##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552406_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1549130
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.27737504276594	31.0	31.0	34.0	30.0	34.0
2	31.473838864394853	31.0	31.0	34.0	28.0	34.0
3	31.530162736503716	31.0	31.0	34.0	28.0	34.0
4	35.250681995700816	37.0	35.0	37.0	33.0	37.0
5	35.072932549237315	37.0	35.0	37.0	32.0	37.0
6	35.17083588853098	37.0	35.0	37.0	32.0	37.0
7	35.61936312639998	37.0	35.0	37.0	35.0	37.0
8	35.66103684003279	37.0	35.0	37.0	35.0	37.0
9	37.1563251631561	39.0	37.0	39.0	34.0	39.0
10	36.498595340610535	38.0	35.0	39.0	32.0	39.0
11	36.24560237036272	38.0	35.0	39.0	32.0	39.0
12	36.23703368987754	38.0	35.0	39.0	32.0	39.0
13	36.01021928437252	38.0	35.0	39.0	31.0	39.0
14	37.1985314337725	39.0	36.0	40.0	32.0	41.0
15	37.30138142053927	39.0	36.0	40.0	32.0	41.0
16	37.268976780515516	39.0	36.0	41.0	32.0	41.0
17	37.281772349641415	39.0	36.0	41.0	32.0	41.0
18	37.23301143222325	39.0	36.0	40.0	32.0	41.0
19	37.19047594456243	39.0	36.0	40.0	32.0	41.0
20	36.80153699173084	39.0	35.0	40.0	31.0	41.0
21	36.89569693957253	39.0	35.0	40.0	31.0	41.0
22	36.98053488086862	39.0	35.0	40.0	31.0	41.0
23	36.823478984978664	39.0	35.0	40.0	31.0	41.0
24	36.88133855777114	39.0	35.0	40.0	31.0	41.0
25	36.897166796847266	39.0	35.0	40.0	31.0	41.0
26	36.83757786628624	39.0	35.0	40.0	31.0	41.0
27	36.62345639165209	39.0	35.0	40.0	30.0	41.0
28	36.60214765707203	39.0	35.0	40.0	30.0	41.0
29	36.64904301123857	39.0	35.0	40.0	30.0	41.0
30	36.52317558887892	38.0	35.0	40.0	30.0	41.0
31	36.40664631115529	38.0	35.0	40.0	30.0	41.0
32	36.152661171108946	38.0	35.0	40.0	30.0	41.0
33	36.06310961636532	38.0	35.0	40.0	29.0	41.0
34	35.97390922646905	38.0	35.0	40.0	29.0	41.0
35	35.83502223828859	38.0	35.0	40.0	29.0	41.0
36	35.89490100895341	38.0	35.0	40.0	29.0	41.0
37	35.87823746231756	38.0	35.0	40.0	29.0	41.0
38	35.84667393956608	38.0	35.0	40.0	29.0	41.0
39	35.82206464273496	38.0	35.0	40.0	29.0	41.0
40	35.71943348847417	38.0	34.0	40.0	28.0	41.0
41	35.65158895638197	38.0	34.0	40.0	28.0	41.0
42	35.61565717531776	38.0	34.0	40.0	28.0	41.0
43	35.48928237139556	38.0	34.0	40.0	28.0	41.0
44	35.40256337428105	38.0	34.0	40.0	27.0	41.0
45	35.33410559475318	38.0	34.0	40.0	27.0	41.0
46	35.231355018623354	38.0	34.0	40.0	27.0	41.0
47	35.13676644310032	38.0	34.0	40.0	26.0	41.0
48	35.0672842175931	37.0	34.0	40.0	26.0	41.0
49	34.983821241600126	37.0	34.0	40.0	26.0	41.0
50	34.854174278465976	37.0	33.0	40.0	26.0	41.0
51	33.62016486673165	36.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	25.0
9	63.0
10	90.0
11	61.0
12	40.0
13	31.0
14	34.0
15	64.0
16	156.0
17	320.0
18	647.0
19	1085.0
20	1853.0
21	3077.0
22	4451.0
23	6618.0
24	9430.0
25	12917.0
26	16901.0
27	20665.0
28	25349.0
29	31472.0
30	39340.0
31	49719.0
32	63714.0
33	82401.0
34	116964.0
35	133527.0
36	152745.0
37	202679.0
38	286373.0
39	286280.0
40	35.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.56542059091232	19.9144681208162	25.617475615345388	10.902635672926094
