##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552412_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	790240
Sequences flagged as poor quality	0
Sequence length	51
%GC	33
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.203911469933185	31.0	31.0	34.0	28.0	34.0
2	31.44845110346224	31.0	31.0	34.0	30.0	34.0
3	31.108496153067424	31.0	31.0	34.0	28.0	34.0
4	35.17251088276979	37.0	35.0	37.0	32.0	37.0
5	35.05691562057097	35.0	35.0	37.0	32.0	37.0
6	35.14991901194574	37.0	35.0	37.0	32.0	37.0
7	35.05700167037862	37.0	35.0	37.0	32.0	37.0
8	34.719552287912535	36.0	35.0	37.0	31.0	37.0
9	36.106101943713306	38.0	35.0	39.0	31.0	39.0
10	36.58200926300871	38.0	35.0	39.0	32.0	39.0
11	36.448621937639196	38.0	35.0	39.0	32.0	39.0
12	36.47412558210164	38.0	35.0	39.0	32.0	39.0
13	36.215639552541	38.0	35.0	39.0	31.0	39.0
14	37.48313929945333	39.0	36.0	41.0	32.0	41.0
15	37.413480714719576	39.0	36.0	41.0	32.0	41.0
16	37.40129580886819	39.0	36.0	41.0	32.0	41.0
17	37.11338454140514	39.0	36.0	40.0	31.0	41.0
18	36.57583392387123	38.0	36.0	40.0	31.0	41.0
19	35.99177465073902	37.0	35.0	40.0	30.0	41.0
20	35.34368293176757	35.0	34.0	40.0	29.0	41.0
21	35.46715807855841	35.0	34.0	40.0	30.0	41.0
22	35.49578862117838	35.0	34.0	40.0	30.0	41.0
23	35.1647271714922	35.0	34.0	40.0	29.0	41.0
24	35.07407369912938	35.0	33.0	40.0	28.0	41.0
25	35.12010908078558	35.0	33.0	40.0	29.0	41.0
26	35.019873709252884	35.0	33.0	40.0	28.0	41.0
27	34.7976829823851	35.0	33.0	40.0	27.0	41.0
28	34.71745672200851	35.0	33.0	40.0	26.0	41.0
29	34.700031635958695	35.0	33.0	40.0	25.0	41.0
30	34.53562841668354	35.0	33.0	40.0	25.0	41.0
31	34.416174832962135	35.0	33.0	40.0	25.0	41.0
32	33.99369052439765	35.0	33.0	40.0	23.0	41.0
33	33.783206367685764	35.0	33.0	39.0	22.0	41.0
34	33.586826786798945	35.0	33.0	39.0	21.0	41.0
35	33.37245773435918	35.0	33.0	39.0	21.0	41.0
36	33.3265843288115	35.0	33.0	39.0	21.0	41.0
37	33.25016830330026	35.0	33.0	39.0	21.0	41.0
38	33.16073724438145	35.0	33.0	39.0	20.0	41.0
39	33.04854854221502	35.0	33.0	39.0	20.0	41.0
40	32.850984511034625	35.0	33.0	39.0	18.0	41.0
41	32.671980664102044	35.0	32.0	39.0	18.0	41.0
42	32.67208822636161	35.0	32.0	39.0	18.0	41.0
43	32.53730385705608	35.0	32.0	39.0	18.0	40.0
44	32.476200900992104	35.0	32.0	39.0	17.0	40.0
45	32.417966693662684	35.0	31.0	39.0	18.0	40.0
46	32.25833923871229	35.0	31.0	39.0	17.0	40.0
47	31.9712732840656	35.0	31.0	38.0	15.0	40.0
48	31.907173769993925	35.0	31.0	38.0	15.0	40.0
49	31.790010629682122	35.0	31.0	38.0	15.0	40.0
50	31.599557096578256	35.0	31.0	38.0	12.0	40.0
51	30.67345743065398	35.0	28.0	37.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	17.0
9	41.0
10	77.0
11	102.0
12	79.0
13	63.0
14	55.0
15	97.0
16	210.0
17	436.0
18	956.0
19	1761.0
20	2992.0
21	4456.0
22	6527.0
23	8462.0
24	10935.0
25	14145.0
26	17566.0
27	19432.0
28	20648.0
29	23117.0
30	26982.0
31	33167.0
32	41785.0
33	53217.0
34	72840.0
35	127216.0
36	65554.0
37	66812.0
38	88664.0
39	81826.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.26609637578457	27.802945940473776	12.032420530471756	26.898537153269892
2	34.087239319700345	44.43612067220085	12.598324559627455	8.878315448471351
3	16.02867483296214	44.2082152257542	31.091693662684754	8.671416278598906
4	13.85452520753189	35.33154484713505	40.80886819194169	10.005061753391374
5	18.792645272322332	36.38046669366268	31.142690828102854	13.684197205912128
6	13.55385705608423	44.091541810083015	32.47949989876493	9.875101235067827
7	40.63107410406965	25.01569143551326	28.785432273739627	5.567802186677465
