##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552404_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	832053
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.324064692994316	31.0	31.0	34.0	30.0	34.0
2	31.5338049379066	31.0	31.0	34.0	30.0	34.0
3	31.580014734638297	31.0	31.0	34.0	30.0	34.0
4	35.31733675619221	37.0	35.0	37.0	33.0	37.0
5	35.143286545448426	37.0	35.0	37.0	33.0	37.0
6	35.261073513345906	37.0	35.0	37.0	32.0	37.0
7	35.575855143843	37.0	35.0	37.0	35.0	37.0
8	35.58155670371959	37.0	35.0	37.0	35.0	37.0
9	37.03596165148134	39.0	37.0	39.0	33.0	39.0
10	36.60392907663334	38.0	35.0	39.0	32.0	39.0
11	36.37911887824453	38.0	35.0	39.0	32.0	39.0
12	36.437229359187455	38.0	35.0	39.0	32.0	39.0
13	36.22274782976565	38.0	35.0	39.0	32.0	39.0
14	37.42331438021376	39.0	36.0	40.0	32.0	41.0
15	37.5101129375172	39.0	36.0	41.0	32.0	41.0
16	37.476417968566906	39.0	36.0	41.0	32.0	41.0
17	37.46974050931852	39.0	36.0	40.0	32.0	41.0
18	37.3390613338333	39.0	36.0	40.0	32.0	41.0
19	37.22717423048772	39.0	35.0	40.0	32.0	41.0
20	36.81788299543418	39.0	35.0	40.0	31.0	41.0
21	36.92329815528578	39.0	35.0	40.0	31.0	41.0
22	37.019515583742866	39.0	35.0	40.0	31.0	41.0
23	36.810282518060745	39.0	35.0	40.0	31.0	41.0
24	36.87450799408211	39.0	35.0	40.0	31.0	41.0
25	36.90319126305656	39.0	35.0	40.0	31.0	41.0
26	36.84212183598881	39.0	35.0	40.0	31.0	41.0
27	36.62546856991081	39.0	35.0	40.0	31.0	41.0
28	36.59742227959036	38.0	35.0	40.0	31.0	41.0
29	36.61467118080218	38.0	35.0	40.0	31.0	41.0
30	36.5135369982441	38.0	35.0	40.0	30.0	41.0
31	36.4023698009622	38.0	35.0	40.0	30.0	41.0
32	36.085743336061526	38.0	35.0	40.0	30.0	41.0
33	36.019996322349655	38.0	35.0	40.0	30.0	41.0
34	35.97220008821554	38.0	35.0	40.0	30.0	41.0
35	35.76410396933849	38.0	35.0	40.0	29.0	41.0
36	35.863286353152986	38.0	35.0	40.0	29.0	41.0
37	35.835979198440484	38.0	35.0	40.0	29.0	41.0
38	35.72238306934774	38.0	35.0	40.0	29.0	41.0
39	35.67286939654085	38.0	34.0	40.0	28.0	41.0
40	35.597930660667046	38.0	34.0	40.0	28.0	41.0
41	35.55338902690093	38.0	34.0	40.0	28.0	41.0
42	35.54940971308318	38.0	34.0	40.0	28.0	41.0
43	35.44817818095722	38.0	34.0	40.0	27.0	41.0
44	35.35172158504326	38.0	34.0	40.0	27.0	41.0
45	35.24758759357877	38.0	34.0	40.0	27.0	41.0
46	35.084864786257604	38.0	34.0	40.0	26.0	41.0
47	34.96490848539696	38.0	34.0	40.0	26.0	41.0
48	34.891226880979936	37.0	34.0	40.0	26.0	41.0
49	34.8006653422318	37.0	33.0	40.0	26.0	41.0
50	34.66931914192966	37.0	33.0	40.0	25.0	41.0
51	33.43374760982774	36.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	14.0
9	41.0
10	58.0
11	48.0
12	34.0
13	17.0
14	19.0
15	33.0
16	73.0
17	155.0
18	387.0
19	701.0
20	1242.0
21	1913.0
22	2712.0
23	3913.0
24	5336.0
25	6964.0
26	8654.0
27	10337.0
28	12926.0
29	15877.0
30	20486.0
31	26154.0
32	33652.0
33	43805.0
34	61976.0
35	82326.0
36	80272.0
37	105706.0
38	154315.0
39	151885.0
40	21.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.334562822320216	22.759487676866737	23.648012806876483	11.257936693936564
2	30.73169617800789	27.52769354836771	24.752269386685704	16.988340886938694
3	29.077234262721248	28.113353356096304	26.618616842917458	16.19079553826499
