##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552415_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1013188
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.163395144829984	31.0	31.0	34.0	28.0	34.0
2	31.344221408070368	31.0	31.0	34.0	28.0	34.0
3	31.389649304966106	31.0	31.0	34.0	28.0	34.0
4	35.15038966114877	37.0	35.0	37.0	32.0	37.0
5	34.981389436116494	35.0	35.0	37.0	32.0	37.0
6	35.094931049321545	36.0	35.0	37.0	32.0	37.0
7	35.548099661662	37.0	35.0	37.0	35.0	37.0
8	35.58174099969601	37.0	35.0	37.0	35.0	37.0
9	37.07716040853228	39.0	37.0	39.0	33.0	39.0
10	36.47344717860851	38.0	35.0	39.0	32.0	39.0
11	36.23109630196962	38.0	35.0	39.0	32.0	39.0
12	36.250662266035526	38.0	35.0	39.0	32.0	39.0
13	36.012484356308995	38.0	35.0	39.0	31.0	39.0
14	37.14423779199912	39.0	36.0	40.0	32.0	41.0
15	37.255778789326364	39.0	36.0	40.0	32.0	41.0
16	37.24055357939494	39.0	36.0	40.0	32.0	41.0
17	37.23662933236477	39.0	36.0	40.0	32.0	41.0
18	37.181422401370725	39.0	36.0	40.0	32.0	41.0
19	37.11395318539106	39.0	36.0	40.0	31.0	41.0
20	36.71596386850219	39.0	35.0	40.0	31.0	41.0
21	36.81971559078868	39.0	35.0	40.0	31.0	41.0
22	36.91768556279782	39.0	35.0	40.0	31.0	41.0
23	36.74403664472931	39.0	35.0	40.0	31.0	41.0
24	36.77495686881408	39.0	35.0	40.0	31.0	41.0
25	36.794353071690544	39.0	35.0	40.0	31.0	41.0
26	36.73679613260323	39.0	35.0	40.0	31.0	41.0
27	36.53601602071876	38.0	35.0	40.0	30.0	41.0
28	36.50557744465983	38.0	35.0	40.0	30.0	41.0
29	36.53080672096393	38.0	35.0	40.0	30.0	41.0
30	36.36324847905818	38.0	35.0	40.0	30.0	41.0
31	36.268017386704145	38.0	35.0	40.0	30.0	41.0
32	35.9801902509702	38.0	35.0	40.0	29.0	41.0
33	35.87502516808332	38.0	35.0	40.0	29.0	41.0
34	35.82019625183086	38.0	35.0	40.0	29.0	41.0
35	35.62321109211716	38.0	34.0	40.0	27.0	41.0
36	35.688092436941616	38.0	34.0	40.0	28.0	41.0
37	35.7435421659159	38.0	34.0	40.0	29.0	41.0
38	35.69155971053743	38.0	34.0	40.0	28.0	41.0
39	35.64702009893524	38.0	34.0	40.0	28.0	41.0
40	35.53073368417312	38.0	34.0	40.0	27.0	41.0
41	35.48497909568609	38.0	34.0	40.0	27.0	41.0
42	35.51349897551096	38.0	34.0	40.0	28.0	41.0
43	35.43369937267319	38.0	34.0	40.0	27.0	41.0
44	35.322978558766984	38.0	34.0	40.0	27.0	41.0
45	35.21132899323719	38.0	34.0	40.0	26.0	41.0
46	35.057779010410705	38.0	34.0	40.0	26.0	41.0
47	34.959725144790504	38.0	33.0	40.0	26.0	41.0
48	34.89329917053893	37.0	33.0	40.0	26.0	41.0
49	34.852550563172876	37.0	33.0	40.0	26.0	41.0
50	34.74586256449938	37.0	33.0	40.0	26.0	41.0
51	33.45715306537385	36.0	31.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	10.0
9	45.0
10	73.0
11	50.0
12	34.0
13	20.0
14	32.0
15	52.0
16	123.0
17	248.0
18	498.0
19	855.0
20	1462.0
21	2202.0
22	3261.0
23	4774.0
24	6640.0
25	8932.0
26	11129.0
27	13586.0
28	16904.0
29	21097.0
30	26435.0
31	33862.0
32	42712.0
33	55350.0
34	77251.0
35	90601.0
36	101350.0
37	133233.0
38	187246.0
39	173101.0
40	20.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.343387406878094	19.386135643138292	25.331922604689357	11.938554345294259
2	33.05299707458043	23.537388914989123	25.391141624259266	18.018472386171176
3	31.64703885162477	24.303979123321636	27.642747446673273	16.40623457838032
