SRR3552440_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3552440_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	935229
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	5420	0.5795372042569252	RNA PCR Primer, Index 10 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	5308	0.5675615277113948	RNA PCR Primer, Index 35 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCT	3484	0.3725290811127542	TruSeq Adapter, Index 10 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	1762	0.18840305422522186	RNA PCR Primer, Index 35 (95% over 24bp)
ACTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	1276	0.1364371720722946	RNA PCR Primer, Index 35 (95% over 24bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1117	0.1194359884049789	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1097	0.11729747473613414	No Hit
CGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTG	1005	0.10746031185944833	TruSeq Adapter, Index 10 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGATCG	35	1.2116107E-7	45.000004	1
CCAACGG	35	1.2116107E-7	45.000004	2
ACTATCG	20	7.032612E-4	45.0	2
CCGTATC	20	7.032612E-4	45.0	5
TCTACGG	45	3.8380676E-10	45.0	2
CGCAGTA	20	7.032612E-4	45.0	37
TAATGCG	20	7.032612E-4	45.0	1
CCGTTAG	20	7.032612E-4	45.0	1
TACGTCG	20	7.032612E-4	45.0	32
CTTACGT	20	7.032612E-4	45.0	22
CGAATAT	215	0.0	43.953487	14
TCGAATG	125	0.0	41.399998	1
GCTACGA	235	0.0	41.17021	10
CACGGGC	230	0.0	41.08696	4
CTACGAA	230	0.0	41.08696	11
TACGAAT	230	0.0	41.08696	12
ACGTAGG	105	0.0	40.714287	2
GCGTAAG	105	0.0	40.714287	1
GACCGTC	50	1.0804797E-9	40.5	17
GCGATTA	50	1.0804797E-9	40.5	9