##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552409_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2083356
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.317121989712753	31.0	31.0	34.0	30.0	34.0
2	31.51233010584845	31.0	31.0	34.0	30.0	34.0
3	31.580184567591903	31.0	31.0	34.0	30.0	34.0
4	35.298635950840854	37.0	35.0	37.0	33.0	37.0
5	35.11420083749489	37.0	35.0	37.0	32.0	37.0
6	35.21550565529847	37.0	35.0	37.0	32.0	37.0
7	35.64191429597246	37.0	35.0	37.0	35.0	37.0
8	35.69149007658797	37.0	35.0	37.0	35.0	37.0
9	37.18371320120037	39.0	37.0	39.0	34.0	39.0
10	36.559818869170705	38.0	35.0	39.0	32.0	39.0
11	36.29241329854331	38.0	35.0	39.0	32.0	39.0
12	36.304494767096934	38.0	35.0	39.0	32.0	39.0
13	36.083136535474495	38.0	35.0	39.0	31.0	39.0
14	37.282916121872596	39.0	36.0	41.0	32.0	41.0
15	37.37620982683708	39.0	36.0	41.0	32.0	41.0
16	37.33167687135564	39.0	36.0	41.0	32.0	41.0
17	37.344997686425174	39.0	36.0	41.0	32.0	41.0
18	37.29290145323219	39.0	36.0	41.0	32.0	41.0
19	37.24956512473144	39.0	36.0	40.0	32.0	41.0
20	36.86541570427714	39.0	35.0	40.0	31.0	41.0
21	36.96251144787545	39.0	35.0	40.0	31.0	41.0
22	37.04542910573133	39.0	35.0	40.0	31.0	41.0
23	36.88358974654356	39.0	35.0	40.0	31.0	41.0
24	36.93082123266499	39.0	35.0	40.0	31.0	41.0
25	36.94404844875288	39.0	35.0	40.0	31.0	41.0
26	36.89048199155593	39.0	35.0	40.0	31.0	41.0
27	36.679363008530466	39.0	35.0	40.0	30.0	41.0
28	36.641083905007115	39.0	35.0	40.0	30.0	41.0
29	36.69909271387127	39.0	35.0	40.0	30.0	41.0
30	36.55963407118131	39.0	35.0	40.0	30.0	41.0
31	36.468860338799516	38.0	35.0	40.0	30.0	41.0
32	36.244777176824314	38.0	35.0	40.0	30.0	41.0
33	36.17198884876132	38.0	35.0	40.0	30.0	41.0
34	36.147938230432054	38.0	35.0	40.0	30.0	41.0
35	35.98001349745315	38.0	35.0	40.0	29.0	41.0
36	36.04269649546213	38.0	35.0	40.0	29.0	41.0
37	36.0668459927156	38.0	35.0	40.0	30.0	41.0
38	36.03286956237916	38.0	35.0	40.0	29.0	41.0
39	36.00161614241637	38.0	35.0	40.0	29.0	41.0
40	35.891792857293716	38.0	35.0	40.0	29.0	41.0
41	35.813304591246045	38.0	34.0	40.0	29.0	41.0
42	35.78403402970976	38.0	34.0	40.0	29.0	41.0
43	35.6773364705792	38.0	34.0	40.0	28.0	41.0
44	35.60005299142345	38.0	34.0	40.0	28.0	41.0
45	35.551100724024124	38.0	34.0	40.0	28.0	41.0
46	35.43576229890619	38.0	34.0	40.0	28.0	41.0
47	35.34701942442866	38.0	34.0	40.0	27.0	41.0
48	35.28636104439184	38.0	34.0	40.0	27.0	41.0
49	35.212272410476174	38.0	34.0	40.0	27.0	41.0
50	35.11220069925639	37.0	34.0	40.0	27.0	41.0
51	33.869398700942135	36.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	39.0
9	77.0
10	134.0
11	105.0
12	57.0
13	48.0
14	56.0
15	84.0
16	158.0
17	386.0
18	757.0
19	1386.0
20	2345.0
21	3839.0
22	5747.0
23	8215.0
24	11899.0
25	16233.0
26	21181.0
27	26552.0
28	32894.0
29	40899.0
30	51712.0
31	65740.0
32	83073.0
33	107595.0
34	154064.0
35	177489.0
36	201199.0
37	268431.0
38	389952.0
39	410970.0
40	39.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.00064127302295	19.564683136247478	25.202893792515535	11.231781798214035
