##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552442_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1222786
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.568367645687797	31.0	31.0	34.0	30.0	34.0
2	31.714815184341333	31.0	31.0	34.0	30.0	34.0
3	31.78152677573999	31.0	31.0	34.0	30.0	34.0
4	35.41293652364355	37.0	35.0	37.0	33.0	37.0
5	35.40576928424107	37.0	35.0	37.0	33.0	37.0
6	35.41772885852471	37.0	35.0	37.0	33.0	37.0
7	35.84723655651929	37.0	35.0	37.0	35.0	37.0
8	35.86883886469096	37.0	35.0	37.0	35.0	37.0
9	37.58822721228408	39.0	37.0	39.0	35.0	39.0
10	36.95098079304147	39.0	37.0	39.0	33.0	39.0
11	36.749967696718805	39.0	37.0	39.0	32.0	39.0
12	36.540700498697234	39.0	35.0	39.0	32.0	39.0
13	36.452330170610395	39.0	35.0	39.0	32.0	39.0
14	37.45442702157205	39.0	36.0	41.0	32.0	41.0
15	37.46148222174608	39.0	36.0	41.0	32.0	41.0
16	37.49464092653989	39.0	36.0	41.0	32.0	41.0
17	37.44781588928888	39.0	36.0	41.0	32.0	41.0
18	37.44452831484822	39.0	36.0	41.0	32.0	41.0
19	37.41109400990852	39.0	36.0	41.0	32.0	41.0
20	37.323085151449234	39.0	35.0	41.0	32.0	41.0
21	37.26761755532039	39.0	35.0	41.0	32.0	41.0
22	37.24006326536287	39.0	35.0	41.0	32.0	41.0
23	37.201074431666704	39.0	35.0	41.0	32.0	41.0
24	37.18226574396501	39.0	35.0	41.0	32.0	41.0
25	37.1295500602722	39.0	35.0	41.0	32.0	41.0
26	37.07396388247821	39.0	35.0	41.0	31.0	41.0
27	37.002850048986495	39.0	35.0	40.0	31.0	41.0
28	36.946934295943855	39.0	35.0	40.0	31.0	41.0
29	36.89162208268659	39.0	35.0	40.0	31.0	41.0
30	36.76384584056409	39.0	35.0	40.0	31.0	41.0
31	36.6801721642217	39.0	35.0	40.0	31.0	41.0
32	36.54987544836136	39.0	35.0	40.0	30.0	41.0
33	36.389728047262565	38.0	35.0	40.0	30.0	41.0
34	36.29839154193784	38.0	35.0	40.0	30.0	41.0
35	36.259018340085674	38.0	35.0	40.0	30.0	41.0
36	36.087056933919754	38.0	35.0	40.0	30.0	41.0
37	36.00909398700999	38.0	35.0	40.0	30.0	41.0
38	35.94365489954906	38.0	35.0	40.0	29.0	41.0
39	35.85153739084353	38.0	35.0	40.0	29.0	41.0
40	35.77184805845013	38.0	34.0	40.0	29.0	41.0
41	35.68524091705335	38.0	34.0	40.0	29.0	41.0
42	35.5772817156886	38.0	34.0	40.0	28.0	41.0
43	35.51157684173682	38.0	34.0	40.0	28.0	41.0
44	35.450193247223964	38.0	34.0	40.0	28.0	41.0
45	35.42834805108989	38.0	34.0	40.0	28.0	41.0
46	35.2546553526128	38.0	34.0	40.0	27.0	41.0
47	35.11496124424061	37.0	34.0	40.0	27.0	41.0
48	35.01603960136933	37.0	34.0	40.0	26.0	41.0
49	34.93663159375394	37.0	34.0	40.0	26.0	41.0
50	34.827760540274426	37.0	34.0	40.0	26.0	41.0
51	33.57870469567038	36.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	19.0
9	48.0
10	64.0
11	40.0
12	29.0
13	24.0
14	19.0
15	40.0
16	103.0
17	244.0
18	479.0
19	762.0
20	1473.0
21	2184.0
22	3223.0
23	4825.0
24	6872.0
25	9074.0
26	11334.0
27	14218.0
28	17924.0
29	22000.0
30	28270.0
31	35837.0
32	45626.0
33	60457.0
34	95360.0
35	107206.0
36	112067.0
37	155331.0
38	232907.0
39	254684.0
40	42.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.61335507603129	20.02149190455239	24.66253293707975	11.702620082336566
2	30.39697870273294	24.690665414880446	25.396267212742053	19.516088669644567
3	32.13677618160496	25.001758279862546	25.449342730453246	17.412122808079257
4	29.704952461019346	26.3133532768612	24.887265637650415	19.09442862446904
5	25.21700444722135	29.49289573155074	24.38407047512811	20.906029346099807
6	25.616501988082952	34.81320525423091	23.497079619819004	16.073213137867132
7	80.85069668772786	5.985838895767534	8.14762354164997	5.015840874854635
8	80.50844546797232	5.149224803031765	8.073694006964423	6.268635722031492
9	74.72615813396621	7.3895186892882325	10.860117796572744	7.024205380172818
10	40.040285053966926	25.230089320617015	18.229354932097685	16.500270693318374
11	30.6338149111946	26.949605245725746	22.50614580147303	19.910434041606624
12	27.8235930080979	22.59062501533384	29.014725389397654	20.57105658717061
13	25.050335872343975	24.645522601665377	30.255416728683514	20.048724797307134
