##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552437_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	775391
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.590754857871705	31.0	31.0	34.0	30.0	34.0
2	31.737658806976093	31.0	31.0	34.0	30.0	34.0
3	31.800579320626625	31.0	31.0	34.0	30.0	34.0
4	35.434980545299084	37.0	35.0	37.0	33.0	37.0
5	35.42591028268319	37.0	35.0	37.0	33.0	37.0
6	35.45676955239357	37.0	35.0	37.0	33.0	37.0
7	35.85208365843813	37.0	35.0	37.0	35.0	37.0
8	35.86426203038209	37.0	35.0	37.0	35.0	37.0
9	37.59449103742499	39.0	37.0	39.0	35.0	39.0
10	37.016213755382765	39.0	37.0	39.0	33.0	39.0
11	36.842260227420745	39.0	37.0	39.0	33.0	39.0
12	36.63360807644143	39.0	35.0	39.0	32.0	39.0
13	36.51753502426518	39.0	35.0	39.0	32.0	39.0
14	37.54163512344095	39.0	36.0	41.0	33.0	41.0
15	37.57153745658642	39.0	36.0	41.0	33.0	41.0
16	37.606279928449	39.0	36.0	41.0	33.0	41.0
17	37.59796541357844	39.0	36.0	41.0	33.0	41.0
18	37.57509566141469	39.0	36.0	41.0	33.0	41.0
19	37.57680189736533	39.0	36.0	41.0	32.0	41.0
20	37.47148083998911	39.0	36.0	41.0	32.0	41.0
21	37.42247330701543	39.0	35.0	41.0	32.0	41.0
22	37.4062363375381	39.0	35.0	41.0	32.0	41.0
23	37.381806082350714	39.0	35.0	41.0	32.0	41.0
24	37.354974458047614	39.0	35.0	41.0	32.0	41.0
25	37.30825480306065	39.0	35.0	41.0	32.0	41.0
26	37.248482378567715	39.0	35.0	41.0	32.0	41.0
27	37.1802484166053	39.0	35.0	41.0	32.0	41.0
28	37.13842822524378	39.0	35.0	41.0	32.0	41.0
29	37.069739009093475	39.0	35.0	41.0	31.0	41.0
30	36.95119494551781	39.0	35.0	40.0	31.0	41.0
31	36.90389880718244	39.0	35.0	40.0	31.0	41.0
32	36.75748751275163	39.0	35.0	40.0	31.0	41.0
33	36.601576494955445	39.0	35.0	40.0	30.0	41.0
34	36.54396685027296	39.0	35.0	40.0	30.0	41.0
35	36.48132103674146	39.0	35.0	40.0	30.0	41.0
36	36.36721344457184	38.0	35.0	40.0	30.0	41.0
37	36.28131613598816	38.0	35.0	40.0	30.0	41.0
38	36.20754432279972	38.0	35.0	40.0	30.0	41.0
39	36.174856298306274	38.0	35.0	40.0	30.0	41.0
40	36.128314618044314	38.0	35.0	40.0	30.0	41.0
41	36.08461795403867	38.0	35.0	40.0	30.0	41.0
42	36.02271886054906	38.0	35.0	40.0	30.0	41.0
43	35.9281884881305	38.0	35.0	40.0	30.0	41.0
44	35.838999936806076	38.0	35.0	40.0	29.0	41.0
45	35.78395802891703	38.0	35.0	40.0	29.0	41.0
46	35.68284388134502	38.0	34.0	40.0	29.0	41.0
47	35.58086952260214	38.0	34.0	40.0	29.0	41.0
48	35.51127882577951	38.0	34.0	40.0	28.0	41.0
49	35.46200562039023	38.0	34.0	40.0	28.0	41.0
50	35.35100226853291	38.0	34.0	40.0	28.0	41.0
51	34.17132130757257	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	5.0
9	24.0
10	44.0
11	46.0
12	31.0
13	19.0
14	15.0
15	22.0
16	69.0
17	116.0
18	261.0
19	465.0
20	783.0
21	1304.0
22	1934.0
23	2607.0
24	3828.0
25	5093.0
26	6275.0
27	7796.0
28	9616.0
29	12117.0
30	15829.0
31	20625.0
32	26589.0
33	36283.0
34	58305.0
35	68126.0
36	71250.0
37	98617.0
38	152625.0
39	174646.0
40	24.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.18582495798894	20.619532597102623	24.569152853205672	11.625489591702767
2	30.137956205320926	25.313035616869424	25.285178703389644	19.263829474420003
