##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552407_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1355853
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.349905188836843	31.0	31.0	34.0	30.0	34.0
2	31.54361276628071	31.0	31.0	34.0	30.0	34.0
3	31.617123685237264	31.0	31.0	34.0	30.0	34.0
4	35.328836533163994	37.0	35.0	37.0	33.0	37.0
5	35.14274851329753	37.0	35.0	37.0	33.0	37.0
6	35.23298985952017	37.0	35.0	37.0	32.0	37.0
7	35.645107544844464	37.0	35.0	37.0	35.0	37.0
8	35.68439203955001	37.0	35.0	37.0	35.0	37.0
9	37.20762796556854	39.0	37.0	39.0	34.0	39.0
10	36.58531050194969	38.0	35.0	39.0	32.0	39.0
11	36.32019990367687	38.0	35.0	39.0	32.0	39.0
12	36.34315003175123	38.0	35.0	39.0	32.0	39.0
13	36.117056937588366	38.0	35.0	39.0	31.0	39.0
14	37.30640784804842	39.0	36.0	41.0	32.0	41.0
15	37.404085103621114	39.0	36.0	41.0	32.0	41.0
16	37.38755897578867	39.0	36.0	41.0	32.0	41.0
17	37.39538062017048	39.0	36.0	41.0	32.0	41.0
18	37.3360725683389	39.0	36.0	41.0	32.0	41.0
19	37.2807509368641	39.0	36.0	41.0	32.0	41.0
20	36.90669194964351	39.0	35.0	40.0	31.0	41.0
21	37.022730340236	39.0	35.0	40.0	31.0	41.0
22	37.11424468581771	39.0	35.0	40.0	31.0	41.0
23	36.933356344677485	39.0	35.0	40.0	31.0	41.0
24	36.975538646151165	39.0	35.0	40.0	31.0	41.0
25	37.005769799528416	39.0	35.0	40.0	31.0	41.0
26	36.95236209235072	39.0	35.0	40.0	31.0	41.0
27	36.74307244221903	39.0	35.0	40.0	31.0	41.0
28	36.73170764087257	39.0	35.0	40.0	30.0	41.0
29	36.78133912747178	39.0	35.0	40.0	31.0	41.0
30	36.64611724132336	39.0	35.0	40.0	30.0	41.0
31	36.57204873979701	39.0	35.0	40.0	30.0	41.0
32	36.31969689929513	38.0	35.0	40.0	30.0	41.0
33	36.26591968303349	38.0	35.0	40.0	30.0	41.0
34	36.232477267078366	38.0	35.0	40.0	30.0	41.0
35	36.03967096728038	38.0	35.0	40.0	29.0	41.0
36	36.10332093523413	38.0	35.0	40.0	30.0	41.0
37	36.12417275324095	38.0	35.0	40.0	30.0	41.0
38	36.07709685341995	38.0	35.0	40.0	30.0	41.0
39	36.068087764676555	38.0	35.0	40.0	30.0	41.0
40	35.949177381323786	38.0	35.0	40.0	29.0	41.0
41	35.836852520147836	38.0	35.0	40.0	29.0	41.0
42	35.8346126018086	38.0	35.0	40.0	29.0	41.0
43	35.795057428792056	38.0	34.0	40.0	29.0	41.0
44	35.72840787312489	38.0	34.0	40.0	29.0	41.0
45	35.648112295359454	38.0	34.0	40.0	28.0	41.0
46	35.51867127188567	38.0	34.0	40.0	28.0	41.0
47	35.42350018770471	38.0	34.0	40.0	28.0	41.0
48	35.34666147436337	38.0	34.0	40.0	27.0	41.0
49	35.29432984254193	38.0	34.0	40.0	27.0	41.0
50	35.18919897658522	37.0	34.0	40.0	27.0	41.0
51	33.9301413943842	36.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	39.0
9	76.0
10	113.0
11	84.0
12	37.0
13	36.0
14	38.0
15	44.0
16	115.0
17	267.0
18	522.0
19	903.0
20	1623.0
21	2341.0
22	3792.0
23	5355.0
24	7373.0
25	10187.0
26	12931.0
27	16139.0
28	20214.0
29	25858.0
30	32804.0
31	41684.0
32	53140.0
33	69709.0
34	99242.0
35	116837.0
36	129904.0
37	175306.0
38	257017.0
39	272089.0
40	34.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.024054967610795	19.08813123546579	24.904543486646414	10.983270310277
2	31.413582445884618	24.23994341569477	26.03490201371388	18.311572124706736
