##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552411_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1114591
Sequences flagged as poor quality	0
Sequence length	51
%GC	30
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.04808849165299	31.0	31.0	34.0	28.0	34.0
2	31.250583397856253	31.0	31.0	34.0	28.0	34.0
3	30.91817985251989	31.0	30.0	34.0	27.0	34.0
4	35.037568040653476	35.0	35.0	37.0	32.0	37.0
5	34.85319816865559	35.0	35.0	37.0	32.0	37.0
6	34.89417912041278	35.0	35.0	37.0	32.0	37.0
7	34.77900862289396	35.0	35.0	37.0	32.0	37.0
8	34.203933999108195	35.0	35.0	37.0	30.0	37.0
9	35.69835751410159	37.0	35.0	39.0	30.0	39.0
10	36.35224580137467	37.0	35.0	39.0	32.0	39.0
11	36.17990545410828	38.0	35.0	39.0	31.0	39.0
12	36.27372641623699	38.0	35.0	39.0	31.0	39.0
13	35.993192121594376	38.0	35.0	39.0	30.0	39.0
14	37.28585822063878	39.0	36.0	40.0	31.0	41.0
15	37.05722906429354	39.0	36.0	40.0	31.0	41.0
16	37.14694538175887	39.0	36.0	40.0	31.0	41.0
17	36.712844442490564	39.0	36.0	40.0	30.0	41.0
18	36.073821697824584	37.0	35.0	39.0	30.0	41.0
19	35.48248550365111	37.0	34.0	39.0	29.0	41.0
20	34.74960142330236	35.0	33.0	39.0	27.0	41.0
21	35.008080093953744	35.0	33.0	39.0	29.0	41.0
22	35.03774927305173	35.0	33.0	39.0	29.0	41.0
23	34.71946121940694	35.0	33.0	39.0	27.0	41.0
24	34.54240075507518	35.0	33.0	39.0	27.0	41.0
25	34.69152720594371	35.0	33.0	39.0	27.0	41.0
26	34.482163412408674	35.0	33.0	39.0	26.0	41.0
27	34.31666503677133	35.0	33.0	39.0	25.0	41.0
28	34.179566316254125	35.0	33.0	39.0	25.0	41.0
29	34.15448805884849	35.0	33.0	39.0	24.0	41.0
30	34.04313241359387	35.0	33.0	39.0	24.0	41.0
31	33.995802047567224	35.0	33.0	39.0	24.0	41.0
32	33.42565927770815	35.0	33.0	39.0	21.0	40.0
33	33.03588042609352	35.0	33.0	39.0	21.0	40.0
34	32.5619819287972	35.0	31.0	38.0	18.0	40.0
35	32.37986848987656	35.0	31.0	38.0	18.0	40.0
36	32.523076177719005	35.0	32.0	39.0	18.0	40.0
37	32.459552427751525	35.0	32.0	39.0	18.0	40.0
38	32.44889919261864	35.0	32.0	39.0	17.0	40.0
39	32.09172333169746	35.0	31.0	38.0	15.0	40.0
40	31.70300316438945	35.0	30.0	38.0	15.0	40.0
41	31.406302401508714	35.0	30.0	38.0	15.0	40.0
42	31.68731220689921	35.0	30.0	38.0	15.0	40.0
43	31.70333512472288	35.0	31.0	38.0	13.0	40.0
44	31.715013848129043	35.0	31.0	38.0	12.0	40.0
45	31.67901858170396	35.0	31.0	39.0	12.0	40.0
46	31.422077694867443	35.0	30.0	38.0	10.0	40.0
47	30.927026146810803	35.0	29.0	38.0	10.0	40.0
48	30.870627880540933	35.0	28.0	38.0	10.0	40.0
49	30.88636459472578	35.0	28.0	38.0	10.0	40.0
50	30.612877728242918	35.0	27.0	38.0	8.0	40.0
51	29.753820011107212	34.0	26.0	37.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	26.0
10	50.0
11	65.0
12	63.0
13	59.0
14	80.0
15	138.0
16	343.0
17	712.0
18	1702.0
19	3102.0
20	5450.0
21	8572.0
22	12034.0
23	15833.0
24	20089.0
25	25242.0
26	29566.0
27	31717.0
28	33348.0
29	36752.0
30	44126.0
31	53339.0
32	66662.0
33	81059.0
34	102264.0
35	171340.0
36	85525.0
37	84840.0
38	108552.0
39	91921.0
40	12.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.85713952472252	26.916869057797882	7.835250778088105	24.39074063939149
2	29.536574402628407	56.31330236831268	7.842069422774811	6.308053806284099
3	9.751828249106623	56.61368161056387	27.68468433712456	5.949805803204942
4	8.91214804354243	32.83993859631021	50.18181557181065	8.066097788336709
5	23.64095888088097	33.41297390702061	29.32097962391586	13.625087588182572
6	10.108909905068316	48.839619196638054	33.22537145912716	7.826099439166474
7	31.419058650213394	25.809018734226274	37.735097448301666	5.036825167258662
8	26.942080099336884	37.232940154729405	28.814067222864708	7.010912523069
9	25.11764405059793	26.421171532876187	25.806237444946174	22.654946971579708
