##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552429_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1180212
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.551497527562844	31.0	31.0	34.0	30.0	34.0
2	31.715207945691112	31.0	31.0	34.0	30.0	34.0
3	31.766981694814152	31.0	31.0	34.0	30.0	34.0
4	35.39503241790458	37.0	35.0	37.0	33.0	37.0
5	35.38845817531087	37.0	35.0	37.0	33.0	37.0
6	35.39583651072858	37.0	35.0	37.0	33.0	37.0
7	35.823905366154555	37.0	35.0	37.0	35.0	37.0
8	35.857340884519054	37.0	35.0	37.0	35.0	37.0
9	37.55841577614869	39.0	37.0	39.0	35.0	39.0
10	36.93639363097478	39.0	37.0	39.0	33.0	39.0
11	36.741127017857806	39.0	37.0	39.0	32.0	39.0
12	36.53357362914459	39.0	35.0	39.0	32.0	39.0
13	36.464328442686565	39.0	35.0	39.0	32.0	39.0
14	37.506508152772554	39.0	36.0	41.0	32.0	41.0
15	37.50596164078996	39.0	36.0	41.0	32.0	41.0
16	37.52881770393794	39.0	36.0	41.0	32.0	41.0
17	37.496975967029655	39.0	36.0	41.0	32.0	41.0
18	37.47264982901377	39.0	36.0	41.0	32.0	41.0
19	37.45830749051865	39.0	36.0	41.0	32.0	41.0
20	37.370617312821764	39.0	35.0	41.0	32.0	41.0
21	37.30721175517619	39.0	35.0	41.0	32.0	41.0
22	37.29451912029364	39.0	35.0	41.0	32.0	41.0
23	37.24726405086544	39.0	35.0	41.0	32.0	41.0
24	37.21149505343108	39.0	35.0	41.0	32.0	41.0
25	37.15301827129363	39.0	35.0	41.0	32.0	41.0
26	37.101158944325256	39.0	35.0	41.0	32.0	41.0
27	37.05251937787448	39.0	35.0	40.0	31.0	41.0
28	36.990209386110294	39.0	35.0	40.0	31.0	41.0
29	36.93136402612412	39.0	35.0	40.0	31.0	41.0
30	36.83730634835097	39.0	35.0	40.0	31.0	41.0
31	36.7120042839761	39.0	35.0	40.0	31.0	41.0
32	36.60478964796155	39.0	35.0	40.0	30.0	41.0
33	36.52043954814898	39.0	35.0	40.0	30.0	41.0
34	36.403057247342005	39.0	35.0	40.0	30.0	41.0
35	36.34027615377576	38.0	35.0	40.0	30.0	41.0
36	36.26655295828207	38.0	35.0	40.0	30.0	41.0
37	36.18274513392509	38.0	35.0	40.0	30.0	41.0
38	36.130453681203036	38.0	35.0	40.0	30.0	41.0
39	36.087627477097335	38.0	35.0	40.0	30.0	41.0
40	35.93727398128472	38.0	35.0	40.0	29.0	41.0
41	35.86275347141022	38.0	35.0	40.0	29.0	41.0
42	35.862372183980504	38.0	35.0	40.0	29.0	41.0
43	35.82511786018105	38.0	35.0	40.0	29.0	41.0
44	35.74536693407625	38.0	34.0	40.0	29.0	41.0
45	35.632859181231844	38.0	34.0	40.0	28.0	41.0
46	35.46085364324376	38.0	34.0	40.0	28.0	41.0
47	35.34943383053214	38.0	34.0	40.0	28.0	41.0
48	35.30083917126753	37.0	34.0	40.0	28.0	41.0
49	35.28227979379976	37.0	34.0	40.0	28.0	41.0
50	35.16836127746541	37.0	34.0	40.0	27.0	41.0
51	33.98332502974042	36.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	12.0
9	39.0
10	89.0
11	48.0
12	44.0
13	29.0
14	21.0
15	37.0
16	97.0
17	215.0
18	356.0
19	695.0
20	1233.0
21	1929.0
22	2909.0
23	4337.0
24	6335.0
25	8493.0
26	10473.0
27	13110.0
28	16315.0
29	20297.0
30	26100.0
31	33386.0
32	42911.0
33	56868.0
34	89365.0
35	101438.0
36	108149.0
37	149918.0
38	226783.0
39	258140.0
40	40.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.29248643464056	20.750424500005085	25.81824282417057	11.138846241183789
