SRR3552452_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3552452_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	719912
Sequences flagged as poor quality	0
Sequence length	51
%GC	51

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC	2706	0.3758792741335052	TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC	2316	0.32170598628721286	TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGCT	1597	0.22183266843725344	Illumina Paired End PCR Primer 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC	1142	0.15863049928324574	TruSeq Adapter, Index 14 (95% over 21bp)
GCGAGCGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC	923	0.1282101145695585	No Hit
ACTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC	767	0.10654079943104157	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTTTA	25	3.889347E-5	45.000004	35
TAAACGG	30	2.1643664E-6	45.000004	2
TCGATGC	25	3.889347E-5	45.000004	24
TCGCTAG	25	3.889347E-5	45.000004	1
CGAACAC	20	7.031528E-4	45.0	18
AACGGTA	20	7.031528E-4	45.0	35
TCGGAAT	20	7.031528E-4	45.0	30
CGTAGAC	20	7.031528E-4	45.0	36
GTATACG	20	7.031528E-4	45.0	1
TCGAAAC	20	7.031528E-4	45.0	11
ACGGTAT	20	7.031528E-4	45.0	36
TAGGGCA	260	0.0	43.26923	5
CTACGAA	100	0.0	42.750004	11
GCGTTAG	85	0.0	42.35294	1
CTAACGG	70	0.0	41.785717	2
GTATGCG	60	3.6379788E-12	41.250004	1
TGGTCAA	465	0.0	41.129032	14
CGTTAGG	115	0.0	41.08696	2
CACGACC	455	0.0	41.043957	27
GTGAAAC	440	0.0	40.909092	37