FastQCFastQC Report
Sat 18 Jun 2016
SRR3552427_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552427_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences895618
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC68480.7646116982910125No Hit
CCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC56780.6339756458668763No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT42900.4789988588884993No Hit
TCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC28140.3141964542918968No Hit
ACTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC18670.2084594101503096No Hit
GCCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG14930.1667005352728507No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA13750.15352527528477544No Hit
ACCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG11200.12505331514105344No Hit
CGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG9870.11020323396805334No Hit
GGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG9600.10718855583518867No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGAT207.0324505E-445.00000427
ACGCATA406.8121153E-945.00000431
ATTCGGA207.0324505E-445.00000422
CGCGACA207.0324505E-445.00000435
CCCGTTG207.0324505E-445.00000418
TGCGCTA207.0324505E-445.0000042
TATTACG207.0324505E-445.0000041
ACGCTAT207.0324505E-445.00000443
TCCCGTT207.0324505E-445.00000417
TACTCGC207.0324505E-445.00000445
ATGCGTC207.0324505E-445.00000412
TATAGCG207.0324505E-445.0000041
TATACGG406.8121153E-945.0000042
TCGAACG207.0324505E-445.0000041
TAGTACG207.0324505E-445.00000436
CGCATCC351.2115197E-745.011
ATTCGTG253.8901144E-545.01
GATATAC253.8901144E-545.09
TTGCGAC253.8901144E-545.02
CGACGGA302.164963E-644.99999617