8	39.113560437335494	29.619229601133835	25.13236485118445	6.134845110346224
9	36.65810386717959	25.393677870014177	24.56380340149828	13.384414861307958
10	23.076154079773232	34.05535027333468	29.824357157319298	13.044138489572788
11	18.116141931565092	34.716288722413445	31.20014172909496	15.967427616926502
12	14.157597691840454	34.05826078153472	35.520727880137684	16.263413646487145
13	13.654839036242155	35.42354221502328	38.622317270702574	12.29930147803199
14	11.982815347236283	40.86505365458595	32.61452217047985	14.537608827697914
15	10.116673415671189	37.97352702976311	38.43490585138692	13.47489370317878
16	11.04259465478842	40.52224640615509	30.999316663292163	17.435842275764323
17	10.92592123911723	39.82600222717149	32.48848451103462	16.759592022676657
18	11.121558007693865	40.488838833772014	31.048036039684145	17.34156711884997
19	11.35566410204495	40.88897043936019	29.56152561247216	18.193839846122696
20	12.683108928932981	40.72041405142742	32.21869305527434	14.377783964365257
21	12.52783964365256	42.699686171289734	30.856195586151042	13.916278598906661
22	11.430831139906864	37.80648916784774	31.4572788013768	19.305400890868597
23	12.837745495039481	41.12358270905042	31.174832962138083	14.86383883377202
24	15.21727576432476	38.19839542417493	28.600425187284877	17.98390362421543
25	13.302414456367687	43.5143753796315	27.647929742862924	15.535280421137882
26	14.044594047378013	37.39661368698117	29.799301478031992	18.75949078760883
27	16.98129682121887	38.46363130188297	27.594907876088275	16.96016400080988
28	12.97352702976311	37.28981069042317	31.406408179793484	18.330254100020245
29	16.309728690018222	35.896309981777684	30.69763616116623	17.09632516703786
30	16.387426604575825	37.93265337112776	30.839871431463862	14.840048592832558
31	15.871127758655598	36.424883579672	28.073623203077545	19.630365458594856
32	16.120419113180805	40.851133832759665	27.779535331038673	15.248911723020855
33	14.495343186879936	36.148511844502934	30.038848957278802	19.31729601133833
34	13.962846730107309	36.63519943308362	33.21775663089694	16.184197205912128
35	15.957683741648108	35.65068839846123	29.281863737598705	19.10976412229196
36	17.552642235270298	35.33635351285685	30.11275055679287	16.998253695079978
37	16.59052945940474	37.59300971856651	30.00949078760883	15.806970034419923
38	15.043404535331039	36.822990483903624	28.706468920834176	19.42713605993116
39	17.192751569143553	37.425718768981575	30.88593338732537	14.495596274549506
40	14.684146588378214	35.41101437537963	33.336075116420325	16.568763919821826
41	16.016273537153268	37.49544442194776	30.04947863940069	16.438803401498276
42	15.601715934399676	35.82810285482891	31.500936424377407	17.069244786394005
43	19.116217857865962	35.093895525410005	28.457810285482893	17.33207633124114
44	16.485877708038064	34.39043834784369	29.955076938651548	19.168607005466693
45	16.32795100222717	33.17055578052237	28.754555578052237	21.74693763919822
46	19.122671593439968	35.24676047782952	28.339238712289937	17.291329216440577
47	13.790240939461428	33.783154484713506	34.96962947965175	17.456975096173313
48	15.356347438752785	34.89382972261591	30.186019437133023	19.563803401498276
49	14.163545252075318	33.00263211176351	34.98063879327799	17.853183842883176
50	14.876493217250456	32.12694877505568	33.27065195383681	19.725906053857056
51	14.141146993318484	32.30967807248431	31.149777282850778	22.399397651346426
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	104411.0
1	71504.5
2	38598.0
3	55781.5
4	72965.0
5	43668.5
6	14372.0
7	11538.5
8	8705.0
9	8271.0
10	7837.0
11	7509.0
12	7181.0