3	31.707873833975377	24.427933777926235	27.037326974390986	16.826865413707406
4	29.675221920295698	26.22496263175611	25.160467428690815	18.93934801925738
5	24.32669453217796	29.92967419018276	25.568003755524632	20.17562752211465
6	25.593023390779617	34.30398340966042	24.184443719362232	15.918549480197733
7	77.68764404023261	6.583775153438717	9.764493010623028	5.964087795705651
8	76.28822103944978	5.73220478442489	9.982834466740007	7.99673970938533
9	70.6033472145021	8.361200994079116	13.089396188503608	7.946055602915174
10	38.575892678661475	25.075607016331112	20.292213863715208	16.0562864412922
11	29.532455238711826	25.616366452538138	24.306446683028305	20.54473162572173
12	26.137135973979582	22.733046940188885	31.35566443252501	19.774152653306526
13	23.500659667187264	25.972702804133657	31.313750095113303	19.212887433565776
14	18.988484519423103	29.007429799933192	28.7915387204649	23.212546960178802
15	17.22730854497924	26.63353069612621	35.24234869891448	20.89681205998006
16	19.008087532612578	26.484315654940538	31.080706379104218	23.426890433342663
17	18.669419686325995	25.811235879704565	28.619109584712742	26.900234849256698
18	20.082900111040754	25.357787232505924	31.885719591793045	22.67359306466028
19	21.726586973539803	27.62038764958582	27.554743348839487	23.098282028034888
20	23.508397698709423	26.706784061202672	29.02574314120231	20.759075098885592
21	22.07905430937424	27.746904464973156	29.22009669960059	20.95394452605202
22	19.048325296527814	27.40733384834232	26.0211944683392	27.523146386790664
23	19.791305289847315	27.221105223042308	27.9157225193483	25.07186696776207
24	22.215243664164273	24.550323643168415	28.818492863600433	24.415939829066886
25	18.911362138585567	27.351748988574798	27.509991733202995	26.226897139636645
26	18.49724848495791	27.674682837432986	30.456763104033964	23.37130557357514
27	20.247462248078712	28.968868609514427	27.682033967379038	23.101635175027823
28	16.768701210099163	27.65417705389926	28.960743676416158	26.616378059585422
29	18.81824782593556	27.60774886476629	28.17584934568495	25.398153963613197
30	19.878745046047737	30.510542423112984	25.702000668050058	23.908711862789225
31	20.009904680348367	29.304570210384178	23.0764865725808	27.609038536686654
32	21.930871005724853	30.569996298641588	23.869892737986383	23.629239957647176
33	20.227988202081274	30.21752896280715	24.702246995386844	24.85223583972473
34	18.22370907064952	25.847088759090575	28.361691069408852	27.567511100851057
35	20.522936170267645	26.840007170575873	26.758628872401154	25.878427786755324
36	19.89770322327703	28.841706958166913	28.01709073228861	23.243499086267445
37	19.4879744541786	28.7242178462221	27.685129179987904	24.102678519611395
38	19.87822917727959	30.14272799142626	25.293174669295876	24.685868161998272
39	20.984767684948626	26.418671354194206	28.476600837512944	24.11996012334422
40	22.95370980576251	23.852095265485413	28.693136752941417	24.501058175810655
41	19.811424171804934	24.927423712681733	27.201502209852833	28.0596499056605
