##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552440_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	935229
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.58637082468572	31.0	31.0	34.0	30.0	34.0
2	31.746324162317464	31.0	31.0	34.0	30.0	34.0
3	31.80061140105792	31.0	31.0	34.0	30.0	34.0
4	35.42403625208372	37.0	35.0	37.0	33.0	37.0
5	35.42354439393988	37.0	35.0	37.0	33.0	37.0
6	35.432608484125275	37.0	35.0	37.0	33.0	37.0
7	35.86659096328279	37.0	35.0	37.0	35.0	37.0
8	35.86971426249614	37.0	35.0	37.0	35.0	37.0
9	37.58753417612157	39.0	37.0	39.0	35.0	39.0
10	36.9964340284572	39.0	37.0	39.0	33.0	39.0
11	36.79375104920827	39.0	37.0	39.0	32.0	39.0
12	36.56971714948959	39.0	35.0	39.0	32.0	39.0
13	36.48819593917639	39.0	35.0	39.0	32.0	39.0
14	37.53100791356983	39.0	36.0	41.0	32.0	41.0
15	37.539041240166846	39.0	36.0	41.0	32.0	41.0
16	37.58189812334733	39.0	36.0	41.0	33.0	41.0
17	37.569430588657966	39.0	36.0	41.0	32.0	41.0
18	37.55052933559588	39.0	36.0	41.0	32.0	41.0
19	37.52916879181463	39.0	36.0	41.0	32.0	41.0
20	37.41713312996068	39.0	36.0	41.0	32.0	41.0
21	37.34853281923465	39.0	35.0	41.0	32.0	41.0
22	37.351121490030785	39.0	35.0	41.0	32.0	41.0
23	37.31133872024927	39.0	35.0	41.0	32.0	41.0
24	37.30717503413602	39.0	35.0	41.0	32.0	41.0
25	37.2494191262247	39.0	35.0	41.0	32.0	41.0
26	37.19933620535719	39.0	35.0	41.0	32.0	41.0
27	37.10504272215682	39.0	35.0	41.0	32.0	41.0
28	36.93969498379541	39.0	35.0	40.0	31.0	41.0
29	36.92167693687856	39.0	35.0	40.0	31.0	41.0
30	36.856620143301804	39.0	35.0	40.0	31.0	41.0
31	36.65522134151101	39.0	35.0	40.0	30.0	41.0
32	36.55314580706971	39.0	35.0	40.0	30.0	41.0
33	36.48083838289873	39.0	35.0	40.0	30.0	41.0
34	36.31649360744802	38.0	35.0	40.0	30.0	41.0
35	36.311551502359315	38.0	35.0	40.0	30.0	41.0
36	36.147297613739525	38.0	35.0	40.0	30.0	41.0
37	35.905148364732064	38.0	35.0	40.0	29.0	41.0
38	35.86765273531937	38.0	35.0	40.0	29.0	41.0
39	35.90003945557719	38.0	35.0	40.0	29.0	41.0
40	35.7822896852001	38.0	35.0	40.0	29.0	41.0
41	35.67788851714393	38.0	34.0	40.0	28.0	41.0
42	35.58199221794876	38.0	34.0	40.0	28.0	41.0
43	35.43028605828091	38.0	34.0	40.0	27.0	41.0
44	35.22975763155334	38.0	34.0	40.0	27.0	41.0
45	35.17096668302629	38.0	34.0	40.0	26.0	41.0
46	34.98025938032289	37.0	34.0	40.0	26.0	41.0
47	34.80831218877943	37.0	33.0	40.0	26.0	41.0
48	34.744284020277384	37.0	33.0	40.0	26.0	41.0
49	34.672166923822935	37.0	33.0	40.0	26.0	41.0
50	34.520174203323464	37.0	33.0	40.0	26.0	41.0
51	33.025985079590136	35.0	31.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	13.0
9	33.0
10	53.0
11	41.0
12	25.0
13	23.0
14	15.0
15	38.0
16	88.0
17	152.0
18	297.0
19	602.0
20	970.0
21	1588.0
22	2336.0
23	3588.0
24	5125.0
25	7163.0
26	8873.0
27	11152.0
28	13917.0
29	17450.0
30	21836.0
31	27574.0
32	34677.0
33	46475.0
34	71843.0
35	83251.0
36	85719.0
37	119204.0
38	178423.0
39	192652.0
40	33.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.5169664328202	19.936828306222328	25.33368832660236	12.212516934355115
