FastQCFastQC Report
Sat 18 Jun 2016
SRR3552418_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552418_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1162702
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC95270.8193845026498621TruSeq Adapter, Index 15 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC85390.7344100207963863TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCT73890.6355024761288791TruSeq Adapter, Index 14 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC49800.4283126716905966TruSeq Adapter, Index 15 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC31760.2731568364034809TruSeq Adapter, Index 14 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG18270.15713398618046584No Hit
AGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG15490.13322416233910322TruSeq Adapter, Index 14 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG15130.13012792615820734TruSeq Adapter, Index 14 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG15110.12995591303704646TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCC13900.1195491192068131No Hit
GGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG13350.11481875837488884No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACCG207.0333196E-445.0000048
GCGTTGT207.0333196E-445.00000440
CTGCGCG207.0333196E-445.0000041
CCCGTTA207.0333196E-445.00000414
TCGTGCG207.0333196E-445.0000041
CTACGGA207.0333196E-445.0000043
ACCGTTG207.0333196E-445.00000418
TCGAGAG207.0333196E-445.0000041
CGTCATA207.0333196E-445.00000438
TCGGTCG207.0333196E-445.00000416
CCGCACG207.0333196E-445.0000041
ATCGACC207.0333196E-445.00000410
AGTCACG253.8908336E-545.01
CGAAACG253.8908336E-545.03
ATTAACG253.8908336E-545.01
ACTACGG351.211938E-745.02
TATCGAG351.211938E-745.01
TCGAAAC253.8908336E-545.02
ACGCGAG302.1655233E-644.9999961
GATCGAC302.1655233E-644.9999969
Produced by FastQC (version 0.11.5)