FastQCFastQC Report
Sat 18 Jun 2016
SRR3552407_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552407_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1355853
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30400.2242130968475196No Hit
GCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGC27080.19972666653390891TruSeq Adapter, Index 15 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTC22990.1695611544909367No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGC21350.15746544795047843No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCG19250.1419770432340379No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19190.14153451738499676No Hit
CTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGCT16370.12073580248006238TruSeq Adapter, Index 14 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGC13760.10148592804677203TruSeq Adapter, Index 15 (95% over 23bp)
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT13560.10001084188330152No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCGA351.2121382E-745.00000413
GACGTAT207.033735E-445.09
CGGATCG253.8911774E-545.032
TACTACG253.8911774E-545.01
CGCAAAC207.033735E-445.037
GCGTACG207.033735E-445.01
TCGATAG451.9286745E-840.01
CGTTCAT451.9286745E-840.017
TCGTTGA403.4588265E-739.37524
CGTAAGG2900.038.7931022
TTACGAG356.249118E-638.571431
ACGGGTA2350.038.297875
CTACGCG301.1400302E-437.4999961
AGTTGCG552.750312E-936.818181
ACGGGCC2300.036.1956565
AGTTTCG250.002107241736.01
ATATGCG751.8189894E-1236.01
TCGATGC250.002107241736.011
CCGTCTA250.002107241736.034
CCGTAAG250.002107241736.01
Produced by FastQC (version 0.11.5)