2	32.12925964896426	24.131157488396713	26.109041849296055	17.630541013342974
3	32.315364107595876	23.904707803735	26.935763944923924	16.8441641437452
4	28.51374642541297	26.44897458573522	26.476344787074034	18.560934201777773
5	24.833358078405297	30.57980931232369	25.379729267395245	19.20710334187576
6	25.482561179500753	36.13757399314454	24.14555266504425	14.23431216231046
7	82.93564775067296	5.670150342450278	7.2704679400695875	4.123733966807175
8	84.59341695015911	4.722457121093775	6.875342934421255	3.8087829943258473
9	78.52149270881074	7.020456643406299	9.176763731901131	5.281286915881817
10	39.52031140059259	26.084576504231404	18.38845029145391	16.00666180372209
11	30.231613873593567	27.22915442861477	23.047452441047554	19.491779256744106
12	25.7339926281203	24.838134953167263	29.66516690013104	19.7627055185814
13	24.40498860650817	25.186846810790577	31.159489519924087	19.24867506277717
14	22.1854847559598	27.474969821770927	29.951004757509054	20.38854066476022
15	21.893256214778617	26.575109900395706	31.71263870688709	19.818995177938582
16	24.534738853420954	25.649235377276273	29.276561683009174	20.539464086293595
17	24.59315874071253	26.138025859676077	27.92147850729119	21.347336892320207
18	24.76448070852672	25.73115232420778	28.89086132216147	20.613505645104027
19	24.218496833706663	27.973378606056304	27.381304345019466	20.42682021521757
20	25.132170960474586	27.441338041352243	27.734857629766385	19.691633368406784
21	24.141873180430306	28.14069832744831	28.072789242994457	19.64463924912693
22	23.036026673035835	26.095937719881483	27.728015079431685	23.140020527651004
23	22.653037511377352	27.281119079741533	27.660428756786064	22.405414652095047
24	22.247713232588616	26.92556467178352	28.496833706661157	22.329888388966708
25	22.01467920703879	28.045419041655638	26.79381330165964	23.14608844964593
26	21.43441802818356	28.25514966465048	28.100998625034695	22.20943368213126
27	22.454409894585993	27.283701174207458	28.539309160625642	21.722579770580907
28	20.607954142002285	28.187498789643218	28.418015273088766	22.78653179526573
29	22.060640488532275	26.841194735109386	27.711812436657997	23.38635233970035
30	23.561095582681894	27.339732624118053	27.450762686152874	21.648409107047183
31	22.632768069819832	29.121184148522072	25.69422837334504	22.551819408313055
32	22.348156707313137	28.125657627184292	26.107169830808257	23.419015834694314
33	23.273966678070916	27.203591693402103	26.352404252709587	23.170037375817394
34	20.604468314473284	28.031282074454694	27.592584224693862	23.77166538637816
35	22.173671673778188	26.449942871159944	27.00535139078063	24.37103406428124
36	23.31224622852827	29.117440111546482	25.463711889899493	22.106601770025758
37	22.39482806478475	29.617204495426463	26.663740292938616	21.324227146850166
38	21.343980169514502	28.683325479462667	27.641321257738216	22.33137309328462
39	22.779495587846082	26.306636628301046	28.28968517813224	22.62418260572063
40	21.79507207271178	26.693369826935122	28.129530768883182	23.38202733146992
