FastQCFastQC Report
Sat 18 Jun 2016
SRR3552416_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552416_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences836010
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA69400.8301336108419756No Hit
GCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTGC46940.5614765373619932No Hit
CTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTGCT38570.46135811772586455No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTC30830.3687754931161111No Hit
CCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTGC29640.3545412136218466No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29260.3499958134472076No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCG28330.33887154459874885No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGC28290.338393081422471No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23430.28025980550471885No Hit
TCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTGC18300.21889690314709156No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCC18170.21734189782418872No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT15630.1869594861305487No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT13080.1564574586428392No Hit
ACTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTGC12650.15131397949785289No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA11350.13576392626882455No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT9100.10885037260319852No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCGC253.88989E-545.028
TCCGATC207.032183E-445.014
GCAAACG207.032183E-445.01
TCTAGCG253.88989E-545.01
ACGATTG406.8121153E-945.01
CGTAATT207.032183E-445.024
CGATGCA207.032183E-445.010
ATCGGCA207.032183E-445.022
GCCGTAA207.032183E-445.022
AATCGGC207.032183E-445.021
TAGTGCG603.6379788E-1241.2500041
CGTTTTT15200.040.2631571
AATACGG451.9272193E-840.02
TCACCGC403.4567165E-739.37524
TGCGAAG403.4567165E-739.3751
GTATCCG403.4567165E-739.3751
ACCCGAA403.4567165E-739.37531
TACGAAA1900.039.07894520
TCGATTG356.2462477E-638.5714261
AGGAACG356.2462477E-638.5714261
Produced by FastQC (version 0.11.5)