##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552427_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	895618
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.538495206661768	31.0	31.0	34.0	30.0	34.0
2	31.696327005486715	31.0	31.0	34.0	30.0	34.0
3	31.755041770040354	31.0	31.0	34.0	30.0	34.0
4	35.40281347628118	37.0	35.0	37.0	33.0	37.0
5	35.38317228997184	37.0	35.0	37.0	33.0	37.0
6	35.40192470450572	37.0	35.0	37.0	33.0	37.0
7	35.81969991670556	37.0	35.0	37.0	35.0	37.0
8	35.817329486455165	37.0	35.0	37.0	35.0	37.0
9	37.53905348039007	39.0	37.0	39.0	35.0	39.0
10	36.949967508469015	39.0	37.0	39.0	33.0	39.0
11	36.765429011029255	39.0	37.0	39.0	32.0	39.0
12	36.545297213767476	39.0	35.0	39.0	32.0	39.0
13	36.44670718989569	39.0	35.0	39.0	32.0	39.0
14	37.45795305587873	39.0	36.0	41.0	32.0	41.0
15	37.47312693581415	39.0	36.0	41.0	32.0	41.0
16	37.51396689213482	39.0	36.0	41.0	32.0	41.0
17	37.494781257187775	39.0	36.0	41.0	32.0	41.0
18	37.49002923121241	39.0	36.0	41.0	32.0	41.0
19	37.47896982865463	39.0	36.0	41.0	32.0	41.0
20	37.36867057160531	39.0	36.0	41.0	32.0	41.0
21	37.295185000748084	39.0	35.0	41.0	32.0	41.0
22	37.27459809874299	39.0	35.0	41.0	32.0	41.0
23	37.22517412557586	39.0	35.0	40.0	32.0	41.0
24	37.21436259655344	39.0	35.0	41.0	32.0	41.0
25	37.15275039134988	39.0	35.0	41.0	32.0	41.0
26	37.09679126591917	39.0	35.0	41.0	31.0	41.0
27	37.030985308468566	39.0	35.0	41.0	31.0	41.0
28	36.98059887139383	39.0	35.0	40.0	31.0	41.0
29	36.95167359298272	39.0	35.0	40.0	31.0	41.0
30	36.82704568242264	39.0	35.0	40.0	31.0	41.0
31	36.730236551744156	39.0	35.0	40.0	31.0	41.0
32	36.65169078781356	39.0	35.0	40.0	30.0	41.0
33	36.55267312626589	39.0	35.0	40.0	30.0	41.0
34	36.47532765085114	39.0	35.0	40.0	30.0	41.0
35	36.44904412372239	39.0	35.0	40.0	30.0	41.0
36	36.339327704445424	38.0	35.0	40.0	30.0	41.0
37	36.238538082084105	38.0	35.0	40.0	30.0	41.0
38	36.1724462884846	38.0	35.0	40.0	30.0	41.0
39	36.13554886123325	38.0	35.0	40.0	30.0	41.0
40	35.99519437974672	38.0	35.0	40.0	29.0	41.0
41	35.96796513692222	38.0	35.0	40.0	29.0	41.0
42	35.94461254686708	38.0	35.0	40.0	29.0	41.0
43	35.85348440964786	38.0	35.0	40.0	29.0	41.0
44	35.72194841997369	38.0	34.0	40.0	29.0	41.0
45	35.65460162703295	38.0	34.0	40.0	29.0	41.0
46	35.496994254246786	38.0	34.0	40.0	28.0	41.0
47	35.39765837667398	38.0	34.0	40.0	28.0	41.0
48	35.354482603074075	38.0	34.0	40.0	28.0	41.0
49	35.32599277817105	38.0	34.0	40.0	28.0	41.0
50	35.17811946611167	37.0	34.0	40.0	27.0	41.0
51	33.98894171398967	36.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	8.0
9	43.0
10	50.0
11	52.0
12	21.0
13	16.0
14	13.0
15	27.0
16	69.0
17	170.0
18	301.0
19	582.0
20	958.0
21	1441.0
22	2230.0
23	3253.0
24	4822.0
25	6362.0
26	7992.0
27	10027.0
28	12319.0
29	15585.0
30	19938.0
31	25164.0
32	32131.0
33	42579.0
34	66839.0
35	78623.0
36	80961.0
37	111462.0
38	169333.0
39	202204.0
40	41.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.20848844038418	20.548492772588315	24.52161524221264	11.721403544814866
2	29.702283786167765	24.593744207910067	26.03085243932123	19.67311956660094
3	31.57819516802923	24.76468762351806	26.39774993356543	17.259367274887286
4	29.49103300737591	25.90959538553267	24.74548300726426	19.853888599827158
