##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552418_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1162702
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.461748582181848	31.0	31.0	34.0	30.0	34.0
2	31.61877936048962	31.0	31.0	34.0	30.0	34.0
3	31.661725876449857	31.0	31.0	34.0	30.0	34.0
4	35.31349133311889	37.0	35.0	37.0	33.0	37.0
5	35.32385856393125	37.0	35.0	37.0	33.0	37.0
6	35.34910664985525	37.0	35.0	37.0	33.0	37.0
7	35.82813050979529	37.0	35.0	37.0	35.0	37.0
8	35.86787930183314	37.0	35.0	37.0	35.0	37.0
9	37.593219930816325	39.0	37.0	39.0	35.0	39.0
10	36.856283897335686	39.0	37.0	39.0	32.0	39.0
11	36.68267707460725	39.0	35.0	39.0	32.0	39.0
12	36.626272252047386	39.0	35.0	39.0	32.0	39.0
13	36.593575997977126	39.0	35.0	39.0	32.0	39.0
14	37.614351742750934	39.0	36.0	41.0	33.0	41.0
15	37.60887312484196	39.0	36.0	41.0	32.0	41.0
16	37.636554336364775	39.0	36.0	41.0	33.0	41.0
17	37.64094583134801	39.0	36.0	41.0	33.0	41.0
18	37.61874151760296	39.0	36.0	41.0	33.0	41.0
19	37.61845941608426	39.0	36.0	41.0	32.0	41.0
20	37.531741581247815	39.0	36.0	41.0	32.0	41.0
21	37.47677306824965	39.0	36.0	41.0	32.0	41.0
22	37.470264091744916	39.0	36.0	40.0	32.0	41.0
23	37.433029271472826	39.0	36.0	40.0	32.0	41.0
24	37.428732383706226	39.0	36.0	41.0	32.0	41.0
25	37.34263293604036	39.0	36.0	41.0	32.0	41.0
26	37.211048058745924	39.0	36.0	40.0	32.0	41.0
27	37.186138838670615	39.0	36.0	40.0	32.0	41.0
28	37.154447141227934	39.0	36.0	40.0	31.0	41.0
29	37.04909168471371	39.0	36.0	40.0	31.0	41.0
30	36.9938066675726	39.0	36.0	40.0	31.0	41.0
31	36.91726942931207	39.0	35.0	40.0	31.0	41.0
32	36.72761292231372	39.0	35.0	40.0	30.0	41.0
33	36.733196468226595	39.0	35.0	40.0	30.0	41.0
34	36.62193580126292	39.0	35.0	40.0	30.0	41.0
35	36.44751191620897	39.0	35.0	40.0	30.0	41.0
36	36.4017744873579	39.0	35.0	40.0	30.0	41.0
37	36.359683736675436	38.0	35.0	40.0	30.0	41.0
38	36.34689886144515	38.0	35.0	40.0	30.0	41.0
39	36.37421626521671	39.0	35.0	40.0	30.0	41.0
40	36.19639168075741	38.0	35.0	40.0	30.0	41.0
41	36.068830190366924	38.0	35.0	40.0	30.0	41.0
42	36.008508629038225	38.0	35.0	40.0	30.0	41.0
43	35.97923801627588	38.0	35.0	40.0	29.0	41.0
44	35.94663206909423	38.0	35.0	40.0	29.0	41.0
45	35.90259843020826	38.0	35.0	40.0	29.0	41.0
46	35.71151507436987	38.0	34.0	40.0	28.0	41.0
47	35.56437419046325	38.0	34.0	40.0	28.0	41.0
48	35.490415428888916	38.0	34.0	40.0	27.0	41.0
49	35.46690811575107	38.0	34.0	40.0	27.0	41.0
50	35.347748606263686	38.0	34.0	40.0	27.0	41.0
51	34.017591781901125	37.0	32.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	10.0
9	43.0
10	46.0
11	54.0
12	27.0
13	13.0
14	17.0
15	23.0
16	62.0
17	137.0
18	298.0
19	514.0
20	869.0
21	1528.0
22	2293.0
23	3691.0
24	5457.0
25	7483.0
26	9651.0
27	11989.0
28	15334.0
29	19744.0
30	25414.0
31	32600.0
32	41565.0
33	54693.0
34	78978.0
35	96101.0
36	108716.0
37	151376.0
38	241719.0
39	252225.0
40	30.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.85165416417965	21.824680786650404	26.224690419385187	11.09897462978476
2	29.903019002289493	24.79096105450924	26.692738122063954	18.613281821137317
