##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552420_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	895195
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.471365456688208	31.0	31.0	34.0	30.0	34.0
2	31.627093538279368	31.0	31.0	34.0	30.0	34.0
3	31.681778830310716	31.0	31.0	34.0	30.0	34.0
4	35.32225939599752	37.0	35.0	37.0	33.0	37.0
5	35.3191472249063	37.0	35.0	37.0	33.0	37.0
6	35.33818553499517	37.0	35.0	37.0	33.0	37.0
7	35.76504448751389	37.0	35.0	37.0	35.0	37.0
8	35.777906489647506	37.0	35.0	37.0	35.0	37.0
9	37.50234529906892	39.0	37.0	39.0	35.0	39.0
10	36.91873167298745	39.0	37.0	39.0	33.0	39.0
11	36.70892487111747	39.0	35.0	39.0	32.0	39.0
12	36.48403420483805	38.0	35.0	39.0	32.0	39.0
13	36.38583883958244	38.0	35.0	39.0	32.0	39.0
14	37.408166935695576	39.0	36.0	41.0	32.0	41.0
15	37.45037114818559	39.0	36.0	41.0	32.0	41.0
16	37.47835722943046	39.0	36.0	41.0	32.0	41.0
17	37.463831902546374	39.0	36.0	41.0	32.0	41.0
18	37.418037410843446	39.0	36.0	41.0	32.0	41.0
19	37.38737146655198	39.0	36.0	41.0	32.0	41.0
20	37.287990884667586	39.0	35.0	41.0	32.0	41.0
21	37.21854009461626	39.0	35.0	40.0	32.0	41.0
22	37.19637955976072	39.0	35.0	40.0	32.0	41.0
23	37.1488066845771	39.0	35.0	40.0	32.0	41.0
24	37.14737459436212	39.0	35.0	41.0	32.0	41.0
25	37.057857785175294	39.0	35.0	40.0	31.0	41.0
26	36.967449550098024	39.0	35.0	40.0	31.0	41.0
27	36.87828461955216	39.0	35.0	40.0	31.0	41.0
28	36.81480682979686	39.0	35.0	40.0	31.0	41.0
29	36.75370282452427	39.0	35.0	40.0	31.0	41.0
30	36.61679857461223	39.0	35.0	40.0	31.0	41.0
31	36.47202453096811	38.0	35.0	40.0	30.0	41.0
32	36.36176140393992	38.0	35.0	40.0	30.0	41.0
33	36.16682398806964	38.0	35.0	40.0	30.0	41.0
34	36.006897938437994	38.0	35.0	40.0	30.0	41.0
35	35.978603544479135	38.0	35.0	40.0	30.0	41.0
36	35.83611838761387	38.0	35.0	40.0	29.0	41.0
37	35.65847999597853	38.0	34.0	40.0	28.0	41.0
38	35.605365311468454	38.0	34.0	40.0	28.0	41.0
39	35.52429135551472	38.0	34.0	40.0	27.0	41.0
40	35.38391300219505	38.0	34.0	40.0	27.0	41.0
41	35.35430269382648	38.0	34.0	40.0	27.0	41.0
42	35.24373907360966	38.0	34.0	40.0	27.0	41.0
43	35.063898927049415	38.0	34.0	40.0	26.0	41.0
44	34.91723702656963	38.0	34.0	40.0	26.0	41.0
45	34.856827841978564	37.0	33.0	40.0	26.0	41.0
46	34.698589692748506	37.0	33.0	40.0	25.0	41.0
47	34.55054932165618	37.0	33.0	40.0	25.0	41.0
48	34.48496472835527	37.0	33.0	40.0	25.0	41.0
49	34.41428292159809	36.0	33.0	40.0	25.0	41.0
50	34.222179525131395	36.0	33.0	40.0	24.0	41.0
51	32.87496802372667	35.0	31.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	8.0
9	45.0
10	64.0
11	48.0
12	29.0
13	17.0
14	22.0
15	39.0
16	102.0
17	191.0
18	468.0
19	824.0
20	1275.0
21	2028.0
22	3038.0
23	4252.0
24	6270.0
25	8324.0
26	9892.0
27	12030.0
28	14594.0
29	17427.0
30	21810.0
31	27258.0
32	34197.0
33	45007.0
34	68999.0
35	79499.0
36	82921.0
37	113565.0
38	168082.0
39	172842.0
40	27.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.900150246594315	19.91130424097543	25.37223733376527	11.816308178664983
2	30.85707583263982	24.31995263601785	26.077893643284423	18.74507788805791
3	32.305363635855876	24.78543780963924	26.233278782835022	16.675919771669857
