FastQCFastQC Report
Sat 18 Jun 2016
SRR3552409_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552409_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2083356
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37340.17923004997705624No Hit
GCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC36860.17692607504430352No Hit
CTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGCT33830.16238223328130189No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCC23510.11284677222711817No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22940.1101108019944743No Hit
CCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC21390.10267088294079361No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT30900.036.990291
TATCGTG552.752131E-936.818181
CGTAAGG4150.036.3253022
CACGCCG2500.036.026
CGACACT250.002107500636.027
GACCCGT250.002107500636.031
TTGCGAC250.002107500636.01
AATAGCG751.8189894E-1236.01
TATCCGA950.035.52631430
CGGTAGT2600.035.4807712
CGAATAT2300.035.2173914
CATACGA3900.035.1923118
ATTCGCA458.7044464E-735.016
TACGCGG1800.035.02
GTTCACG653.6743586E-1034.6153871
CGAGGGA12650.034.5059284
ATACGAA4050.034.44444319
TACGGGA6150.034.3902444
CGACAGG2750.034.3636362
GCTTACG1250.034.1999971
Produced by FastQC (version 0.11.5)