4	27.843302526283374	25.717241025357584	27.800862228924938	18.638594219434104
5	23.775745468758018	29.85971014263888	26.470506954286865	19.894037434316235
6	24.592474446993055	36.15666589023952	25.233915127301152	14.016944535466273
7	77.10069602087668	7.022586134063964	10.65981831604796	5.216899529011398
8	77.51384738074276	6.501754856946588	10.13503910429259	5.849358658018057
9	72.39386964709412	8.05526713699728	12.682542627824253	6.868320588084344
10	36.0714892004248	25.745468758019243	21.6708054181455	16.512236623410462
11	27.90587729029558	27.128923753538338	24.709135915545783	20.2560630406203
12	23.44046711962637	24.7984579367304	32.127798592166506	19.633276351476724
13	22.886374493183894	24.79204254294366	34.36844889596008	17.953134067912373
14	20.420099724828955	29.11493227318129	30.317571862280246	20.14739613970951
15	19.859493006233787	27.53062610295424	33.246347173476195	19.363533717335777
16	21.901167404272453	26.397470163483973	30.763787174739537	20.937575257504037
17	21.178596667153577	26.883460917421054	28.71619087474388	23.221751540681492
18	21.37263765461099	26.663955751548578	31.428027177582045	20.535379416258383
19	21.443503081362987	28.89779586809161	29.18056668653794	20.47813436400747
20	22.38281543010774	28.355547045563114	29.689060667911583	19.572576856417566
21	21.935613133988955	29.066866168963706	29.865039854400173	19.13248084264717
22	19.860677386625188	26.940804668037917	28.460562106933757	24.73795583840314
23	19.89166867353344	28.685890476397276	29.060944267006718	22.36149658306257
24	21.107237748571837	27.175805477364516	28.933722073297353	22.783234700766293
25	20.011192394698714	28.89848675665326	27.881893587369767	23.20842726127826
26	19.03773041133531	29.014654733376233	29.829212347560375	22.11840250772808
27	21.954958013715125	27.502299671926632	29.2054386747573	21.337303639600943
28	18.45254779961863	27.505260622905126	29.922975795212736	24.11921578226351
29	21.510321875111035	28.759420759029915	27.20857333485987	22.521684030999182
30	22.781458130179196	28.150155746021472	27.576422144754968	21.491963979044364
31	21.538845702870542	29.918830463842838	25.57748413917259	22.964839694114026
32	21.658369424035815	30.86189335049369	25.097020493728706	22.382716731741787
33	21.86366202521151	27.694958882260746	25.560705416961117	24.880673675566626
34	18.960943082626326	27.72427229694785	28.55866828268791	24.756116337737915
35	21.16310102369945	29.25409696917058	26.085978120546237	23.496823886583734
36	21.550492110052627	29.702977137510512	26.101769859098212	22.64476089333865
37	21.564408579651555	27.614618412377567	27.438441829157078	23.382531178813803
38	20.766827084410792	30.549019530432652	25.324915020706918	23.359238364449638
39	21.647413905415384	26.15585656363873	28.218751110356617	23.97797842058927
40	20.542683095338674	27.561025199666794	30.437885170373118	21.458406534621414
41	18.399151983639758	29.219749937820026	27.158829358421144	25.222268720119068
42	20.300872098761534	27.611558763033116	27.177384651219715	24.910184486985635
43	22.433546390205965	24.64517937441028	27.99786416736085	24.923410068022914
44	21.30542406739914	25.11083826496169	27.444955921309766	26.13878174632941