10	20.540180209601548	34.03409860657407	32.1997934668412	13.225927716983179
11	16.344111876015507	33.03705125916143	34.294373451786356	16.32446341303671
12	10.959625548743889	32.36021105499686	38.11469857553129	18.56546482072796
13	11.615740661821242	35.136565789603544	44.341197802602025	8.906495745973187
14	10.174673938691413	45.165805214648245	31.501061824471936	13.158459022188406
15	6.344120847916411	38.55046380241721	43.405697695387815	11.699717654278565
16	7.0719214492132085	43.49936434082098	28.91248897577676	20.516225234189044
17	7.44721606400913	42.53838403504066	34.03481635864636	15.979583542303857
18	6.99521169648777	43.63277650725692	31.1751126646456	18.19689913160971
19	6.640462734761002	43.7914894342409	29.050566530682552	20.51748130031554
20	8.703192471498514	44.07311740360365	35.51203984241753	11.7116502824803
21	8.284832732365505	47.36957323358972	32.32836080678922	12.017233227255558
22	7.48068125437941	37.255728783024445	34.318148989180784	20.945440973415362
23	10.256407955922846	44.110619949380535	33.926704952758456	11.706267141938163
24	14.764519002934708	39.32115009003302	28.00596810848105	17.908362798551217
25	9.353565567997588	51.68146880784072	25.673901906618664	13.291063717543029
26	10.831596522850086	38.174630873567075	30.024735530791112	20.969037072791725
27	16.91678831068975	41.13544789075096	24.205201728705866	17.742562069853427
28	8.878862291190222	38.79988264753618	30.920938712047736	21.40031634922586
29	13.05564103783361	36.478134131712885	25.945301908951357	24.520922921502148
30	9.744650728383775	47.67300292214812	27.168530878142743	15.413815471325357
31	11.96232519372577	37.919290573851754	24.7972574693318	25.32112676309068
32	14.492042372493588	42.111500990049265	29.791914702343732	13.604541935113417
33	16.463079281996716	38.75152410166599	24.32282334955154	20.462573266785753
34	13.788645341654474	44.61466134214254	24.24449865466346	17.352194661539524
35	10.822893778973633	48.546327756100666	23.69111180693187	16.93966665799383
36	10.101552946327397	40.566001340401996	22.972641982574775	26.359803730695834
37	8.791924571434723	42.82512598791844	33.44141483288489	14.94153460776195
38	14.494464785737549	37.3765802881954	24.034915049556293	24.094039876510756
39	16.161443973619022	39.79692999494882	29.692506040332283	14.349119991099874
40	14.9527494838914	41.126027394802215	30.60503808123339	13.316185040072995
41	11.015341053354998	45.54836706917605	25.555741971718778	17.880549905750183
42	12.081292599706977	40.18936094047054	34.956230581441986	12.7731158783805
43	17.21115637933556	35.44358423852337	25.809198172244347	21.536061209896726
44	15.430144330969837	35.047743970658296	28.70057267643468	20.821539021937195
45	13.375399586036494	33.290328021668934	28.0554930014687	25.27877939082587
46	21.69019846741989	36.3440939322137	26.225584093178572	15.740123507187839
47	11.364976031566735	33.157902764332384	39.16145025394965	16.31567095015122
48	14.306593180816998	35.70430767878083	28.4455912527555	21.54350788764668
49	11.475779007725704	31.471454551490186	39.314241726337286	17.738524714446825
50	13.637648249447556	30.43726353433681	34.93478773828247	20.99030047793316
51	12.977047185918423	30.607101618441202	30.467498840381808	25.948352355258564
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	151852.0
1	103252.5
2	54653.0
3	78377.0
4	102101.0
5	62740.0
6	23379.0
7	19748.5
8	16118.0
9	15464.0
10	14810.0
11	14446.0
12	14082.0
13	13453.5
14	12825.0
15	11870.0
16	10915.0
17	9840.5
18	8766.0
19	8142.0
20	7518.0
21	6857.5
22	6197.0
23	5922.0
24	5647.0
25	5654.0
26	6097.0
27	6533.0
28	6798.0
29	7063.0
30	7619.0
31	8175.0
32	9033.0