5	24.415989852816715	29.908845065641827	24.523959991871536	21.151205089669926
6	25.055771545457993	34.43979464459178	24.150586522378962	16.353847287571263
7	78.53571500349479	6.148491879350348	9.483284168026994	5.832508949127865
8	76.78932312660085	6.033599146064505	9.457156957542166	7.719920769792478
9	71.28563740344656	7.757883383317441	12.645346565165058	8.311132648070942
10	38.364458954598945	24.39466379639534	20.102097099433017	17.138780149572696
11	29.83537624299646	25.68137308540025	23.818301999289876	20.664948672313418
12	26.08243693181691	22.59735735547968	31.241667764604998	20.078537948098408
13	23.795859395411885	25.430931490881157	31.686500271321034	19.086708842385928
14	19.377122835851893	29.255106529792833	28.567201641771376	22.80056899258389
15	18.23869104908566	26.3285239912552	34.640103258308784	20.79268170135035
16	20.3059786650112	26.35811249885554	29.61229006116447	23.723618774968795
17	19.55621704789319	26.4525724136853	27.597591830445566	26.393618707975946
18	21.09727584751535	25.96475282988953	30.409951564171333	22.528019758423792
19	22.07369659832652	26.846490356379615	27.49576270240214	23.584050342891725
20	23.706200634645576	26.666949525355676	28.670258972017088	20.95659086798166
21	22.740498739417923	28.262384186115064	28.3618685644996	20.63524850996742
22	20.32116371042118	26.258628120471005	25.952470807866746	27.467737361241063
23	20.357004883778576	27.54020129117548	27.877956896801987	24.224836928243963
24	22.29510795897358	24.498391055115015	28.837852745255233	24.368648240656174
25	20.06793074726055	26.919289250551014	26.562887302399012	26.44989269978942
26	18.884948716975316	26.612127045235805	28.15888023688671	26.344044000902173
27	21.55215728134093	26.39641007661749	28.667914222358192	23.38351841968339
28	17.09255508486877	26.097287012989913	31.22078832716627	25.589369574975045
29	19.467004906109526	24.426262089417587	29.380494809170877	26.726238195302017
30	20.586678695604597	26.428678298113706	28.80804092816357	24.176602078118126
31	20.28163793045696	26.931124653591155	26.8223729313167	25.964864484635193
32	19.904914818594534	25.270595276111017	27.940706863863834	26.883783041430608
33	19.5031810437039	26.38870589916683	28.48033424964661	25.627778807482652
34	17.623250090998617	23.68565616144383	30.428151287714183	28.26294245984337
35	17.89725083685232	22.609304413265477	29.08996916095925	30.40347558892296
36	19.331009425893626	26.065800374713326	30.406043648073172	24.19714655131987
37	18.405279929612846	24.56035943895723	32.9622673952511	24.07209323617882
38	18.026993651311162	27.402307680283332	29.65818016163141	24.91251850677409
39	20.353655241408724	24.253755507370332	30.387397305547676	25.005191945673268
40	21.985935968236458	22.60818786580886	29.05155992845164	26.354316237503046
41	17.750536501052906	23.168136415302058	27.556726193533404	31.524600890111632
42	19.634263715110684	21.58118751521296	31.27907210440165	27.505476665274703
43	21.376077747432497	20.848397419435518	32.55003807426827	25.225486758863713
44	19.707732537756055	22.677413808119088	30.122887213075217	27.49196644104964
45	20.643734270637704	21.62484452076667	28.849911457786693	28.881509750808938
46	21.495325015799146	23.452632707247957	29.0703179257228	25.981724351230103
