##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552403_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1659954
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.24191453498109	31.0	31.0	34.0	30.0	34.0
2	31.434168055259363	31.0	31.0	34.0	28.0	34.0
3	31.510342455272855	31.0	31.0	34.0	28.0	34.0
4	35.22839307595271	37.0	35.0	37.0	33.0	37.0
5	35.05504007942389	37.0	35.0	37.0	32.0	37.0
6	35.15506755006464	37.0	35.0	37.0	32.0	37.0
7	35.59308872414537	37.0	35.0	37.0	35.0	37.0
8	35.6397394144657	37.0	35.0	37.0	35.0	37.0
9	37.12981805519912	39.0	37.0	39.0	34.0	39.0
10	36.505898958645844	38.0	35.0	39.0	32.0	39.0
11	36.25129190326961	38.0	35.0	39.0	32.0	39.0
12	36.275781136103774	38.0	35.0	39.0	32.0	39.0
13	36.05677085027657	38.0	35.0	39.0	31.0	39.0
14	37.23629028274277	39.0	36.0	40.0	32.0	41.0
15	37.34413845202939	39.0	36.0	40.0	32.0	41.0
16	37.32402584649936	39.0	36.0	40.0	32.0	41.0
17	37.33342008272518	39.0	36.0	40.0	32.0	41.0
18	37.297535353389314	39.0	36.0	40.0	32.0	41.0
19	37.243248306880794	39.0	36.0	40.0	32.0	41.0
20	36.86678606756573	39.0	35.0	40.0	31.0	41.0
21	36.96603158882716	39.0	35.0	40.0	31.0	41.0
22	37.05035561226396	39.0	35.0	40.0	31.0	41.0
23	36.88943970736538	39.0	35.0	40.0	31.0	41.0
24	36.934827712093224	39.0	35.0	40.0	31.0	41.0
25	36.95789341150417	39.0	35.0	40.0	31.0	41.0
26	36.92503346478276	39.0	35.0	40.0	31.0	41.0
27	36.7329275389559	39.0	35.0	40.0	31.0	41.0
28	36.68597322576409	39.0	35.0	40.0	30.0	41.0
29	36.725761075306906	39.0	35.0	40.0	30.0	41.0
30	36.59503275391969	39.0	35.0	40.0	30.0	41.0
31	36.47473664932884	38.0	35.0	40.0	30.0	41.0
32	36.21236733066097	38.0	35.0	40.0	30.0	41.0
33	36.16184303902397	38.0	35.0	40.0	30.0	41.0
34	36.1315879837634	38.0	35.0	40.0	30.0	41.0
35	35.95419993566087	38.0	35.0	40.0	29.0	41.0
36	36.035958225348416	38.0	35.0	40.0	30.0	41.0
37	36.01038462511612	38.0	35.0	40.0	30.0	41.0
38	35.92955648168564	38.0	35.0	40.0	29.0	41.0
39	35.8871679576663	38.0	35.0	40.0	29.0	41.0
40	35.78861522668701	38.0	34.0	40.0	29.0	41.0
41	35.7352276026926	38.0	34.0	40.0	29.0	41.0
42	35.80125593841757	38.0	34.0	40.0	29.0	41.0
43	35.7296352790499	38.0	34.0	40.0	29.0	41.0
44	35.622401584622224	38.0	34.0	40.0	28.0	41.0
45	35.52033490084665	38.0	34.0	40.0	28.0	41.0
46	35.40538051054427	38.0	34.0	40.0	28.0	41.0
47	35.306646449238954	38.0	34.0	40.0	27.0	41.0
48	35.254444400266514	38.0	34.0	40.0	27.0	41.0
49	35.18132189205243	37.0	34.0	40.0	27.0	41.0
50	35.06623617280961	37.0	34.0	40.0	27.0	41.0
51	33.80519520420445	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	22.0
9	87.0
10	105.0
11	89.0
12	42.0
13	28.0
14	44.0
15	60.0
16	134.0
17	298.0
18	639.0
19	1137.0
20	1841.0
21	2868.0
22	4373.0
23	6345.0
24	8870.0
25	12249.0
26	15974.0
27	20470.0
28	25739.0
29	32286.0
30	41940.0
31	52883.0
32	68133.0
33	88146.0
34	124540.0
35	146075.0
36	165090.0
37	220255.0
38	313852.0
39	305305.0
40	31.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.004986885178745	20.743466385213083	25.397872471164863	10.853674258443306
