FastQCFastQC Report
Sat 18 Jun 2016
SRR3552450_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552450_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2037265
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGC56680.2782161378122139Illumina PCR Primer Index 8 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGCT40590.19923770348972766RNA PCR Primer, Index 40 (96% over 26bp)
CCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGC34820.17091541846544264RNA PCR Primer, Index 40 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGC24010.11785408378389656Illumina PCR Primer Index 8 (95% over 23bp)
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG22710.11147297970563476No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGA207.034572E-445.028
CGTAAGG2650.040.754722
TACGAAT2100.039.6428612
GTACGAG1150.039.1304361
TATTGCG1100.038.8636361
GTTAACG1100.038.8636361
GCTACGA2200.038.86363610
AGCCGAT356.250666E-638.57142619
TCTATCG356.250666E-638.5714261
GCGCATT659.094947E-1238.076921
CGAATAT2200.037.84090814
TAGTACG1250.037.81
CGTTAGG3100.037.741942
CGCACGG3400.037.720592
TCGCATA301.1402315E-437.5000041
CGACACG301.1402315E-437.5000042
TCGTAAG1150.037.1739161
TAACGGG6200.037.0161323
ACAACGG1300.036.3461532
TTAACGG2050.036.2195132
Produced by FastQC (version 0.11.5)