4	29.187048631862332	26.890230620144212	25.48025849116673	18.442462256826726
5	24.929875613693106	29.658454303252363	25.075653907807794	20.336016175246733
6	25.098442238841816	34.990030105172615	24.29202575975067	15.6195018962349
7	77.98971173878317	7.132747613648423	9.09243237506912	5.785108272499287
8	77.22518557409279	6.323650154435626	9.374717240377795	7.076447031093784
9	71.95884695513269	8.334161830662593	12.23107814498517	7.475913069219556
10	40.17828517808969	24.232597367054108	19.15951273186289	16.429604722993314
11	29.986315830629078	25.718865721993534	23.09921302062679	21.195605426750596
12	26.366992666402293	22.56201162875128	30.424432665508633	20.6465630393378
13	23.601896793436065	24.98930400638967	31.456610012343678	19.952189187830584
14	18.951289942414782	28.24300850652651	30.46330687727255	22.34239467378616
15	18.736141287652412	25.332022631940525	34.35307391127073	21.578762169136333
16	20.00625562028385	25.812588318746194	30.902652494707855	23.2785035662621
17	19.970397511156786	24.67071420193366	28.755634247286903	26.603254039622655
18	20.85433899876563	24.61072727171175	31.705270918626667	22.82966281089595
19	22.43890995816554	26.855601293572906	28.210166500036305	22.495322248225246
20	24.101676171113557	27.496355542647134	27.64582018442909	20.756148101810222
21	22.076419104217518	28.48999380023347	28.343656968593432	21.08993012695558
22	19.832438742396906	26.44362401487944	26.471215768631417	27.25272147409224
23	20.188450561050946	27.402968068409677	27.525064371449798	24.883516999089583
24	22.28441847865549	25.228804897257024	27.94519629801328	24.541580326074207
25	20.447388557800256	27.68447098118287	26.20747434916415	25.660666111852727
26	20.166555890057474	28.325336937762163	27.755516954406584	23.75259021777378
27	22.01352777886382	29.014348829026076	27.066281648132527	21.905841743977568
28	18.227983847094766	28.779874775886817	28.835169990895835	24.156971386122578
29	21.785644468523618	26.447645485061916	27.624930880981243	24.14177916543323
30	21.994872625517345	28.3780628801546	25.405973000296022	24.221091494032027
31	21.61171588313161	32.09903987399393	22.22889984863633	24.060344394238125
32	23.00347968878289	28.64180429962187	23.73158920682086	24.62312680477438
33	21.962365741542346	28.091756544663454	22.990186495679712	26.95569121811449
34	20.48279983690704	27.70558369964086	26.122911767827123	25.688704695624974
35	22.26576332530901	25.30107965303649	25.21506487413357	27.218092147520935
36	23.5060517540871	30.240003574640163	23.33189975368495	22.92204491758779
37	21.02759733912723	30.619250554348493	26.897826730488887	21.45532537603539
38	19.62846083814141	28.93939309312496	25.444512089544734	25.98763397918889
39	23.293695786951446	24.1636738364267	26.819519769435708	25.723110607186143
40	23.290344561799383	23.913002195052474	27.01344399823502	25.78320924491312
41	21.45722440362156	25.845095202721197	23.96237691229285	28.735303481364394
42	24.957020537424807	24.836153016940443	25.4389267142913	24.76789973134345
43	24.519797362585805	23.689140354894743	27.90330598361251	23.887756298906943