42	21.059310721945444	23.634656579712686	29.952243448789062	25.353789249552804
43	22.169976179759633	24.181993342713547	29.081843869737977	24.566186607788847
44	19.991849273463323	24.944447382030486	27.15378434879951	27.909918995706683
45	20.73521616835893	24.546067725831225	25.977990459007135	28.740725646802712
46	21.916039778640712	25.28479180181354	27.71118055277918	25.087987866766575
47	18.390205715568015	23.81559755013922	32.02049030747068	25.77370642682208
48	19.840957658781182	22.921210073369437	30.385444246838045	26.852388021011336
49	20.630494808425684	21.73664641451861	32.58923562434952	25.043623152706186
50	19.678587964007836	21.749156232146106	30.6427337949499	27.929522008896157
51	19.76809119528083	20.84341964247715	26.741218301476287	32.64727086076573
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	363.0
1	483.5
2	604.0
3	1115.5
4	1627.0
5	1262.5
6	898.0
7	976.0
8	1054.0
9	1140.5
10	1227.0
11	1333.0
12	1439.0
13	1528.5
14	1618.0
15	1691.5
16	1765.0
17	1811.0
18	1857.0
19	1905.5
20	1954.0
21	2241.0
22	2528.0
23	2373.0
24	2218.0
25	3584.5
26	5472.5
27	5994.0
28	7188.0
29	8382.0
30	9402.5
31	10423.0
32	12543.5
33	14664.0
34	16225.5
35	17787.0
36	17544.0
37	17301.0
38	19811.0
39	22321.0
40	27793.5
41	33266.0
42	41252.5
43	49239.0
44	61832.5
45	74426.0
46	90300.5
47	106175.0
48	103583.5
49	100992.0
50	83420.5
51	65849.0
52	56554.5
53	47260.0
54	41333.5
55	35407.0
56	32112.5
57	28818.0
58	27121.5
59	25425.0
60	24775.0
61	24125.0
62	21528.5
63	18932.0
64	16728.0
65	14524.0
66	12559.5
67	10595.0
68	9130.5
69	7666.0
70	6329.0
71	4992.0
72	3922.5
73	2853.0
74	2429.5
75	1379.0
76	752.0
77	634.5
78	517.0
79	396.5
80	276.0
81	228.0
82	180.0
83	110.0
84	40.0
85	46.5
86	53.0
87	40.5
88	28.0
89	18.0
90	8.0
91	5.5
92	3.0
93	2.5
94	2.0
95	4.5
96	7.0
97	3.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	775391.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.246948438256275
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.41304189705114	17.8230577975845
2	13.355535919849332	7.277951971468287
3	6.012481609631328	4.914653292107666
4	3.3424561832318034	3.642869251265913
5	2.1102400816759515	2.874880134888318
6	1.3882027812723734	2.2694573761903385
7	0.9427103432188567	1.7980186079726548
8	0.6934358453293168	1.511520857834124
9	0.5326691394782127	1.3062247720211881
>10	5.39808325335875	34.815524014205835
>50	0.6997352356428662	12.10216668976325
>100	0.10097805227828088	5.186476789108712
>500	0.007111130442132457	1.3808387346974529
>1k	0.0028444521768529827	2.191258025763105
>5k	4.740753628088304E-4	0.9051016851286299
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC	7007	0.9036731145963779	No Hit
CCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC	4959	0.6395483053066131	No Hit
CTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGCT	4633	0.5975050007028712	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC	2979	0.3841932650752975	No Hit
ACTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC	1930	0.24890668062951463	No Hit
GCCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG	1418	0.18287547830707346	No Hit
ACCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG	1045	0.13477071567763876	No Hit
AGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG	980	0.1263878481952976	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	968	0.12484024189086539	No Hit
CGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG	921	0.11877878386517254	No Hit
GGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG	909	0.11723117756074032	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.645867697716378	0.0
2	0.0	0.0	0.0	2.9801738735683028	0.0
3	0.0	0.0	0.0	4.05356781288408	0.0
4	0.0	0.0	0.0	5.185770791768282	0.0
5	0.0	0.0	0.0	7.904528167079577	0.0
6	0.0	0.0	0.0	9.307949150815524	0.0
7	0.0	0.0	0.0	10.792232563958054	0.0
8	0.0	0.0	0.0	13.098165957562056	0.0
9	0.0	0.0	0.0	13.933228525995272	0.0
10	0.0	0.0	0.0	16.050611884842613	0.0
11	0.0	0.0	0.0	18.914070449618322	0.0
12	0.0	0.0	0.0	21.073497113069408	0.0
13	0.0	0.0	0.0	21.950474018914328	0.0
14	0.0	0.0	0.0	22.295461257610675	0.0
15	0.0	0.0	0.0	22.962995443589104	0.0
16	0.0	0.0	0.0	24.36976957431799	0.0
17	0.0	0.0	0.0	26.102572766513926	0.0
18	0.0	0.0	0.0	28.140770269451153	0.0
19	0.0	0.0	0.0	29.165285642985282	0.0
20	0.0	0.0	0.0	30.14762874472363	0.0
21	2.579343840720359E-4	0.0	0.0	31.329484092541698	0.0
22	0.0020634750725762874	0.0	0.0	32.479097642350766	0.0
23	0.002579343840720359	0.0	0.0	33.67075449676357	0.0
24	0.004126950145152575	0.0	0.0	34.57042962840683	0.0
25	0.00567455644958479	0.0	0.0	35.380988430353206	0.0
26	0.00567455644958479	0.0	0.0	36.08901831463094	0.0
27	0.00567455644958479	0.0	0.0	36.76583813843596	0.0
28	0.00567455644958479	0.0	0.0	37.465098253655256	0.0
29	0.00567455644958479	0.0	0.0	38.206659607862356	0.0
30	0.00567455644958479	0.0	0.0	39.0479126015133	0.0
31	0.00567455644958479	0.0	0.0	39.75104173249367	0.0
32	0.00567455644958479	0.0	0.0	40.49363482423706	0.0
33	0.00567455644958479	0.0	0.0	41.15459168342166	0.0
34	0.00567455644958479	0.0	0.0	41.80755257670001	0.0
35	0.006061458025692844	0.0	0.0	42.46760666554035	0.0
36	0.006706293985872934	0.0	0.0	43.181053171883605	0.0
37	0.00696422836994497	0.0	0.0	43.81079997059548	0.0
38	0.00696422836994497	0.0	0.0	44.449187571173766	0.0
39	0.00696422836994497	0.0	0.0	45.07558122289271	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTCA	20	7.031864E-4	45.000004	16
CGAACCG	20	7.031864E-4	45.000004	26
CAACGTG	20	7.031864E-4	45.000004	3
TTTACGC	20	7.031864E-4	45.000004	36
ACCGAGT	20	7.031864E-4	45.000004	19
CACGCAA	20	7.031864E-4	45.000004	40
TATGCGC	20	7.031864E-4	45.000004	38
ATCGTCA	30	2.1645847E-6	45.000004	37
TAACCGA	20	7.031864E-4	45.000004	17
ACGGCAC	20	7.031864E-4	45.000004	28
TCGCTAG	20	7.031864E-4	45.000004	2
TCGTCAA	30	2.1645847E-6	45.000004	38
CGAATAT	60	0.0	45.000004	14
GTATACG	30	2.1645847E-6	45.000004	1
CGATCCA	20	7.031864E-4	45.000004	10
ATGCACG	20	7.031864E-4	45.000004	1
GGTTACG	55	1.8189894E-12	45.000004	1
TCGCGAT	20	7.031864E-4	45.000004	23
AGTCGTT	20	7.031864E-4	45.000004	23
TACGTAG	20	7.031864E-4	45.000004	1
>>END_MODULE