##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552439_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1418925
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.715011716616452	31.0	31.0	34.0	30.0	34.0
2	31.877404373028877	33.0	31.0	34.0	30.0	34.0
3	31.945217682400408	33.0	31.0	34.0	30.0	34.0
4	35.552743802526564	37.0	35.0	37.0	33.0	37.0
5	35.524334267138855	37.0	35.0	37.0	33.0	37.0
6	35.527358387511676	37.0	35.0	37.0	33.0	37.0
7	35.88323413852036	37.0	35.0	37.0	35.0	37.0
8	35.88559014747079	37.0	35.0	37.0	35.0	37.0
9	37.612448860933455	39.0	37.0	39.0	35.0	39.0
10	37.01412195852494	39.0	37.0	39.0	33.0	39.0
11	36.84410592526032	39.0	37.0	39.0	33.0	39.0
12	36.66605141216061	39.0	35.0	39.0	32.0	39.0
13	36.63335553323819	39.0	35.0	39.0	32.0	39.0
14	37.73030639392498	40.0	37.0	41.0	33.0	41.0
15	37.72937611219761	40.0	36.0	41.0	33.0	41.0
16	37.74825096463872	40.0	36.0	41.0	33.0	41.0
17	37.72074563489966	39.0	36.0	41.0	33.0	41.0
18	37.6989925471748	39.0	36.0	41.0	33.0	41.0
19	37.68156104092887	39.0	36.0	41.0	33.0	41.0
20	37.563183395880685	39.0	36.0	41.0	32.0	41.0
21	37.51176277815952	39.0	36.0	41.0	32.0	41.0
22	37.48238067551139	39.0	36.0	41.0	32.0	41.0
23	37.4374438395264	39.0	36.0	41.0	32.0	41.0
24	37.41769297179203	39.0	36.0	41.0	32.0	41.0
25	37.33326497172155	39.0	35.0	41.0	32.0	41.0
26	37.26938492168367	39.0	35.0	41.0	32.0	41.0
27	37.1827658262417	39.0	35.0	41.0	32.0	41.0
28	37.08141515583981	39.0	35.0	41.0	31.0	41.0
29	37.031880472893214	39.0	35.0	41.0	31.0	41.0
30	36.93548919076061	39.0	35.0	41.0	31.0	41.0
31	36.82964638722977	39.0	35.0	40.0	31.0	41.0
32	36.74777736666843	39.0	35.0	40.0	31.0	41.0
33	36.66143453670913	39.0	35.0	40.0	30.0	41.0
34	36.56780943319767	39.0	35.0	40.0	30.0	41.0
35	36.52384939302641	39.0	35.0	40.0	30.0	41.0
36	36.40969325369558	38.0	35.0	40.0	30.0	41.0
37	36.272261747449654	38.0	35.0	40.0	30.0	41.0
38	36.21292316366263	38.0	35.0	40.0	30.0	41.0
39	36.18918195112497	38.0	35.0	40.0	30.0	41.0
40	36.103450147118416	38.0	35.0	40.0	30.0	41.0
41	36.01735398276865	38.0	35.0	40.0	30.0	41.0
42	35.92796307063446	38.0	35.0	40.0	29.0	41.0
43	35.849773596208394	38.0	35.0	40.0	29.0	41.0
44	35.77729689729901	38.0	34.0	40.0	29.0	41.0
45	35.71226668076184	38.0	34.0	40.0	29.0	41.0
46	35.60665503814508	38.0	34.0	40.0	29.0	41.0
47	35.47413992987649	38.0	34.0	40.0	28.0	41.0
48	35.38865126768504	37.0	34.0	40.0	28.0	41.0
49	35.31482213647656	37.0	34.0	40.0	28.0	41.0
50	35.211103476223194	37.0	34.0	40.0	27.0	41.0
51	34.0095297496344	36.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	20.0
9	48.0
10	78.0
11	63.0
12	39.0
13	34.0
14	30.0
15	52.0
16	109.0
17	229.0
18	469.0
19	782.0
20	1353.0
21	2130.0
22	3218.0
23	4611.0
24	6759.0
25	9002.0
26	11791.0
27	14779.0
28	18606.0
29	23572.0
30	30048.0
31	37829.0
32	48911.0
33	64888.0
34	101052.0
35	118837.0
36	132323.0
37	183969.0
38	278291.0
39	324956.0
40	46.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.718519301583946	19.92170128794686	25.26053174057826	11.099247669890937
2	29.969801081804885	24.79750515354934	26.3321176242578	18.90057614038797
