##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552422_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1994755
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.656641041130364	31.0	31.0	34.0	30.0	34.0
2	31.826588227626953	31.0	31.0	34.0	30.0	34.0
3	31.877229534454106	33.0	31.0	34.0	30.0	34.0
4	35.48144158054498	37.0	35.0	37.0	33.0	37.0
5	35.46937142656617	37.0	35.0	37.0	33.0	37.0
6	35.485123737000286	37.0	35.0	37.0	33.0	37.0
7	35.90611779391454	37.0	35.0	37.0	35.0	37.0
8	35.94029392080732	37.0	35.0	37.0	35.0	37.0
9	37.65680747761003	39.0	37.0	39.0	35.0	39.0
10	36.95245180485824	39.0	37.0	39.0	33.0	39.0
11	36.77192938481167	39.0	37.0	39.0	32.0	39.0
12	36.62335073730859	39.0	35.0	39.0	32.0	39.0
13	36.60849126835125	39.0	35.0	39.0	32.0	39.0
14	37.69534604500302	39.0	36.0	41.0	33.0	41.0
15	37.69701592426138	39.0	36.0	41.0	33.0	41.0
16	37.70736656882675	39.0	36.0	41.0	33.0	41.0
17	37.67908139094776	39.0	36.0	41.0	33.0	41.0
18	37.66139851761244	39.0	36.0	41.0	33.0	41.0
19	37.63759409050234	39.0	36.0	41.0	32.0	41.0
20	37.560758088086004	39.0	36.0	41.0	32.0	41.0
21	37.518298237126864	39.0	36.0	41.0	32.0	41.0
22	37.5022606786297	39.0	36.0	41.0	32.0	41.0
23	37.44550834563643	39.0	36.0	41.0	32.0	41.0
24	37.45142285644101	39.0	36.0	41.0	32.0	41.0
25	37.398602334622545	39.0	36.0	41.0	32.0	41.0
26	37.330652135224625	39.0	36.0	41.0	32.0	41.0
27	37.27626901549313	39.0	36.0	41.0	32.0	41.0
28	37.182820446621264	39.0	35.0	41.0	31.0	41.0
29	37.151711864364295	39.0	35.0	41.0	31.0	41.0
30	37.06596348925056	39.0	35.0	41.0	31.0	41.0
31	36.97541151670255	39.0	35.0	41.0	31.0	41.0
32	36.889941371246096	39.0	35.0	40.0	31.0	41.0
33	36.84519201606212	39.0	35.0	40.0	31.0	41.0
34	36.74590964805201	39.0	35.0	40.0	30.0	41.0
35	36.7336580181526	39.0	35.0	40.0	30.0	41.0
36	36.665517319169524	39.0	35.0	40.0	30.0	41.0
37	36.54268017876882	39.0	35.0	40.0	30.0	41.0
38	36.499004639667525	39.0	35.0	40.0	30.0	41.0
39	36.49304902105772	39.0	35.0	40.0	30.0	41.0
40	36.43154873656163	39.0	35.0	40.0	30.0	41.0
41	36.38332176131906	38.0	35.0	40.0	30.0	41.0
42	36.33905968402134	38.0	35.0	40.0	30.0	41.0
43	36.26691899506456	38.0	35.0	40.0	30.0	41.0
44	36.179543352441776	38.0	35.0	40.0	30.0	41.0
45	36.09306355918396	38.0	35.0	40.0	30.0	41.0
46	35.980316379705776	38.0	35.0	40.0	29.0	41.0
47	35.88251840451584	38.0	34.0	40.0	29.0	41.0
48	35.81212379465148	38.0	34.0	40.0	29.0	41.0
49	35.77342129735231	38.0	34.0	40.0	29.0	41.0
50	35.679684973843905	38.0	34.0	40.0	28.0	41.0
51	34.46749450433762	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	24.0
9	65.0
10	122.0
11	88.0
12	60.0
13	30.0
14	31.0
15	70.0
16	103.0
17	278.0
18	509.0
19	936.0
20	1537.0
21	2492.0
22	3819.0
23	5756.0
24	8522.0
25	11553.0
26	15097.0
27	19374.0
28	24472.0
29	31616.0
30	40469.0
31	52224.0
32	67065.0
33	89678.0
34	137532.0
35	162489.0
36	178549.0
37	250365.0
38	396242.0
39	493512.0
