##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552450_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2037265
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.754964621686426	31.0	31.0	34.0	30.0	34.0
2	31.917336232645237	33.0	31.0	34.0	30.0	34.0
3	31.966429502298425	33.0	31.0	34.0	30.0	34.0
4	35.58274794884318	37.0	35.0	37.0	33.0	37.0
5	35.55447622179736	37.0	35.0	37.0	33.0	37.0
6	35.567669399906244	37.0	35.0	37.0	33.0	37.0
7	35.92306204642008	37.0	35.0	37.0	35.0	37.0
8	35.9300954956768	37.0	35.0	37.0	35.0	37.0
9	37.6443329660108	39.0	37.0	39.0	35.0	39.0
10	37.071461493718296	39.0	37.0	39.0	33.0	39.0
11	36.89342574480983	39.0	37.0	39.0	33.0	39.0
12	36.69780416391584	39.0	35.0	39.0	32.0	39.0
13	36.648248509644056	39.0	35.0	39.0	32.0	39.0
14	37.747800605223176	40.0	36.0	41.0	33.0	41.0
15	37.75265564371842	40.0	36.0	41.0	33.0	41.0
16	37.77112894002499	40.0	36.0	41.0	33.0	41.0
17	37.7220881917669	40.0	36.0	41.0	33.0	41.0
18	37.701555762259694	39.0	36.0	41.0	33.0	41.0
19	37.7093269653187	39.0	36.0	41.0	33.0	41.0
20	37.62374457912937	39.0	36.0	41.0	33.0	41.0
21	37.56282663276501	39.0	36.0	41.0	32.0	41.0
22	37.537259512140054	39.0	36.0	41.0	32.0	41.0
23	37.49154528252338	39.0	36.0	41.0	32.0	41.0
24	37.48832037069306	39.0	36.0	41.0	32.0	41.0
25	37.43103621767418	39.0	36.0	41.0	32.0	41.0
26	37.35980493455687	39.0	35.0	41.0	32.0	41.0
27	37.299881949574555	39.0	35.0	41.0	32.0	41.0
28	37.22066348756789	39.0	35.0	41.0	32.0	41.0
29	37.14767151057914	39.0	35.0	41.0	31.0	41.0
30	37.066943181176725	39.0	35.0	41.0	31.0	41.0
31	36.98941423918832	39.0	35.0	41.0	31.0	41.0
32	36.89481878891553	39.0	35.0	41.0	31.0	41.0
33	36.817337950634794	39.0	35.0	41.0	31.0	41.0
34	36.70403997516278	39.0	35.0	41.0	30.0	41.0
35	36.67492152469119	39.0	35.0	41.0	30.0	41.0
36	36.59798602538207	39.0	35.0	40.0	30.0	41.0
37	36.50501284810763	39.0	35.0	40.0	30.0	41.0
38	36.435743018213145	39.0	35.0	40.0	30.0	41.0
39	36.3581644999546	38.0	35.0	40.0	30.0	41.0
40	36.25102723504306	38.0	35.0	40.0	30.0	41.0
41	36.20190205986948	38.0	35.0	40.0	30.0	41.0
42	36.14670109190508	38.0	35.0	40.0	30.0	41.0
43	36.06080603161592	38.0	35.0	40.0	30.0	41.0
44	35.93013181888463	38.0	35.0	40.0	29.0	41.0
45	35.8114624263412	38.0	35.0	40.0	29.0	41.0
46	35.66427931565113	38.0	34.0	40.0	29.0	41.0
47	35.56519058639892	38.0	34.0	40.0	28.0	41.0
48	35.49943281801828	38.0	34.0	40.0	28.0	41.0
49	35.41840212245339	37.0	34.0	40.0	28.0	41.0
50	35.300677133313535	37.0	34.0	40.0	28.0	41.0
51	34.001860337265896	36.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	36.0
9	102.0
10	141.0
11	134.0
12	80.0
13	45.0
14	58.0
15	80.0
16	160.0
17	300.0
18	567.0
19	1043.0
20	1767.0
21	2786.0
22	4190.0
23	6354.0
24	9105.0
25	12574.0
26	16097.0
27	20442.0
28	26101.0
29	32463.0
30	40980.0
31	52610.0
32	68153.0
33	90234.0
34	146556.0
35	169689.0
36	181486.0
37	255485.0
38	397462.0
39	499888.0
40	92.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.69883152167244	20.757731566585594	25.494965063455172	10.048471848286797
