##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552405_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1692045
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.08724767958299	31.0	31.0	34.0	28.0	34.0
2	31.271508736469773	31.0	31.0	34.0	28.0	34.0
3	31.343920522208332	31.0	31.0	34.0	28.0	34.0
4	35.08120942409924	37.0	35.0	37.0	32.0	37.0
5	34.91560271742182	35.0	35.0	37.0	32.0	37.0
6	35.01929617711113	36.0	35.0	37.0	32.0	37.0
7	35.5477064735276	37.0	35.0	37.0	35.0	37.0
8	35.62506139021125	37.0	35.0	37.0	35.0	37.0
9	37.0963106773165	39.0	37.0	39.0	34.0	39.0
10	36.38155663708707	38.0	35.0	39.0	32.0	39.0
11	36.10044059111903	38.0	35.0	39.0	31.0	39.0
12	36.16390344228434	38.0	35.0	39.0	31.0	39.0
13	35.96511794899072	38.0	35.0	39.0	31.0	39.0
14	37.07984480318195	39.0	36.0	40.0	31.0	41.0
15	37.17636824079738	39.0	36.0	40.0	32.0	41.0
16	37.151815702301064	39.0	36.0	40.0	32.0	41.0
17	37.17609283441043	39.0	36.0	40.0	32.0	41.0
18	37.1156860485389	39.0	36.0	40.0	32.0	41.0
19	37.08486240023167	39.0	36.0	40.0	31.0	41.0
20	36.720975505970586	39.0	35.0	40.0	31.0	41.0
21	36.81109958659492	39.0	35.0	40.0	31.0	41.0
22	36.91787570661537	39.0	35.0	40.0	31.0	41.0
23	36.74877618503054	39.0	35.0	40.0	31.0	41.0
24	36.79279156287215	39.0	35.0	40.0	31.0	41.0
25	36.80482552177986	39.0	35.0	40.0	31.0	41.0
26	36.76256778040773	39.0	35.0	40.0	31.0	41.0
27	36.59204867482839	39.0	35.0	40.0	30.0	41.0
28	36.564233220747674	39.0	35.0	40.0	30.0	41.0
29	36.62482203487496	39.0	35.0	40.0	30.0	41.0
30	36.46720625042478	38.0	35.0	40.0	30.0	41.0
31	36.36268361657048	38.0	35.0	40.0	30.0	41.0
32	36.126756085092296	38.0	35.0	40.0	30.0	41.0
33	36.07249275285232	38.0	35.0	40.0	29.0	41.0
34	36.031883312796054	38.0	35.0	40.0	29.0	41.0
35	35.849743357889416	38.0	35.0	40.0	29.0	41.0
36	35.90754264809742	38.0	35.0	40.0	29.0	41.0
37	35.958276523378515	38.0	35.0	40.0	29.0	41.0
38	35.905971767890335	38.0	35.0	40.0	29.0	41.0
39	35.848174841685655	38.0	35.0	40.0	29.0	41.0
40	35.767502046340375	38.0	34.0	40.0	28.0	41.0
41	35.71562694845586	38.0	34.0	40.0	28.0	41.0
42	35.74579399484056	38.0	34.0	40.0	28.0	41.0
43	35.65237272058367	38.0	34.0	40.0	28.0	41.0
44	35.52288739365679	38.0	34.0	40.0	27.0	41.0
45	35.418011341305935	38.0	34.0	40.0	27.0	41.0
46	35.273294149978284	38.0	34.0	40.0	27.0	41.0
47	35.195730610001505	38.0	34.0	40.0	27.0	41.0
48	35.161160016429825	38.0	34.0	40.0	26.0	41.0
49	35.10165391582375	38.0	34.0	40.0	26.0	41.0
50	34.96821183833763	37.0	34.0	40.0	26.0	41.0
51	33.63141524013841	36.0	31.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	20.0
9	65.0
10	123.0
11	92.0
12	56.0
13	39.0
14	44.0
15	75.0
16	146.0
17	306.0
18	664.0
19	1187.0
20	2029.0
21	3228.0
22	4737.0
23	7103.0
24	10014.0
25	13908.0
26	17943.0
27	22519.0
28	27633.0
29	34877.0
30	44302.0
31	56691.0
32	71673.0
33	91622.0
34	126097.0
35	147186.0
36	170187.0
37	225425.0
38	316807.0
39	295218.0
40	26.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.204642902523275	20.086581621647177	25.655996146674585	11.052779329154957
2	31.12086262481199	23.928027918879227	26.34977202142969	18.601337434879095