14	20.75964232498573	27.745410889558762	28.618826188719858	22.876120596735653
15	19.99425901179765	26.467836563388854	32.475837963470305	21.062066461343196
16	21.876599830223768	25.995145511970208	30.03289210049837	22.095362557307656
17	21.32621734301832	25.602926431934943	27.649237070100575	25.421619154946164
18	23.27161089511983	25.068818256015362	29.239294529050873	22.420276319813933
19	23.196209312177274	27.325795355851312	27.00750581050159	22.470489521469826
20	24.93756061976503	26.949278123890853	27.195600865564373	20.917560390779744
21	24.26368963988793	27.91117987939018	27.26854903474524	20.556581445976647
22	22.28681061117808	25.677101307996658	26.358496090076265	25.677591990748994
23	22.141813857862292	27.21490105382299	27.09533802317004	23.54794706514468
24	23.1902393386905	24.83173670617753	27.658723603312435	24.319300351819535
25	21.32564487980726	26.541766098074397	25.861598022875633	26.27099099924271
26	20.779187854620513	26.199842000153744	27.906273051866805	25.114697093358934
27	22.098306653821684	25.999888778576135	27.727173847263543	24.174630720338637
28	18.74146416462079	26.46072166347995	27.651772264320982	27.146041907578265
29	20.54979366790264	25.523599386973682	26.15216399271827	27.77444295240541
30	22.454951234312464	25.59761070211795	26.833068092045544	25.11436997152404
31	23.895186892882318	26.79062403396833	23.143869818594588	26.170319254554762
32	23.15221142538433	25.929802925450566	24.131859540426532	26.786126108738568
33	22.176570552819545	25.9466496999475	25.956054452700638	25.92072529453232
34	22.395660401738326	24.360599483474623	26.554932751928796	26.688807362858256
35	22.870068842790154	23.693516281671528	25.561954422114745	27.874460453423577
36	22.563392122579092	26.49719574807039	27.105724141427856	23.833687987922662
37	22.731450965254755	25.449424510911967	26.984525501600444	24.834599022232837
38	21.98921152188527	27.779349779928786	24.37523818558603	25.856200512599912
39	24.032659843995596	24.42994931247168	26.269355390068256	25.268035453464467
40	26.305993035576137	22.854694116550238	27.346567592366938	23.492745255506687
41	22.902045002150825	23.475162456881254	26.843617771220803	26.779174769747115
42	22.774794608377917	22.23160880154009	28.408486848884433	26.58510974119756
43	22.478749347800843	21.62128123809072	29.334159861169496	26.565809552938944
44	20.859659826003895	23.224341789977966	26.220368895293205	29.69562948872493
45	22.93737416031914	21.531568074871647	25.695338350291873	29.835719414517342
46	23.855114468108074	23.361978302008694	26.50946281687883	26.2734444130044
47	21.204282679062402	22.940072915456998	30.2371796863883	25.618464719092305
48	21.33562209577146	22.008675271061332	29.475721835218916	27.179980797948293
49	22.657112528275594	21.037859445561203	30.877275336812822	25.427752689350385
50	21.570086670930156	20.49483719964082	29.61074137257051	28.324334756858516
51	21.35802994146155	20.542433426617578	26.642601403679794	31.456935228241083
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	345.0
1	688.0
2	1031.0
3	1674.0
4	2317.0
5	1868.5
6	1420.0
7	1433.0
8	1446.0
9	1563.5
10	1681.0
11	1834.5
12	1988.0
13	2169.0
14	2350.0
15	2532.5
16	2715.0
17	2706.0
18	2697.0
19	2719.5
20	2742.0
21	3321.5
22	3901.0
23	4085.0
24	4269.0
25	5782.0
26	8436.5
27	9578.0
28	10995.5
29	12413.0
30	14147.0
31	15881.0
32	17186.5
33	18492.0
34	21506.0
35	24520.0
36	26072.5
37	27625.0
38	29826.5
39	32028.0
40	35586.0
41	39144.0
42	44146.0
43	49148.0
44	55473.5
45	61799.0
46	70080.5
47	78362.0
48	92013.5
49	105665.0
50	124690.5
51	143716.0
52	144095.5
53	144475.0
54	123831.0
55	103187.0
56	88341.0
57	73495.0
58	64922.5
59	56350.0
60	54387.0
61	52424.0
62	48604.0
63	44784.0
64	37526.5
65	30269.0
66	24241.5
67	18214.0
68	15881.5
69	13549.0
70	11705.0
71	9861.0
72	9230.0
73	8599.0
74	6931.5
75	4321.0
76	3378.0
77	2809.0
78	2240.0
79	1613.0
80	986.0
81	809.0
82	632.0
83	462.5
84	293.0
85	204.0
86	115.0
87	91.5
88	68.0
89	43.0
90	18.0
91	17.5
92	17.0