47	17.146930946005998	22.196963437536983	33.53807091862826	27.11803469782876
48	17.64211974301544	21.295351366319125	32.85563711314422	28.20689177752122
49	20.19867845443035	20.060784843538205	34.21458702259222	25.525949679439226
50	18.55791196693233	20.73707763801085	32.62350689691364	28.081503498143178
51	18.650808715322828	19.690761016415482	28.614878218168904	33.043552050092785
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	255.0
1	474.0
2	693.0
3	1262.5
4	1832.0
5	1396.5
6	961.0
7	1021.0
8	1081.0
9	1179.5
10	1278.0
11	1321.0
12	1364.0
13	1496.0
14	1628.0
15	1683.5
16	1739.0
17	1816.0
18	1893.0
19	2191.5
20	2490.0
21	2224.0
22	1958.0
23	2820.5
24	3683.0
25	4403.0
26	5310.5
27	5498.0
28	7038.5
29	8579.0
30	10136.5
31	11694.0
32	14311.0
33	16928.0
34	17569.0
35	18210.0
36	20913.0
37	23616.0
38	25433.5
39	27251.0
40	32457.5
41	37664.0
42	46512.5
43	55361.0
44	69420.0
45	83479.0
46	100255.5
47	117032.0
48	115429.0
49	113826.0
50	95223.5
51	76621.0
52	65239.0
53	53857.0
54	47592.0
55	41327.0
56	38806.0
57	36285.0
58	34479.5
59	32674.0
60	30318.0
61	27962.0
62	26014.0
63	24066.0
64	21295.5
65	18525.0
66	15800.5
67	13076.0
68	11040.0
69	9004.0
70	7459.5
71	5915.0
72	5066.5
73	4218.0
74	3490.0
75	2392.5
76	2023.0
77	1621.0
78	1219.0
79	892.0
80	565.0
81	385.5
82	206.0
83	162.5
84	119.0
85	82.5
86	46.0
87	30.5
88	15.0
89	10.5
90	6.0
91	5.5
92	5.0
93	5.0
94	5.0
95	2.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	895618.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.785590729068627
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.31682844383924	17.76335425146535
2	13.062336298252319	6.997647881008878
3	5.804442122786504	4.664262333345768
4	3.268183894072422	3.501609448558306
5	1.945450947706168	2.6055026434368056
6	1.271344279438498	2.0432224526869356
7	0.8755422223243426	1.6416340943239336
8	0.6620196886085621	1.4186070746923518
9	0.5044103002448999	1.215983507569786
>10	5.3352952281707395	34.23103647464849
>50	0.8387963983472876	14.315714879790068
>100	0.10406591984069356	5.322718875344319
>500	0.007940772999892279	1.4257502596669662
>1k	0.0025076125262817723	1.4507143227877053
>5k	8.358708420939241E-4	1.4022415006743418
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	6848	0.7646116982910125	No Hit
CCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	5678	0.6339756458668763	No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT	4290	0.4789988588884993	No Hit
TCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	2814	0.3141964542918968	No Hit
ACTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	1867	0.2084594101503096	No Hit
GCCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG	1493	0.1667005352728507	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1375	0.15352527528477544	No Hit
ACCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG	1120	0.12505331514105344	No Hit
CGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG	987	0.11020323396805334	No Hit
GGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG	960	0.10718855583518867	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1165474566165486E-4	0.0	0.0	0.5332630652800636	0.0
2	1.1165474566165486E-4	0.0	0.0	2.652805102175258	0.0
