FastQCFastQC Report
Sat 18 Jun 2016
SRR3552405_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552405_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1692045
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC71300.4213835920439469TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGCT59600.3522364948922753TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC47230.2791296921772175TruSeq Adapter, Index 16 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC34130.2017085834005597TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCC33920.20046748165681172No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29410.1738133442077486No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21540.1273015788587183No Hit
ACTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC21040.12434657470693747TruSeq Adapter, Index 13 (95% over 21bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19960.11796376573909087No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATCCG207.0342334E-445.01
TATCGCG700.045.01
TACGTCG253.8915896E-545.01
ATATGCG750.042.01
CGTAAGG2450.041.326532
CGTTTTT27700.040.207581
TGTACCG403.4595178E-739.3751
CGCATGG2700.039.1666642
ATCGACT356.2500385E-638.57142618
ATTAGCG2350.038.297871
GCGATAT1600.037.968759
GTACGAG950.037.8947371
TCGATCA1800.037.50000417
GCGCTAT301.14014954E-437.50000441
TAGTACG900.037.5000041
AGGGCGC2950.037.372886
CGGCGTA1150.037.17391241
ACGATAG850.037.0588261
TTGCGCG1350.036.6666641
TAGTGCG1050.036.428571
Produced by FastQC (version 0.11.5)