FastQCFastQC Report
Sat 18 Jun 2016
SRR3552415_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552415_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1013188
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTGC46700.46092136898581504TruSeq Adapter, Index 23 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTGCT40800.4026893330754016TruSeq Adapter, Index 20 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39030.3852197223022775No Hit
CCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTGC29080.28701484818217354TruSeq Adapter, Index 23 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27420.27063091943449785No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCC24870.24546283611728525No Hit
TCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTGC22500.22207132338716998TruSeq Adapter, Index 23 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTGC13320.13146622344520464TruSeq Adapter, Index 20 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTC12800.12633390841581227No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12110.11952372116527238No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGC12060.11903022933552312No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCG11310.11162785188928413No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA11130.10985128130218676No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTTG302.1652468E-645.00000410
TATCCGG302.1652468E-645.0000042
TAGCGGT351.211738E-745.03
CACGATT207.03289E-445.036
CTACGCG207.03289E-445.01
TCCGTCG207.03289E-445.045
TATAACG750.042.01
TCTAGCG700.041.7857131
TTACCCG603.6379788E-1241.25000427
CCCTCGT1150.041.08695614
ACGACCA556.184564E-1140.90909228
CGATTCG501.0804797E-940.510
ACGATAG451.9279469E-840.01
CGTTTTA451.9279469E-840.012
AATAGCG403.4576806E-739.3751
CGTGTAC1150.039.13043218
TAGCGCG750.039.01
GGCGATT3950.038.7341778
CGGCGTA700.038.57142641
CAAACGT356.2475556E-638.57142614
Produced by FastQC (version 0.11.5)