FastQCFastQC Report
Sat 18 Jun 2016
SRR3552437_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552437_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences775391
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC70070.9036731145963779No Hit
CCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC49590.6395483053066131No Hit
CTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGCT46330.5975050007028712Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC29790.3841932650752975No Hit
ACTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC19300.24890668062951463No Hit
GCCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG14180.18287547830707346No Hit
ACCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG10450.13477071567763876No Hit
AGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG9800.1263878481952976No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG9680.12484024189086539No Hit
CGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG9210.11877878386517254No Hit
GGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG9090.11723117756074032No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTCA207.031864E-445.00000416
CGAACCG207.031864E-445.00000426
CAACGTG207.031864E-445.0000043
TTTACGC207.031864E-445.00000436
ACCGAGT207.031864E-445.00000419
CACGCAA207.031864E-445.00000440
TATGCGC207.031864E-445.00000438
ATCGTCA302.1645847E-645.00000437
TAACCGA207.031864E-445.00000417
ACGGCAC207.031864E-445.00000428
TCGCTAG207.031864E-445.0000042
TCGTCAA302.1645847E-645.00000438
CGAATAT600.045.00000414
GTATACG302.1645847E-645.0000041
CGATCCA207.031864E-445.00000410
ATGCACG207.031864E-445.0000041
GGTTACG551.8189894E-1245.0000041
TCGCGAT207.031864E-445.00000423
AGTCGTT207.031864E-445.00000423
TACGTAG207.031864E-445.0000041