3	31.390181703205293	24.04209107913797	27.774143122671084	16.793584094985654
4	27.570188736115174	26.761640500104907	26.64503603627563	19.023134727504292
5	24.612702380846844	30.741440091723327	25.651977341028164	18.99388018640166
6	25.057548705855933	35.51944540482079	24.601118764571865	14.82188712475141
7	81.02981894689562	6.000135930190982	8.253799396588152	4.716245726325245
8	81.80349813391487	4.5972772591745485	8.076617347647373	5.522607259263199
9	76.0811325939913	7.47450570168051	10.453268086841662	5.991093617486533
10	36.56764447754049	30.003575555023655	18.595841127156785	14.83293884027907
11	25.828568389138585	26.685578693238064	27.774202222754123	19.711650694869228
12	22.70790670460892	24.466134174918515	32.68553732317994	20.140421797292625
13	22.539589668123483	25.171079965367348	33.81086200426112	18.478468362248048
14	20.560327887260684	27.976974607649325	30.786651655245578	20.676045849844417
15	19.531809142191843	27.447496963733236	33.5204441962241	19.500249697850826
16	21.765496780522977	27.30187435913348	30.59688128861821	20.33574757172534
17	21.848059596523733	26.63049741584887	29.06465253583681	22.456790451790585
18	22.344204793607737	25.6762674751558	31.913335638236575	20.06619209299989
19	20.969241361784114	27.87006255743789	30.164564181212672	20.996131899565317
20	22.198700389174046	28.311776578046093	29.907183319592562	19.582339713187295
21	21.735887638922133	29.003306649645843	30.226678368483107	19.034127342948917
22	20.43184430674125	26.64042622979885	28.66625887609372	24.261470587366173
23	19.285775496514574	27.98708072184841	29.559083830512783	23.16805995112423
24	20.6202553714588	26.690602200296095	30.034543998534318	22.65459842971079
25	19.65993812221306	28.333466308520162	28.30698947132021	23.699606097946567
26	20.01495232100801	28.034833588941193	29.539699003277097	22.410515086773696
27	20.786149304539773	26.82676879161015	29.61410600781894	22.772975896031134
28	18.053775165554107	26.98805291821435	31.216663859412723	23.74150805681882
29	21.93629602049591	25.271195506029688	29.373805070196124	23.418703403278283
30	22.010525724788643	25.583657645038993	29.23574727622492	23.170069353947444
31	21.3183455522755	27.12971581725072	27.694771711154253	23.85716691931952
32	22.178606360941938	26.33937040681542	28.548295110354633	22.933728121888013
33	20.399280160988624	25.020079253211353	29.718299454210733	24.86234113158929
34	20.165893933080977	23.742630958396497	31.194560428357406	24.896914680165125
35	21.991790998466353	23.469765875021054	29.786382749867762	24.75206037664483
36	20.49904110115275	25.104592371952283	31.055616133140667	23.340750393754302
37	20.20401348663895	24.83562789405719	31.395855311176714	23.564503308127147
38	20.312166638594128	25.92602442606432	31.5499883277336	22.211820607607955
39	21.394230058893232	23.52549725332364	31.736508189794005	23.34376449798912
40	21.57318511032508	22.426472109193313	31.899092518224986	24.101250262256617
41	19.36402400645373	23.80663634832407	31.082152070423657	25.74718757479854