2	30.63034437880652	25.33138114169319	24.825116165570254	19.21315831393004
3	32.5167851199615	24.153965558730125	26.47270151464313	16.856547806665244
4	30.47249138290409	25.993719772379876	24.66251825943136	18.871270585284677
5	25.553375156327846	29.59849586345504	24.05923681508068	20.78889216513643
6	25.90424432220652	34.88635939983664	23.732261661464214	15.47713461649263
7	80.19957431376736	6.409695885146058	8.391289022650168	4.9994407784364165
8	80.20940305640003	5.217282996614168	8.343162075966012	6.230151871019783
9	73.73302423632364	7.397484519730353	11.222390553561564	7.647100690384439
10	40.09805018081497	24.709967361796018	18.475833155399197	16.716149301989812
11	30.36098599234714	26.044303904722206	23.084750875266476	20.509959227664183
12	26.688764391482206	23.606691001277735	30.12348628890403	19.581058318336027
13	23.703707469505478	25.103710180882754	31.255825224620658	19.936757124991104
14	19.951076586240436	28.59121920468526	29.139679989696766	22.318024219377534
15	18.94235950829173	25.568118270276862	34.34501598017983	21.14450624125157
16	21.23152450576676	25.56388174328002	30.89919438202628	22.30539936892694
17	21.074942467963385	25.500926952106912	27.84736979457928	25.576760785350427
18	22.458761646212714	25.363748207949083	29.966226406781153	22.21126373905705
19	23.402744591649636	27.1122476300868	27.215957810969556	22.26904996729401
20	24.819269758314608	27.461337454626793	27.00370780842764	20.715684978630957
21	22.841828417267408	28.006663209660637	28.440398843597592	20.711109529474367
22	20.6048574323935	26.03227216805116	27.094454216700047	26.26841618285528
23	21.040372407669132	27.228497930880213	27.652150630564677	24.07897903088598
24	22.393519130461307	24.903068262312196	28.591473396305073	24.111939210921427
25	21.131034085401605	26.73833175734529	26.619539540353763	25.51109461689934
26	20.315163716349264	27.13393864831064	27.374403920651545	25.176493714688547
27	22.52519038952324	26.381277262051224	26.773156009259353	24.320376339166184
28	18.481171179415224	26.322897920034706	28.501235371272283	26.69469552927779
29	21.346758040080935	24.91908233436027	27.317719189433763	26.416440436125033
30	24.127529630269816	25.85933713603997	25.543376952615294	24.469756281074925
31	22.182201163858696	29.52842370692723	22.629154762025806	25.66022036718827
32	21.52198079667043	28.821008428994112	24.770380236771018	24.88663053756444
33	22.335817632764282	27.99225901787137	24.761229338457834	24.910694010906514
34	18.973116694288823	27.634441947717868	27.353644938367005	26.038796419626305
35	20.320078087665607	26.794423374783516	26.382548220150277	26.502950317400604
36	21.874036190108217	28.942935675963298	25.383320962674503	23.799707171253978
37	21.384378399812913	28.451244352709516	27.167746133745464	22.996631113732107
38	19.21968256550518	29.760839577974124	26.981254215344364	24.03822364117633
39	21.38014187281607	27.024466790712175	28.628754833877302	22.966636502594447
40	21.218984385856103	27.63817009147509	28.15434854077064	22.98849698189817