4	25.394415980712765	30.08642478303666	27.519881546007284	16.99927769024329
5	22.999256057005983	33.80289476752082	25.678893051283993	17.518956124189206
6	23.09564414766848	38.54189576865897	24.493391646926337	13.869068436746218
7	76.21449595158	10.393208124963193	8.728650698933842	4.6636452245229565
8	77.89227368929623	9.68508015715345	7.904784911538087	4.517861242012228
9	72.58395799306054	12.02868086528142	9.754907439790495	5.632453701867549
10	34.538785389873	35.02517267529833	17.54479582430446	12.891246110524209
11	24.960549388079844	30.206008511477034	26.88458547712706	17.94885662331606
12	22.521642251154674	28.327161851468595	30.663431295842937	18.487764601533797
13	22.255433247641676	29.257391055617855	32.0917056966323	16.395470000108165
14	19.78107163846534	32.405387637566356	29.549079205291008	18.264461518677297
15	19.19697423120883	31.580079634350216	31.83631331177221	17.386632822668748
16	21.01404598024405	30.962090155314627	29.772141918844113	18.251721945597215
17	20.92030195191893	30.12656645670408	28.139313240863263	20.81381835051373
18	21.05310599204618	29.544872742481548	30.276436717372572	19.1255845480997
19	20.33920916095489	32.45081743590853	28.83890809840238	18.371065304734195
20	21.592975447477507	31.974165107270814	28.219115849591315	18.21374359566037
21	20.579217910397535	33.20725963370122	28.61812889323156	17.59539356266969
22	20.138741161921175	30.044239970290356	27.652925955437937	22.164092912350537
23	18.596531711321273	32.43567416979447	27.786330918823683	21.181463200060573
24	19.13183414998804	31.313269707578723	28.72833821883943	20.82655792359381
25	18.12697027713379	34.06189269193188	26.541939035133577	21.26919799580075
26	18.85757277481122	34.09133793159811	27.400658371522006	19.650430922068665
27	20.23068242047081	32.99477316949761	27.603049324982905	19.17149508504867
28	18.191269065792685	31.58032000365361	29.466271980270488	20.762138950283216
29	19.244447168629883	31.7306710029289	28.317426894680985	20.707454933760232
30	21.74993660259623	32.37558184394504	26.64710060537009	19.227380948088644
31	22.33511567171803	33.34342884407604	24.61994608516525	19.701509399040688
32	20.79999711556836	33.55038681430149	25.533709992031756	20.11590607809839
33	21.29756157360168	32.024041737725845	25.179886377430282	21.498510311242192
34	20.69026852856729	31.47972545018166	26.145329684527308	21.684676336723744
35	20.177440619768213	32.50490052917302	26.072377600946094	21.245281250112676
36	23.029903143189195	32.53554761535623	24.969082498350463	19.465466743104106
37	22.579571253273528	31.65086839420085	26.93842820108815	18.83113215143747
38	20.419492508289736	32.97686565639448	26.22621395512065	20.377427880195132
39	21.138076540797282	31.375405172507037	26.956335714191283	20.530182572504398
40	21.965908421699098	31.625028694085593	26.39423209819567	20.01483078601964
41	20.740866266932514	32.35166509825696	24.415031254018675	22.49243738079185
42	21.05947577858622	31.326970757872395	26.07490147863177	21.538651984909617
43	21.527354627649924	29.564943579315262	27.34465232383033	21.563049469204483
44	20.833408448740645	30.099404725420136	26.730869307604205	22.336317518235017