40	73.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.3235434928099	20.943825181538585	25.998029833237666	10.734601492413855
2	30.985459367190455	24.22548132477422	26.205473855185225	18.583585452850098
3	31.962621976132404	24.157101999994985	26.869415040944876	17.010860982927728
4	28.124606781283916	26.95925063478974	25.77730097179854	19.138841612127806
5	24.958303150010906	30.41215587879213	25.21758311171046	19.411957859486503
6	25.7417076282551	35.893831573300986	23.61096976821715	14.75349103022677
7	83.45561234337049	5.544690952021677	6.691247797348547	4.3084489072592875
8	84.76534712282961	4.1905898218076905	6.358274575073129	4.685788480289559
9	78.6828457630135	6.634448842088377	8.973683484939253	5.709021909958867
10	39.466400635667036	25.418159122298228	18.328967717840037	16.7864725241947
11	29.78255474983143	26.82544974194826	22.835836982486573	20.556158525733736
12	25.261147358948843	24.20242084867565	29.603284613899955	20.933147178475554
13	24.196004020543878	25.559930918834645	30.90940992753496	19.33465513308652
14	21.805484884108576	27.49059408298262	29.300490536431795	21.40343049647701
15	21.52304418337089	26.73265639138641	31.922917852067044	19.821381573175653
16	23.33018340598219	25.632120235317117	29.23196081724322	21.805735541457473
17	23.34858165539126	25.91616514308775	27.37539196542934	23.35986123609165
18	24.336572661805587	25.134615529225396	29.19511418695529	21.33369762201373
19	23.50053014029292	27.988549972302362	27.238934104689548	21.27198578271517
20	24.59109013387609	27.219432962945323	27.467282949535154	20.72219395364343
21	23.800165935164973	28.153733165225802	28.05176575569431	19.994335143914917
22	22.403854107396647	25.76165995322734	26.791761394256437	25.042724545119576
23	21.97126965466937	27.584991640577417	27.193314467190206	23.25042423756301
24	22.542868673095192	26.083704515090826	28.09141974828989	23.28200706352409
25	21.450052763371943	28.017425698895355	26.696661996084732	23.83585954164797
26	21.265669217522955	27.97486408105256	27.797198152154024	22.962268549270462
27	23.07496409333477	26.6045704860998	27.41083491456344	22.909630506001992
28	20.191502214557676	26.639562252005884	29.365711578614917	23.80322395482152
29	21.663111509934804	25.485385423272533	28.068860586889116	24.782642479903547
30	23.469298234620293	25.64029166489118	27.019107609706456	23.871302490782075
31	21.988665274682855	27.381708530621555	26.690495825301852	23.939130369393734
32	21.716501525249967	27.50839075475434	26.83377156593166	23.941336154064032
33	22.334171364403147	25.72466292853007	26.356570105100623	25.584595601966154
34	19.71630601251783	25.228010457424592	29.56047233870826	25.495211191349316
35	20.863715092830947	25.6243498575013	27.397349549192757	26.114585500474995
36	22.11309158267557	26.59118538366867	26.41863286468764	24.87709016896812
37	21.003581893515744	26.811813982168236	30.10444891728558	22.080155207030437
38	19.80498858255776	27.440613007612463	28.64452025436708	24.109878155462702