3	28.976556331716985	25.15373672703754	28.695830917982423	17.173876023263055
4	27.508338336048272	27.232085263463894	26.82897251402337	18.430603886464457
5	22.94268006763556	30.193635170490808	26.620062578373478	20.243622183500158
6	23.9806932472809	33.98093406565052	26.30803077658764	15.730341910480933
7	79.17987584092914	5.45582617042028	9.555414887047583	5.80888310160299
8	78.29314820134479	4.4951328887367525	9.514733783893035	7.696985126025413
9	72.91593202729504	7.110506389427386	12.525823469814275	7.447738113463295
10	34.856652865480584	27.776248772256345	21.803609179308197	15.563489182954877
11	24.10299457642629	26.186675519608638	28.26829230533705	21.442037598628023
12	21.383209111191	22.768860808702488	35.15457959133123	20.693350488775284
13	21.881101090391176	26.313277176783046	34.51942114144467	17.28620059138111
14	19.348207881297185	29.5375771263832	30.193291144248484	20.92092384807113
15	17.248787737528616	28.15106536326591	35.069089070114266	19.531057829091203
16	18.420369105755388	27.84625811256883	31.185462827104455	22.547909954571335
17	18.958340142186046	27.921427846516135	28.218064473958073	24.902167537339746
18	19.00564375050529	26.44349110950183	33.39273519784089	21.158129942151987
19	18.96135037180636	28.19363861075323	30.324193129451913	22.520817887988496
20	20.383382844443375	28.40633283506866	30.706664304353136	20.50362001613483
21	19.80971908537183	29.44365796222936	31.13050463489355	19.61611831750526
22	17.95300945556127	27.65351741030806	28.091548823344244	26.30192431078643
23	17.242423252045665	28.413385373036252	28.92323226415711	25.420959110760972
24	20.063266425962972	26.267865712796574	29.839546160581133	23.829321700659325
25	16.822797242973696	29.820366697571693	27.713205963350884	25.64363009610373
26	16.949742926390424	30.4812411090718	29.2952966452281	23.273719319309677
27	19.61087191730985	27.712001871502757	27.631671743920627	25.045454467266765
28	15.670653357438106	27.335293136160427	29.544199631547897	27.449853874853574
29	17.105070774798705	26.280938710004797	29.903105008850073	26.710885506346425
30	19.589886316528222	26.58720807223175	26.186073473684573	27.63683213755545
31	19.207673161308744	27.845656066644764	24.953599460566853	27.99307131147964
32	17.543962253440693	28.15106536326591	29.064885069433092	25.2400873138603
33	17.64691210645548	24.521072467407816	28.566649063990603	29.265366362146107
34	18.46784472719579	24.13430096447757	30.147019614656205	27.250834693670434
35	16.89745093755752	24.434635874024472	30.457417291791018	28.210495896627
36	19.065074283866373	24.692483542644634	29.10677026443577	27.135671909053222
37	18.423723361618023	23.664447124026623	32.746567908200035	25.16526160615532
38	16.448582697888195	25.124064463637286	31.753708172859422	26.673644665615093
39	21.005296284000543	21.645873147203666	32.14185578075895	25.206974788036828
40	22.695411205966792	22.169481088017392	30.97104847157741	24.164059234438405
41	18.87095747663632	24.23922896838571	29.542393493775705	27.34742006120227