3	32.665292386842154	24.70144651761016	25.794245643709147	16.83901545183854
4	29.73934492661698	25.827439787162813	25.027186073964447	19.406029212255756
5	25.82116743309195	29.541378155998377	23.964127772785734	20.673326638123932
6	25.796853251581304	35.588068432087674	22.89317617210212	15.721902144228906
7	82.11674330919534	6.097151012209948	7.093891502369752	4.692214176224959
8	82.58886128583258	4.940077875856722	7.138220836196417	5.332840002114277
9	76.94458833271666	6.92002748559649	9.633349190408232	6.502034991278609
10	41.14227319978153	24.88574096587205	17.231848053984532	16.740137780361895
11	30.556724280705467	26.759906267068377	21.962683017072784	20.720686435153375
12	26.0736120654721	24.070334936659794	28.756840565921383	21.09921243194672
13	24.13940130732773	25.025212749088215	29.68514896840918	21.15023697517487
14	21.117959018270874	27.319696248920838	28.213612417851543	23.348732314956745
15	20.82921930334584	26.15226315696742	31.37565410433955	21.64286343534718
16	22.881688602286943	25.276318339588066	28.939866448191413	22.902126609933575
17	22.625156368377468	25.03909649911024	27.59384745493948	24.74189967757281
18	23.948552601441232	23.91345560899977	28.959881600507426	23.17811018905157
19	24.26322744331096	26.309353912292753	26.607889775710486	22.8195288686858
20	26.03696460348503	26.173194495833112	26.356079426326268	21.433761474355588
21	25.66534524375848	26.452631393484506	26.76857480134609	21.113448561410927
22	23.254083196786297	25.010624240181826	25.296333491904083	26.438959071127787
23	22.354669908557533	26.81438412882993	26.292228271402646	24.538717691209893
24	23.493630741582535	24.89715805979879	27.25746603943126	24.351745159187413
25	22.540796729918778	26.38011170428317	25.81059604982645	25.2684955159716
26	22.139929876491003	26.33655760522931	26.585901298518245	24.93761121976144
27	23.50272213119087	27.143788431382916	25.511919234631854	23.84157020279437
28	20.35519847772081	28.120936624557324	26.845111616188312	24.678753281533556
29	22.424370562221398	27.088112479517946	25.034938421692477	25.452578536568176
30	23.136317987208628	27.117219021442292	25.259263174586394	24.48719981676269
31	22.931374103634795	30.47342178057332	22.06663495251687	24.528569163275016
32	22.82460313265324	29.09611149285551	23.307856299663477	24.771429074827775
33	21.897633772045737	28.260549359550364	23.895343305671545	25.946473562732354
34	20.813996511443523	28.16667547615272	25.726165935479322	25.293162076924432
35	22.310692954173053	26.868086755818666	24.368870800077524	26.45234948993076
36	21.91736702080801	29.974452490441706	24.226650457212326	23.88153003153796
37	21.910671811406523	30.75673485208873	25.072008739010165	22.26058459749458
38	22.057825466462287	29.68120231865673	24.833236428986734	23.427735785894253
39	22.78844900188523	25.828637877266242	26.046901703754603	25.33601141709393
40	23.561569498035485	25.40550064309248	27.017636591081278	24.015293267790756
41	21.449618549253838	26.684497066441143	25.88558239512307	25.980301989181953