39	21.408995089622536	24.313462054237238	28.64817985166098	25.629363004479245
40	21.510561447395794	24.068670087303953	29.809224691754128	24.611543773546124
41	19.614188208576994	26.655654453805106	26.819634491453836	26.910522846164064
42	20.833185027735233	25.03034207208404	27.611811976909443	26.52466092327128
43	21.692037367997575	23.471854939579046	29.318989048780423	25.517118643642956
44	20.95711002103015	23.79490213083812	28.628478184037636	26.61950966409409
45	21.612328331048175	22.900255921153224	28.827800907880917	26.659614839917683
46	21.577336565142087	24.563718351376483	28.56125188306333	25.2976932004181
47	19.364583620544877	24.70869856197879	30.88253945973315	25.04417835774318
48	19.69088935733962	24.006657459186716	30.210426844399436	26.09202633907422
49	20.965381713543767	22.67651917152733	31.115751057147367	25.242348057781534
50	20.210000726906312	22.554599437023594	30.10053866264278	27.13486117342731
51	19.572027642492436	22.80009324453379	28.216297239510617	29.411581873463156
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	376.0
1	632.5
2	889.0
3	1690.5
4	2492.0
5	1935.5
6	1379.0
7	1451.5
8	1524.0
9	1699.0
10	1874.0
11	2097.5
12	2321.0
13	2436.0
14	2551.0
15	2754.0
16	2957.0
17	3033.5
18	3110.0
19	3403.5
20	3697.0
21	4205.5
22	4714.0
23	5111.5
24	5509.0
25	7630.5
26	12605.0
27	15458.0
28	18914.0
29	22370.0
30	27469.5
31	32569.0
32	34679.5
33	36790.0
34	42703.0
35	48616.0
36	55073.0
37	61530.0
38	66148.0
39	70766.0
40	79831.0
41	88896.0
42	98532.0
43	108168.0
44	122184.0
45	136200.0
46	159053.0
47	181906.0
48	206604.0
49	231302.0
50	226289.5
51	221277.0
52	194286.0
53	167295.0
54	146247.0
55	125199.0
56	110170.5
57	95142.0
58	85535.5
59	75929.0
60	71157.0
61	66385.0
62	59341.5
63	52298.0
64	45152.0
65	38006.0
66	32165.0
67	26324.0
68	22424.0
69	18524.0
70	14821.0
71	11118.0
72	9551.0
73	7984.0
74	6725.5
75	4161.0
76	2855.0
77	2166.0
78	1477.0
79	1269.5
80	1062.0
81	731.5
82	401.0
83	279.0
84	157.0
85	130.5
86	104.0
87	62.0
88	20.0
89	12.0
90	4.0
91	4.5
92	5.0
93	5.5
94	6.0
95	3.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1994755.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.405254960939804
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.85478396454796	16.730575770169516
2	13.57751232794952	6.898803248537219
3	5.955960819045325	4.539381094356431
4	3.021011509072008	3.069982705116316
5	1.8761267411025782	2.3831739098374047
6	1.1747215257800734	1.7906459922328195
7	0.8089221131523404	1.4385610769724186
8	0.6050025474118534	1.229619517521297
9	0.46682237479139427	1.0673767307742095
>10	5.283704576594085	33.10915561088715
>50	1.2678832463872702	21.05287139621617
>100	0.10080172923897211	4.408707636368698
>500	0.0041669712782245955	0.7076183229244005
>1k	0.0021826992409747885	0.9922888842661466
>5k	3.9685440744996144E-4	0.5812381038197861