2	31.490393384519976	23.82074883025273	26.46830402485221	18.22055376037509
3	32.09393881794566	24.386374676243523	26.823500160318254	16.69618634549256
4	28.15058972158383	26.850379867867037	26.18573109924564	18.813299311303496
5	25.048671470454405	30.883247990261864	24.93520070501633	19.132879834267403
6	25.48364273796701	36.524434614151396	23.60340719492972	14.388515452951872
7	82.91261791071713	6.076349889313204	7.029331520873053	3.9817006790966114
8	84.94112384057262	4.914378531561576	6.215212378489323	3.9292852493764867
9	78.88334974915473	7.377567732063076	8.573810716939398	5.165271801842796
10	39.287908547555	28.230940847363577	17.027142744686937	15.454007860394478
11	29.12493112074941	27.419125679912604	24.135433406484537	19.320509792853453
12	25.35908409316507	25.319916519308272	29.258705665282363	20.062293722244302
13	24.155737185579422	25.332924377782767	31.137933219286573	19.373405217351237
14	22.064111942462066	27.606851637454184	29.171154617837757	21.157881802245992
15	21.509525976357377	27.593747780024152	30.97401500271677	19.922711240901698
16	24.60606828597705	26.19859495928684	29.16990663141585	20.02543012332026
17	24.03593048907628	26.581870789245816	27.60958760768683	21.77261111399108
18	24.023834620679327	26.12323577919472	28.968116826888924	20.884812773237027
19	23.48777645299219	28.26660445934348	27.572052016074068	20.67356707159026
20	25.08966302446629	27.628163405582146	27.371414199013515	19.910759370938045
21	24.112201659245947	28.43325864614593	27.7020826013413	19.752457093266827
22	23.22267533729233	26.324929584766117	27.684322794567993	22.76807228337356
23	21.598469008657187	28.359819445164437	27.809793429447488	22.23191811673089
24	21.742659439865296	27.44456540312841	28.706087677766067	22.106687479240225
25	22.284957539661967	28.360347439419858	26.80775633161111	22.546938689307062
26	21.348055733153622	28.73483936494771	27.378806118589427	22.538298783309237
27	22.104143506918643	27.83902511140679	28.22393292360979	21.832898458064776
28	19.975702664355012	27.959263803209822	29.03238812761717	23.032645404817995
29	21.606628919877352	27.033593874498646	28.217548993066956	23.142228212557047
30	22.34394889783599	27.331094637690338	27.905504388112256	22.41945207636141
31	22.074479829659456	27.93094411132807	27.072809447833208	22.92176661117927
32	21.57888522172879	27.802161512482744	27.323654718636657	23.29529854715181
33	21.530213751274385	27.368054235569915	27.047945718350586	24.053786294805114
34	20.200628217164997	26.28269004433232	29.56796630052665	23.948715437976034
35	20.287363273487586	26.43076843323945	28.381323211203462	24.90054508206951
36	21.300824247032192	28.716695562352278	26.99207432623133	22.990405864384194
37	20.46836930414197	28.246444678681897	29.004500431035314	22.280685586140823
38	20.84588519676906	27.501780780625108	28.425050735448	23.227283287157835
39	22.049568100699066	25.574889745199574	28.18658932990809	24.188952824193272
40	21.68822803207901	25.72776808188327	28.976948730797808	23.607055155239912