93	8.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1222786.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.494928805403084
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.49396599357466	18.282468609822292
2	13.156836325144067	7.234925561283545
3	5.797747550307199	4.782259683821897
4	3.033698447423097	3.336452913158397
5	1.8229736544212	2.506126542121819
6	1.2545687248341009	2.069656666247936
7	0.8540336682338475	1.643711643385481
8	0.6226000498191079	1.369467523521343
9	0.4943503577288373	1.2232915101611936
>10	5.660733474180687	37.275703162212274
>50	0.7116410029070583	12.39922850085467
>100	0.08820868437846123	4.520464768977433
>500	0.005066039303831849	0.8922778511342766
>1k	0.002980023119901088	1.430921982755648
>5k	5.960046239802176E-4	1.0330430805418698
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC	7285	0.595770641796684	No Hit
CCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC	5323	0.4353173817822579	No Hit
CTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCT	4409	0.36057004250948244	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC	2912	0.23814469580122768	No Hit
ACTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC	1901	0.15546465203232618	No Hit
GCCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG	1438	0.11760029964360078	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1340	0.10958581468875174	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1232	0.10075352514667325	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.40873873269729943	0.0
2	0.0	0.0	0.0	2.0133531133002833	0.0
3	0.0	0.0	0.0	2.719609154831671	0.0
4	0.0	0.0	0.0	3.508136337838346	0.0
5	0.0	0.0	0.0	5.5698217022438925	0.0
6	0.0	0.0	0.0	6.654966609038704	0.0
7	0.0	0.0	0.0	7.7832098175805084	0.0
8	0.0	0.0	0.0	9.66039846710708	0.0
9	0.0	0.0	0.0	10.31701377019364	0.0
10	0.0	0.0	0.0	11.961046331901084	0.0
11	0.0	0.0	0.0	14.386082274412693	0.0
12	0.0	0.0	0.0	16.18484346402396	0.0
13	0.0	0.0	0.0	16.918577739686256	0.0
14	0.0	0.0	0.0	17.183628206407334	0.0
15	0.0	0.0	0.0	17.72313389260263	0.0
16	0.0	0.0	0.0	18.903961936103293	0.0
17	0.0	0.0	0.0	20.291939881549183	0.0
18	0.0	0.0	0.0	21.929184665182625	0.0
19	0.0	0.0	0.0	22.80562584131647	0.0
20	0.0	0.0	0.0	23.667346534880185	0.0
21	3.2712183489179626E-4	0.0	0.0	24.746357907270774	0.0
22	4.906827523376944E-4	0.0	0.0	25.839599079479157	0.0
23	7.360241285065416E-4	0.0	0.0	26.899719165904745	0.0
24	0.001144926422121287	0.0	0.0	27.731998894328196	0.0
25	0.0019627310093507776	0.0	0.0	28.505396692471127	0.0
26	0.0019627310093507776	0.0	0.0	29.17068072418232	0.0
27	0.0019627310093507776	0.0	0.0	29.836128316810953	0.0
28	0.0019627310093507776	0.0	0.0	30.53338850788282	0.0
29	0.0019627310093507776	0.0	0.0	31.223288457669618	0.0
30	0.0019627310093507776	0.0	0.0	31.99627735351893	0.0
31	0.0019627310093507776	0.0	0.0	32.73033875101612	0.0
32	0.0019627310093507776	0.0	0.0	33.443382570621516	0.0
33	0.0020445114680737266	0.0	0.0	34.15519968334606	0.0
34	0.002535194220411421	0.0	0.0	34.82947956551678	0.0
35	0.002535194220411421	0.0	0.0	35.54661240805832	0.0
36	0.00261697467913437	0.0	0.0	36.2082980995857	0.0
37	0.00261697467913437	0.0	0.0	36.86123328202972	0.0
38	0.00261697467913437	0.0	0.0	37.54704420888038	0.0
39	0.00261697467913437	0.0	0.0	38.226394479491915	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCACG	20	7.033463E-4	45.000004	1
TACGATC	20	7.033463E-4	45.000004	11
CGTAAGG	160	0.0	45.000004	2
CGTATCG	25	3.890953E-5	45.0	2
GATCGAC	25	3.890953E-5	45.0	10
TTCGTCG	25	3.890953E-5	45.0	1
ATTAGTC	175	0.0	42.42857	31
CACAACG	655	0.0	41.221375	12
TTAGTCA	170	0.0	41.02941	32
GTATGCG	50	1.0822987E-9	40.5	1
TATTAGT	185	0.0	40.135136	30
CGTATTA	40	3.4584627E-7	39.375004	17
GTTAACG	40	3.4584627E-7	39.375004	1
CCGAACG	40	3.4584627E-7	39.375004	1
TCAACGG	80	0.0	39.375004	2
AACGAGC	710	0.0	38.66197	15
GCCGATA	70	0.0	38.57143	9
TGTTACG	70	0.0	38.57143	1
TCGATCA	125	0.0	37.800003	17
AACACGT	435	0.0	37.75862	41
>>END_MODULE