2	31.70260128162021	24.226008889368835	25.87881301647061	18.19257681254034
3	33.213597641936616	24.544475068290083	25.42359486861061	16.818332421162687
4	29.80608806414482	26.470930389517317	24.765064927733995	18.95791661860386
5	26.052918986975186	30.24716961220067	23.545537767546197	20.15437363327795
6	25.73234213516651	36.11670548504981	22.71447258947658	15.436479790307104
7	82.68556127946044	6.404370565439449	6.662461682697146	4.247606472402952
8	83.88236189204645	5.143857082902813	6.439172125373969	4.534608899676773
9	77.3806549467055	7.965041366734322	8.701764375277639	5.952539311282528
10	40.87936522740046	27.682260285235355	15.745177971447013	15.693196515917174
11	31.643404269940334	26.353174476565393	22.11725033316726	19.886170920327007
12	28.41024608973305	23.332359805916266	27.633911150488522	20.623482953862162
13	25.59892797451485	24.694578270377196	28.79723550937163	20.909258245736318
14	21.097746243124973	27.601809288433266	28.66676647367917	22.633677994762586
15	21.62389281708565	25.833703519178897	31.39591560253575	21.146488061199697
16	24.406741391031602	25.379270737974686	28.942381084444097	21.271606786549615
17	23.516381030450138	25.348739609231004	27.080178572743357	24.0547007875755
18	24.21167594790074	25.378092687991007	28.120740306243906	22.289491057864343
19	24.8924415822193	26.74723219610605	25.90473011611155	22.455596105563096
20	26.873970740183527	26.341688489224524	26.184418816403365	20.59992195418858
21	25.644626496798406	27.459903350815924	26.030585122701268	20.864885029684405
22	23.74364650646823	26.05792569940582	25.35708412994873	24.841343664177217
23	23.480303249699965	26.778401435257564	26.546963698880603	23.194331616161865
24	24.020979106792687	25.025315803295108	27.158617067489992	23.795088022422217
25	22.87532549766476	27.047782198192184	25.534282481660465	24.542609822482593
26	22.04376946543528	27.71058257025964	26.441724567005277	23.803923397299812
27	22.99784269596739	26.504308472388228	26.568561281914725	23.92928754972966
28	20.487810864075122	28.866740458408703	26.494295047526954	24.151153629989224
29	22.967311567223707	28.074403673552535	25.337793561465986	23.62049119775778
30	23.56875516930787	27.186743010850336	25.629017334514653	23.615484485327144
31	23.950688791099832	28.332985644970094	23.554863996583656	24.161461567346418
32	24.496273189791214	28.640407605294353	24.028980029598507	22.834339175315925
33	23.3986742029142	27.527984822789374	23.529045067774685	25.544295906521736
34	21.355002908310897	28.23402944634105	26.06479765764395	24.3461699877041
35	22.3737216316974	26.773394722826925	25.78957573020692	25.063307915268755
36	20.79945417017423	31.261470648148375	23.83789050516256	24.101184676514837
37	22.470174474111122	28.93919053240496	24.776452744242896	23.814182249241018
38	22.55528858543194	29.175684066628545	25.174584553310442	23.094442794629074
39	23.347478114040147	26.527967642893778	25.02595391370293	25.09860032936314
40	22.556810233327525	25.18346901360402	27.6894267559694	24.570293997099053