41	20.36435443582374	26.42529649277416	27.881504649229417	25.328844422172686
42	21.921025499242567	26.81068429975482	27.55923615551063	23.709054045491985
43	21.810338703514905	25.91482204673613	28.89842158517315	23.37641766457581
44	20.809117596800547	26.307361775903875	28.48250611033352	24.401014516962054
45	21.565589366387695	25.057599373318816	28.298476112579895	25.07833514771359
46	21.433974798354193	26.04917258500227	27.941120000614394	24.57573261602914
47	19.940183050808407	26.833820048037875	29.220450081503113	24.005546819650604
48	20.209028125773994	26.35713723434689	29.405536067767585	24.028298572111538
49	21.798242835117954	25.483882735355838	29.061235813754344	23.65663861577186
50	20.124933040728518	24.472389740399624	29.73274850769624	25.669928711175622
51	19.92635920121189	24.30703153949685	28.167101541935224	27.599507717356037
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2657.0
1	3402.0
2	4147.0
3	5790.0
4	7433.0
5	5328.5
6	3224.0
7	3185.5
8	3147.0
9	3200.0
10	3253.0
11	3564.5
12	3876.0
13	4342.5
14	4809.0
15	4785.0
16	4761.0
17	4972.0
18	5183.0
19	5567.5
20	5952.0
21	7039.0
22	8126.0
23	8590.5
24	9055.0
25	11528.5
26	16814.0
27	19626.0
28	23662.5
29	27699.0
30	31809.5
31	35920.0
32	41775.0
33	47630.0
34	53956.5
35	60283.0
36	65607.0
37	70931.0
38	75905.0
39	80879.0
40	91628.5
41	102378.0
42	110830.0
43	119282.0
44	129929.0
45	140576.0
46	160256.5
47	179937.0
48	192422.0
49	204907.0
50	201261.5
51	197616.0
52	176719.5
53	155823.0
54	141731.5
55	127640.0
56	113846.5
57	100053.0
58	91992.5
59	83932.0
60	76915.0
61	69898.0
62	63757.5
63	57617.0
64	49181.5
65	40746.0
66	34199.5
67	27653.0
68	22332.0
69	17011.0
70	14809.5
71	12608.0
72	10818.5
73	9029.0
74	7733.0
75	5121.5
76	3806.0
77	2907.0
78	2008.0
79	1528.0
80	1048.0
81	739.5
82	431.0
83	286.0
84	141.0
85	111.5
86	82.0
87	63.5
88	45.0
89	34.5
90	24.0
91	25.5
92	27.0
93	16.5
94	6.0
95	4.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2083356.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.96986674766829
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.89626323588337	19.82959035615272
2	12.075101585133478	6.75477964601483
3	4.624382888704531	3.8803011956178928
4	2.3264157188844248	2.602781506275132
5	1.350842768747249	1.8891446119455924
6	0.8597210993880062	1.4427770754024851
7	0.6178783534506327	1.209738264859802
8	0.484666747757585	1.0844851481444329
9	0.38216178429044617	0.9620112764389441
>10	5.329073879918252	37.208686061239874
>50	0.9711588086761664	17.497251764020405
>100	0.07604025053188797	3.4937593409255383
>500	0.003845648054143337	0.7750350014124758
>1k	0.0024472305799093964	1.3696587515499052
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3734	0.17923004997705624	No Hit
GCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC	3686	0.17692607504430352	No Hit
CTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGCT	3383	0.16238223328130189	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCC	2351	0.11284677222711817	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2294	0.1101108019944743	No Hit
CCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC	2139	0.10267088294079361	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.799947776568191E-5	0.0	0.0	0.19454188338430878	0.0
2	4.799947776568191E-5	0.0	0.0	0.676168643285161	0.0
3	4.799947776568191E-5	0.0	0.0	0.9221179673565152	0.0
4	4.799947776568191E-5	0.0	0.0	1.2348345649999328	0.0
5	4.799947776568191E-5	0.0	0.0	2.0640255434020878	0.0
6	4.799947776568191E-5	0.0	0.0	2.6864347715896852	0.0
7	4.799947776568191E-5	0.0	0.0	3.308028008655266	0.0
8	4.799947776568191E-5	0.0	0.0	4.32297696601061	0.0
9	4.799947776568191E-5	0.0	0.0	4.7009728534153545	0.0
10	4.799947776568191E-5	0.0	0.0	5.708193894850424	0.0
11	4.799947776568191E-5	0.0	0.0	7.164306052350151	0.0
12	4.799947776568191E-5	0.0	0.0	8.22466251567183	0.0
13	4.799947776568191E-5	0.0	0.0	8.65027388502013	0.0
14	4.799947776568191E-5	0.0	0.0	8.83502387494024	0.0
15	4.799947776568191E-5	0.0	0.0	9.167708255334182	0.0
16	4.799947776568191E-5	0.0	0.0	9.837492968076507	0.0
17	4.799947776568191E-5	0.0	0.0	10.714587425288812	0.0
18	4.799947776568191E-5	0.0	0.0	11.625281516937095	0.0
19	4.799947776568191E-5	0.0	0.0	12.23372289709488	0.0
20	4.799947776568191E-5	0.0	0.0	12.789364851710413	0.0
21	1.4399843329704573E-4	0.0	0.0	13.497645145620815	0.0
22	1.4399843329704573E-4	0.0	0.0	14.254980905807745	0.0
23	1.4399843329704573E-4	0.0	0.0	15.013756650327645	0.0
24	1.4399843329704573E-4	0.0	0.0	15.629253953716983	0.0
25	1.9199791106272763E-4	0.0	0.0	16.173568031579816	0.0
26	1.9199791106272763E-4	0.0	0.0	16.681450505818496	0.0
27	1.9199791106272763E-4	0.0	0.0	17.18184506152573	0.0
28	1.9199791106272763E-4	0.0	0.0	17.73777501300786	0.0
29	1.9199791106272763E-4	0.0	0.0	18.32466462764885	0.0
30	1.9199791106272763E-4	0.0	0.0	18.9639216725322	0.0
31	1.9199791106272763E-4	0.0	0.0	19.552395269939463	0.0
32	1.9199791106272763E-4	0.0	0.0	20.119221102874402	0.0
33	1.9199791106272763E-4	0.0	0.0	20.68801491439773	0.0
34	1.9199791106272763E-4	0.0	0.0	21.253640760388528	0.0
35	2.3999738882840956E-4	0.0	0.0	21.85209824917105	0.0
36	3.3599634435977336E-4	0.0	0.0	22.414316132240483	0.0
37	3.3599634435977336E-4	0.0	0.0	22.983157943241576	0.0
38	3.3599634435977336E-4	0.0	0.0	23.576719485292	0.0
39	3.8399582212545526E-4	0.0	0.0	24.193704772492076	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	3090	0.0	36.99029	1
TATCGTG	55	2.752131E-9	36.81818	1
CGTAAGG	415	0.0	36.325302	2
CACGCCG	250	0.0	36.0	26
CGACACT	25	0.0021075006	36.0	27
GACCCGT	25	0.0021075006	36.0	31
TTGCGAC	25	0.0021075006	36.0	1
AATAGCG	75	1.8189894E-12	36.0	1
TATCCGA	95	0.0	35.526314	30
CGGTAGT	260	0.0	35.48077	12
CGAATAT	230	0.0	35.21739	14
CATACGA	390	0.0	35.19231	18
ATTCGCA	45	8.7044464E-7	35.0	16
TACGCGG	180	0.0	35.0	2
GTTCACG	65	3.6743586E-10	34.615387	1
CGAGGGA	1265	0.0	34.505928	4
ATACGAA	405	0.0	34.444443	19
TACGGGA	615	0.0	34.390244	4
CGACAGG	275	0.0	34.363636	2
GCTTACG	125	0.0	34.199997	1
>>END_MODULE