3	32.40808553729645	25.375538498642552	26.129086265251466	16.08728969880953
4	28.041387967574654	26.849444593182298	26.50309436199942	18.60607307724362
5	24.98124796714688	30.49548881774057	25.464707457224346	19.058555757888207
6	25.59606388008139	36.93298609805045	23.581464952321525	13.889485069546625
7	82.85404096166768	5.937221807968858	7.4199784194894285	3.78875881087404
8	84.89725656099887	5.1832315155109	6.186290106670856	3.733221816819375
9	79.71181241624276	7.2102949213520935	7.60930572857087	5.468586933834272
10	38.67226019339854	29.262464293695555	17.212116652763978	14.853158860141919
11	28.91751539436797	27.70492081368703	24.051574912619582	19.325988879325415
12	24.888465047464585	25.098886088683653	29.895202503516238	20.11744636033552
13	24.36333437326908	25.95760749874802	31.76022769430019	17.918830433682707
14	22.1604406967422	29.058459877287586	29.33326842954214	19.447830996428078
15	21.55329523185773	27.903319902673818	31.318734405573466	19.22465045989499
16	24.012411374979443	26.907341725098515	29.6267368217646	19.45351007815744
17	24.35913037770319	26.29606601895633	27.790107039627454	21.554696563713026
18	24.76035381416717	25.978848739502	29.020033882729173	20.240763563601657
19	24.05585266249365	28.467687868817638	27.056694199149906	20.419765269538807
20	24.390844730217804	28.20460625156267	27.858329774687963	19.546219243531564
21	24.527363954647	28.06240794540411	28.470638041144582	18.93959005880431
22	23.4639743394011	25.981135123055378	27.188198130623302	23.36669240692022
23	21.801183461628952	28.690425879501685	28.21898834165651	21.289402317212854
24	22.348661691201038	27.83701477962581	28.383239185958946	21.4310843432142
25	22.055340807594924	29.1038187768143	26.99444556305145	21.846394852539326
26	21.62645950556587	28.331537415929308	27.993005141412823	22.048997937092
27	22.020381265520673	28.741906386606807	28.128196788294897	21.109515559577623
28	20.529585434409185	27.843505158745085	29.250294832846922	22.376614573998804
29	21.737902265216068	27.44301926536284	27.547381611428378	23.27169685799272
30	22.24643822007253	28.259110685302907	27.75286111399982	21.74158998062474
31	23.687744910399577	28.329767312533143	25.63131843938834	22.351169337678936
32	22.27365355978856	28.769047972014665	26.019192346072916	22.93810612212386
33	22.317906144692675	28.645214488591314	25.29809647506035	23.738782891655656
34	22.058217225613692	27.440954144733983	27.112526210437267	23.388302419215062
35	21.144327593035527	27.231196892288473	27.05485034144557	24.56962517323043
36	22.693315573295926	30.187564581116096	25.41057179502498	21.708548050563003
37	22.31982375670519	29.0873715660916	26.340761129709488	22.25204354749372
38	21.891237471908827	29.850433638454906	25.660672654041406	22.59765623559486
39	22.48259951484416	27.931272785471585	25.798593210325897	23.787534489358357
40	23.07307650608141	26.588723113788888	27.950006379747656	22.388194000382047
41	21.195439328599782	27.75286111399982	27.47030835938704	23.581391198013353
42	21.406450404284243	28.303805796056064	27.799842608306356	22.48990119135334
