCTGACGCGGTTGCAATCGTATGCCGTCTTCTGC	2792	0.22495864612280708	No Hit
CTGTCTCTTATACACATCTGACGCGGTTGCAATCGTATGCCGTCTTCTGCT	2610	0.21029443638271011	No Hit
TCTGTCTCTTATACACATCTGACGCGGTTGCAATCGTATGCCGTCTTCTGC	1717	0.1383431215590472	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.05725809895441E-5	0.0	0.0	0.23486907358452103	0.0
2	8.05725809895441E-5	0.0	0.0	1.1087592869971163	0.0
3	8.05725809895441E-5	0.0	0.0	1.549410732428933	0.0
4	8.05725809895441E-5	0.0	0.0	2.0337325167570826	0.0
5	8.05725809895441E-5	0.0	0.0	3.37204308699341	0.0
6	8.05725809895441E-5	0.0	0.0	4.133373404763612	0.0
7	8.05725809895441E-5	0.0	0.0	4.931444819465046	0.0
8	8.05725809895441E-5	0.0	0.0	6.339611817419309	0.0
9	8.05725809895441E-5	0.0	0.0	6.840048117945367	0.0
10	8.05725809895441E-5	0.0	0.0	8.134527204123382	0.0
11	8.05725809895441E-5	0.0	0.0	9.924608235968083	0.0
12	8.05725809895441E-5	0.0	0.0	11.25429754003853	0.0
13	8.05725809895441E-5	0.0	0.0	11.788252034256239	0.0
14	8.05725809895441E-5	0.0	0.0	11.992906389969681	0.0
15	8.05725809895441E-5	0.0	0.0	12.38537543196975	0.0
16	1.611451619790882E-4	0.0	0.0	13.24605174210006	0.0
17	1.611451619790882E-4	0.0	0.0	14.267631496466489	0.0
18	1.611451619790882E-4	0.0	0.0	15.433677888547171	0.0
19	1.611451619790882E-4	0.0	0.0	16.0738270445091	8.05725809895441E-5
20	1.611451619790882E-4	0.0	0.0	16.680860869684324	8.05725809895441E-5
21	8.862983908849851E-4	0.0	0.0	17.4254320906087	8.05725809895441E-5
22	0.0010474435528640732	0.0	0.0	18.24558039250127	8.05725809895441E-5
23	0.0016114516197908819	0.0	0.0	19.034869395874846	8.05725809895441E-5
24	0.0019337419437490584	0.0	0.0	19.669136753424535	8.05725809895441E-5
25	0.004189774211456293	0.0	0.0	20.217755457382342	8.05725809895441E-5
26	0.004189774211456293	0.0	0.0	20.714324274020903	8.05725809895441E-5
27	0.004270346792445837	0.0	0.0	21.23039165525893	0.0044314919544249255
28	0.004270346792445837	0.0	0.0	21.756691754282635	0.0044314919544249255
29	0.004270346792445837	0.0	0.0	22.28452273234514	0.0044314919544249255
30	0.004270346792445837	0.0	0.0	22.893248581721142	0.0044314919544249255
31	0.004270346792445837	0.0	0.0	23.46233272125029	0.0044314919544249255
32	0.004270346792445837	0.0	0.0	24.011837723598983	0.0044314919544249255
33	0.004270346792445837	0.0	0.0	24.519364411252123	0.0044314919544249255
34	0.004270346792445837	0.0	0.0	25.050257147392227	0.0044314919544249255
35	0.004753782278383102	0.0	0.0	25.604677077181282	0.0044314919544249255
36	0.004753782278383102	0.0	0.0	26.156760402121638	0.0044314919544249255
37	0.004753782278383102	0.0	0.0	26.66162819460212	0.0044314919544249255
38	0.004753782278383102	0.0	0.0	27.199450172707326	0.0044314919544249255
39	0.00491492744036219	0.0	0.0	27.745893416978415	0.0044314919544249255
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTAAG	125	0.0	45.000004	1
CGTAGAA	20	7.033504E-4	45.0	38
TAGATCG	20	7.033504E-4	45.0	1
CGTAAGT	20	7.033504E-4	45.0	5
TATAGCG	70	0.0	45.0	1
ACGTAAC	20	7.033504E-4	45.0	38
TACAGCG	35	1.212029E-7	45.0	1
TAGCACG	25	3.8909875E-5	44.999996	1
CCGAACT	25	3.8909875E-5	44.999996	17
ATACGAG	25	3.8909875E-5	44.999996	1
AATTGCG	120	0.0	43.125004	1
ACTTGCG	50	1.0822987E-9	40.499996	1
CGCTACA	50	1.0822987E-9	40.499996	28
ACGTCTT	50	1.0822987E-9	40.499996	28
GTCGATG	45	1.9284926E-8	40.000004	1
ACGTTAC	40	3.4585173E-7	39.375	15
AGTACGG	115	0.0	39.130432	2
TGTCGAC	35	6.2486943E-6	38.571426	12
GTTAACG	95	0.0	37.894737	1
GGACCGT	30	1.13997434E-4	37.500004	8
>>END_MODULE