42	22.573356590376516	26.087213911940378	26.883732403051606	24.4556970946315
43	22.76265482671741	25.175960674454252	27.814014130415632	24.247370368412707
44	21.907641348203747	25.865990098137676	25.45116901879944	26.775199534859134
45	22.80797082298219	24.40107828109308	25.368923656993854	27.42202723893088
46	23.048857409658723	25.46054231196152	25.692267033141285	25.798333245238474
47	20.489807424634847	26.525785365681763	28.59404126363268	24.390365946050707
48	21.14727698786053	25.795514209701004	27.50518878728615	25.552020015152316
49	21.990380041228395	24.770442412389663	28.823722184047785	24.415455362334164
50	21.175678770900504	24.652959106365735	27.906548971932978	26.26481315080078
51	21.05460119456631	24.041580774177636	25.664147153655055	29.239670877601
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	268.0
1	598.0
2	928.0
3	1587.5
4	2247.0
5	1782.0
6	1317.0
7	1381.5
8	1446.0
9	1483.5
10	1521.0
11	1643.0
12	1765.0
13	1884.0
14	2003.0
15	2135.0
16	2267.0
17	2228.0
18	2189.0
19	2383.0
20	2577.0
21	2999.0
22	3421.0
23	3944.5
24	4468.0
25	5680.0
26	8702.5
27	10513.0
28	12641.0
29	14769.0
30	18130.5
31	21492.0
32	22861.5
33	24231.0
34	27189.5
35	30148.0
36	32643.0
37	35138.0
38	39553.0
39	43968.0
40	49921.0
41	55874.0
42	64350.0
43	72826.0
44	90405.5
45	107985.0
46	128940.5
47	149896.0
48	151492.0
49	153088.0
50	134310.0
51	115532.0
52	105841.5
53	96151.0
54	86093.5
55	76036.0
56	72058.0
57	68080.0
58	66236.5
59	64393.0
60	62259.0
61	60125.0
62	55627.0
63	51129.0
64	45179.0
65	39229.0
66	33940.5
67	28652.0
68	24705.0
69	20758.0
70	18060.5
71	15363.0
72	12982.5
73	10602.0
74	8995.5
75	6447.0
76	5505.0
77	4359.5
78	3214.0
79	2533.0
80	1852.0
81	1383.0
82	914.0
83	634.0
84	354.0
85	287.5
86	221.0
87	161.5
88	102.0
89	69.5
90	37.0
91	34.0
92	31.0
93	19.5
94	8.0
95	8.5
96	9.0
97	5.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1418925.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.99485227863207
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.47778385051075	17.800698755621237
2	12.267792937231384	6.377989303763513
3	4.994486210078166	3.8949279371594057
4	2.584180004364363	2.687015098993856
5	1.4777453347574223	1.9206885841228445
6	0.9796801288251222	1.5279984137472173
7	0.7177390837069412	1.3060265018893873
8	0.582222734467359	1.2107835180592175
9	0.4487197556741313	1.0497963386927627
>10	6.558784641160645	42.5939451500753
>50	0.826410605535261	13.287718573950938
>100	0.07739419103056096	3.831551741589717
>500	0.00488805414773634	0.9435932053351803
>1k	0.0019009099463419099	1.1418134283157433
>5k	2.7155856376312996E-4	0.42545344868370855
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC	6027	0.4247581796078017	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCT	3995	0.2815511743044911	Illumina Single End Adapter 1 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC	3431	0.24180277322621	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC	2618	0.18450587592719842	Illumina Single End Adapter 1 (95% over 21bp)
