FastQCFastQC Report
Sat 18 Jun 2016
SRR3552422_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552422_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1994755
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC61650.30906051119059735TruSeq Adapter, Index 23 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC53650.26895533536699995TruSeq Adapter, Index 23 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCT45210.2266443748731047TruSeq Adapter, Index 22 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC32400.1624259620855694TruSeq Adapter, Index 23 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC23080.11570343225107844TruSeq Adapter, Index 22 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAA253.8918406E-545.00000415
CTACGAA1600.045.00000411
CGATCTC207.0345344E-445.00000410
GTTAGCG1050.045.01
ATTAGCG900.042.5000041
GTATGCG700.041.7857131
AAGTACG603.6379788E-1241.2500041
CTACGCA556.184564E-1140.90909215
GCTACGA1800.040.00000410
TATCGTG451.929584E-840.0000041
CGTAAGG3100.039.1935462
CGTATGG1850.038.918922
ACGATAG1050.038.571431
TACGACG700.038.5714261
TACTGCG700.038.5714261
ACGTCAA356.2506024E-638.57142622
TCGTGTA1350.038.33333217
CCGTAAG1000.038.2500041
CGTTAGG3200.037.9687542
ATGGGCG6150.037.6829265
Produced by FastQC (version 0.11.5)