44	21.286982165896813	24.737180167449548	26.925641899251	27.050195767402634
45	22.391992806036672	22.697512832399642	27.174079390523854	27.73641497103983
46	23.07564273705729	24.372008333379878	26.752160143879266	25.800188785683563
47	19.722965387429557	25.720541334569564	29.492680365730372	25.06381291227051
48	20.454649545629724	23.30911142265093	29.29797418439558	26.93826484732377
49	23.431654555711326	21.757605884751367	30.058702293913615	24.75203726562369
50	20.950072330609533	21.339931523299395	29.471455939767317	28.238540206323766
51	20.011617580527147	21.184546383748792	26.085154631113895	32.718681404610166
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	496.0
1	723.0
2	950.0
3	1943.0
4	2936.0
5	2233.5
6	1531.0
7	1663.0
8	1795.0
9	1863.5
10	1932.0
11	2173.5
12	2415.0
13	2509.0
14	2603.0
15	2595.5
16	2588.0
17	2582.5
18	2577.0
19	2504.5
20	2432.0
21	2596.5
22	2761.0
23	2841.0
24	2921.0
25	4717.0
26	6552.5
27	6592.0
28	7625.5
29	8659.0
30	9908.0
31	11157.0
32	12638.0
33	14119.0
34	15952.0
35	17785.0
36	18733.0
37	19681.0
38	20431.5
39	21182.0
40	25338.5
41	29495.0
42	35989.0
43	42483.0
44	50046.5
45	57610.0
46	70606.5
47	83603.0
48	100710.5
49	117818.0
50	115319.5
51	112821.0
52	95329.5
53	77838.0
54	65596.0
55	53354.0
56	45910.5
57	38467.0
58	35418.0
59	32369.0
60	31646.5
61	30924.0
62	27292.0
63	23660.0
64	20610.5
65	17561.0
66	14848.0
67	12135.0
68	10698.0
69	9261.0
70	8334.0
71	7407.0
72	6361.5
73	5316.0
74	4554.5
75	3103.0
76	2413.0
77	1919.5
78	1426.0
79	1135.0
80	844.0
81	652.5
82	461.0
83	381.5
84	302.0
85	205.0
86	108.0
87	82.0
88	56.0
89	40.5
90	25.0
91	15.5
92	6.0
93	9.5
94	13.0
95	7.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	895195.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.081882448705425
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.82892535921994	19.43510951470143
2	12.746513457455672	7.413852120011352
3	5.8321151391166115	5.0882666070931375
4	3.1932716065610247	3.714653979551858
5	1.9691733072813602	2.8633633321742504
6	1.3346528702774894	2.3288530729942343
7	0.9221708670496152	1.8772925327210366
8	0.6769779986214887	1.5750235661016
9	0.5369475528064711	1.405390105065406
>10	5.333971977720165	35.308782584318784
>50	0.5164519489861343	9.596196563408764
>100	0.09921414150260263	5.211942083563635
>500	0.005383713104792315	1.0468841696901294
>1k	0.0034609584245093453	1.9360814812173401
>5k	7.691018721131878E-4	1.1983082873870032
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC	5360	0.5987522271683823	No Hit
CTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCT	5355	0.5981936896430387	No Hit
CCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC	4707	0.5258072263585029	No Hit
TCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC	2774	0.3098766190606516	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1930	0.21559548478264512	No Hit
ACTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC	1778	0.19861594401219848	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1448	0.1617524673395182	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1363	0.1522573294086763	No Hit
GCCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTG	1172	0.13092119594054927	No Hit
CGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTG	1094	0.12220801054518848	No Hit
ACCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTG	1046	0.11684605030188952	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCC	1000	0.11170750506872805	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.234150101374561E-4	0.0	0.0	0.6648830701690693	0.0
2	2.234150101374561E-4	0.0	0.0	2.485938817799474	0.0
3	2.234150101374561E-4	0.0	0.0	3.315925580460123	0.0
4	2.234150101374561E-4	0.0	0.0	4.209250498494741	0.0
5	2.234150101374561E-4	0.0	0.0	6.5491876071693875	0.0
6	2.234150101374561E-4	0.0	0.0	7.801875569010104	0.0
7	2.234150101374561E-4	0.0	0.0	9.183585699205201	0.0
8	2.234150101374561E-4	0.0	0.0	11.408352370153988	0.0
9	2.234150101374561E-4	0.0	0.0	12.177905372572456	0.0
10	2.234150101374561E-4	0.0	0.0	14.331290947782326	0.0
11	2.234150101374561E-4	0.0	0.0	17.512608984634632	0.0
12	2.234150101374561E-4	0.0	0.0	19.970620926166923	0.0
13	2.234150101374561E-4	0.0	0.0	20.968615776450942	0.0
14	2.234150101374561E-4	0.0	0.0	21.345070068532554	0.0
15	2.234150101374561E-4	0.0	0.0	22.03207122470523	0.0
16	2.234150101374561E-4	0.0	0.0	23.478124877819916	0.0
17	2.234150101374561E-4	0.0	0.0	25.232156122409084	0.0
18	2.234150101374561E-4	0.0	0.0	27.38487145258854	0.0
19	2.234150101374561E-4	0.0	0.0	28.430453700031837	0.0
20	2.234150101374561E-4	0.0	0.0	29.407335831857864	0.0
21	0.0012287825557560084	0.0	0.0	30.555912398974524	0.0
22	0.0015639050709621927	0.0	0.0	31.706387993677357	0.0
23	0.002122442596305833	0.0	0.0	32.84647479040879	0.0
24	0.002680980121649473	0.0	0.0	33.78671686057228	0.0
25	0.0059204977686425865	0.0	0.0	34.594808952239454	0.0
26	0.0059204977686425865	0.0	0.0	35.3180033400544	0.0
27	0.006143912778780043	0.0	0.0	36.008132306369006	0.0
28	0.006143912778780043	0.0	0.0	36.70675104306883	0.0
29	0.006143912778780043	0.0	0.0	37.425477130681024	0.0
30	0.006143912778780043	0.0	0.0	38.23759069253068	0.0
31	0.006143912778780043	0.0	0.0	39.01071833511134	0.0
32	0.006143912778780043	0.0	0.0	39.74519518093823	0.0
33	0.006143912778780043	0.0	0.0	40.49698669005077	0.0
34	0.006925865314261139	0.0	0.0	41.217723512754205	0.0
35	0.007149280324398595	0.0	0.0	41.92416177480884	0.0
36	0.007149280324398595	0.0	0.0	42.644675182502134	0.0
37	0.007149280324398595	0.0	0.0	43.31793631555136	0.0
38	0.007260987829467323	0.0	0.0	44.01052284697747	0.0
39	0.007484402839604779	0.0	0.0	44.66445858164981	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	20	7.0324494E-4	45.0	1
CAAACGG	25	3.890112E-5	45.0	2
TAGGTCG	25	3.890112E-5	45.0	1
TAACGCG	35	1.2115197E-7	45.0	1
CATAGTA	20	7.0324494E-4	45.0	41
ACAATCG	25	3.890112E-5	45.0	2
CGTACTT	20	7.0324494E-4	45.0	28
ACTCGAA	20	7.0324494E-4	45.0	22
ACGTACT	20	7.0324494E-4	45.0	27
GATCTCG	35	1.2115197E-7	45.0	10
ATACGCG	20	7.0324494E-4	45.0	1
TCGAATC	20	7.0324494E-4	45.0	24
GTAGTAC	20	7.0324494E-4	45.0	36
ATCGTAG	30	2.1649612E-6	44.999996	1
CGAATAT	105	0.0	42.857143	14
ACAATAG	85	0.0	42.352943	11
AGTACGG	75	0.0	42.0	2
GTTAGCG	70	0.0	41.785713	1
ATTGGGT	55	6.002665E-11	40.909092	4
ATAGTGT	110	0.0	40.909092	11
>>END_MODULE