3	1.1165474566165486E-4	0.0	0.0	3.6249829726512868	0.0
4	1.1165474566165486E-4	0.0	0.0	4.651201740027556	0.0
5	1.1165474566165486E-4	0.0	0.0	7.36441205960577	0.0
6	1.1165474566165486E-4	0.0	0.0	8.722915350071125	0.0
7	1.1165474566165486E-4	0.0	0.0	10.12809032422305	0.0
8	1.1165474566165486E-4	0.0	0.0	12.401269291148681	0.0
9	1.1165474566165486E-4	0.0	0.0	13.178051356716814	0.0
10	1.1165474566165486E-4	0.0	0.0	15.192972896927039	0.0
11	1.1165474566165486E-4	0.0	0.0	17.840083607073552	0.0
12	1.1165474566165486E-4	0.0	0.0	19.87286990658964	0.0
13	1.1165474566165486E-4	0.0	0.0	20.690629263815598	0.0
14	1.1165474566165486E-4	0.0	0.0	20.982941387957812	0.0
15	1.1165474566165486E-4	0.0	0.0	21.614460629420133	0.0
16	1.1165474566165486E-4	0.0	0.0	22.94348706703081	1.1165474566165486E-4
17	1.1165474566165486E-4	0.0	0.0	24.506988470530963	1.1165474566165486E-4
18	1.1165474566165486E-4	0.0	0.0	26.37787538883765	1.1165474566165486E-4
19	2.2330949132330971E-4	0.0	0.0	27.291434517841314	1.1165474566165486E-4
20	2.2330949132330971E-4	0.0	0.0	28.205998539555928	1.1165474566165486E-4
21	5.582737283082743E-4	0.0	0.0	29.27553934824892	1.1165474566165486E-4
22	7.81583219631584E-4	0.0	0.0	30.38203787775592	1.1165474566165486E-4
23	0.0013398569479398583	0.0	0.0	31.405353621744986	1.1165474566165486E-4
24	0.0018981306762481326	0.0	0.0	32.237069822178654	1.1165474566165486E-4
25	0.0022330949132330974	0.0	0.0	33.01340526876414	1.1165474566165486E-4
26	0.002344749658894752	0.0	0.0	33.67853258867061	1.1165474566165486E-4
27	0.002344749658894752	0.0	0.0	34.322892125884024	1.1165474566165486E-4
28	0.002344749658894752	0.0	0.0	35.006777443061665	1.1165474566165486E-4
29	0.002344749658894752	0.0	0.0	35.6921142719329	1.1165474566165486E-4
30	0.002344749658894752	0.0	0.0	36.47883360986492	1.1165474566165486E-4
31	0.002344749658894752	0.0	0.0	37.2115120508967	1.1165474566165486E-4
32	0.002344749658894752	0.0	0.0	37.89919362942683	1.1165474566165486E-4
33	0.002344749658894752	0.0	0.0	38.539756905287746	1.1165474566165486E-4
34	0.0024564044045564067	0.0	0.0	39.166028373703966	1.1165474566165486E-4
35	0.0024564044045564067	0.0	0.0	39.84276778715926	1.1165474566165486E-4
36	0.0024564044045564067	0.0	0.0	40.49527812080597	1.1165474566165486E-4
37	0.0025680591502180617	0.0	0.0	41.11049576940169	1.1165474566165486E-4
38	0.0025680591502180617	0.0	0.0	41.751952283227894	1.1165474566165486E-4
39	0.0027913686415413715	0.0	0.0	42.37252935961537	1.1165474566165486E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGAT	20	7.0324505E-4	45.000004	27
ACGCATA	40	6.8121153E-9	45.000004	31
ATTCGGA	20	7.0324505E-4	45.000004	22
CGCGACA	20	7.0324505E-4	45.000004	35
CCCGTTG	20	7.0324505E-4	45.000004	18
TGCGCTA	20	7.0324505E-4	45.000004	2
TATTACG	20	7.0324505E-4	45.000004	1
ACGCTAT	20	7.0324505E-4	45.000004	43
TCCCGTT	20	7.0324505E-4	45.000004	17
TACTCGC	20	7.0324505E-4	45.000004	45
ATGCGTC	20	7.0324505E-4	45.000004	12
TATAGCG	20	7.0324505E-4	45.000004	1
TATACGG	40	6.8121153E-9	45.000004	2
TCGAACG	20	7.0324505E-4	45.000004	1
TAGTACG	20	7.0324505E-4	45.000004	36
CGCATCC	35	1.2115197E-7	45.0	11
ATTCGTG	25	3.8901144E-5	45.0	1
GATATAC	25	3.8901144E-5	45.0	9
TTGCGAC	25	3.8901144E-5	45.0	2
CGACGGA	30	2.164963E-6	44.999996	17
>>END_MODULE