SRR3552411_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3552411_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1114591
Sequences flagged as poor quality	0
Sequence length	51
%GC	30

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	142490	12.784061597482843	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	78523	7.045005746502529	No Hit
GAATCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTC	67674	6.0716442174752885	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCG	36668	3.289816623317432	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGC	35369	3.1732716305801856	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	21122	1.8950449088499726	No Hit
GAACTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCT	17795	1.5965497657885268	Illumina PCR Primer Index 5 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTT	11497	1.0314994468823093	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCT	11261	1.0103257607499072	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCT	10320	0.9259001732474065	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTC	9689	0.8692874785459419	No Hit
GCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTGC	7705	0.6912849646193088	Illumina PCR Primer Index 5 (95% over 23bp)
CGTTTCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTT	7486	0.671636501640512	No Hit
CGCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTG	7341	0.6586272453303499	Illumina PCR Primer Index 5 (95% over 22bp)
CGTCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCT	6793	0.6094612283788403	Illumina PCR Primer Index 5 (95% over 21bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTC	6727	0.6035397737824906	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCC	6092	0.5465682030448837	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCACTGAACTCGTA	6043	0.5421719716021393	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGT	4197	0.37655068092241906	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCG	4044	0.36282367253997205	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCACTGAACT	3935	0.3530443005550915	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTC	3877	0.3478405980310266	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3854	0.34577706082320775	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3561	0.31948939117577657	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCACTGAACTCGT	2940	0.2637738865646681	No Hit
AAAACTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTC	2877	0.2581215889954252	No Hit
CTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTGCT	2829	0.25381507656171637	Illumina PCR Primer Index 5 (95% over 24bp)
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2755	0.24717586989308188	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGC	2495	0.2238489275438255	No Hit
GAAAACTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTT	2328	0.20886585303488006	No Hit
CCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTGC	2196	0.19702294384218066	Illumina PCR Primer Index 5 (95% over 23bp)
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCCACTGAACTCG	2150	0.192895869426543	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCACTGAACTCGTAT	2071	0.1858080677127305	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTT	1720	0.15431669554123442	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC	1718	0.1541372575231632	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATG	1691	0.15171484427920198	No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1688	0.15144568725209517	No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1314	0.11789077787278024	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGTACG	25	3.890729E-5	45.000004	1
GCGCTCG	25	3.890729E-5	45.000004	1
CGACGTC	20	7.0331944E-4	45.0	45
CGCATGG	40	6.8139343E-9	45.0	2
GTTAGCG	40	6.8139343E-9	45.0	1
TGACCGG	20	7.0331944E-4	45.0	2
CATACGT	20	7.0331944E-4	45.0	14
TCGAGAG	20	7.0331944E-4	45.0	1
CATCACG	20	7.0331944E-4	45.0	4
CGTTTTT	17660	0.0	44.43941	1
CGTTTCT	830	0.0	43.64458	1
ACGGCTG	4445	0.0	43.53206	8
CGTTTTC	1350	0.0	43.5	1
CGGCTGT	4525	0.0	43.309395	9
GAATCTG	7880	0.0	43.286804	1
TACGGCT	4500	0.0	43.2	7
AATCTGT	7935	0.0	42.958412	2
TGATACC	4045	0.0	42.941906	4
CGTTCTG	505	0.0	42.772278	1
ATGACTG	1205	0.0	42.759335	3

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content