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	2069	0.14581461317546734	No Hit
ACTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC	1770	0.12474232253290345	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.409517768733372E-4	0.0	0.0	0.32270909315150553	0.0
2	1.409517768733372E-4	0.0	0.0	1.432070053033106	0.0
3	1.409517768733372E-4	0.0	0.0	1.94182215409553	0.0
4	1.409517768733372E-4	0.0	0.0	2.5505928784114733	0.0
5	1.409517768733372E-4	0.0	0.0	4.063851154923622	0.0
6	1.409517768733372E-4	0.0	0.0	4.943531194390119	0.0
7	1.409517768733372E-4	0.0	0.0	5.831456912803707	0.0
8	1.409517768733372E-4	0.0	0.0	7.2666279049280265	0.0
9	1.409517768733372E-4	0.0	0.0	7.770812410803954	0.0
10	1.409517768733372E-4	0.0	0.0	9.122398999242384	0.0
11	1.409517768733372E-4	0.0	0.0	11.048716457881847	0.0
12	1.409517768733372E-4	0.0	0.0	12.503268319326251	0.0
13	1.409517768733372E-4	0.0	0.0	13.067004950931162	0.0
14	1.409517768733372E-4	0.0	0.0	13.296122064238773	0.0
15	1.409517768733372E-4	0.0	0.0	13.741388727381645	0.0
16	1.409517768733372E-4	0.0	0.0	14.693729407826348	0.0
17	1.409517768733372E-4	0.0	0.0	15.812322709093152	0.0
18	1.409517768733372E-4	0.0	0.0	17.04374790774706	0.0
19	1.409517768733372E-4	0.0	0.0	17.755131525626794	0.0
20	1.409517768733372E-4	0.0	0.0	18.43557622848283	0.0
21	3.5237944218334305E-4	0.0	0.0	19.29326779075709	0.0
22	4.2285533062001165E-4	0.0	0.0	20.161883115739027	0.0
23	6.342829959300174E-4	0.0	0.0	21.01055376429339	0.0
24	9.161865496766919E-4	0.0	0.0	21.716863118205683	0.0
25	0.001057138326550029	0.0	0.0	22.360589883186215	0.0
26	0.001057138326550029	0.0	0.0	22.922212238138027	0.0
27	0.0011276142149866976	0.0	0.0	23.461070881124794	0.0
28	0.0011276142149866976	0.0	0.0	24.023961802068467	0.0
29	0.0011276142149866976	0.0	0.0	24.596437443839527	0.0
30	0.0011276142149866976	0.0	0.0	25.239811829377874	0.0
31	0.0011276142149866976	0.0	0.0	25.846750180594466	0.0
32	0.0011276142149866976	0.0	0.0	26.435505752594395	0.0
33	0.0011276142149866976	0.0	0.0	27.028489877900522	0.0
34	0.001620945434043378	0.0	0.0	27.578906566590906	0.0
35	0.001620945434043378	0.0	0.0	28.162376446958085	0.0
36	0.0017618972109167152	0.0	0.0	28.716739785400918	0.0
37	0.0017618972109167152	0.0	0.0	29.24305372024596	0.0
38	0.0017618972109167152	0.0	0.0	29.78846662085734	0.0
39	0.0017618972109167152	0.0	0.0	30.32894620927815	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTCCA	20	7.033846E-4	45.000004	20
TTAACGG	85	0.0	42.35294	2
TATTGCG	60	3.6379788E-12	41.249996	1
TAAACGG	80	0.0	39.375004	2
TATCGCG	40	3.4589902E-7	39.375004	1
TGCGTAG	80	0.0	39.375004	1
TTTAGCG	120	0.0	39.374996	1
TAATACG	35	6.2493255E-6	38.571426	1
CTATACG	35	6.2493255E-6	38.571426	1
CCCTCGT	325	0.0	38.07692	14
CGTAAGG	290	0.0	38.017242	2
TATACGG	95	0.0	37.894737	2
CACAACG	395	0.0	37.594936	12
TCGTTAG	60	1.5643309E-10	37.499996	1
CGAACTA	30	1.14005714E-4	37.499996	30
CCGTAAG	30	1.14005714E-4	37.499996	1
TCGTAAG	60	1.5643309E-10	37.499996	1
GTATACG	30	1.14005714E-4	37.499996	1
TATAGCG	30	1.14005714E-4	37.499996	1
TCGACGC	30	1.14005714E-4	37.499996	17
>>END_MODULE