42	21.163917035303434	23.31870606278202	32.328395521395706	23.18898138051884
43	21.665972240690998	22.358093313121106	32.27242774276098	23.703506703426918
44	21.132771291543666	23.285137215617787	29.861203454990854	25.720888037847693
45	22.635568202973325	22.010230224373466	29.589638573442194	25.764562999211016
46	22.108690962710803	22.865999426729193	30.86986457215972	24.155445038400277
47	18.925619590495522	23.47868998755943	33.15662408505684	24.439066336888203
48	19.197539072542398	22.82616597076319	33.08162607968464	24.894668877009774
49	21.661598834546364	21.20144558803105	33.599933807907	23.537021769515587
50	20.029727341766915	20.658611325348915	32.55782204373997	26.7538392891442
51	19.654500914573784	20.24780664816834	30.429923554042592	29.667768883215277
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2469.0
1	3152.5
2	3836.0
3	4890.0
4	5944.0
5	4309.5
6	2675.0
7	2639.0
8	2603.0
9	2825.0
10	3047.0
11	3222.0
12	3397.0
13	3539.5
14	3682.0
15	3860.5
16	4039.0
17	4275.5
18	4512.0
19	4627.5
20	4743.0
21	5642.0
22	6541.0
23	7129.0
24	7717.0
25	9149.5
26	12181.5
27	13781.0
28	17125.0
29	20469.0
30	25488.5
31	30508.0
32	33389.0
33	36270.0
34	40250.0
35	44230.0
36	50205.5
37	56181.0
38	58916.5
39	61652.0
40	70242.0
41	78832.0
42	87752.5
43	96673.0
44	111143.5
45	125614.0
46	145303.5
47	164993.0
48	187309.0
49	209625.0
50	204664.5
51	199704.0
52	171143.0
53	142582.0
54	121288.0
55	99994.0
56	86409.0
57	72824.0
58	62962.0
59	53100.0
60	45819.0
61	38538.0
62	33102.5
63	27667.0
64	23486.5
65	19306.0
66	15826.5
67	12347.0
68	9969.0
69	7591.0
70	6205.0
71	4819.0
72	4292.0
73	3765.0
74	3078.0
75	1822.5
76	1254.0
77	985.5
78	717.0
79	538.5
80	360.0
81	301.0
82	242.0
83	178.0
84	114.0
85	90.0
86	66.0
87	45.5
88	25.0
89	22.0
90	19.0
91	10.5
92	2.0
93	2.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1692045.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.386759409844846
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.40288324413716	21.15558079913595
2	13.464455123887697	8.452112271162315
3	5.901438502649482	5.556810913639628
4	3.010049361840826	3.779027805274201
5	1.7840037495348242	2.799704823645531
6	1.1483603180724737	2.162598541149218
7	0.807119365958555	1.7733002948057377
8	0.5689995875212117	1.428724252626338
9	0.4669458271879536	1.3190324701841358
>10	4.923197872928338	35.37894836204044
>50	0.44699898237076746	8.90565432411484
>100	0.06826172281223805	3.9088798987595994
>500	0.004601899641170594	0.9765260916093357
>1k	0.002300949820585297	1.6153063169477733
>5k	3.8349163676421614E-4	0.7877928349048567
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC	7130	0.4213835920439469	TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGCT	5960	0.3522364948922753	TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC	4723	0.2791296921772175	TruSeq Adapter, Index 16 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC	3413	0.2017085834005597	TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCC	3392	0.20046748165681172	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2941	0.1738133442077486	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2154	0.1273015788587183	No Hit
ACTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC	2104	0.12434657470693747	TruSeq Adapter, Index 13 (95% over 21bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1996	0.11796376573909087	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1820016607123333E-4	0.0	0.0	0.4104500766823577	0.0
2	1.1820016607123333E-4	0.0	0.0	1.5722395089965102	0.0
3	1.1820016607123333E-4	0.0	0.0	2.1104048651188356	0.0
4	1.1820016607123333E-4	0.0	0.0	2.757550774358838	0.0
5	1.1820016607123333E-4	0.0	0.0	4.414835302843601	0.0
6	1.1820016607123333E-4	0.0	0.0	5.37172474727327	0.0
7	1.1820016607123333E-4	0.0	0.0	6.449355661344704	0.0
8	1.1820016607123333E-4	0.0	0.0	8.269165418177412	0.0
9	1.1820016607123333E-4	0.0	0.0	8.890307290881744	0.0
10	1.1820016607123333E-4	0.0	0.0	10.61360661211729	0.0
11	1.1820016607123333E-4	0.0	0.0	13.143622066789003	0.0
12	1.1820016607123333E-4	0.0	0.0	14.937250486836934	0.0
13	1.1820016607123333E-4	0.0	0.0	15.664949809254482	0.0
14	1.1820016607123333E-4	0.0	0.0	15.928122479012083	0.0
15	1.1820016607123333E-4	0.0	0.0	16.475211947672786	0.0
16	1.1820016607123333E-4	0.0	0.0	17.615134349263762	0.0
17	1.1820016607123333E-4	0.0	0.0	19.132410781037148	0.0
18	1.1820016607123333E-4	0.0	0.0	20.727049221504156	0.0
19	1.1820016607123333E-4	0.0	0.0	21.745225452041762	0.0
20	1.1820016607123333E-4	0.0	0.0	22.673096755700943	0.0
21	5.910008303561667E-4	0.0	0.0	23.811305254883884	0.0
22	5.910008303561667E-4	0.0	0.0	25.045787789331843	0.0
23	5.910008303561667E-4	0.0	0.0	26.27967932294945	0.0
24	6.501009133917834E-4	0.0	0.0	27.215292737486298	0.0
25	7.683010794630167E-4	0.0	0.0	28.03743399259476	0.0
26	7.683010794630167E-4	0.0	0.0	28.804434870230992	0.0
27	7.683010794630167E-4	0.0	0.0	29.553351122458327	0.0
28	7.683010794630167E-4	0.0	0.0	30.368991368432873	0.0
29	7.683010794630167E-4	0.0	0.0	31.17517560112172	0.0
30	7.683010794630167E-4	0.0	0.0	32.101392102455904	0.0
31	7.683010794630167E-4	0.0	0.0	32.944750287374156	0.0
32	7.683010794630167E-4	0.0	0.0	33.75347582363353	0.0
33	7.683010794630167E-4	0.0	0.0	34.571598273095574	0.0
34	8.8650124553425E-4	0.0	0.0	35.35934327987731	0.0
35	8.8650124553425E-4	0.0	0.0	36.18674444237594	0.0
36	8.8650124553425E-4	0.0	0.0	36.96976144251483	0.0
37	8.8650124553425E-4	0.0	0.0	37.73569851865642	0.0
38	9.456013285698666E-4	0.0	0.0	38.53810034603099	0.0
39	0.0010638014946411	0.0	0.0	39.32064454550559	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATCCG	20	7.0342334E-4	45.0	1
TATCGCG	70	0.0	45.0	1
TACGTCG	25	3.8915896E-5	45.0	1
ATATGCG	75	0.0	42.0	1
CGTAAGG	245	0.0	41.32653	2
CGTTTTT	2770	0.0	40.20758	1
TGTACCG	40	3.4595178E-7	39.375	1
CGCATGG	270	0.0	39.166664	2
ATCGACT	35	6.2500385E-6	38.571426	18
ATTAGCG	235	0.0	38.29787	1
GCGATAT	160	0.0	37.96875	9
GTACGAG	95	0.0	37.894737	1
TCGATCA	180	0.0	37.500004	17
GCGCTAT	30	1.14014954E-4	37.500004	41
TAGTACG	90	0.0	37.500004	1
AGGGCGC	295	0.0	37.37288	6
CGGCGTA	115	0.0	37.173912	41
ACGATAG	85	0.0	37.058826	1
TTGCGCG	135	0.0	36.666664	1
TAGTGCG	105	0.0	36.42857	1
>>END_MODULE