42	19.18943977046569	22.721299180701504	32.575414852644954	25.513846196187846
43	19.944319352680225	21.645529120961346	32.205586642149065	26.20456488420937
44	18.490464452628448	22.198551305493584	29.02420396627855	30.286780275599423
45	20.50138384555974	20.945177698154815	28.621865275883245	29.9315731804022
46	22.251531346811134	21.8040392121111	30.00407671097151	25.94035273010625
47	17.46801846044816	22.339688071406087	34.580829825699105	25.611463642446648
48	17.42983154755045	20.608290000361226	33.6552272207324	28.30665123135593
49	19.91851738450609	18.755880698579688	35.358501146467454	25.96710077044677
50	19.783229064713055	18.106273146515615	33.0395922600976	29.07090552867373
51	18.23579902674976	18.519362656983475	29.198969297378003	34.045869018888766
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	427.0
1	553.5
2	680.0
3	1374.5
4	2069.0
5	1566.0
6	1063.0
7	1145.0
8	1227.0
9	1365.0
10	1503.0
11	1722.0
12	1941.0
13	2085.5
14	2230.0
15	2390.0
16	2550.0
17	2661.0
18	2772.0
19	3002.0
20	3232.0
21	3422.5
22	3613.0
23	4206.5
24	4800.0
25	6229.5
26	8228.5
27	8798.0
28	10015.5
29	11233.0
30	13230.5
31	15228.0
32	17854.0
33	20480.0
34	23241.5
35	26003.0
36	29241.0
37	32479.0
38	36999.5
39	41520.0
40	46160.5
41	50801.0
42	59928.0
43	69055.0
44	81501.0
45	93947.0
46	114342.5
47	134738.0
48	159189.5
49	183641.0
50	174914.0
51	166187.0
52	134168.0
53	102149.0
54	82024.0
55	61899.0
56	50474.0
57	39049.0
58	32245.5
59	25442.0
60	20896.5
61	16351.0
62	13906.0
63	11461.0
64	9681.0
65	7901.0
66	5872.0
67	3843.0
68	2998.0
69	2153.0
70	1613.0
71	1073.0
72	898.0
73	723.0
74	507.5
75	224.0
76	156.0
77	186.5
78	217.0
79	134.0
80	51.0
81	37.0
82	23.0
83	19.0
84	15.0
85	14.0
86	13.0
87	9.5
88	6.0
89	4.5
90	3.0
91	2.0
92	1.0
93	2.5
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1162702.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.926230189209207
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.21752232500614	19.217883552788155
2	14.214036588275395	8.507450617171425
3	6.507769584337529	5.842590317976577
4	3.5965037047381103	4.3051919097734554
5	2.1319730557120593	3.190095821121542
6	1.4449809457630685	2.5945699441156083
7	1.0155382407487104	2.1273861811013326
8	0.7477326121597336	1.7901454617176706
9	0.5700312782540282	1.5352998523271288
>10	5.071139898439908	33.401975563024116
>50	0.38437306704084906	7.221971898575487
>100	0.08829912626609987	5.142779787151185
>500	0.006636862426982316	1.3450639408473102
>1k	0.002597033123601776	1.579431142661614
>5k	8.656777078672587E-4	2.1981640096474244
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	9527	0.8193845026498621	TruSeq Adapter, Index 15 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	8539	0.7344100207963863	TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCT	7389	0.6355024761288791	TruSeq Adapter, Index 14 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	4980	0.4283126716905966	TruSeq Adapter, Index 15 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	3176	0.2731568364034809	TruSeq Adapter, Index 14 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG	1827	0.15713398618046584	No Hit
AGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG	1549	0.13322416233910322	TruSeq Adapter, Index 14 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG	1513	0.13012792615820734	TruSeq Adapter, Index 14 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG	1511	0.12995591303704646	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCC	1390	0.1195491192068131	No Hit
GGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG	1335	0.11481875837488884	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7184988070890047	0.0
2	0.0	0.0	0.0	3.26025069192278	0.0
3	0.0	0.0	0.0	4.3901188782680345	0.0
4	0.0	0.0	0.0	5.631967606489023	0.0
5	0.0	0.0	0.0	8.707992245648498	0.0
6	0.0	0.0	0.0	10.31493882353346	0.0
7	0.0	0.0	0.0	12.010902191619177	0.0
8	0.0	0.0	0.0	14.670397057887575	0.0
9	0.0	0.0	0.0	15.589033131447266	0.0
10	0.0	0.0	0.0	17.94139856988291	0.0
11	0.0	0.0	0.0	21.32257448598179	0.0
12	0.0	0.0	0.0	23.825193385751465	0.0
13	0.0	0.0	0.0	24.79121907419098	0.0
14	0.0	0.0	0.0	25.139459637981187	0.0
15	0.0	0.0	0.0	25.828372188230517	0.0
16	0.0	0.0	0.0	27.467657232893725	0.0
17	0.0	0.0	0.0	29.50816288266469	0.0
18	0.0	0.0	0.0	31.808150325706844	0.0
19	0.0	0.0	0.0	33.08792794714381	0.0
20	0.0	0.0	0.0	34.19208017187551	0.0
21	4.300328029022054E-4	0.0	0.0	35.635442271536476	0.0
22	5.160393634826465E-4	0.0	0.0	37.11131485109684	0.0
23	6.880524846435286E-4	0.0	0.0	38.60318465092517	0.0
24	0.001032078726965293	0.0	0.0	39.75894081200514	0.0
25	0.0014621115298674983	0.0	0.0	40.7423398256819	0.0
26	0.0014621115298674983	0.0	0.0	41.66252401733204	0.0
27	0.0014621115298674983	0.0	0.0	42.523707708424	0.0
28	0.0014621115298674983	0.0	0.0	43.42927078477546	0.0
29	0.0014621115298674983	0.0	0.0	44.33036151997674	0.0
30	0.0014621115298674983	0.0	0.0	45.30154760205109	0.0
31	0.0016341246510283805	0.0	0.0	46.20126223228308	0.0
32	0.0016341246510283805	0.0	0.0	47.043008440683856	0.0
33	0.0018921443327697036	0.0	0.0	47.85731855625947	0.0
34	0.001978150893350145	0.0	0.0	48.6539973269161	0.0
35	0.001978150893350145	0.0	0.0	49.466759324401266	0.0
36	0.001978150893350145	0.0	0.0	50.273500862645804	0.0
37	0.001978150893350145	0.0	0.0	51.05220426214112	0.0
38	0.001978150893350145	0.0	0.0	51.83434792405965	0.0
39	0.001978150893350145	0.0	0.0	52.57202619415809	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACCG	20	7.0333196E-4	45.000004	8
GCGTTGT	20	7.0333196E-4	45.000004	40
CTGCGCG	20	7.0333196E-4	45.000004	1
CCCGTTA	20	7.0333196E-4	45.000004	14
TCGTGCG	20	7.0333196E-4	45.000004	1
CTACGGA	20	7.0333196E-4	45.000004	3
ACCGTTG	20	7.0333196E-4	45.000004	18
TCGAGAG	20	7.0333196E-4	45.000004	1
CGTCATA	20	7.0333196E-4	45.000004	38
TCGGTCG	20	7.0333196E-4	45.000004	16
CCGCACG	20	7.0333196E-4	45.000004	1
ATCGACC	20	7.0333196E-4	45.000004	10
AGTCACG	25	3.8908336E-5	45.0	1
CGAAACG	25	3.8908336E-5	45.0	3
ATTAACG	25	3.8908336E-5	45.0	1
ACTACGG	35	1.211938E-7	45.0	2
TATCGAG	35	1.211938E-7	45.0	1
TCGAAAC	25	3.8908336E-5	45.0	2
ACGCGAG	30	2.1655233E-6	44.999996	1
GATCGAC	30	2.1655233E-6	44.999996	9
>>END_MODULE