FastQCFastQC Report
Sat 18 Jun 2016
SRR3552420_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552420_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences895195
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC53600.5987522271683823No Hit
CTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCT53550.5981936896430387No Hit
CCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC47070.5258072263585029No Hit
TCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC27740.3098766190606516No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA19300.21559548478264512No Hit
ACTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC17780.19861594401219848No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14480.1617524673395182No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG13630.1522573294086763No Hit
GCCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTG11720.13092119594054927No Hit
CGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTG10940.12220801054518848No Hit
ACCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTG10460.11684605030188952No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCC10000.11170750506872805No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG207.0324494E-445.01
CAAACGG253.890112E-545.02
TAGGTCG253.890112E-545.01
TAACGCG351.2115197E-745.01
CATAGTA207.0324494E-445.041
ACAATCG253.890112E-545.02
CGTACTT207.0324494E-445.028
ACTCGAA207.0324494E-445.022
ACGTACT207.0324494E-445.027
GATCTCG351.2115197E-745.010
ATACGCG207.0324494E-445.01
TCGAATC207.0324494E-445.024
GTAGTAC207.0324494E-445.036
ATCGTAG302.1649612E-644.9999961
CGAATAT1050.042.85714314
ACAATAG850.042.35294311
AGTACGG750.042.02
GTTAGCG700.041.7857131
ATTGGGT556.002665E-1140.9090924
ATAGTGT1100.040.90909211