45	21.986339812487905	29.4120687023543	26.424037891816987	22.17755359334081
46	22.1143364665472	31.52034786245588	26.543501435605666	19.821814235391255
47	19.903780167849884	31.131189960254936	27.875027191777445	21.090002680117735
48	20.23729257631425	29.99508444774552	27.599804339387035	22.1678186365532
49	21.19624591221953	28.663558691573733	28.411050738354408	21.729144657852327
50	20.33620454466242	28.039920533908298	27.880315316452197	23.743559604977087
51	19.7530686146195	27.82935702413188	26.19520631498234	26.222368046266283
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	24320.0
1	16820.0
2	9320.0
3	8098.5
4	6877.0
5	4817.5
6	2758.0
7	2493.0
8	2228.0
9	2271.0
10	2314.0
11	2379.5
12	2445.0
13	2562.0
14	2679.0
15	2737.5
16	2796.0
17	2918.0
18	3040.0
19	2947.5
20	2855.0
21	3082.0
22	3309.0
23	4059.5
24	4810.0
25	5633.5
26	7340.0
27	8223.0
28	9551.0
29	10879.0
30	12569.0
31	14259.0
32	16615.0
33	18971.0
34	21576.5
35	24182.0
36	26071.0
37	27960.0
38	30461.5
39	32963.0
40	36906.5
41	40850.0
42	45896.5
43	50943.0
44	57181.5
45	63420.0
46	71732.5
47	80045.0
48	85946.5
49	91848.0
50	89362.0
51	86876.0
52	74363.0
53	61850.0
54	52715.0
55	43580.0
56	36674.0
57	29768.0
58	26167.0
59	22566.0
60	19280.5
61	15995.0
62	14328.5
63	12662.0
64	10361.5
65	8061.0
66	6313.5
67	4566.0
68	3633.5
69	2701.0
70	2003.0
71	1305.0
72	991.0
73	677.0
74	540.5
75	276.5
76	149.0
77	103.5
78	58.0
79	48.5
80	39.0
81	32.0
82	25.0
83	17.0
84	9.0
85	7.0
86	5.0
87	3.0
88	1.0
89	1.5
90	2.0
91	2.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	832053.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.495270805595844
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.0937141272984	23.427047639477724
2	11.833364963673247	7.690567980720241
3	4.691595579292866	4.5736400657837395
4	2.306378160813805	2.997855316630267
5	1.356909638211329	2.2046573076200113
6	0.907823705735779	1.7699986296974217
7	0.6568927871499546	1.4942136306076284
8	0.5070825627476857	1.3182228157825278
9	0.4229397787996059	1.2369188381896798
>10	4.755922922574736	35.34577316439583
>50	0.40778255331614	8.377728323585004
>100	0.05097281743752692	2.986834773374272
>500	0.0033732011539539834	0.7945730737846086
>1k	0.00487240166682242	3.0533333782618235
>5k	0.0	0.0
>10k+	3.7480012821710924E-4	2.728635062089266
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	22404	2.6926169366614867	No Hit
CTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGCT	4055	0.48734876263891846	Illumina Single End Adapter 1 (95% over 21bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3039	0.36524115651286637	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2967	0.3565878615905477	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCC	2630	0.31608563396802847	No Hit
CCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGC	2090	0.2511859220506386	TruSeq Adapter, Index 15 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGC	1802	0.21657274236136398	TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCG	1534	0.1843632557060668	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTC	1323	0.1590042941976052	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGC	1284	0.15431709278134925	No Hit
TCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGC	1126	0.13532791781292777	TruSeq Adapter, Index 15 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGC	1111	0.13352514803744472	No Hit
AAAACTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTC	1056	0.12691499219400687	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1053	0.12655443823891024	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTC	948	0.11393504981052889	No Hit
CGCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTG	893	0.10732489396709105	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCG	840	0.10095510742705092	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5776074360647699	0.0
2	0.0	0.0	0.0	1.463127949782045	0.0
3	0.0	0.0	0.0	1.8531271445448787	0.0
4	0.0	0.0	0.0	2.2974498018756018	0.0
5	0.0	0.0	0.0	3.623447064069236	0.0
6	0.0	0.0	0.0	4.466422211085111	0.0
7	0.0	0.0	0.0	5.350620693633699	0.0
8	0.0	0.0	0.0	6.6593113659826955	0.0
9	0.0	0.0	0.0	7.121781905719948	0.0
10	0.0	0.0	0.0	8.656179353959423	0.0
11	0.0	0.0	0.0	10.97442110057893	0.0
12	0.0	0.0	0.0	12.790411187748857	0.0
13	0.0	0.0	0.0	13.451907510699439	0.0
14	0.0	0.0	0.0	13.733259780326494	0.0
15	0.0	0.0	0.0	14.205104722896257	0.0
16	0.0	0.0	0.0	15.179081140263902	0.0
17	0.0	0.0	0.0	16.3918644605572	0.0
18	0.0	0.0	0.0	17.67471543279094	0.0
19	0.0	0.0	0.0	18.47466447449862	0.0
20	0.0	0.0	0.0	19.179186902757397	0.0
21	1.2018465169887015E-4	0.0	0.0	20.040309932179802	0.0
22	1.2018465169887015E-4	0.0	0.0	20.94121408131453	0.0
23	1.2018465169887015E-4	0.0	0.0	21.83070068853787	0.0
24	1.2018465169887015E-4	0.0	0.0	22.534261639583054	0.0
25	3.605539550966104E-4	0.0	0.0	23.137348221807986	0.0
26	3.605539550966104E-4	0.0	0.0	23.709066609939512	0.0
27	7.211079101932208E-4	0.0	0.0	24.273333549665708	0.0
28	7.211079101932208E-4	0.0	0.0	24.880266040745	0.0
29	7.211079101932208E-4	0.0	0.0	25.52613835897473	0.0
30	7.211079101932208E-4	0.0	0.0	26.215878075074546	0.0
31	7.211079101932208E-4	0.0	0.0	26.87593218220474	0.0
32	7.211079101932208E-4	0.0	0.0	27.482624303980636	0.0
33	7.211079101932208E-4	0.0	0.0	28.084148485733483	0.0
34	7.211079101932208E-4	0.0	0.0	28.659352228764273	0.0
35	7.211079101932208E-4	0.0	0.0	29.294888666947898	0.0
36	7.211079101932208E-4	0.0	0.0	29.916603870186155	0.0
37	7.211079101932208E-4	0.0	0.0	30.510436234230273	0.0
38	7.211079101932208E-4	0.0	0.0	31.101143797330217	0.0
39	7.211079101932208E-4	0.0	0.0	31.739564667154617	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCACCG	25	3.8898732E-5	45.000004	23
CTCAACG	25	3.8898732E-5	45.000004	1
AGCTTCG	25	3.8898732E-5	45.000004	1
CGACCCA	25	3.8898732E-5	45.000004	33
ATCCGCA	25	3.8898732E-5	45.000004	12
TGTACCG	25	3.8898732E-5	45.000004	1
TATCGTG	25	3.8898732E-5	45.000004	1
TCACGAC	20	7.032162E-4	45.0	25
GTCGAAG	45	3.8380676E-10	45.0	1
TAGACCG	20	7.032162E-4	45.0	1
TAGCGCG	35	1.2113924E-7	45.0	1
TCGATAG	20	7.032162E-4	45.0	1
TTATCCG	20	7.032162E-4	45.0	1
GTATGCG	35	1.2113924E-7	45.0	1
CGGTGAC	20	7.032162E-4	45.0	33
CCGACGG	20	7.032162E-4	45.0	2
CTACACG	20	7.032162E-4	45.0	42
TATCCGG	20	7.032162E-4	45.0	2
TCGAGTG	30	2.1647775E-6	44.999996	1
ACCCGCG	30	2.1647775E-6	44.999996	29
>>END_MODULE