FastQCFastQC Report
Sat 18 Jun 2016
SRR3552406_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552406_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1549130
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGCT34000.21947802960371304No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28370.1831350499958041No Hit
GCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGC25820.1666741977755256No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCC23400.1510525262566731No Hit
CCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGC18640.12032560211215326No Hit
TCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGC15950.10296101682880068No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATCGA207.034048E-445.016
GCGTAAG1200.041.2499961
ACGATTG403.459263E-739.3751
TCGTACA403.459263E-739.37534
CTTACCG403.459263E-739.3751
TAGGGCG5800.039.1810385
GCGAGAC2950.038.89830421
TACGAAT1450.038.79310612
CACGACC2950.038.13559327
GCCGATA659.094947E-1238.076929
ACACGAC2900.038.01724226
CGACCAA2900.038.01724229
CGTTTTT27300.037.9120861
CGAATAT1500.037.50000414
ACTACGG301.1401055E-437.4999962
AACACGT2900.037.24138341
TATAGCG552.750312E-936.8181841
AAACACG3050.036.1475440
ACGCATT504.8808943E-836.017
CACGAAC250.002107334336.01
Produced by FastQC (version 0.11.5)