41	19.187979963333195	26.859441457051485	26.7742291748987	27.178349404716617
42	21.51659209773888	26.438926698294036	26.08713152191787	25.95734968204922
43	22.35997771522114	25.316834088839695	26.35602143069262	25.967166765246546
44	21.96076602700189	26.009969247986888	25.2962672995413	26.73299742546993
45	22.865999268627302	25.42256407487489	25.57144014156234	26.139996514935465
46	21.707583451342856	26.99614434057425	26.020620783255982	25.27565142482691
47	20.52595023229673	26.70344800504598	27.989584074727635	24.78101768792965
48	20.634478087043167	25.0594792528218	28.10061528568939	26.205427374445637
49	22.004402961814	25.250323350177812	27.824018966604736	24.92125472140345
50	20.86787924005959	24.61152574652782	27.839039103896646	26.681555909515943
51	20.299421037518435	24.170395113055985	26.526642336662142	29.003541512763437
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	321.0
1	841.5
2	1362.0
3	2476.0
4	3590.0
5	2894.5
6	2199.0
7	2139.5
8	2080.0
9	2191.0
10	2302.0
11	2469.5
12	2637.0
13	2779.0
14	2921.0
15	3335.0
16	3749.0
17	3542.5
18	3336.0
19	3821.5
20	4307.0
21	5052.5
22	5798.0
23	6342.5
24	6887.0
25	9760.5
26	14379.0
27	16124.0
28	20777.0
29	25430.0
30	30491.5
31	35553.0
32	38539.5
33	41526.0
34	44164.0
35	46802.0
36	51012.0
37	55222.0
38	60010.0
39	64798.0
40	72076.5
41	79355.0
42	89047.0
43	98739.0
44	110115.0
45	121491.0
46	141524.0
47	161557.0
48	181082.5
49	200608.0
50	200658.5
51	200709.0
52	179028.0
53	157347.0
54	141386.5
55	125426.0
56	117167.0
57	108908.0
58	103909.5
59	98911.0
60	95265.0
61	91619.0
62	84468.0
63	77317.0
64	66777.5
65	56238.0
66	47475.0
67	38712.0
68	33470.5
69	28229.0
70	23536.5
71	18844.0
72	15992.0
73	13140.0
74	11144.5
75	7249.5
76	5350.0
77	4187.0
78	3024.0
79	2415.5
80	1807.0
81	1219.5
82	632.0
83	488.5
84	345.0
85	237.5
86	130.0
87	89.5
88	49.0
89	33.0
90	17.0
91	18.0
92	19.0
93	13.5
94	8.0
95	7.0
96	6.0
97	3.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2037265.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.19517308877446
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.0624496011748	14.884626766552882
2	12.659988253897044	5.61981261194193
3	5.402310961390595	3.5971568060234347
4	2.956820225688668	2.6250854680619664
5	1.7739174446666852	1.9686202364786787
6	1.0905489399913026	1.4522953490931887
7	0.8003841291626217	1.2435264998990683
8	0.5865431578001348	1.0414741529128275
9	0.4397245553043618	0.878378635472822
>10	5.133840179499534	29.613357351341467
>50	1.905780066615295	28.44684127208473
>100	0.17900921397891978	6.238470193804914
>500	0.0055661992500104845	0.871520016519677
>1k	0.0028944236100054517	1.2387385038613485
>5k	2.2264797000041934E-4	0.28009613595109606
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGC	5668	0.2782161378122139	Illumina PCR Primer Index 8 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGCT	4059	0.19923770348972766	RNA PCR Primer, Index 40 (96% over 26bp)
CCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGC	3482	0.17091541846544264	RNA PCR Primer, Index 40 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGC	2401	0.11785408378389656	Illumina PCR Primer Index 8 (95% over 23bp)