13	6860.5
14	6540.0
15	6078.5
16	5617.0
17	5245.5
18	4874.0
19	4431.5
20	3989.0
21	3707.0
22	3425.0
23	3309.5
24	3194.0
25	3519.0
26	4043.5
27	4243.0
28	4907.0
29	5571.0
30	6134.5
31	6698.0
32	6769.5
33	6841.0
34	8016.0
35	9191.0
36	9986.0
37	10781.0
38	11688.5
39	12596.0
40	14791.5
41	16987.0
42	22157.0
43	27327.0
44	34905.5
45	42484.0
46	79884.0
47	117284.0
48	85376.0
49	53468.0
50	49520.0
51	45572.0
52	36116.0
53	26660.0
54	23950.0
55	21240.0
56	20082.0
57	18924.0
58	17740.5
59	16557.0
60	16159.0
61	15761.0
62	14429.5
63	13098.0
64	11409.0
65	9720.0
66	8926.5
67	8133.0
68	6818.0
69	5503.0
70	4639.5
71	3776.0
72	3206.5
73	2637.0
74	2100.5
75	1277.5
76	991.0
77	806.0
78	621.0
79	426.5
80	232.0
81	173.5
82	115.0
83	94.5
84	74.0
85	47.0
86	20.0
87	14.0
88	8.0
89	6.5
90	5.0
91	3.5
92	2.0
93	1.0
94	0.0
95	1.5
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	790240.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.900119834551518
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.68087745918913	19.043825197968353
2	7.31035925503596	3.494369244579645
3	2.7683340914552064	1.9849054958356118
4	1.5204596233216212	1.453566688039352
5	1.0898624832459074	1.3023921976379544
6	0.8199991766558676	1.175884715178529
7	0.643429843471022	1.0764635254858903
8	0.5244511829443281	1.0027556895793395
9	0.45818025004234614	0.9855506593653166
>10	4.971200580470088	22.788412110512603
>50	0.10882354649218635	1.7849284915577424
>100	0.07308247975210512	3.552517677768908
>500	0.013336218932865896	2.018244144382781
>1k	0.012269321418236626	7.9938022494413135
>5k	0.0021337950292585435	3.21312627458843
>10k+	0.0032006925438878148	27.12925563807826
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	96893	12.261211783761896	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	56456	7.144158736586354	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTC	20885	2.642867989471553	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	14236	1.8014780319902814	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCG	12204	1.544340959708443	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGC	12113	1.5328254707430653	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCT	8504	1.0761287710062766	No Hit
GAACTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTCT	6076	0.7688803401498279	No Hit
CGCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTCTG	5598	0.7083923871228994	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTC	5024	0.6357562259566715	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCC	4777	0.6044998987649322	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTT	4554	0.5762806236080178	No Hit
CGTCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTCT	4446	0.562613889451306	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3905	0.49415367483296213	No Hit
GCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTCTGC	3796	0.4803603968414659	No Hit
CTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTCTGCT	3356	0.42468110953634336	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCT	3318	0.4198724438145373	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3171	0.4012705001012351	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTT	3015	0.38152966187487347	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGT	2832	0.3583721401093339	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCG	2760	0.3492609840048593	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTC	2753	0.3483751771613687	No Hit
CCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTCTGC	2718	0.3439461429439158	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTC	2575	0.32585037456975097	No Hit
AAAACTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTC	2386	0.30193358979550516	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCATTCGTCGTA	2118	0.2680198420732942	No Hit
GAAAACTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTT	1849	0.23397955051629882	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1846	0.23359991901194577	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGC	1765	0.22334986839441184	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTT	1275	0.16134338935007086	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCATTCGT	1247	0.15780016197610852	No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1203	0.15223223324559626	No Hit
CATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1034	0.13084632516703787	No Hit
GAAACTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTC	894	0.11313018829722617	No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	815	0.10313322534926098	No Hit
ACTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTCTGC	811	0.10262705001012351	No Hit
CTGAATGATACCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGC	808	0.10224741850577039	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4869406762502531	0.0
2	0.0	0.0	0.0	1.5867331443612067	0.0
3	0.0	0.0	0.0	2.580102247418506	0.0
4	0.0	0.0	0.0	4.300212593642438	0.0
5	0.0	0.0	0.0	8.48501720996153	0.0
6	0.0	0.0	0.0	10.65081494229601	0.0
7	0.0	0.0	0.0	12.941005264223527	0.0
8	0.0	0.0	0.0	14.640488965377607	0.0
9	0.0	0.0	0.0	15.364319700344199	0.0
10	0.0	0.0	0.0	17.980360396841466	0.0
11	0.0	0.0	0.0	19.24491293784167	0.0
12	0.0	0.0	0.0	21.839314638590807	0.0
13	0.0	0.0	0.0	22.328659647701965	0.0
14	0.0	0.0	0.0	22.63173213201053	0.0
15	0.0	0.0	0.0	23.129302490382667	0.0
16	0.0	0.0	0.0	23.51374266045758	0.0
17	0.0	0.0	0.0	23.847818384288317	0.0
18	0.0	0.0	0.0	24.195560842275764	0.0
19	0.0	0.0	0.0	24.610244988864142	0.0
20	0.0	0.0	0.0	24.84624924073699	0.0
21	0.0	0.0	0.0	25.073775055679288	0.0
22	0.0	0.0	0.0	25.330405952621987	0.0
23	0.0	0.0	0.0	25.5392032800162	0.0
24	0.0	0.0	0.0	25.713580684349058	0.0
25	0.0	0.0	0.0	25.879226564081797	0.0
26	0.0	0.0	0.0	26.031585341162177	0.0
27	0.0	0.0	0.0	26.197737396234057	0.0
28	0.0	0.0	0.0	26.348830734966594	0.0
29	0.0	0.0	0.0	26.510047580481878	0.0
30	0.0	0.0	0.0	26.730360396841466	0.0
31	0.0	0.0	0.0	26.887401295808868	0.0
32	0.0	0.0	0.0	27.044568738611055	0.0
33	0.0	0.0	0.0	27.202115812917594	0.0
34	1.265438347843693E-4	0.0	0.0	27.350172099615307	0.0
35	2.530876695687386E-4	0.0	0.0	27.523030977930755	0.0
36	2.530876695687386E-4	0.0	0.0	27.675642842680706	0.0
37	2.530876695687386E-4	0.0	0.0	27.83977019639603	0.0
38	2.530876695687386E-4	0.0	0.0	28.001746304920026	0.0
39	2.530876695687386E-4	0.0	0.0	28.169416886009312	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATGC	55	1.8189894E-12	45.000004	8
CGTGTAC	55	1.8189894E-12	45.000004	18
GCCCGAA	35	1.2112832E-7	45.000004	25
GTTAGCG	20	7.031948E-4	45.0	1
CCGTCGA	20	7.031948E-4	45.0	41
CTACGAA	25	3.889696E-5	45.0	11
TACGAAT	25	3.889696E-5	45.0	12
CGCTTGG	20	7.031948E-4	45.0	2
TGCGTAG	25	3.889696E-5	45.0	1
CCTCGAT	30	2.1646374E-6	44.999996	15
CTCGATC	30	2.1646374E-6	44.999996	16
CGTTTAC	30	2.1646374E-6	44.999996	33
CGTTTTT	11875	0.0	44.469475	1
TACGGGA	80	0.0	42.1875	4
CGTCTGT	555	0.0	41.756756	1
CGTTTTC	825	0.0	41.454544	1
CGAAAGG	55	6.002665E-11	40.909092	2
GTGGCGT	55	6.002665E-11	40.909092	34
CACAACG	50	1.0804797E-9	40.5	12
CGTTTCT	470	0.0	40.212765	1
>>END_MODULE