2	30.663560556497348	24.94099234075161	26.209400983400744	18.186046119350298
3	31.49147506497168	24.680262224133923	27.136053167738382	16.692209543156014
4	27.882158180286925	26.764958547044078	26.70869192760763	18.64419134506137
5	24.782734943257463	30.498616226714713	25.26292897273057	19.45571985729725
6	25.495826992796182	36.14027858603311	23.901084005942334	14.462810415228375
7	82.13673390949387	6.237100546159713	7.0986906866093875	4.527474857737022
8	83.43333610449446	5.149178832666448	6.685727435820511	4.73175762701858
9	77.8481210925122	7.5865957731358815	8.988984032087636	5.576299102264279
10	37.57387252899779	29.37539233014891	17.860856385176938	15.18987875567636
11	29.116168279361958	26.83833407431772	24.277359493094387	19.768138153225934
12	24.77321660720719	24.63851407930581	30.32668375147745	20.26158556200955
13	24.015304038545647	25.618721964584562	31.448883523278354	18.917090473591436
14	21.14136897769456	28.788147141426812	29.429008273723245	20.641475607155378
15	20.447494328156083	27.7648657733889	31.81003810948978	19.977601788965234
16	22.871416918782085	26.34103113700741	29.78612660350829	21.001425340702212
17	22.840512447935303	26.51109609061456	27.45829101288349	23.190100448566646
18	23.199919997783073	26.218497621018415	29.463587545197033	21.11799483600148
19	22.616831550753815	28.597780420421287	27.855530936399443	20.92985709242545
20	24.013557002181987	28.270060495652288	27.56678799532999	20.149594506835733
21	23.45426439527842	28.4799458298242	28.154695853017614	19.911093921879765
22	21.47493243788683	26.888877643597354	27.078340725104432	24.557849193411382
23	20.899494805277737	28.409160735779427	27.707334058654638	22.984010400288202
24	20.646355260446978	27.295635903163586	29.39262172325257	22.66538711313687
25	21.038052861705804	28.72091636274258	27.074485196577736	23.166545578973874
26	20.25983852564589	28.51422388813184	27.65757364360699	23.568363942615278
27	21.768193576448507	27.65528442354427	27.510340647993857	23.066181352013366
28	19.646689004635068	27.67299575771377	28.913150605378224	23.76716463227294
29	21.0779334849038	26.26084819217882	28.020415023548846	24.640803299368535
30	22.819367283671717	26.05951731192551	27.48624359470202	23.63487180970075
31	22.527672453574016	28.349942227314735	25.43950013072652	23.682885188384738
32	21.66180508616504	28.20770937025966	26.354103788418232	23.776381755157068
33	21.950246814068343	26.64073823732465	26.734054076197296	24.674960872409716
34	20.782262641012945	25.728484042328887	28.85098020788528	24.638273108772893
35	20.576112350101265	25.664205152672903	28.194636718848837	25.565045778376994
36	22.501225937586224	27.183464120090072	27.18864498654782	23.126664955775883
37	21.426557603403467	27.0406288367027	29.08936030757479	22.44345325231904
38	21.50083677017556	28.500006626689654	28.146804068064533	21.85235253507025
39	23.10443542411416	25.12726256269752	29.318884740179545	22.44941727300877
40	22.13838455764437	25.44594609248208	30.042097552100845	22.37357179777271