41	19.008788251602255	28.55122638983505	26.338488339383094	26.101497019179604
42	20.95182899343508	24.94958532873755	28.592066510084628	25.50651916774275
43	22.600261647907324	22.617546678054453	29.88420724412224	24.897984429915983
44	21.14162540289372	23.52018112000217	27.294248829871243	28.04394464723287
45	21.772952655963504	23.803689506631013	27.021501221814386	27.401856615591097
46	22.227786194344745	26.247318278410997	26.967273676254777	24.557621850989484
47	18.937021484275707	25.882892226142424	30.40563898689388	24.774447302687992
48	18.749173877235613	24.699460774843843	30.386744076487954	26.16462127143259
49	20.892263423859443	22.589077216635655	31.89664229816338	24.622017061341523
50	19.78144604528678	22.5949236238913	30.476643179360995	27.146987151460923
51	18.808654716271313	22.707191589307683	27.35050990838934	31.133643786031662
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	327.0
1	675.5
2	1024.0
3	1738.5
4	2453.0
5	1877.0
6	1301.0
7	1375.5
8	1450.0
9	1551.0
10	1652.0
11	1815.0
12	1978.0
13	2008.0
14	2038.0
15	2192.0
16	2346.0
17	2330.5
18	2315.0
19	2528.0
20	2741.0
21	2886.5
22	3032.0
23	3469.5
24	3907.0
25	6150.0
26	8840.5
27	9288.0
28	11288.0
29	13288.0
30	15470.0
31	17652.0
32	19620.5
33	21589.0
34	22361.0
35	23133.0
36	25717.0
37	28301.0
38	31607.0
39	34913.0
40	41522.5
41	48132.0
42	57456.5
43	66781.0
44	83126.5
45	99472.0
46	120541.0
47	141610.0
48	142407.5
49	143205.0
50	122037.5
51	100870.0
52	89032.0
53	77194.0
54	69861.0
55	62528.0
56	58906.5
57	55285.0
58	51939.0
59	48593.0
60	46287.0
61	43981.0
62	38763.5
63	33546.0
64	28843.0
65	24140.0
66	20666.5
67	17193.0
68	14049.5
69	10906.0
70	9755.0
71	8604.0
72	7093.5
73	5583.0
74	4792.0
75	3225.0
76	2449.0
77	1992.5
78	1536.0
79	1171.5
80	807.0
81	599.0
82	391.0
83	284.5
84	178.0
85	117.0
86	56.0
87	48.0
88	40.0
89	24.0
90	8.0
91	5.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1180212.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.72698187162522
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.58170567050811	17.528010585659317
2	13.186165509877066	7.048528130774681
3	5.8722451113770076	4.708421659125393
4	3.202487198701138	3.423712692151886
5	1.9355643214301184	2.5865896265113677
6	1.3488163179250112	2.162987356640044
7	0.9559896961062998	1.7885503494105508
8	0.7302640978136867	1.5614204243012124
9	0.5711062124760011	1.3737550848856765
>10	5.77125128073454	36.67987045871677
>50	0.7410411509560341	12.71768645829234
>100	0.09286806822136778	4.686547048314614
>500	0.006042785260575549	1.0922016001298451
>1k	0.0041345372835516914	2.1758179125631294
>5k	3.180413295039762E-4	0.4659006125232844
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	5481	0.4644080893941089	No Hit
CCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	4808	0.4073844360165801	No Hit
CTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT	4034	0.34180299810542514	No Hit
TCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	3145	0.2664775481015275	No Hit
ACTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	2024	0.1714946128322708	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1998	0.1692916187939116	No Hit
GCCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG	1529	0.1295529955635089	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	1392	0.11794491159215464	No Hit
ACCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG	1213	0.10277814494345085	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3733227589619492	0.0
2	0.0	0.0	0.0	1.7990835544800425	0.0
3	0.0	0.0	0.0	2.570978773305135	0.0
4	0.0	0.0	0.0	3.469376688256008	0.0
5	0.0	0.0	0.0	5.662457253442602	0.0
6	0.0	0.0	0.0	6.8486848125590996	0.0
7	0.0	0.0	0.0	8.041775545410486	0.0
8	0.0	0.0	0.0	9.991679460978197	0.0
9	0.0	0.0	0.0	10.65613635516331	0.0
10	0.0	0.0	0.0	12.359474399514664	0.0
11	0.0	0.0	0.0	15.038908263939021	0.0
12	0.0	0.0	0.0	17.09633523468665	0.0
13	0.0	0.0	0.0	17.851284345524363	0.0
14	0.0	0.0	0.0	18.14453674424595	0.0
15	0.0	0.0	0.0	18.7075711821266	0.0
16	0.0	0.0	0.0	19.95404215513823	0.0
17	0.0	0.0	0.0	21.420558340365968	0.0
18	0.0	0.0	0.0	23.148298780219147	0.0
19	0.0	0.0	0.0	24.087791007039414	0.0
20	0.0	0.0	0.0	24.986782035769846	0.0
21	6.778443194951415E-4	0.0	0.0	26.156910792298333	0.0
22	0.0010167664792427122	0.0	0.0	27.289334458554904	0.0
23	0.0010167664792427122	0.0	0.0	28.397440459849587	0.0
24	0.0012709580990533905	0.0	0.0	29.27643508115491	0.0
25	0.00220299403835921	0.0	0.0	30.068157246325235	0.0
26	0.002287724578296103	0.0	0.0	30.728377613513505	0.0
27	0.0023724551182329953	0.0	0.0	31.375718938631366	0.0
28	0.0023724551182329953	0.0	0.0	32.09262403703741	0.0
29	0.0023724551182329953	0.0	0.0	32.786567159120565	0.0
30	0.0023724551182329953	0.0	0.0	33.60557255815057	0.0
31	0.002457185658169888	0.0	0.0	34.3623857408669	0.0
32	0.002457185658169888	0.0	0.0	35.10555730665338	0.0
33	0.002457185658169888	0.0	0.0	35.851016597018166	0.0
34	0.002711377277980566	0.0	0.0	36.54267199452302	0.0
35	0.002965568897791244	0.0	0.0	37.249324697596705	0.0
36	0.002965568897791244	0.0	0.0	37.94479296939872	0.0
37	0.002965568897791244	0.0	0.0	38.603996570107746	0.0
38	0.003050299437728137	0.0	0.0	39.28548430282017	0.0
39	0.003050299437728137	0.0	0.0	39.98891724537626	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGGT	35	1.2119563E-7	45.000004	4
TCGATTG	25	3.890869E-5	45.000004	1
TACGCAA	25	3.890869E-5	45.000004	28
TGCGATA	25	3.890869E-5	45.000004	39
GCGATAT	35	1.2119563E-7	45.000004	9
TTAACGG	25	3.890869E-5	45.000004	2
CAATACG	25	3.890869E-5	45.000004	1
ACCTTAC	25	3.890869E-5	45.000004	24
AGTCGAC	25	3.890869E-5	45.000004	18
CGTGCAT	20	7.0333644E-4	45.0	18
ATTACGC	20	7.0333644E-4	45.0	26
TAGCGAT	20	7.0333644E-4	45.0	11
ATATGCG	40	6.8157533E-9	45.0	1
CTGCGTA	20	7.0333644E-4	45.0	18
ACGATAG	40	6.8157533E-9	45.0	1
AACGCAT	20	7.0333644E-4	45.0	15
ATAACGG	45	3.8562575E-10	45.0	2
ATGCACG	40	6.8157533E-9	45.0	1
TATCGAG	20	7.0333644E-4	45.0	1
TAATGCG	20	7.0333644E-4	45.0	1
>>END_MODULE