33	9891.0
34	11212.5
35	12534.0
36	14115.0
37	15696.0
38	16580.0
39	17464.0
40	19583.5
41	21703.0
42	27678.5
43	33654.0
44	44049.5
45	54445.0
46	160229.5
47	266014.0
48	169503.5
49	72993.0
50	64573.0
51	56153.0
52	41787.5
53	27422.0
54	23609.5
55	19797.0
56	17750.0
57	15703.0
58	13743.0
59	11783.0
60	10025.5
61	8268.0
62	7031.5
63	5795.0
64	4721.5
65	3648.0
66	2958.5
67	2269.0
68	1727.5
69	1186.0
70	965.0
71	744.0
72	606.0
73	468.0
74	393.5
75	245.0
76	171.0
77	121.0
78	71.0
79	62.0
80	53.0
81	39.0
82	25.0
83	18.5
84	12.0
85	9.5
86	7.0
87	5.0
88	3.0
89	3.5
90	4.0
91	2.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1114591.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.015007998632477
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.81036738644079	18.13821235774099
2	8.188664216808798	3.7692434489594002
3	3.573602908327907	2.467394985573092
4	2.048706184538312	1.8860395689598832
5	1.4270956262620922	1.642230862661774
6	1.0414809583758688	1.4381815552466422
7	0.8024316091369674	1.2927578931849986
8	0.6140670140919142	1.1306205792817414
9	0.5571211073842642	1.1539932068390242
>10	2.7320923676899285	10.097587244522344
>50	0.09902900748833046	1.5899592038310686
>100	0.07614581047481311	3.6362030997553556
>500	0.013414287855666559	2.094097037996205
>1k	0.008679833318372479	5.116018355510457
>5k	0.0031563030248627196	5.255310041239629
>10k+	0.0039453787810784	39.29215055869741
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	142490	12.784061597482843	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	78523	7.045005746502529	No Hit
GAATCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTC	67674	6.0716442174752885	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCG	36668	3.289816623317432	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGC	35369	3.1732716305801856	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	21122	1.8950449088499726	No Hit
GAACTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCT	17795	1.5965497657885268	Illumina PCR Primer Index 5 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTT	11497	1.0314994468823093	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCT	11261	1.0103257607499072	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCT	10320	0.9259001732474065	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTC	9689	0.8692874785459419	No Hit
GCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTGC	7705	0.6912849646193088	Illumina PCR Primer Index 5 (95% over 23bp)
CGTTTCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTT	7486	0.671636501640512	No Hit
CGCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTG	7341	0.6586272453303499	Illumina PCR Primer Index 5 (95% over 22bp)
CGTCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCT	6793	0.6094612283788403	Illumina PCR Primer Index 5 (95% over 21bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTC	6727	0.6035397737824906	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCC	6092	0.5465682030448837	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCACTGAACTCGTA	6043	0.5421719716021393	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGT	4197	0.37655068092241906	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCG	4044	0.36282367253997205	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCACTGAACT	3935	0.3530443005550915	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTC	3877	0.3478405980310266	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3854	0.34577706082320775	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3561	0.31948939117577657	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCACTGAACTCGT	2940	0.2637738865646681	No Hit
AAAACTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTC	2877	0.2581215889954252	No Hit
CTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTGCT	2829	0.25381507656171637	Illumina PCR Primer Index 5 (95% over 24bp)
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2755	0.24717586989308188	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGC	2495	0.2238489275438255	No Hit
GAAAACTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTT	2328	0.20886585303488006	No Hit
CCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTGC	2196	0.19702294384218066	Illumina PCR Primer Index 5 (95% over 23bp)
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCCACTGAACTCG	2150	0.192895869426543	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCACTGAACTCGTAT	2071	0.1858080677127305	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTT	1720	0.15431669554123442	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC	1718	0.1541372575231632	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATG	1691	0.15171484427920198	No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1688	0.15144568725209517	No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1314	0.11789077787278024	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.29634188684459145	0.0
2	0.0	0.0	0.0	1.3995268219463461	0.0
3	0.0	0.0	0.0	2.3130457719468396	0.0
4	0.0	0.0	0.0	4.955450026063372	0.0
5	0.0	0.0	0.0	12.780831713157562	0.0
6	0.0	0.0	0.0	15.520581092077721	0.0
7	0.0	0.0	0.0	18.12413701528184	0.0
8	0.0	0.0	0.0	20.165513627868876	0.0
9	0.0	0.0	0.0	20.88183019600912	0.0
10	0.0	0.0	0.0	25.270614961003634	0.0
11	0.0	0.0	0.0	26.266765118325914	0.0
12	0.0	0.0	0.0	30.596245618347897	0.0
13	0.0	0.0	0.0	31.12451114354952	0.0
14	0.0	0.0	0.0	31.535334485923535	0.0
15	0.0	0.0	0.0	32.347560674722835	0.0
16	0.0	0.0	0.0	32.84451426577103	0.0
17	0.0	0.0	0.0	33.242328351834885	0.0
18	0.0	0.0	0.0	33.467343626496174	0.0
19	0.0	0.0	0.0	34.152258541473955	0.0
20	0.0	0.0	0.0	34.381400890550886	0.0
21	0.0	0.0	0.0	34.51463361896875	0.0
22	8.97190090356014E-5	0.0	0.0	34.73049755470841	0.0
23	1.794380180712028E-4	0.0	0.0	34.86992089474973	0.0
24	1.794380180712028E-4	0.0	0.0	35.00548631740253	0.0
25	1.794380180712028E-4	0.0	0.0	35.11566126049824	0.0
26	1.794380180712028E-4	0.0	0.0	35.247637922789615	0.0
27	1.794380180712028E-4	0.0	0.0	35.42510212266203	0.0
28	1.794380180712028E-4	0.0	0.0	35.548734917113094	0.0
29	1.794380180712028E-4	0.0	0.0	35.66680513300395	0.0
30	1.794380180712028E-4	0.0	0.0	35.818519977283145	0.0
31	1.794380180712028E-4	0.0	0.0	35.94726675524924	0.0
32	1.794380180712028E-4	0.0	0.0	36.076641566278575	0.0
33	2.691570271068042E-4	0.0	0.0	36.18977723667246	0.0
34	2.691570271068042E-4	0.0	0.0	36.31314087409642	0.0
35	2.691570271068042E-4	0.0	0.0	36.44224652809865	0.0
36	2.691570271068042E-4	0.0	0.0	36.56767370273042	0.0
37	3.588760361424056E-4	0.0	0.0	36.68942239799173	0.0
38	3.588760361424056E-4	0.0	0.0	36.810722498207866	0.0
39	3.588760361424056E-4	0.0	0.0	36.92986934220714	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGTACG	25	3.890729E-5	45.000004	1
GCGCTCG	25	3.890729E-5	45.000004	1
CGACGTC	20	7.0331944E-4	45.0	45
CGCATGG	40	6.8139343E-9	45.0	2
GTTAGCG	40	6.8139343E-9	45.0	1
TGACCGG	20	7.0331944E-4	45.0	2
CATACGT	20	7.0331944E-4	45.0	14
TCGAGAG	20	7.0331944E-4	45.0	1
CATCACG	20	7.0331944E-4	45.0	4
CGTTTTT	17660	0.0	44.43941	1
CGTTTCT	830	0.0	43.64458	1
ACGGCTG	4445	0.0	43.53206	8
CGTTTTC	1350	0.0	43.5	1
CGGCTGT	4525	0.0	43.309395	9
GAATCTG	7880	0.0	43.286804	1
TACGGCT	4500	0.0	43.2	7
AATCTGT	7935	0.0	42.958412	2
TGATACC	4045	0.0	42.941906	4
CGTTCTG	505	0.0	42.772278	1
ATGACTG	1205	0.0	42.759335	3
>>END_MODULE