FastQCFastQC Report
Sat 18 Jun 2016
SRR3552439_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552439_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1418925
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC60270.4247581796078017Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCT39950.2815511743044911Illumina Single End Adapter 1 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC34310.24180277322621Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC26180.18450587592719842Illumina Single End Adapter 1 (95% over 21bp)
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC20690.14581461317546734No Hit
ACTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC17700.12474232253290345Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCCA207.033846E-445.00000420
TTAACGG850.042.352942
TATTGCG603.6379788E-1241.2499961
TAAACGG800.039.3750042
TATCGCG403.4589902E-739.3750041
TGCGTAG800.039.3750041
TTTAGCG1200.039.3749961
TAATACG356.2493255E-638.5714261
CTATACG356.2493255E-638.5714261
CCCTCGT3250.038.0769214
CGTAAGG2900.038.0172422
TATACGG950.037.8947372
CACAACG3950.037.59493612
TCGTTAG601.5643309E-1037.4999961
CGAACTA301.14005714E-437.49999630
CCGTAAG301.14005714E-437.4999961
TCGTAAG601.5643309E-1037.4999961
GTATACG301.14005714E-437.4999961
TATAGCG301.14005714E-437.4999961
TCGACGC301.14005714E-437.49999617