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC	6165	0.30906051119059735	TruSeq Adapter, Index 23 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC	5365	0.26895533536699995	TruSeq Adapter, Index 23 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCT	4521	0.2266443748731047	TruSeq Adapter, Index 22 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC	3240	0.1624259620855694	TruSeq Adapter, Index 23 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC	2308	0.11570343225107844	TruSeq Adapter, Index 22 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.24589485926843146	0.0
2	0.0	0.0	0.0	1.1791423006835426	0.0
3	0.0	0.0	0.0	1.672185305964893	0.0
4	0.0	0.0	0.0	2.252607463071906	0.0
5	0.0	0.0	0.0	3.762116149602332	0.0
6	0.0	0.0	0.0	4.632398464974395	0.0
7	0.0	0.0	0.0	5.4928048807998975	0.0
8	0.0	0.0	0.0	6.943760010627871	0.0
9	0.0	0.0	0.0	7.4449243140135	0.0
10	0.0	0.0	0.0	8.794212823128655	0.0
11	0.0	0.0	0.0	10.757712099982204	0.0
12	0.0	0.0	0.0	12.153923664810966	0.0
13	0.0	0.0	0.0	12.695092881080633	0.0
14	0.0	0.0	0.0	12.905494659745182	0.0
15	0.0	0.0	0.0	13.31802652456066	0.0
16	0.0	0.0	0.0	14.26450867399756	0.0
17	0.0	0.0	0.0	15.474882880353727	0.0
18	0.0	0.0	0.0	16.721652533769813	0.0
19	0.0	0.0	0.0	17.505407932302465	0.0
20	0.0	0.0	0.0	18.245950003885188	0.0
21	0.0	0.0	0.0	19.180801652333244	0.0
22	1.503944093384902E-4	0.0	0.0	20.18548643818414	0.0
23	2.005258791179869E-4	0.0	0.0	21.165757198252418	0.0
24	3.5092028845647714E-4	0.0	0.0	21.961794806881045	0.0
25	4.010517582359738E-4	0.0	0.0	22.67356141481034	0.0
26	4.010517582359738E-4	0.0	0.0	23.32115974142188	0.0
27	4.010517582359738E-4	0.0	0.0	23.96454702457194	0.0
28	4.010517582359738E-4	0.0	0.0	24.60773378184288	0.0
29	4.010517582359738E-4	0.0	0.0	25.2813002098002	0.0
30	4.010517582359738E-4	0.0	0.0	26.019987417001087	0.0
31	4.010517582359738E-4	0.0	0.0	26.74168005594672	0.0
32	4.010517582359738E-4	0.0	0.0	27.432541840977965	0.0
33	4.010517582359738E-4	0.0	0.0	28.111522467671467	0.0
34	4.5118322801547057E-4	0.0	0.0	28.752302914392995	0.0
35	4.5118322801547057E-4	0.0	0.0	29.43815155244629	0.0
36	4.5118322801547057E-4	0.0	0.0	30.14650922043058	0.0
37	4.5118322801547057E-4	0.0	0.0	30.827444974445484	0.0
38	5.51446167574464E-4	0.0	0.0	31.54206907615221	0.0
39	5.51446167574464E-4	0.0	0.0	32.23964847813391	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGCTAA	25	3.8918406E-5	45.000004	15
CTACGAA	160	0.0	45.000004	11
CGATCTC	20	7.0345344E-4	45.000004	10
GTTAGCG	105	0.0	45.0	1
ATTAGCG	90	0.0	42.500004	1
GTATGCG	70	0.0	41.785713	1
AAGTACG	60	3.6379788E-12	41.250004	1
CTACGCA	55	6.184564E-11	40.909092	15
GCTACGA	180	0.0	40.000004	10
TATCGTG	45	1.929584E-8	40.000004	1
CGTAAGG	310	0.0	39.193546	2
CGTATGG	185	0.0	38.91892	2
ACGATAG	105	0.0	38.57143	1
TACGACG	70	0.0	38.571426	1
TACTGCG	70	0.0	38.571426	1
ACGTCAA	35	6.2506024E-6	38.571426	22
TCGTGTA	135	0.0	38.333332	17
CCGTAAG	100	0.0	38.250004	1
CGTTAGG	320	0.0	37.968754	2
ATGGGCG	615	0.0	37.682926	5
>>END_MODULE