41	21.016764248320026	27.064287696965394	26.450265632968183	25.468682421746397
42	23.013756108267224	26.503198569519665	26.36363636363636	24.11940895857675
43	22.56034032005061	25.60088565840181	28.4220820718726	23.416691949674977
44	21.662610626609773	26.48208994726072	27.56050170095473	24.294797725174774
45	22.330082046051654	25.54130382860057	27.71575013071853	24.412863994629245
46	21.875568867687022	27.219600679090846	27.0676444197711	23.837186033451033
47	20.766042875678608	27.233156675036955	28.685907573928592	23.314892875355845
48	21.20409520182296	26.252089882708358	28.81346304054534	23.730351874923343
49	22.617727369555816	25.447961113657342	28.75691517174156	23.177396345045285
50	20.619896328907192	25.12623214320296	28.93456327099727	25.31930825689258
51	20.752228670285902	24.98273224325912	27.04356638887634	27.22147269757864
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	902.0
1	1118.5
2	1335.0
3	3387.5
4	5440.0
5	4041.0
6	2642.0
7	2586.5
8	2531.0
9	2731.0
10	2931.0
11	3184.0
12	3437.0
13	3528.5
14	3620.0
15	3668.5
16	3717.0
17	4039.0
18	4361.0
19	4469.0
20	4577.0
21	5426.5
22	6276.0
23	6745.0
24	7214.0
25	9102.5
26	13805.0
27	16619.0
28	19081.5
29	21544.0
30	23555.0
31	25566.0
32	28339.0
33	31112.0
34	36270.5
35	41429.0
36	43727.5
37	46026.0
38	52202.5
39	58379.0
40	64714.0
41	71049.0
42	79319.0
43	87589.0
44	95659.0
45	103729.0
46	117164.0
47	130599.0
48	143771.0
49	156943.0
50	154109.5
51	151276.0
52	135688.5
53	120101.0
54	105777.5
55	91454.0
56	84077.0
57	76700.0
58	69732.0
59	62764.0
60	58651.0
61	54538.0
62	49067.0
63	43596.0
64	37246.0
65	30896.0
66	26673.5
67	22451.0
68	18666.5
69	14882.0
70	12912.5
71	10943.0
72	9261.0
73	7579.0
74	6202.0
75	3988.5
76	3152.0
77	2452.5
78	1753.0
79	1358.5
80	964.0
81	694.0
82	424.0
83	305.0
84	186.0
85	116.5
86	47.0
87	35.0
88	23.0
89	16.0
90	9.0
91	7.5
92	6.0
93	4.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1549130.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.08678714579091
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.08725206039135	23.130483127867542
2	11.571325521668827	7.425733180168911
3	4.337659132912308	4.175446359262596
4	2.0485016053244687	2.629193399114288
5	1.2839234337569443	2.059848896522602
6	0.8604442022736695	1.656533398151105
7	0.6333879583114713	1.4226369219333084
8	0.5244361846194426	1.3461977781947805
9	0.428519474768537	1.237483185725175
>10	5.766968669945892	42.26221173383112
>50	0.40493408796406216	8.10266939522125
>100	0.04752630244577327	2.6200493319923823
>500	0.0032776739950020235	0.7137758324992652
>1k	0.0018436916221886382	1.2177374595157369
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGCT	3400	0.21947802960371304	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2837	0.1831350499958041	No Hit
GCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGC	2582	0.1666741977755256	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCC	2340	0.1510525262566731	No Hit
CCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGC	1864	0.12032560211215326	No Hit
TCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGC	1595	0.10296101682880068	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.45523616481509E-5	0.0	0.0	0.2572411611678813	0.0
2	6.45523616481509E-5	0.0	0.0	0.7782432720301072	0.0
3	6.45523616481509E-5	0.0	0.0	1.0507833429086002	0.0
4	6.45523616481509E-5	0.0	0.0	1.3687682763873916	0.0
5	6.45523616481509E-5	0.0	0.0	2.1972978381414086	0.0
6	6.45523616481509E-5	0.0	0.0	2.824746793361435	0.0
7	6.45523616481509E-5	0.0	0.0	3.4795013975586295	0.0
8	6.45523616481509E-5	0.0	0.0	4.579086325873232	0.0
9	6.45523616481509E-5	0.0	0.0	4.973113941373545	0.0
10	6.45523616481509E-5	0.0	0.0	6.130085919193354	0.0
11	6.45523616481509E-5	0.0	0.0	7.873386997863316	0.0
12	6.45523616481509E-5	0.0	0.0	9.122991614648221	0.0
13	6.45523616481509E-5	0.0	0.0	9.649287019165596	0.0
14	6.45523616481509E-5	0.0	0.0	9.862245260242846	0.0
15	6.45523616481509E-5	0.0	0.0	10.25278704821416	0.0
16	6.45523616481509E-5	0.0	0.0	11.082349447754545	0.0
17	6.45523616481509E-5	0.0	0.0	12.1067954271107	0.0
18	6.45523616481509E-5	0.0	0.0	13.14156978433056	0.0
19	6.45523616481509E-5	0.0	0.0	13.783736678006365	0.0
20	6.45523616481509E-5	0.0	0.0	14.407506148612446	0.0
21	2.582094465926036E-4	0.0	0.0	15.175872909310387	0.0
22	3.227618082407545E-4	0.0	0.0	16.013568906418442	0.0
23	3.227618082407545E-4	0.0	0.0	16.871534345084015	0.0
24	3.227618082407545E-4	0.0	0.0	17.533066947254266	0.0
25	3.873141698889054E-4	0.0	0.0	18.14140840342644	0.0
26	4.5186653153705627E-4	0.0	0.0	18.70385313046678	0.0
27	4.5186653153705627E-4	0.0	0.0	19.258616126470987	0.0
28	4.5186653153705627E-4	0.0	0.0	19.83907096241116	0.0
29	4.5186653153705627E-4	0.0	0.0	20.439666135185554	0.0
30	4.5186653153705627E-4	0.0	0.0	21.083705047349156	0.0
31	4.5186653153705627E-4	0.0	0.0	21.7035368238947	0.0
32	4.5186653153705627E-4	0.0	0.0	22.32911376062693	0.0
33	4.5186653153705627E-4	0.0	0.0	22.94810635647105	0.0
34	4.5186653153705627E-4	0.0	0.0	23.566001562167152	0.0
35	4.5186653153705627E-4	0.0	0.0	24.180604597419197	0.0
36	4.5186653153705627E-4	0.0	0.0	24.780360589492165	0.0
37	4.5186653153705627E-4	0.0	0.0	25.384441589795564	0.0
38	4.5186653153705627E-4	0.0	0.0	26.01008307888944	0.0
39	4.5186653153705627E-4	0.0	0.0	26.665870520873007	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATCGA	20	7.034048E-4	45.0	16
GCGTAAG	120	0.0	41.249996	1
ACGATTG	40	3.459263E-7	39.375	1
TCGTACA	40	3.459263E-7	39.375	34
CTTACCG	40	3.459263E-7	39.375	1
TAGGGCG	580	0.0	39.181038	5
GCGAGAC	295	0.0	38.898304	21
TACGAAT	145	0.0	38.793106	12
CACGACC	295	0.0	38.135593	27
GCCGATA	65	9.094947E-12	38.07692	9
ACACGAC	290	0.0	38.017242	26
CGACCAA	290	0.0	38.017242	29
CGTTTTT	2730	0.0	37.912086	1
CGAATAT	150	0.0	37.500004	14
ACTACGG	30	1.1401055E-4	37.499996	2
AACACGT	290	0.0	37.241383	41
TATAGCG	55	2.750312E-9	36.818184	1
AAACACG	305	0.0	36.14754	40
ACGCATT	50	4.8808943E-8	36.0	17
CACGAAC	25	0.0021073343	36.0	1
>>END_MODULE