FastQCFastQC Report
Sat 18 Jun 2016
SRR3552404_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552404_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences832053
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA224042.6926169366614867No Hit
CTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGCT40550.48734876263891846Illumina Single End Adapter 1 (95% over 21bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30390.36524115651286637No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29670.3565878615905477No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCC26300.31608563396802847No Hit
CCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGC20900.2511859220506386TruSeq Adapter, Index 15 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGC18020.21657274236136398TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCG15340.1843632557060668No Hit
GAATCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTC13230.1590042941976052No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGC12840.15431709278134925No Hit
TCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGC11260.13532791781292777TruSeq Adapter, Index 15 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGC11110.13352514803744472No Hit
AAAACTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTC10560.12691499219400687No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10530.12655443823891024No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTC9480.11393504981052889No Hit
CGCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTG8930.10732489396709105No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCG8400.10095510742705092No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACCG253.8898732E-545.00000423
CTCAACG253.8898732E-545.0000041
AGCTTCG253.8898732E-545.0000041
CGACCCA253.8898732E-545.00000433
ATCCGCA253.8898732E-545.00000412
TGTACCG253.8898732E-545.0000041
TATCGTG253.8898732E-545.0000041
TCACGAC207.032162E-445.025
GTCGAAG453.8380676E-1045.01
TAGACCG207.032162E-445.01
TAGCGCG351.2113924E-745.01
TCGATAG207.032162E-445.01
TTATCCG207.032162E-445.01
GTATGCG351.2113924E-745.01
CGGTGAC207.032162E-445.033
CCGACGG207.032162E-445.02
CTACACG207.032162E-445.042
TATCCGG207.032162E-445.02
TCGAGTG302.1647775E-644.9999961
ACCCGCG302.1647775E-644.99999629
Produced by FastQC (version 0.11.5)