45	22.930887456227275	24.759175987082358	27.454332266075003	24.855604290615364
46	22.246611685096944	27.29375002467459	27.653900362025606	22.80573792820286
47	19.479997789156602	26.905569351393822	30.347970958992804	23.266461900456775
48	19.35494695949814	27.3232608360936	28.528170487609405	24.793621716798857
49	21.12263469366001	24.496144841826	29.98041824419555	24.40080222031844
50	20.077813791714863	22.897922202000025	30.241179327035063	26.783084679250052
51	19.60968744201471	23.400889074880478	27.909726526567624	29.079696956537187
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4504.0
1	4102.5
2	3701.0
3	5206.0
4	6711.0
5	4917.0
6	3123.0
7	2872.0
8	2621.0
9	2626.0
10	2631.0
11	2861.0
12	3091.0
13	3011.0
14	2931.0
15	3129.0
16	3327.0
17	3484.5
18	3642.0
19	3757.5
20	3873.0
21	4001.5
22	4130.0
23	4625.0
24	5120.0
25	6306.0
26	8212.0
27	8932.0
28	10469.0
29	12006.0
30	14309.5
31	16613.0
32	17938.0
33	19263.0
34	22043.5
35	24824.0
36	27033.5
37	29243.0
38	32633.5
39	36024.0
40	39602.5
41	43181.0
42	48760.0
43	54339.0
44	61693.0
45	69047.0
46	83883.0
47	98719.0
48	110905.5
49	123092.0
50	120850.5
51	118609.0
52	100985.0
53	83361.0
54	70715.0
55	58069.0
56	50014.5
57	41960.0
58	37396.5
59	32833.0
60	29277.5
61	25722.0
62	22438.5
63	19155.0
64	16073.0
65	12991.0
66	11269.5
67	9548.0
68	7871.5
69	6195.0
70	5338.5
71	4482.0
72	3885.0
73	3288.0
74	2595.0
75	1636.5
76	1371.0
77	1011.5
78	652.0
79	555.5
80	459.0
81	337.5
82	216.0
83	162.5
84	109.0
85	73.5
86	38.0
87	32.5
88	27.0
89	20.0
90	13.0
91	9.0
92	5.0
93	4.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1013188.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.678632376575784
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.76698583030134	22.798896819701888
2	12.568869992058218	8.21466963718891
3	5.208980687535561	5.106670948339728
4	2.8160188477884533	3.680945787695496
5	1.5892384055183395	2.596706880633464
6	1.0625657253597882	2.0833916828989265
7	0.7322283229719679	1.674975412748228
8	0.5427731655449257	1.418966779257035
9	0.45421934343267023	1.3358940248129267
>10	4.827077724131885	35.09710141639528
>50	0.35475972470876604	7.383915500839526
>100	0.06586618308991397	4.156667201833367
>500	0.006127086798934578	1.3152768231472791
>1k	0.004288960759254204	3.1359210845079444
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTGC	4670	0.46092136898581504	TruSeq Adapter, Index 23 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTGCT	4080	0.4026893330754016	TruSeq Adapter, Index 20 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3903	0.3852197223022775	No Hit
CCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTGC	2908	0.28701484818217354	TruSeq Adapter, Index 23 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2742	0.27063091943449785	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCC	2487	0.24546283611728525	No Hit
TCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTGC	2250	0.22207132338716998	TruSeq Adapter, Index 23 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTGC	1332	0.13146622344520464	TruSeq Adapter, Index 20 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTC	1280	0.12633390841581227	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1211	0.11952372116527238	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGC	1206	0.11903022933552312	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCG	1131	0.11162785188928413	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1113	0.10985128130218676	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.869836594985333E-5	0.0	0.0	0.4559864506883224	0.0
2	9.869836594985333E-5	0.0	0.0	1.6841889165682973	0.0
3	9.869836594985333E-5	0.0	0.0	2.249730553460957	0.0
4	9.869836594985333E-5	0.0	0.0	2.9256169634855524	0.0
5	9.869836594985333E-5	0.0	0.0	4.7203480499176855	0.0
6	9.869836594985333E-5	0.0	0.0	5.765070253496883	0.0
7	9.869836594985333E-5	0.0	0.0	6.858944243319107	0.0
8	9.869836594985333E-5	0.0	0.0	8.531289355973422	0.0
9	9.869836594985333E-5	0.0	0.0	9.100482832406227	0.0
10	9.869836594985333E-5	0.0	0.0	10.883172718192478	0.0
11	9.869836594985333E-5	0.0	0.0	13.436894238778983	0.0
12	9.869836594985333E-5	0.0	0.0	15.307129575162753	0.0
13	9.869836594985333E-5	0.0	0.0	16.013908573729655	0.0
14	9.869836594985333E-5	0.0	0.0	16.271017817029023	0.0
15	9.869836594985333E-5	0.0	0.0	16.828268791181895	0.0
16	9.869836594985333E-5	0.0	0.0	17.99004725677762	0.0
17	9.869836594985333E-5	0.0	0.0	19.427194163373432	0.0
18	9.869836594985333E-5	0.0	0.0	21.061244310039203	0.0
19	9.869836594985333E-5	0.0	0.0	21.99858269146496	0.0
20	9.869836594985333E-5	0.0	0.0	22.8800577977631	0.0
21	4.934918297492667E-4	0.0	0.0	23.95221814707636	0.0
22	4.934918297492667E-4	0.0	0.0	25.075208154853787	0.0
23	4.934918297492667E-4	0.0	0.0	26.222379262288932	0.0
24	9.869836594985333E-4	0.0	0.0	27.12004090060285	0.0
25	0.0013817771232979468	0.0	0.0	27.93410502295724	0.0
26	0.0013817771232979468	0.0	0.0	28.650951254851023	0.0
27	0.0013817771232979468	0.0	0.0	29.36177688642187	0.0
28	0.0014804754892478	0.0	0.0	30.125208747043985	0.0
29	0.0014804754892478	0.0	0.0	30.866532173693333	0.0
30	0.0014804754892478	0.0	0.0	31.646249264697175	0.0
31	0.0014804754892478	0.0	0.0	32.42754552955621	0.0
32	0.0014804754892478	0.0	0.0	33.21505979147009	0.0
33	0.0014804754892478	0.0	0.0	34.00800246351121	0.0
34	0.0015791738551976533	0.0	0.0	34.73817297480823	0.0
35	0.0015791738551976533	0.0	0.0	35.52815469587085	0.0
36	0.0015791738551976533	0.0	0.0	36.31251061007434	0.0
37	0.00177657058709736	0.0	0.0	37.037252711244115	0.0
38	0.00177657058709736	0.0	0.0	37.77146985554507	0.0
39	0.00177657058709736	0.0	0.0	38.488118690706955	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGTTG	30	2.1652468E-6	45.000004	10
TATCCGG	30	2.1652468E-6	45.000004	2
TAGCGGT	35	1.211738E-7	45.0	3
CACGATT	20	7.03289E-4	45.0	36
CTACGCG	20	7.03289E-4	45.0	1
TCCGTCG	20	7.03289E-4	45.0	45
TATAACG	75	0.0	42.0	1
TCTAGCG	70	0.0	41.785713	1
TTACCCG	60	3.6379788E-12	41.250004	27
CCCTCGT	115	0.0	41.086956	14
ACGACCA	55	6.184564E-11	40.909092	28
CGATTCG	50	1.0804797E-9	40.5	10
ACGATAG	45	1.9279469E-8	40.0	1
CGTTTTA	45	1.9279469E-8	40.0	12
AATAGCG	40	3.4576806E-7	39.375	1
CGTGTAC	115	0.0	39.130432	18
TAGCGCG	75	0.0	39.0	1
GGCGATT	395	0.0	38.734177	8
CGGCGTA	70	0.0	38.571426	41
CAAACGT	35	6.2475556E-6	38.571426	14
>>END_MODULE