2	29.782866014633846	25.710922137786575	25.586353716576365	18.91985813100321
3	32.136407232880934	23.907299709482917	26.75740380163575	17.198889256000403
4	29.10998268873185	26.98365854779952	24.830602986006635	19.075755777461993
5	25.080809085261468	29.475561600420857	24.57547830531346	20.86815100900421
6	25.121226993602637	35.16903346666966	23.87340426783173	15.836335271895974
7	80.19137558822491	6.050069020528662	8.398905508704285	5.359649882542137
8	79.38804292852339	5.751960215091705	8.268776951955083	6.591219904429824
9	73.34610025993634	7.9984688242131075	11.39977481451067	7.255656101339886
10	40.21132792075524	24.19311206132402	18.652971625131386	16.94258839278936
11	30.365397138027156	25.882217082661036	23.442066060825745	20.31031971848606
12	26.86368793097733	22.681075971767342	29.595746068609934	20.859490028645393
13	24.075814586587885	25.018792188864975	30.765940748201775	20.139452476345365
14	19.977673917297263	28.006509635607962	28.70997370697444	23.305842740120333
15	19.046778917249142	26.30339734974001	33.19465072190875	21.455173011102094
16	20.690012820389445	25.5094741501814	30.406456600468978	23.39405642896018
17	20.7050893417548	25.465848471336965	27.974752707625615	25.854309479282616
18	21.57685443885936	24.88673896981381	30.30231098479624	23.234095606530595
19	23.054139681297308	26.496505134036692	27.64071687255207	22.80863831211393
20	24.524795531361836	26.77654349897191	27.896483107345905	20.802177862320352
21	22.765761113053596	28.422985172615476	28.43816861966427	20.373085094666653
22	21.251586509828073	26.038007803436376	25.957171986754048	26.753233699981504
23	20.741764851175486	27.04289537642652	27.220712787990962	24.994626984407027
24	22.52699606192708	24.570025095457904	28.486178251529836	24.416800591085178
25	20.52107024055071	26.88111681737842	26.95692712693896	25.64088581513191
26	21.793272022146446	26.964732701830247	27.44194202703295	23.800053248990356
27	23.757176049930017	26.40551137742735	27.319405193808148	22.51790737883449
28	19.47619246195317	26.17850815147948	29.920800146274335	24.424499240293017
29	22.219370870663763	25.114597601229217	28.62977944439276	24.036252083714256
30	24.736294533210582	24.825791330251732	27.82270438577076	22.615209750766923
31	24.515920699636133	26.748208192859718	26.386264754407744	22.34960635309641
32	24.455400762807823	24.74185466874958	28.328249017085653	22.47449555135694
33	23.714726553603448	24.38247744670022	27.65023325837843	24.252562741317902
34	22.68909539802551	22.858786457648343	30.834373185604807	23.61774495872134
35	24.13526526658177	21.916343483788463	29.60547630580318	24.342914943826592
36	25.40661164270997	23.97476981573497	28.20293211609135	22.41568642546371
37	23.154649823733013	24.778102475436498	30.876394979197606	21.190852721632883
38	20.3752236083355	25.944020127690653	30.995296339185373	22.68545992478847
39	23.347436830979365	21.47655814779054	30.78721895920678	24.388786062023314
40	26.180325888097993	21.20165221566055	30.61528246023166	22.00273943600979
