FastQCFastQC Report
Sat 18 Jun 2016
SRR3552402_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552402_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences837195
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCTGC35080.41901826933987907TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCTGCT30990.37016465697955675TruSeq Adapter, Index 13 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23590.2817742580880201No Hit
CCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCTGC23070.27556304086861483TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCC18010.21512311946440196No Hit
TCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCTGC16160.1930255197415178TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14840.1772585837230275No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11290.13485508155208764No Hit
ACTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCTGC10390.12410489790311695TruSeq Adapter, Index 13 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGTA453.8380676E-1045.041
TCGTTTA253.889895E-545.038
GTCGACG253.889895E-545.01
CGACGGT253.889895E-545.028
TCGGTTC1000.045.013
CTAACGG600.044.9999962
GCGTTAG800.042.18751
TATGGGT2400.041.2499964
GCGTAAG1050.040.7142871
CGTTAGG1500.040.5000042
CGTTTTT14750.039.6610151
ACGCATT403.4567165E-739.37517
CCGATAG403.4567165E-739.37511
CATAGCG403.4567165E-739.3751
ACTAACG403.4567165E-739.3751
TATAGCG403.4567165E-739.3751
AATAGCG403.4567165E-739.3751
CTCGGTT1150.039.13043212
GCAACGA356.246255E-638.5714311
CGCGCAA356.246255E-638.5714318
Produced by FastQC (version 0.11.5)