43	21.67521110326857	27.530565629164816	27.23186068106203	23.562362586504584
44	21.97391605137135	27.260551106941534	26.6293617375925	24.13617110409462
45	22.781746988795984	26.57935631665085	26.55368981740646	24.08520687714671
46	22.99629827127277	27.509176879794488	26.545503089199197	22.94902175973354
47	21.021969195775647	27.870646744152943	28.37925645331758	22.72812760675383
48	20.721936670125743	28.232780397284955	27.84741413707828	23.197868795511017
49	22.404419948180223	26.568219416116644	28.092647211755256	22.934713423947876
50	21.53198023679558	25.718348523033104	28.355655074702053	24.394016165469264
51	20.83647711071923	26.163013247011293	26.82001662422106	26.180493018048416
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3348.0
1	3081.0
2	2814.0
3	3339.0
4	3864.0
5	2846.5
6	1829.0
7	1824.5
8	1820.0
9	1960.5
10	2101.0
11	2240.0
12	2379.0
13	2615.0
14	2851.0
15	2942.5
16	3034.0
17	3046.0
18	3058.0
19	3343.5
20	3629.0
21	4480.0
22	5331.0
23	5464.0
24	5597.0
25	7021.5
26	10640.0
27	12834.0
28	14344.5
29	15855.0
30	18438.5
31	21022.0
32	26229.0
33	31436.0
34	34854.0
35	38272.0
36	42672.5
37	47073.0
38	50402.5
39	53732.0
40	60185.5
41	66639.0
42	71788.0
43	76937.0
44	85564.0
45	94191.0
46	106495.0
47	118799.0
48	127137.0
49	135475.0
50	135197.5
51	134920.0
52	122321.5
53	109723.0
54	98098.0
55	86473.0
56	76445.0
57	66417.0
58	59815.0
59	53213.0
60	47465.5
61	41718.0
62	36782.5
63	31847.0
64	27659.0
65	23471.0
66	19795.5
67	16120.0
68	13474.5
69	10829.0
70	8943.5
71	7058.0
72	5760.5
73	4463.0
74	3724.5
75	2489.5
76	1993.0
77	1592.0
78	1191.0
79	895.5
80	600.0
81	440.0
82	280.0
83	197.5
84	115.0
85	74.0
86	33.0
87	25.0
88	17.0
89	11.5
90	6.0
91	7.0
92	8.0
93	5.5
94	3.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1355853.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.812692456293775
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.22533803455366	20.81006452341452
2	11.419977854835617	6.580806195781283
3	4.364644924911258	3.772715157071745
4	2.2722974286499467	2.6188402792367222
5	1.3096723320997068	1.886759306165295
6	0.8688026923098603	1.5019526867234418
7	0.6425418333595059	1.2959352164423428
8	0.4725171060700745	1.0891592046028
9	0.38887003688718413	1.0083953500468246
>10	4.935048091109874	34.861788131907986
>50	1.0159545663084806	19.05534491243081
>100	0.08018321713123902	3.699312133282022
>500	0.0010379704434199972	0.20478628687222283
>1k	0.0031139113302599917	1.6141406160220588
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3040	0.2242130968475196	No Hit
GCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGC	2708	0.19972666653390891	TruSeq Adapter, Index 15 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTC	2299	0.1695611544909367	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGC	2135	0.15746544795047843	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCG	1925	0.1419770432340379	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1919	0.14153451738499676	No Hit
CTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGCT	1637	0.12073580248006238	TruSeq Adapter, Index 14 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGC	1376	0.10148592804677203	TruSeq Adapter, Index 15 (95% over 23bp)
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	1356	0.10001084188330152	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.375430817352618E-5	0.0	0.0	0.14787738788792001	0.0
2	7.375430817352618E-5	0.0	0.0	0.6588472349141095	0.0
3	7.375430817352618E-5	0.0	0.0	0.8798151422019939	0.0
4	7.375430817352618E-5	0.0	0.0	1.1878868874428128	0.0
5	7.375430817352618E-5	0.0	0.0	2.1447752816861416	0.0
6	7.375430817352618E-5	0.0	0.0	2.6845830632081795	0.0
7	7.375430817352618E-5	0.0	0.0	3.23928921498127	0.0
8	7.375430817352618E-5	0.0	0.0	4.1691097781249145	0.0
9	7.375430817352618E-5	0.0	0.0	4.453580144750205	0.0
10	7.375430817352618E-5	0.0	0.0	5.4812726748401195	0.0
11	7.375430817352618E-5	0.0	0.0	6.782298671021121	0.0
12	7.375430817352618E-5	0.0	0.0	7.938766223182012	0.0
13	7.375430817352618E-5	0.0	0.0	8.348397650777777	0.0
14	7.375430817352618E-5	0.0	0.0	8.508739516747022	0.0
15	7.375430817352618E-5	0.0	0.0	8.831193352081678	0.0
16	7.375430817352618E-5	0.0	0.0	9.50221004784442	0.0
17	7.375430817352618E-5	0.0	0.0	10.363070332845817	0.0
18	7.375430817352618E-5	0.0	0.0	11.248490802469	0.0
19	7.375430817352618E-5	0.0	0.0	11.824438194996064	0.0
20	7.375430817352618E-5	0.0	0.0	12.37088386425372	0.0
21	1.4750861634705236E-4	0.0	0.0	13.056872684575687	0.0
22	1.4750861634705236E-4	0.0	0.0	13.794046994770081	0.0
23	2.2126292452057857E-4	0.0	0.0	14.552683808642973	0.0
24	2.950172326941047E-4	0.0	0.0	15.120518227270951	0.0
25	2.950172326941047E-4	0.0	0.0	15.646533953164539	0.0
26	3.6877154086763095E-4	0.0	0.0	16.15293103308397	0.0
27	3.6877154086763095E-4	0.0	0.0	16.624811096778192	0.0
28	3.6877154086763095E-4	0.0	0.0	17.15547334408671	0.0
29	3.6877154086763095E-4	0.0	0.0	17.719841310230535	0.0
30	3.6877154086763095E-4	0.0	0.0	18.32300404247363	0.0
31	3.6877154086763095E-4	0.0	0.0	18.928231895345586	0.0
32	3.6877154086763095E-4	0.0	0.0	19.47652142230758	0.0
33	3.6877154086763095E-4	0.0	0.0	20.051583763136563	0.0
34	3.6877154086763095E-4	0.0	0.0	20.611305207865456	0.0
35	3.6877154086763095E-4	0.0	0.0	21.22693241818988	0.0
36	3.6877154086763095E-4	0.0	0.0	21.802953565025117	0.0
37	3.6877154086763095E-4	0.0	0.0	22.36584644500547	0.0
38	3.6877154086763095E-4	0.0	0.0	22.96701781092788	0.0
39	3.6877154086763095E-4	0.0	0.0	23.59783840873605	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGCGA	35	1.2121382E-7	45.000004	13
GACGTAT	20	7.033735E-4	45.0	9
CGGATCG	25	3.8911774E-5	45.0	32
TACTACG	25	3.8911774E-5	45.0	1
CGCAAAC	20	7.033735E-4	45.0	37
GCGTACG	20	7.033735E-4	45.0	1
TCGATAG	45	1.9286745E-8	40.0	1
CGTTCAT	45	1.9286745E-8	40.0	17
TCGTTGA	40	3.4588265E-7	39.375	24
CGTAAGG	290	0.0	38.793102	2
TTACGAG	35	6.249118E-6	38.57143	1
ACGGGTA	235	0.0	38.29787	5
CTACGCG	30	1.1400302E-4	37.499996	1
AGTTGCG	55	2.750312E-9	36.81818	1
ACGGGCC	230	0.0	36.195656	5
AGTTTCG	25	0.0021072417	36.0	1
ATATGCG	75	1.8189894E-12	36.0	1
TCGATGC	25	0.0021072417	36.0	11
CCGTCTA	25	0.0021072417	36.0	34
CCGTAAG	25	0.0021072417	36.0	1
>>END_MODULE