41	19.228002703689377	27.121052752064212	28.61579296775694	25.03515157648947
42	20.956122880513554	26.270487013495554	28.59175615709833	24.181633948892557
43	21.751867822843284	24.524113318802808	29.53624016087193	24.187778697481978
44	22.012838909993892	24.97189681159839	28.127646910697525	24.88761736771019
45	22.697375951381783	24.238262024128378	28.04095776148014	25.023404263009695
46	22.499358415956106	25.827703659258027	28.045656686872043	23.62728123791382
47	20.25694687925087	26.212172144529305	29.935769304450606	23.595111671769217
48	20.314418351351904	25.559985397185702	29.965047224200188	24.160549027262203
49	22.186397936328355	24.301697516919145	30.544219900069518	22.967684646682983
50	20.71111609116879	23.584689696220497	30.217162644266043	25.487031568344666
51	20.139353259186702	23.69180109810272	28.519705967755733	27.64913967495485
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2685.0
1	2814.0
2	2943.0
3	3885.0
4	4827.0
5	3522.5
6	2218.0
7	2244.5
8	2271.0
9	2434.5
10	2598.0
11	2749.5
12	2901.0
13	3221.0
14	3541.0
15	3654.5
16	3768.0
17	3922.0
18	4076.0
19	4324.0
20	4572.0
21	4719.5
22	4867.0
23	5997.0
24	7127.0
25	9369.5
26	13399.0
27	15186.0
28	18326.5
29	21467.0
30	25407.0
31	29347.0
32	32823.5
33	36300.0
34	40806.5
35	45313.0
36	48956.5
37	52600.0
38	57144.5
39	61689.0
40	68859.5
41	76030.0
42	85266.0
43	94502.0
44	105260.0
45	116018.0
46	132868.5
47	149719.0
48	166144.0
49	182569.0
50	177476.0
51	172383.0
52	152298.5
53	132214.0
54	114698.5
55	97183.0
56	86886.5
57	76590.0
58	69043.0
59	61496.0
60	56514.0
61	51532.0
62	46715.0
63	41898.0
64	35799.5
65	29701.0
66	24646.0
67	19591.0
68	16353.0
69	13115.0
70	10993.5
71	8872.0
72	7403.5
73	5935.0
74	4870.0
75	3048.0
76	2291.0
77	1834.0
78	1377.0
79	1000.0
80	623.0
81	468.0
82	313.0
83	230.0
84	147.0
85	97.0
86	47.0
87	37.5
88	28.0
89	27.5
90	27.0
91	25.5
92	24.0
93	18.5
94	13.0
95	8.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1659954.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.88388525671855
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.86406823677542	21.176936840120703
2	12.12486000405868	7.246758502301313
3	4.715076460867124	4.2271441179962315
4	2.4205072632125533	2.8933664526759117
5	1.3948348712509966	2.084154262226729
6	0.8953215434393246	1.6053411763205356
7	0.6467970054873419	1.3530165247461015
8	0.5075588068402924	1.2134263315721816
9	0.42665183073814716	1.1475012918912926
>10	5.235957950776977	37.701305920309224
>50	0.6922638932255295	13.123100842781515
>100	0.06974319930623403	3.756274928950623
>500	0.003692284270475069	0.7708460991035285
>1k	0.002666649750898661	1.7008267090040954
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGC	4012	0.24169344451713723	No Hit
CTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGCT	3811	0.22958467523798853	No Hit
CCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGC	3223	0.19416200689898636	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2366	0.142534070221223	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2296	0.13831708589515132	No Hit
TCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGC	2209	0.13307597680417652	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCC	2059	0.12403958181973716	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1823	0.10982232037755263	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.27494737805987396	0.0
2	0.0	0.0	0.0	1.0306309692919202	0.0
3	0.0	0.0	0.0	1.4283528338737097	0.0
4	6.024263322959552E-5	0.0	0.0	1.912221663973821	0.0
5	6.024263322959552E-5	0.0	0.0	3.126351694083089	0.0
6	6.024263322959552E-5	0.0	0.0	3.9287835687013013	0.0
7	6.024263322959552E-5	0.0	0.0	4.745011006329091	0.0
8	6.024263322959552E-5	0.0	0.0	6.100108798195612	0.0
9	6.024263322959552E-5	0.0	0.0	6.6017492050984545	0.0
10	6.024263322959552E-5	0.0	0.0	7.875398956838563	0.0
11	6.024263322959552E-5	0.0	0.0	9.725450223319442	0.0
12	6.024263322959552E-5	0.0	0.0	11.086632521142151	0.0
13	1.2048526645919104E-4	0.0	0.0	11.620562979456057	0.0
14	1.2048526645919104E-4	0.0	0.0	11.84809940516424	0.0
15	1.2048526645919104E-4	0.0	0.0	12.247929761909065	0.0
16	1.2048526645919104E-4	0.0	0.0	13.094941185117177	0.0
17	1.2048526645919104E-4	0.0	0.0	14.186838912403596	0.0
18	1.8072789968878654E-4	0.0	0.0	15.329400694236105	0.0
19	1.8072789968878654E-4	0.0	0.0	16.038336002081987	0.0
20	1.8072789968878654E-4	0.0	0.0	16.717631934378904	0.0
21	1.8072789968878654E-4	0.0	0.0	17.569583253511844	0.0
22	1.8072789968878654E-4	0.0	0.0	18.46364417327227	0.0
23	6.626689655255507E-4	0.0	0.0	19.348066271715965	0.0
24	6.626689655255507E-4	0.0	0.0	20.056459395862777	0.0
25	6.626689655255507E-4	0.0	0.0	20.677982642892513	0.0
26	6.626689655255507E-4	0.0	0.0	21.259203568291653	0.0
27	7.229115987551462E-4	0.0	0.0	21.84921991814231	0.0
28	7.229115987551462E-4	0.0	0.0	22.445742472381763	0.0
29	7.229115987551462E-4	0.0	0.0	23.10768852630856	0.0
30	7.229115987551462E-4	0.0	0.0	23.82421440594137	0.0
31	7.229115987551462E-4	0.0	0.0	24.46772621409991	0.0
32	7.229115987551462E-4	0.0	0.0	25.078586515048006	0.0
33	7.229115987551462E-4	0.0	0.0	25.680289935745208	0.0
34	7.229115987551462E-4	0.0	0.0	26.29494552258677	0.0
35	7.229115987551462E-4	0.0	0.0	26.930023362093166	0.0
36	7.229115987551462E-4	0.0	0.0	27.534618429185386	0.0
37	7.229115987551462E-4	0.0	0.0	28.141502716340334	0.0
38	7.229115987551462E-4	0.0	0.0	28.746097783432553	0.0
39	7.229115987551462E-4	0.0	0.0	29.371416316355756	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCCCA	20	7.0341944E-4	45.0	34
CGTAAGG	255	0.0	43.235294	2
TTACGAG	80	0.0	42.1875	1
CCCTCGT	120	0.0	41.249996	14
GCGTAAG	155	0.0	40.64516	1
GCTATCG	45	1.9292202E-8	40.0	1
AGTACGG	160	0.0	39.375	2
CGTTTTT	1955	0.0	37.749363	1
CCGTAAG	60	1.5643309E-10	37.499996	1
TATCGAG	60	1.5643309E-10	37.499996	1
GTTAGCG	145	0.0	37.24138	1
ACAACGG	140	0.0	36.964287	2
ACGTTAG	55	2.750312E-9	36.81818	1
CGAATAT	130	0.0	36.346157	14
TCGATCA	155	0.0	36.29032	17
GCGCCTA	25	0.002107378	36.0	9
CCGCTAA	25	0.002107378	36.0	26
AACGGGA	575	0.0	36.0	4
GCGATAC	75	1.8189894E-12	36.0	9
CAGTCGA	25	0.002107378	36.0	31
>>END_MODULE