41	22.34682628532691	22.57297410580724	28.2491240113384	26.831075597527448
42	23.558721981461225	21.88854280609348	29.080578125785234	25.472157086660058
43	24.272450918438157	20.793623807644973	30.202335470777747	24.731589803139123
44	21.851867296672793	21.99889011140587	30.476065220389874	25.673177371531462
45	23.743489562449412	20.638902343704054	29.900805043470633	25.716803050375898
46	24.14638553765976	22.054170689745504	30.489965559237366	23.30947821335737
47	21.282060329609113	22.218622390879666	32.69744629390235	23.801870985608872
48	20.976145949280873	21.067246631573656	32.35988191127521	25.596725507870268
49	22.53533626523557	19.716240621280992	32.983472497110334	24.764950616373103
50	21.364286180176194	19.875132186876154	32.37036062825255	26.390221004695107
51	20.770848637071776	19.415351748074535	29.319663953962078	30.494135660891608
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	335.0
1	598.0
2	861.0
3	1402.0
4	1943.0
5	1471.5
6	1000.0
7	1036.0
8	1072.0
9	1177.5
10	1283.0
11	1340.5
12	1398.0
13	1591.0
14	1784.0
15	1801.5
16	1819.0
17	1824.0
18	1829.0
19	1994.0
20	2159.0
21	2389.5
22	2620.0
23	3130.0
24	3640.0
25	4473.0
26	6502.5
27	7699.0
28	8447.0
29	9195.0
30	10924.5
31	12654.0
32	14826.0
33	16998.0
34	18225.0
35	19452.0
36	21620.0
37	23788.0
38	24652.5
39	25517.0
40	30062.5
41	34608.0
42	40909.5
43	47211.0
44	54777.0
45	62343.0
46	73693.5
47	85044.0
48	97918.5
49	110793.0
50	110741.0
51	110689.0
52	94710.0
53	78731.0
54	66992.0
55	55253.0
56	49863.0
57	44473.0
58	41371.5
59	38270.0
60	36103.0
61	33936.0
62	30875.0
63	27814.0
64	24220.0
65	20626.0
66	17496.0
67	14366.0
68	12008.0
69	9650.0
70	8101.5
71	6553.0
72	5662.0
73	4771.0
74	4295.0
75	2903.0
76	1987.0
77	1479.5
78	972.0
79	755.5
80	539.0
81	403.0
82	267.0
83	168.0
84	69.0
85	57.0
86	45.0
87	34.5
88	24.0
89	16.5
90	9.0
91	8.0
92	7.0
93	4.5
94	2.0
95	4.0
96	6.0
97	3.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	935229.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.262659432031363
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.97754199187203	18.929634589108588
2	12.921268976896874	7.303788490474174
3	5.798631488117554	4.916542407615589
4	3.019374039841482	3.4134216074382597
5	1.857627803286554	2.625075097788022
6	1.2258018113397964	2.078665147503829
7	0.8466302035707238	1.6749614775873525
8	0.6572871906921347	1.4861347215654768
9	0.4866054072778446	1.2377486613310773
>10	5.4570579256588	37.45457320976565
>50	0.6680112977282154	11.805939565918463
>100	0.07623630704504288	3.914086312329684
>500	0.00490629698804718	0.976094940567608
>1k	0.002264444763714083	1.0390275178744477
>5k	7.548149212380277E-4	1.1443062531318218
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	5420	0.5795372042569252	RNA PCR Primer, Index 10 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	5308	0.5675615277113948	RNA PCR Primer, Index 35 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCT	3484	0.3725290811127542	TruSeq Adapter, Index 10 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	1762	0.18840305422522186	RNA PCR Primer, Index 35 (95% over 24bp)
ACTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	1276	0.1364371720722946	RNA PCR Primer, Index 35 (95% over 24bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1117	0.1194359884049789	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1097	0.11729747473613414	No Hit
CGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTG	1005	0.10746031185944833	TruSeq Adapter, Index 10 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4393576332641524	0.0
2	0.0	0.0	0.0	2.1801077597037732	0.0
3	0.0	0.0	0.0	2.89020122344367	0.0
4	0.0	0.0	0.0	3.6627392863138333	0.0
5	0.0	0.0	0.0	5.652733180857309	0.0
6	0.0	0.0	0.0	6.7481868077230285	0.0
7	0.0	0.0	0.0	7.9289671299756534	0.0
8	0.0	0.0	0.0	9.848390073447252	0.0
9	0.0	0.0	0.0	10.525336575320056	0.0
10	0.0	0.0	0.0	12.227379604353587	0.0
11	0.0	0.0	0.0	14.743234010065985	0.0
12	0.0	0.0	0.0	16.626943775267875	0.0
13	0.0	0.0	0.0	17.40151342612344	0.0
14	0.0	0.0	0.0	17.71298794199068	0.0
15	0.0	0.0	0.0	18.292097443513835	0.0
16	0.0	0.0	0.0	19.59819466676076	0.0
17	0.0	0.0	0.0	21.10317366121025	0.0
18	0.0	0.0	0.0	22.91085926548471	0.0
19	0.0	0.0	0.0	23.848918286323457	0.0
20	0.0	0.0	0.0	24.73084132335503	0.0
21	6.415541006534229E-4	0.0	0.0	25.792185657202676	0.0
22	6.415541006534229E-4	0.0	0.0	26.859090126589315	0.0
23	7.4847978409566E-4	0.0	0.0	27.935938684536087	0.0
24	0.0010692568344223715	0.0	0.0	28.86811679278551	0.0
25	0.0018177366185180314	0.0	0.0	29.667386276516233	0.0
26	0.0020315879854025057	0.0	0.0	30.358126191553083	0.0
27	0.0020315879854025057	0.0	0.0	31.04993536342436	0.0
28	0.0020315879854025057	0.0	0.0	31.741423758245308	0.0
29	0.002138513668844743	0.0	0.0	32.45493884385535	0.0
30	0.002138513668844743	0.0	0.0	33.25014515161527	0.0
31	0.002138513668844743	0.0	0.0	34.001511929163875	0.0
32	0.00224543935228698	0.0	0.0	34.73641215146237	0.0
33	0.00224543935228698	0.0	0.0	35.437737709160004	0.0
34	0.00224543935228698	0.0	0.0	36.13884941549075	0.0
35	0.00224543935228698	0.0	0.0	36.87203882685417	0.0
36	0.00224543935228698	0.0	0.0	37.56887350584723	0.0
37	0.00224543935228698	0.0	0.0	38.258009535632446	0.0
38	0.00224543935228698	0.0	0.0	38.89175806139459	0.0
39	0.00224543935228698	0.0	0.0	39.56389290751249	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGATCG	35	1.2116107E-7	45.000004	1
CCAACGG	35	1.2116107E-7	45.000004	2
ACTATCG	20	7.032612E-4	45.0	2
CCGTATC	20	7.032612E-4	45.0	5
TCTACGG	45	3.8380676E-10	45.0	2
CGCAGTA	20	7.032612E-4	45.0	37
TAATGCG	20	7.032612E-4	45.0	1
CCGTTAG	20	7.032612E-4	45.0	1
TACGTCG	20	7.032612E-4	45.0	32
CTTACGT	20	7.032612E-4	45.0	22
CGAATAT	215	0.0	43.953487	14
TCGAATG	125	0.0	41.399998	1
GCTACGA	235	0.0	41.17021	10
CACGGGC	230	0.0	41.08696	4
CTACGAA	230	0.0	41.08696	11
TACGAAT	230	0.0	41.08696	12
ACGTAGG	105	0.0	40.714287	2
GCGTAAG	105	0.0	40.714287	1
GACCGTC	50	1.0804797E-9	40.5	17
GCGATTA	50	1.0804797E-9	40.5	9
>>END_MODULE