FastQCFastQC Report
Sat 18 Jun 2016
SRR3552412_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552412_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences790240
Sequences flagged as poor quality0
Sequence length51
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9689312.261211783761896No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT564567.144158736586354No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTC208852.642867989471553No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA142361.8014780319902814No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCG122041.544340959708443No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGC121131.5328254707430653No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCT85041.0761287710062766No Hit
GAACTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTCT60760.7688803401498279No Hit
CGCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTCTG55980.7083923871228994No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTC50240.6357562259566715No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCC47770.6044998987649322No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTT45540.5762806236080178No Hit
CGTCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTCT44460.562613889451306No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39050.49415367483296213No Hit
GCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTCTGC37960.4803603968414659No Hit
CTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTCTGCT33560.42468110953634336No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCT33180.4198724438145373No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31710.4012705001012351No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTT30150.38152966187487347No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGT28320.3583721401093339No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCG27600.3492609840048593No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTC27530.3483751771613687No Hit
CCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTCTGC27180.3439461429439158No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTC25750.32585037456975097No Hit
AAAACTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTC23860.30193358979550516No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCATTCGTCGTA21180.2680198420732942No Hit
GAAAACTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTT18490.23397955051629882No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18460.23359991901194577No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGC17650.22334986839441184No Hit
AAAAACTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTT12750.16134338935007086No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCATTCGT12470.15780016197610852No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12030.15223223324559626No Hit
CATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10340.13084632516703787No Hit
GAAACTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTC8940.11313018829722617No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8150.10313322534926098No Hit
ACTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTCTGC8110.10262705001012351No Hit
CTGAATGATACCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGC8080.10224741850577039No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATGC551.8189894E-1245.0000048
CGTGTAC551.8189894E-1245.00000418
GCCCGAA351.2112832E-745.00000425
GTTAGCG207.031948E-445.01
CCGTCGA207.031948E-445.041
CTACGAA253.889696E-545.011
TACGAAT253.889696E-545.012
CGCTTGG207.031948E-445.02
TGCGTAG253.889696E-545.01
CCTCGAT302.1646374E-644.99999615
CTCGATC302.1646374E-644.99999616
CGTTTAC302.1646374E-644.99999633
CGTTTTT118750.044.4694751
TACGGGA800.042.18754
CGTCTGT5550.041.7567561
CGTTTTC8250.041.4545441
CGAAAGG556.002665E-1140.9090922
GTGGCGT556.002665E-1140.90909234
CACAACG501.0804797E-940.512
CGTTTCT4700.040.2127651
Produced by FastQC (version 0.11.5)