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2271	0.11147297970563476	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.817083197325826E-5	0.0	0.0	0.22849261141775862	0.0
2	9.817083197325826E-5	0.0	0.0	0.9603561637783989	0.0
3	9.817083197325826E-5	0.0	0.0	1.311365973498784	0.0
4	9.817083197325826E-5	0.0	0.0	1.7324697572480752	0.0
5	9.817083197325826E-5	0.0	0.0	2.851960839655126	0.0
6	9.817083197325826E-5	0.0	0.0	3.5255600032396375	0.0
7	9.817083197325826E-5	0.0	0.0	4.203282341767026	0.0
8	9.817083197325826E-5	0.0	0.0	5.36537956525047	0.0
9	9.817083197325826E-5	0.0	0.0	5.790704694774612	0.0
10	9.817083197325826E-5	0.0	0.0	6.91795127290755	0.0
11	9.817083197325826E-5	0.0	0.0	8.595641705914547	0.0
12	9.817083197325826E-5	0.0	0.0	9.844276517782419	0.0
13	9.817083197325826E-5	0.0	0.0	10.350838010764432	0.0
14	9.817083197325826E-5	0.0	0.0	10.549830287174226	0.0
15	9.817083197325826E-5	0.0	0.0	10.937654158884582	0.0
16	9.817083197325826E-5	0.0	0.0	11.796501682402633	0.0
17	9.817083197325826E-5	0.0	0.0	12.77231975221682	0.0
18	1.472562479598874E-4	0.0	0.0	13.812243375309546	0.0
19	1.472562479598874E-4	0.0	0.0	14.434793706267962	0.0
20	1.9634166394651652E-4	0.0	0.0	15.059160197617885	0.0
21	0.0012271353996657283	0.0	0.0	15.825432626585153	0.0
22	0.0012762208156523575	0.0	0.0	16.634114854964867	0.0
23	0.001472562479598874	0.0	0.0	17.427433348140767	0.0
24	0.0015216478955855032	0.0	0.0	18.07045229756561	0.0
25	0.0019634166394651652	0.0	0.0	18.671748643401816	0.0
26	0.0019634166394651652	0.0	0.0	19.186065632109717	0.0
27	0.0020125020554517944	0.0	0.0	19.694590541731195	0.0
28	0.0020615874714384236	0.0	0.0	20.22093345735582	0.0
29	0.0020615874714384236	0.0	0.0	20.765879745639374	0.0
30	0.0020615874714384236	0.0	0.0	21.425784078163616	0.0
31	0.0020615874714384236	0.0	0.0	21.99345691404898	0.0
32	0.0020615874714384236	0.0	0.0	22.56834530608438	0.0
33	0.002208843719398311	0.0	0.0	23.13425106699423	0.0
34	0.002208843719398311	0.0	0.0	23.702807440367355	0.0
35	0.002208843719398311	0.0	0.0	24.28986901556744	0.0
36	0.002208843719398311	0.0	0.0	24.85356593275789	0.0
37	0.002208843719398311	0.0	0.0	25.416133885380646	0.0
38	0.002208843719398311	0.0	0.0	25.980076229651026	0.0
39	0.002208843719398311	0.0	0.0	26.576218606808638	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACGA	20	7.034572E-4	45.0	28
CGTAAGG	265	0.0	40.75472	2
TACGAAT	210	0.0	39.64286	12
GTACGAG	115	0.0	39.130436	1
TATTGCG	110	0.0	38.863636	1
GTTAACG	110	0.0	38.863636	1
GCTACGA	220	0.0	38.863636	10
AGCCGAT	35	6.250666E-6	38.571426	19
TCTATCG	35	6.250666E-6	38.571426	1
GCGCATT	65	9.094947E-12	38.07692	1
CGAATAT	220	0.0	37.840908	14
TAGTACG	125	0.0	37.8	1
CGTTAGG	310	0.0	37.74194	2
CGCACGG	340	0.0	37.72059	2
TCGCATA	30	1.1402315E-4	37.500004	1
CGACACG	30	1.1402315E-4	37.500004	2
TCGTAAG	115	0.0	37.173916	1
TAACGGG	620	0.0	37.016132	3
ACAACGG	130	0.0	36.346153	2
TTAACGG	205	0.0	36.219513	2
>>END_MODULE